CN210438734U - Low-temperature ultrasonic cell crusher - Google Patents

Low-temperature ultrasonic cell crusher Download PDF

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Publication number
CN210438734U
CN210438734U CN201921317567.6U CN201921317567U CN210438734U CN 210438734 U CN210438734 U CN 210438734U CN 201921317567 U CN201921317567 U CN 201921317567U CN 210438734 U CN210438734 U CN 210438734U
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China
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fixedly connected
ultrasonic
mounting
base
installation
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CN201921317567.6U
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Inventor
王彦辉
李自波
刘兵
李国辉
焦金英
张宁
孟祥�
李冰
姬星宇
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Shangqiu Meilan Biological Engineering Co ltd
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Shangqiu Meilan Biological Engineering Co ltd
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Abstract

The utility model discloses a low temperature ultrasonic wave cell crusher relates to cell disruption technical field, the on-line screen storage device comprises a base, the top of base is equipped with the mounting disc, the top of mounting disc is equipped with the fixed disk, the top middle part of fixed disk is equipped with broken bucket, the both sides of broken bucket all are equipped with clamping machine and construct, the equal fixedly connected with bracing piece in base top both sides, the inboard upper end fixedly connected with installation piece of bracing piece, the top of broken bucket is equipped with ultrasonic generrator, ultrasonic generrator's bottom fixedly connected with ultrasonic probe, the equal fixedly connected with installation pole in both sides of ultrasonic generrator, the mounting disc passes through back force spring and fixed disk fixed connection. The utility model discloses a set up clamping mechanism, again under the mating reaction between supporting shoe, splint, through-hole, dead lever, stopper and compression spring, not only can fix broken bucket, made things convenient for the installation and the dismantlement of broken bucket, when wasing or getting the material, labour saving and time saving more.

Description

Low-temperature ultrasonic cell crusher
Technical Field
The utility model relates to a cell disruption technical field specifically is a low temperature ultrasonic wave cell disruption machine.
Background
The cell disruption technology is a technology which utilizes external force to destroy cell membranes and cell walls so as to release cell contents including target product components. When a certain protein is separated and purified by cell disruption, the protein is firstly released from tissues or cells and is kept in the original natural state without losing activity. Appropriate methods are used to disrupt the tissue and cells. The cell is difficult to be broken in different organisms or tissues of different parts of the same organism, the used method is different, for example, cell membranes of animal organs are fragile and easy to be broken, and plants and microorganisms have firmer cell walls consisting of cellulose and hemicellulose.
Need use the cell breaker in the cell disruption experiment, the cell breaker among the prior art can not take off broken bucket, and is very troublesome when wasing or getting the material, influences work efficiency, and the inconvenient broken bucket of installing the model difference of current cell breaker in addition, and the practicality is poor.
Therefore, it is necessary to invent a low-temperature ultrasonic cell crusher to solve the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a low temperature ultrasonic wave cell crusher to the cell crusher among the prior art who proposes in solving above-mentioned background art can not say that broken bucket takes off, is very troublesome and the different broken bucket of the inconvenient installation model of cell crusher when wasing or getting the material, the poor problem of practicality.
In order to achieve the above purpose, the utility model discloses a following technical scheme realizes:
a low-temperature ultrasonic cell crusher comprises a base, wherein an installation disc is arranged above the base, a fixed disc is arranged above the installation disc, a crushing barrel is arranged in the middle of the top end of the fixed disc, clamping mechanisms are arranged on two sides of the crushing barrel, supporting rods are fixedly connected to two sides of the top end of the base, an installation block is fixedly connected to the upper end of the inner side of each supporting rod, an ultrasonic generator is arranged above the crushing barrel, an ultrasonic probe is fixedly connected to the bottom of the ultrasonic generator, installation rods are fixedly connected to two sides of the ultrasonic generator, and the installation disc is fixedly connected with the fixed disc through a return spring;
an adjusting groove is formed in the middle of the top end of the base, a fixing column is movably connected in the adjusting groove, the top end of the fixing column is fixedly connected with the mounting plate, a threaded hole is formed in the fixing column, a threaded rod is connected with the threaded hole in an internal thread mode, the bottom of the threaded rod, penetrating out of the adjusting groove, is connected with an output shaft of the servo motor, and a bearing is arranged between the threaded rod and the base;
the clamping mechanism comprises a supporting block and a clamping plate, the supporting block is fixedly connected with a fixed disc, a through hole is formed in the middle of the supporting block, a fixing rod is movably connected in the through hole, one end of the fixing rod is fixedly connected with the clamping plate, the other end of the fixing rod is fixedly connected with a limiting block, a compression spring is sleeved on the outer side of one end of the fixing rod, and a circulation channel is formed in the clamping plate;
the ultrasonic generator is characterized in that a mounting groove is formed in one side of the mounting block, a mounting column is fixedly connected in the mounting groove, and a mounting hole is formed in one end, far away from the ultrasonic generator, of the mounting rod.
Optionally, the crushing barrel corresponds to the ultrasonic probe, and the crushing barrel is attached to the clamping plate.
Optionally, the return spring is provided with a plurality of return springs, and the return springs are distributed in an annular array.
Optionally, guide holes are formed in two sides of the top end of the base, guide rods are movably connected in the guide holes, and the top ends of the guide rods are fixedly connected with the mounting disc.
Optionally, one end of the compression spring is fixedly connected with the supporting block, and the other end of the compression spring is fixedly connected with the clamping plate.
Optionally, the clamping plate is arc-shaped, and the circulation channel is U-shaped.
Optionally, the mounting rod is adapted to the mounting groove, and the mounting hole is adapted to the mounting post.
The utility model discloses a technological effect and advantage:
1. the utility model discloses a set up clamping mechanism, again at the supporting shoe, splint, the through-hole, the dead lever, under the mating reaction between stopper and the compression spring, not only can fix broken bucket, the installation and the dismantlement of broken bucket have been made things convenient for, when wasing or getting the material, labour saving and time saving more, and can the broken bucket of the different diameters of clamping, therefore, the clothes hanger is strong in practicability, through the mounting groove, the erection column, cooperation setting between installation pole and the mounting hole, the installation and the dismantlement of ultrasonic generrator have been made things convenient for, it is more laborsaving in the assembly, through setting up the return spring, the absorbing effect has been played.
2. The utility model discloses, through the cooperation setting between adjustment tank, fixed column, screw hole, threaded rod, bearing and the servo motor, be convenient for adjust the upper and lower position of broken bucket, make broken bucket and ultrasonic probe's position adaptation.
Drawings
FIG. 1 is a schematic front view of the structure of the present invention;
FIG. 2 is a schematic cross-sectional view of the base structure of the present invention;
FIG. 3 is a schematic top sectional view of the clamping mechanism of the present invention;
FIG. 4 is a schematic cross-sectional view of the splint structure of the present invention;
fig. 5 is a schematic cross-sectional view of the mounting block structure of the present invention;
fig. 6 is a schematic top view of the mounting block structure of the present invention.
In the figure: 1. a base; 2. mounting a disc; 3. fixing the disc; 4. a clamping mechanism; 5. a crushing barrel; 6. a support bar; 7. mounting blocks; 8. an ultrasonic generator; 9. an ultrasonic probe; 10. a return spring; 11. an adjustment groove; 12. fixing a column; 13. a threaded hole; 14. a threaded rod; 15. a bearing; 16. a servo motor; 17. a guide hole; 18. a guide bar; 19. a support block; 20. a splint; 21. a through hole; 22. fixing the rod; 23. a limiting block; 24. a compression spring; 25. a circulation channel; 26. mounting grooves; 27. mounting a column; 28. mounting a rod; 29. and (7) installing holes.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; may be a mechanical connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
The utility model provides a low-temperature ultrasonic cell crusher as shown in figures 1-6, which comprises a base 1 and is shown in figure 1, wherein a mounting disc 2 is arranged above the base 1, a fixed disc 3 is arranged above the mounting disc 2, a crushing barrel 5 is arranged in the middle of the top end of the fixed disc 3, clamping mechanisms 4 are arranged on two sides of the crushing barrel 5, supporting rods 6 are fixedly connected on two sides of the top end of the base 1, a mounting block 7 is fixedly connected on the upper end of the inner side of each supporting rod 6, an ultrasonic generator 8 is arranged above the crushing barrel 5, an ultrasonic probe 9 is fixedly connected on the bottom of the ultrasonic generator 8, the crushing barrel 5 corresponds to the ultrasonic probe 9, mounting rods 28 are fixedly connected on two sides of the ultrasonic generator 8, the mounting disc 2 is fixedly connected with the fixed disc 3 through a return spring 10, a plurality of return springs 10 are arranged, the return springs 10 are distributed in an annular array, and by arranging the return springs, the shock absorption function is realized;
as shown in fig. 2, an adjusting groove 11 is formed in the middle of the top end of a base 1, a fixing column 12 is movably connected in the adjusting groove 11, the top end of the fixing column 12 is fixedly connected with an installation plate 2, a threaded hole 13 is formed in the fixing column 12, a threaded rod 14 is connected in the threaded hole 13 in a threaded manner, the bottom of the threaded rod 14 penetrates through the bottom of the adjusting groove 11 and is connected with an output shaft of a servo motor 16, and a bearing is arranged between the threaded rod 14 and the base 1;
as shown in fig. 3 and 4, the clamping mechanism 4 comprises a supporting block 19 and a clamping plate 20, the supporting block 19 is fixedly connected with the fixed disk 3, the clamping plate 20 is arc-shaped, the crushing barrel 5 is attached to the clamping plate 20, a through hole 21 is formed in the middle of the supporting block 19, a fixing rod 22 is movably connected in the through hole 21, one end of the fixing rod 22 is fixedly connected with the clamping plate 20, the other end of the fixing rod 22 is fixedly connected with a limiting block 23, a compression spring 24 is sleeved outside one end of the fixing rod 22, one end of the compression spring 24 is fixedly connected with the supporting block 19, and the other end of the compression spring 24 is fixedly connected with the clamping plate 20, by arranging the clamping mechanism 4, under the matching action among the supporting block 19, the clamping plate 20, the through hole 21, the fixing rod 22, the limiting block 23 and the compression spring 24, the crushing barrel 5 can be, the crushing barrel clamp has the advantages that time and labor are saved, the crushing barrels 5 with different diameters can be clamped, the practicability is high, the circulating channel 25 is formed in the clamping plate 20, and the circulating channel 25 is U-shaped;
as shown in fig. 5 and 6, one side of the mounting block 7 is provided with a mounting groove 26, a mounting post 27 is fixedly connected in the mounting groove 26, one end of the mounting rod 28, which is far away from the ultrasonic generator 8, is provided with a mounting hole 29, the mounting rod 28 is matched with the mounting groove 26, the mounting hole 29 is matched with the mounting post 27, and the mounting hole 29 is matched with the mounting post 27 through the mounting groove 26, the mounting post 27, the mounting rod 28 and the mounting hole 29, so that the mounting and the dismounting of the ultrasonic generator 8 are facilitated, and the assembly is more labor-saving.
The electrical components presented in the document are all electrically connected with an external master controller and 220V mains, and the master controller can be a conventional known device controlled by a computer or the like.
This practical theory of operation: when the ultrasonic crushing device is used, the crushing barrel 5 is placed between the two clamping mechanisms 4, then the mounting rods 28 at two ends of the ultrasonic generator 8 are placed in the mounting grooves 26 in the mounting blocks 7, the mounting columns 27 enter the mounting holes 29, then the servo motor 16 is started, the servo motor 16 drives the fixing columns 12 to move upwards through the threaded rods 14, the fixing columns 12 drive the crushing barrel 5 to move upwards through the mounting disc 2 and the fixing disc 3, the crushing barrel 5 and the ultrasonic probe 9 are enabled to be in proper positions, then the ultrasonic generator 8 is started, ultrasonic crushing is started, then two ports at the upper end of the circulating channel 25 are connected with the upper water inlet pipe and the water outlet pipe, and a water source is used for introducing water so as to adjust the temperature in the crushing barrel 5.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims (7)

1. The utility model provides a low temperature ultrasonic wave cell breaker, includes base (1), its characterized in that: the ultrasonic vibration testing machine is characterized in that an installation disc (2) is arranged above the base (1), a fixed disc (3) is arranged above the installation disc (2), a crushing barrel (5) is arranged in the middle of the top end of the fixed disc (3), clamping mechanisms (4) are arranged on two sides of the crushing barrel (5), supporting rods (6) are fixedly connected to two sides of the top end of the base (1), an installation block (7) is fixedly connected to the upper end of the inner side of each supporting rod (6), an ultrasonic generator (8) is arranged above the crushing barrel (5), an ultrasonic probe (9) is fixedly connected to the bottom of the ultrasonic generator (8), installation rods (28) are fixedly connected to two sides of the ultrasonic generator (8), and the installation disc (2) is fixedly connected with the fixed disc (3) through a return spring (10);
an adjusting groove (11) is formed in the middle of the top end of the base (1), a fixing column (12) is movably connected in the adjusting groove (11), the top end of the fixing column (12) is fixedly connected with the mounting plate (2), a threaded hole (13) is formed in the fixing column (12), a threaded rod (14) is connected to the threaded hole (13) in an internal thread mode, the bottom of the threaded rod (14) penetrates through the adjusting groove (11) and is connected with an output shaft of a servo motor (16), and a bearing is arranged between the threaded rod (14) and the base (1);
the clamping mechanism (4) comprises a supporting block (19) and a clamping plate (20), the supporting block (19) is fixedly connected with a fixed disc (3), a through hole (21) is formed in the middle of the supporting block (19), a fixing rod (22) is movably connected in the through hole (21), one end of the fixing rod (22) is fixedly connected with the clamping plate (20), the other end of the fixing rod (22) is fixedly connected with a limiting block (23), a compression spring (24) is sleeved on the outer side of one end of the fixing rod (22), and a circulating channel (25) is formed in the clamping plate (20);
mounting groove (26) have been seted up to one side of installation piece (7), fixedly connected with erection column (27) in mounting groove (26), mounting hole (29) have been seted up to the one end that ultrasonic generator (8) were kept away from in installation pole (28).
2. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
the crushing barrel (5) corresponds to the ultrasonic probe (9), and the crushing barrel (5) is attached to the clamping plate (20).
3. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
the number of the return springs (10) is multiple, and the return springs (10) are distributed in an annular array.
4. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
guiding hole (17) have all been seted up to the top both sides of base (1), swing joint has guide bar (18) in guiding hole (17), the top and mounting disc (2) fixed connection of guide bar (18).
5. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
one end of the compression spring (24) is fixedly connected with the supporting block (19), and the other end of the compression spring (24) is fixedly connected with the clamping plate (20).
6. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
the clamping plate (20) is arc-shaped, and the circulating channel (25) is U-shaped.
7. The cryogenic ultrasonic cell disruptor of claim 1, wherein:
the mounting rod (28) is matched with the mounting groove (26), and the mounting hole (29) is matched with the mounting column (27).
CN201921317567.6U 2019-08-14 2019-08-14 Low-temperature ultrasonic cell crusher Active CN210438734U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921317567.6U CN210438734U (en) 2019-08-14 2019-08-14 Low-temperature ultrasonic cell crusher

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921317567.6U CN210438734U (en) 2019-08-14 2019-08-14 Low-temperature ultrasonic cell crusher

Publications (1)

Publication Number Publication Date
CN210438734U true CN210438734U (en) 2020-05-01

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CN201921317567.6U Active CN210438734U (en) 2019-08-14 2019-08-14 Low-temperature ultrasonic cell crusher

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110339211A (en) * 2019-08-14 2019-10-18 商丘美兰生物工程有限公司 A kind of method of quick production transfer factor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110339211A (en) * 2019-08-14 2019-10-18 商丘美兰生物工程有限公司 A kind of method of quick production transfer factor

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Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: Low temperature ultrasonic cell crusher

Effective date of registration: 20201009

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2020980006651

PE01 Entry into force of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20211011

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2020980006651

PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: Low temperature ultrasonic cell crusher

Effective date of registration: 20211013

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2021980010624

PE01 Entry into force of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20220915

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2021980010624

PC01 Cancellation of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: Low temperature ultrasonic cell crusher

Effective date of registration: 20220916

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2022980015417

PC01 Cancellation of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20230918

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2022980015417

PE01 Entry into force of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: Low temperature ultrasonic cell crusher

Effective date of registration: 20230921

Granted publication date: 20200501

Pledgee: Bank of China Limited by Share Ltd. Zhecheng branch

Pledgor: SHANGQIU MEILAN BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: Y2023980057629