CN210514211U - Laser type thin-layer chromatography scanning imaging analysis device - Google Patents
Laser type thin-layer chromatography scanning imaging analysis device Download PDFInfo
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- CN210514211U CN210514211U CN201921072262.3U CN201921072262U CN210514211U CN 210514211 U CN210514211 U CN 210514211U CN 201921072262 U CN201921072262 U CN 201921072262U CN 210514211 U CN210514211 U CN 210514211U
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Abstract
The utility model discloses a laser type thin-layer chromatography scanning imaging analysis device, which is characterized by comprising a box body, a support plate, a laser light source mechanism and a light path system, wherein the support plate, the laser light source mechanism and the light path system are arranged in the box body; the outer side wall of the box body is provided with a control panel; the carrier plate is arranged at the bottom in the box body, and the optical path system comprises an objective lens, a beam splitter, a reflector, an emission filter module, a detector lens, a slit and a photomultiplier; the beam splitter is arranged on an excitation light path of the laser light source mechanism, the upper side and the lower side of the beam splitter are respectively provided with a reflector and an objective lens, the beam splitter, the reflector and the objective lens are coaxially arranged, an emergent light path of the reflector is along the horizontal direction, and an emergent light path of the reflector is sequentially provided with a detector lens, an emission light filter module, a slit and a photomultiplier. The utility model discloses sensitivity and detection accuracy improve greatly, have realized high flux analysis, and application scope is extensive.
Description
Technical Field
The utility model relates to a detect technical field, concretely relates to laser type thin-layer chromatography scanning image analysis device.
Background
The detection mode of the existing thin-layer chromatography scanner or the thin-layer chromatography imaging system on the market is single. The thin-layer chromatography scanner can only perform linear scanning of a certain column of spread spots along the spreading direction, and although the thin-layer chromatography imaging system can perform full-plate detection, the sensitivity and accuracy are low because the thin-layer chromatography imaging system uses a common lamp tube as a light source and a CCD as a detector, and the thin-layer chromatography imaging system can only be used for fluorescence detection of limited varieties.
Therefore, it is highly desirable to develop a novel thin-layer chromatography scanning imaging system with a wide application range and high sensitivity and accuracy.
Disclosure of Invention
The utility model aims at overcoming the above-mentioned not enough, providing a laser type thin layer chromatography scanning image analysis device, sensitivity and detection accuracy improve greatly, have realized high flux analysis, and application scope is extensive.
In order to achieve the above object, the present invention provides a laser thin-layer chromatography scanning imaging analyzer, which comprises a box, and a support plate, a laser light source mechanism and a light path system arranged in the box; the outer side wall of the box body is provided with a control panel, and the control panel is respectively in control connection with the laser light source mechanism and the light path system;
the support plate is arranged at the bottom in the box body, and a microporous plate chassis or a thin-layer plate pressing strip or a gel plate pressing strip is detachably arranged on the upper end surface of the support plate;
a partition board is arranged in the box body, the laser light source mechanism and the optical path system are arranged on the partition board, and the optical path system comprises an objective lens, a beam splitter, a reflector, an emission filter module, a detector lens, a slit and a photomultiplier; the beam splitter is arranged on an excitation light path of the laser light source mechanism, the reflector and the objective lens are respectively arranged on the upper side and the lower side of the beam splitter, the reflector and the objective lens are coaxially arranged, an emergent light path of the reflector is arranged along the horizontal direction, and the emergent light path of the reflector is sequentially provided with the detector lens, the emission light filter module, the slit and the photomultiplier tube;
the laser type thin-layer chromatography scanning imaging analysis device further comprises a data processing device arranged outside the box body, and the data processing device is electrically connected with the photomultiplier.
Furthermore, the laser light source mechanism comprises a linear slide rail, a movement driving mechanism and a laser tube set, the linear slide rail is arranged on the partition plate, the laser tube set is arranged on the linear slide rail in a sliding mode through a sliding block, and the movement driving mechanism is used for driving the laser tube set to slide on the linear slide rail.
Further, the motion actuating mechanism includes step motor, synchronous pulley, hold-in range and axis of rotation, step motor is fixed in on the baffle, one end is rotated and is provided with in the linear slide rail the axis of rotation, the other end is rotated and is provided with in the linear slide rail synchronous pulley, the axis of rotation the last tensioning of synchronous pulley is provided with the hold-in range, the step motor output shaft with synchronous pulley connects, the hold-in range passes the slider.
Furthermore, the emission optical filter module comprises a filter wheel for bearing a plurality of optical filters and a synchronous motor, and the filter wheel is connected with an output shaft of the synchronous motor.
Further, the data processing device is a computer.
Compared with the prior art, the beneficial effects of the utility model reside in that:
1. the utility model relates to a laser type thin-layer chromatography scanning image analysis device adopts laser as light source, photomultiplier as the detector, and sensitivity and detection accuracy improve greatly, have realized high flux analysis, and application scope is extensive.
2. The laser tube group in the laser type thin-layer chromatography scanning imaging analysis device can quickly and conveniently complete position adjustment through the driving of the motion driving mechanism,
3. the utility model relates to a detachable micropore board chassis or thin-layer board layering or gel board layering of installing on carrying board up end among laser type thin-layer chromatography scanning imaging analysis device satisfies different detection methods' demand.
Drawings
Fig. 1 is the appearance structure diagram of the laser thin-layer chromatography scanning imaging analyzer of the present invention.
Fig. 2 is a front view of the internal structure of fig. 1.
Fig. 3 is the schematic view of the overlooking internal structure of the box body in the laser thin-layer chromatography scanning imaging analysis device of the present invention.
Fig. 4 is a schematic view of the laser source mechanism in the laser thin-layer chromatography scanning imaging analyzer.
Fig. 5 is a schematic structural diagram of a carrier plate in a laser thin-layer chromatography scanning imaging analyzer of the present invention.
Fig. 6 is a schematic diagram of a detachable microplate chassis installed on a support plate of the laser thin-layer chromatography imaging analysis device.
Fig. 7 is a schematic structural diagram of the laser thin-layer chromatography imaging analyzer with a thin-layer plate pressing bar detachably mounted on the support plate.
Fig. 8 is a schematic structural diagram of the laser thin-layer chromatography imaging analyzer with gel-plate pressing strips detachably mounted on the carrier plate.
Fig. 9 is a schematic structural diagram of a motion driving mechanism in a laser thin-layer chromatography scanning imaging analysis apparatus of the present invention.
The correspondence between each mark and the part name is as follows:
the device comprises a box body 1, a carrier plate 2, a bottom plate 201, a top plate 202, an X-axis slide rail 203, a Y-axis slide rail 204, an X-axis driving mechanism 205, a Y-axis driving mechanism 206, a third positioning hole 207, a second positioning hole 208, a first positioning hole 209, a laser light source mechanism 3, a linear slide rail 301, a motion driving mechanism 302, a laser tube group 303, a stepping motor 304, a synchronous pulley 305, a synchronous belt 306, a rotating shaft 307, a slider 308, an optical path system 4, an objective lens 401, a beam splitter 402, a reflective mirror 403, an emission filter module 404, a detector lens 405, a slit 406, a photomultiplier 407, a filter wheel 408, a synchronous motor 409, a microplate chassis 5, a thin-layer plate pressing strip 6, a gel plate pressing strip 7, a partition plate 8, a data processing.
Detailed Description
In order to make the technical means, the inventive features, the objectives and the functions of the present invention easy to understand, the present invention will be further described with reference to the following specific drawings.
As shown in fig. 1 and fig. 2, a laser thin-layer chromatography scanning imaging analysis apparatus includes a box 1, a carrier plate 2 disposed in the box 1, a laser light source mechanism 3, and an optical path system 4; the outer side wall of the box body 1 is provided with a control panel 10, and the control panel 10 is respectively connected with the laser light source mechanism 3 and the optical path system 4 in a control mode.
The support plate 2 is arranged at the bottom in the box body 1, and a microporous plate chassis 5 or a thin-layer plate pressing strip 6 or a gel plate pressing strip 7 is detachably arranged on the upper end surface of the support plate 2.
As shown in fig. 3 and 4, a partition plate 8 is provided in the case 1, the laser light source mechanism 3 and the optical path system 4 are both provided on the partition plate 8, and the optical path system 4 includes an objective lens 401, a beam splitter 402, a reflective mirror 403, an emission filter module 404, a detector lens 405, a slit 406, and a photomultiplier 407; the beam splitter 402 is arranged on an excitation light path of the laser light source mechanism 3, a reflector 403 and an objective lens 401 are respectively arranged on the upper side and the lower side of the beam splitter 402, the reflector 403 and the objective lens 401 are coaxially arranged, an emergent light path of the reflector 403 is along the horizontal direction, and an emergent light path of the reflector 403 is sequentially provided with a detector lens 405, an emission filter module 404, a slit 406 and a photomultiplier 407.
As shown in fig. 1 and fig. 2, the laser thin-layer chromatography scanning imaging analyzer of the present embodiment further includes a data processing device 9 disposed outside the box 1, and the data processing device 9 is electrically connected to the photomultiplier 407.
As shown in fig. 2 and fig. 3, the laser light source mechanism 3 in the laser thin-layer chromatography scanning imaging analysis apparatus of this embodiment includes a linear slide rail 301, a movement driving mechanism 302, and a laser tube set 303, wherein the linear slide rail 301 is disposed on the partition board 8, the laser tube set 303 is slidably disposed on the linear slide rail 301 through a slider 308, and the movement driving mechanism 302 is configured to drive the laser tube set 303 to slide on the slide rail.
As shown in fig. 9, the movement driving mechanism 302 in the laser thin-layer chromatography scanning imaging analysis apparatus of this embodiment includes a stepping motor 304, a synchronous pulley 305, a synchronous belt 306, and a rotating shaft 307, the stepping motor 304 is fixed on the partition board, the rotating shaft 307 is rotatably disposed at one end in the linear slide rail 301, the synchronous pulley 305 is rotatably disposed at the other end in the linear slide rail 301, an output shaft of the stepping motor 304 is connected with the synchronous pulley 305, the synchronous belt 306 is tensioned on the rotating shaft 307 and the synchronous pulley 305, and the synchronous belt 306 passes through the sliding block 308.
The emission filter module 404 of the laser thin-layer chromatography imaging analyzer of this embodiment includes a filter wheel 408 carrying a plurality of filters and a synchronous motor 409, and the filter wheel 408 is connected to an output shaft of the synchronous motor 409.
The data processing device 9 in the laser type thin-layer chromatography scanning imaging analysis device of the embodiment is a computer.
As shown in fig. 5, the carrier plate 2 in this embodiment includes a bottom plate 201, a top plate 202, an X-axis slide rail 203, a Y-axis slide rail 204, an X-axis driving mechanism 205, and a Y-axis driving mechanism 206;
two X-axis slide rails 203 are arranged at the bottom in the box body 1 in parallel, first slide blocks are arranged on the two X-axis slide rails 203 in a sliding manner, and the lower end surface of the bottom plate 201 is fixedly connected with the first slide blocks on the two X-axis slide rails 203; two Y-axis slide rails 204 are arranged on the upper end face of the bottom plate 201 in parallel, second slide blocks are arranged on the two Y-axis slide rails 204 in a sliding manner, and the lower end face of the top plate 202 is fixedly connected with the second slide blocks on the two Y-axis slide rails 204;
the X-axis slide rail 203 and the Y-axis slide rail 204 are arranged vertically to each other; the X-axis driving mechanism 205 is configured to drive the bottom plate 201 to slide on the two X-axis slide rails 203, so that the bottom plate 201 moves linearly along the X-axis direction; the Y-axis driving mechanism 206 is used for sliding the top plate 202 on the two Y-axis slide rails 204 to make the top plate 202 move linearly along the Y-axis direction.
As shown in fig. 6, 7 and 8, the microplate chassis 5, the thin-layer plate bead 6 or the gel plate bead 7 is detachably mounted on the upper end surface of the top plate 202. The microplate chassis 5 or the thin-layer plate bead 6 or the gel plate bead 7 are connected with the top plate 202 by screws.
The top plate 202 is further provided with three positioning holes which are linearly arranged and respectively comprise a first positioning hole 209, a second positioning hole 208 and a third positioning hole 207, the second positioning hole 208 is arranged between the third positioning hole 207 and the first positioning hole 209, the linear distance between one end, close to the first positioning hole 209, of the top plate 202 and the first positioning hole 209 is 50mm, the linear distance between the second positioning hole 208 and one end, close to the first positioning hole 209, of the top plate 202 is 100mm, and the linear distance between the first positioning hole 209 and one end, close to the first positioning hole 209, of the top plate 202 is 200 mm.
After the thin-layer plate pressing strip 6 or the gel plate pressing strip 7 is fixed on the top plate 202, one end of the thin-layer plate pressing strip 6 or one end of the gel plate pressing strip 7 covers the third positioning hole 207, the second positioning hole 208 or the first positioning hole 209.
The positions of the thin-layer plate pressing strips 6 or the gel plate pressing strips 7 covering the third positioning holes 207, the second positioning holes 208 or the first positioning holes 209 are adjusted, so that the placing requirements of gel plates of different models are met.
The top plate 202 can move along the directions of an X axis and/or a Y axis under the driving of the X axis driving mechanism 205 and the Y axis driving mechanism 206, so that the worker can conveniently and flexibly realize the position adjustment of the sample by adjusting the position of the support plate 2; in addition, the detection of the microporous plate, the detection of the thin-layer plate and the detection of the gel plate can be compatible at the same time, the analysis range is expanded, the use of workers is facilitated, the replacement of different carrier plates 2 when different detection modes are switched is avoided, and the working efficiency is improved.
The X-axis driving mechanism 205 in this embodiment includes an X-axis stepping motor and a first ball screw, the X-axis stepping motor and the first ball screw are both installed at the bottom in the box 1, the X-axis stepping motor is connected with the first ball screw to drive the first ball screw to rotate, a first screw nut is sleeved on the first ball screw, and the first screw nut is fixedly connected with a first slider through a slider connecting member.
The Y-axis driving mechanism 206 in this embodiment includes a Y-axis stepping motor and a second ball screw, the Y-axis stepping motor and the second ball screw are both installed on the upper end surface of the bottom plate 201, the Y-axis stepping motor is connected with the second ball screw to drive the second ball screw to rotate, a second screw nut is sleeved on the second ball screw, and the second screw nut is fixedly connected with a second slider through a slider connecting member.
In this embodiment, a laser-based thin-layer chromatography scanning imaging analysis apparatus uses multi-color laser as an excitation light source, when the laser-based thin-layer chromatography scanning imaging analysis apparatus in this embodiment is applied, a sample is placed on a support plate 2, then position adjustment of the sample is realized by driving of an X-axis driving mechanism 205 and a Y-axis driving mechanism 206, the sample is located under an objective lens 401, a fluorescence is emitted by irradiation of a laser tube set 303, in the sample focused by a laser light source, if there is fluorescence in the sample, the fluorescence emitted by the sample is collected by the objective lens 401, and is captured by a photomultiplier 407 after sequentially passing through a reflective mirror 403, a detector lens 405, an emission filter module 404, and a slit 406, if the fluorescence generated by the fluorescence is captured by the photomultiplier 407, a signal of each photon is expanded by the photomultiplier 407 and converted into a digital signal, and an image representing the signal intensity of each pixel region is generated, the data processing means 9 further processes the images to determine the fluorescence intensity of each signal point in each channel and generates a data table relating signal point-intensity values.
To sum up, the utility model relates to a laser type thin layer chromatography scanning image analysis device sensitivity and detection accuracy improve greatly, have realized high flux analysis, convenient to use, and application scope is extensive, and detection efficiency is high.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A laser type thin-layer chromatography scanning imaging analysis device is characterized by comprising a box body, a support plate, a laser light source mechanism and a light path system, wherein the support plate, the laser light source mechanism and the light path system are arranged in the box body; the outer side wall of the box body is provided with a control panel, and the control panel is respectively in control connection with the laser light source mechanism and the light path system;
the support plate is arranged at the bottom in the box body, and a microporous plate chassis or a thin-layer plate pressing strip or a gel plate pressing strip is detachably arranged on the upper end surface of the support plate;
a partition board is arranged in the box body, the laser light source mechanism and the optical path system are arranged on the partition board, and the optical path system comprises an objective lens, a beam splitter, a reflector, an emission filter module, a detector lens, a slit and a photomultiplier; the beam splitter is arranged on an excitation light path of the laser light source mechanism, the reflector and the objective lens are respectively arranged on the upper side and the lower side of the beam splitter, the reflector and the objective lens are coaxially arranged, an emergent light path of the reflector is arranged along the horizontal direction, and the emergent light path of the reflector is sequentially provided with the detector lens, the emission light filter module, the slit and the photomultiplier tube;
the laser type thin-layer chromatography scanning imaging analysis device further comprises a data processing device arranged outside the box body, and the data processing device is electrically connected with the photomultiplier.
2. The laser-based thin layer chromatography imaging analysis device as claimed in claim 1, wherein the laser source mechanism comprises a linear slide rail, a motion driving mechanism and a laser tube set, the linear slide rail is disposed on the partition board, the laser tube set is slidably disposed on the linear slide rail through a sliding block, and the motion driving mechanism is configured to drive the laser tube set to slide on the linear slide rail.
3. The laser-based thin layer chromatography imaging analyzer as claimed in claim 2, wherein the motion driving mechanism comprises a stepping motor, a synchronous pulley, a synchronous belt and a rotating shaft, the stepping motor is fixed on the partition plate, the rotating shaft is rotatably disposed at one end of the linear slide rail, the synchronous pulley is rotatably disposed at the other end of the linear slide rail, the synchronous belt is tensioned on the rotating shaft and the synchronous pulley, the output shaft of the stepping motor is connected with the synchronous pulley, and the synchronous belt passes through the sliding block.
4. The laser-based thin layer chromatography imaging analysis device of claim 1, wherein the emission filter module comprises a filter wheel carrying a plurality of filters and a synchronous motor, the filter wheel being connected to the output shaft of the synchronous motor.
5. A laser type thin layer chromatography imaging analysis apparatus as claimed in claim 1, wherein said data processing means is a computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921072262.3U CN210514211U (en) | 2019-07-10 | 2019-07-10 | Laser type thin-layer chromatography scanning imaging analysis device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921072262.3U CN210514211U (en) | 2019-07-10 | 2019-07-10 | Laser type thin-layer chromatography scanning imaging analysis device |
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| CN210514211U true CN210514211U (en) | 2020-05-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114993967A (en) * | 2022-04-26 | 2022-09-02 | 上海杜凯生物科技有限公司 | Light path micropore plate analysis device of light absorption ELIASA |
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2019
- 2019-07-10 CN CN201921072262.3U patent/CN210514211U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114993967A (en) * | 2022-04-26 | 2022-09-02 | 上海杜凯生物科技有限公司 | Light path micropore plate analysis device of light absorption ELIASA |
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