CN210401186U - Mouse in vitro spinal cord and brachial plexus fixator - Google Patents
Mouse in vitro spinal cord and brachial plexus fixator Download PDFInfo
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- CN210401186U CN210401186U CN201921268223.0U CN201921268223U CN210401186U CN 210401186 U CN210401186 U CN 210401186U CN 201921268223 U CN201921268223 U CN 201921268223U CN 210401186 U CN210401186 U CN 210401186U
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Abstract
The in-vitro spinal cord and brachial plexus fixer for the mouse comprises a spinal cord fixing component and a nerve fixing component, wherein the spinal cord fixing component comprises a spinal cord base, a raised ridge is fixed in the middle of the spinal cord base, the height of the ridge is smaller than 1mm, and fixing holes are formed in the near end of the ridge and the spinal cord base; the nerve fixing component comprises a nerve base, the left side part of the nerve base is of a tenon structure matched with the mortise, more than two groups of nerve fixing sub-components are arranged on the nerve base, and the nerve fixing sub-components are arranged side by side left and right; to sum up, the utility model discloses a dead lever and ladder groove can be fixed nerve on nerve base, and mortise and tenon structure on mortise and tenon groove on the spinal cord base and the nerve base forms mortise and tenon structure for the two both can the exclusive use, can jointly use again, and overall structure reasonable in design can fix mouse separation spinal cord and nerve sample at its original anatomical position fast, prevents that the transparent tissue that handles probably leads to of light from warping the problem.
Description
Technical Field
The utility model belongs to the technical field of the experiment in neurobiology field, concretely relates to mouse separation spinal cord and brachial plexus nerve fixer.
Background
Morphological studies have played an important role in the field of neuroscience. Although a traditional method for performing histological sectioning and imaging and then performing computer three-dimensional reconstruction can acquire a three-dimensional high-resolution image of a large biological sample, the operation process is time-consuming and labor-consuming, and tissue deformation is difficult to avoid in the sectioning process, so that the problems of difficult three-dimensional reconstruction, image distortion after reconstruction, tissue information loss and the like are caused. The light transparent imaging technology brings a new opportunity for neuroscience research, and makes the whole tissue transparent through a chemical reagent, so that the optical scattering in the tissue is reduced, the imaging depth is greatly increased, and further the deep tissue imaging is facilitated. However, if the spinal cord or nerves are over-pulled during drawing of material or damaged before drawing of material and are not properly fixed before the light-clearing treatment, some light-clearing methods (e.g., 3 DISCO) may aggravate the anisotropic shrinkage of the tissue, which in turn may lead to severe deformation of these isolated tissues during the light-clearing treatment.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a solve the weak point among the prior art, provide a mouse separation spinal cord and brachial plexus nerve fixer, this fixer can fix mouse separation spinal cord and nerve sample at its original anatomical position fast, prevents the tissue deformation problem that light transparency processing probably leads to, and then acquires more accurate histology information when helping optical imaging.
In order to solve the technical problem, the utility model adopts the following technical scheme: the fixer for the isolated spinal cord and brachial plexus of the mouse comprises a spinal cord fixing component and a nerve fixing component; the spinal cord fixing component comprises a spinal cord base, wherein a raised ridge is fixed in the middle of the spinal cord base, at least one first fixing hole is formed in the near end of the ridge, and at least one second fixing hole is formed in the spinal cord base and positioned on the left side and the right side of the first fixing hole respectively;
a needle threading device is fixed in the middle of the near side of the spinal cord base through a support rod and is positioned above the middle of the near side of the spinal cord base, a plurality of guide holes are formed in the needle threading device and are respectively and correspondingly positioned right above the first fixing hole and the second fixing hole, and fixing needles are arranged in the guide holes and the corresponding first fixing holes and the guide holes and the corresponding second fixing holes in a penetrating manner;
the right side part of the spinal cord base is provided with a T-shaped mortise slot;
the nerve fixing component comprises a nerve base, the left side part of the nerve base is of a tenon structure matched with the mortise, more than two groups of nerve fixing sub-components are arranged on the nerve base, and the multiple groups of nerve fixing sub-components are arranged side by side on the left and right.
The left side of the spinal cord base is provided with a scale.
The height of the ridge is less than 1 mm.
Every nerve of group fixes sub-subassembly and includes dead lever and two at least ladder grooves, set up respectively in nerve base's nearly side and distal surface in two ladder grooves, the dead lever sets up the top at nerve base, the both ends of dead lever are the structure of buckling, and the both ends of dead lever are sliding connection respectively at two ladder inslots, the slot hole has been seted up along vertical direction at the both ends of dead lever, threaded hole has been seted up to every layer of ladder department top in ladder groove, the both ends slot hole interpolation of dead lever is equipped with positioning bolt, positioning bolt corresponds threaded connection in one of them threaded hole, positioning bolt's head and dead lever surface roof pressure cooperation.
The spinal cord base is provided with two groups of binding groove groups, the two groups of binding groove groups are symmetrically positioned on the left side and the right side of the ridge, each group of binding groove groups comprises at least one binding groove, and the binding grooves are parallel to the ridge.
The aperture of the first fixing hole and the aperture of the second fixing hole are both smaller than 1 mm.
Adopt above-mentioned technical scheme, the utility model discloses following beneficial effect has: the ridge corresponds to the anterior median fissure of the spinal cord, the needle threading device is used for guiding the fixing needle, the proximal end of the spinal cord can be fixed on the spinal cord base, the middle part of the spinal cord is fixed through the binding belt and the binding groove, and other parts of the spinal cord are allowed to move slightly, so that the resistance of isotropic contraction of the spinal cord in the tissue treatment stage is reduced to the maximum extent during the light transparent treatment; can fix nerve on nerve base through dead lever and ladder groove, mortise and tenon structure on spinal cord base and the nerve base forms mortise and tenon structure for the two both can the exclusive use, can jointly use again.
Drawings
FIG. 1 is a schematic structural view of a spinal fixation assembly;
fig. 2 is a schematic structural view of a nerve fixation subassembly.
Detailed Description
As shown in fig. 1 and fig. 2, the in vitro spinal cord and brachial plexus nerve fixator of the present invention comprises a spinal cord fixing component and a nerve fixing component, wherein the spinal cord fixing component comprises a spinal cord base 1, a raised ridge 2 is fixed in the middle of the spinal cord base 1, the height of the ridge 2 is smaller than 1mm, at least one first fixing hole is opened at the proximal end of the ridge 2, at least one second fixing hole is opened on the spinal cord base 1 and on the left and right sides of the first fixing hole, and the aperture of each of the first fixing hole and the second fixing hole is smaller than 1 mm;
a needle threading device 3 is fixed in the middle of the near side of the spinal cord base 1 through a support rod 14, the needle threading device 3 is positioned above the middle of the near side of the spinal cord base 1, a plurality of guide holes are formed in the needle threading device 3, the guide holes are respectively and correspondingly positioned right above the first fixing hole and the second fixing hole, and fixing needles 4 are arranged in the guide holes and the corresponding first fixing hole and second fixing hole in a penetrating manner;
the spinal cord base 1 is provided with two groups of binding grooves, the two groups of binding grooves are symmetrically positioned on the left side and the right side of the ridge 2, each group of binding grooves comprises at least one binding groove 5, and the binding grooves 5 are parallel to the ridge 2; a T-shaped mortise slot 6 is arranged at the right side part of the spinal cord base 1;
the nerve fixing component comprises a nerve base 7, the left side part of the nerve base 7 is of a tenon 15 structure matched with the mortise slot 6, more than two groups of nerve fixing sub-components are arranged on the nerve base 7, a plurality of groups of nerve fixing sub-components are arranged side by side on the left and right, each group of nerve fixing sub-component comprises a fixing rod 8 and two stepped slots 9, the two stepped slots 9 are arranged on the near side surface and the far side surface of the nerve base 7, the fixing rod 8 is arranged above the nerve base 7, the two end parts of the fixing rod 8 are of a bending structure, the two end parts of the fixing rod 8 are respectively connected in the two stepped slots 9 in a sliding manner, the two end parts of the fixing rod 8 are provided with long holes 10 along the vertical direction, a threaded hole 11 is arranged above each layer of the stepped slot 9, positioning bolts 12 are inserted into the long holes 10 at, the head of the positioning bolt 12 is in press fit with the outer surface of the fixed rod 8.
The left side of the spinal base 1 is provided with a scale 13, and the scale 13 is arranged to facilitate direct observation or measurement of changes in the length of the spinal cord.
The spinal cord fixing method comprises the following steps: placing an in-vitro spinal cord on a spinal cord base 1, enabling the anterior median fissure of the spinal cord to correspond to a ridge 2, guiding through a guide hole of a needle threader 3, fixing the proximal end of the spinal cord on the spinal cord base 1 by using 1-3 fixing needles 4, inserting the fixing needles 4 into the fixing holes, penetrating a binding groove 5 by using a binding band (such as an aluminum foil which is cut into a narrow strip shape), surrounding the widest part of the spinal cord, and fixing the binding band by using a small clip;
the nerve fixing method comprises the following steps: placing the isolated nerve on a nerve base 7, sliding the fixing rod 8 on the left side, fixing the near end of the nerve, screwing a positioning bolt 12 for positioning, sliding the fixing rod 8 on the right side, fixing the far end of the nerve, and positioning by using the positioning bolt 12;
spinal cord-nerve fixation method: connecting a tenon 15 structure of a nerve base 7 with a mortise 6 of a spinal cord base 1, placing an isolated spinal cord on the spinal cord base 1, enabling a front median fissure of the spinal cord to correspond to a ridge 2, fixing the proximal end of the spinal cord on the spinal cord base 1 by using 1-3 fixing needles 4 through the guidance of a guide hole of a needle threader 3, inserting the fixing needles 4 into the fixing holes, penetrating a binding groove 5 by using a binding band (such as an aluminum foil which is cut into a narrow strip shape), surrounding the widest part of the spinal cord, and fixing the binding band by using a small clip; the nerve in vitro is placed on the nerve base 7, the fixing rod 8 on the left side is slid, the near end of the nerve is fixed, the positioning bolt 12 is screwed to perform positioning, the fixing rod 8 on the right side is slid, the far end of the nerve is fixed, and positioning is performed by the positioning bolt 12.
The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any form, and all of the technical matters of the present invention belong to the protection scope of the present invention to any simple modification, equivalent change and modification made by the above embodiments.
Claims (6)
1. Mouse separation spinal cord and brachial plexus fixer, its characterized in that: comprises a spinal cord fixing component and a nerve fixing component; the spinal cord fixing component comprises a spinal cord base, wherein a raised ridge is fixed in the middle of the spinal cord base, at least one first fixing hole is formed in the near end of the ridge, and at least one second fixing hole is formed in the spinal cord base and positioned on the left side and the right side of the first fixing hole respectively;
a needle threading device is fixed in the middle of the near side of the spinal cord base through a support rod and is positioned above the middle of the near side of the spinal cord base, a plurality of guide holes are formed in the needle threading device and are respectively and correspondingly positioned right above the first fixing hole and the second fixing hole, and fixing needles are arranged in the guide holes and the corresponding first fixing holes and the guide holes and the corresponding second fixing holes in a penetrating manner;
the right side part of the spinal cord base is provided with a T-shaped mortise slot;
the nerve fixing component comprises a nerve base, the left side part of the nerve base is of a tenon structure matched with the mortise, more than two groups of nerve fixing sub-components are arranged on the nerve base, and the multiple groups of nerve fixing sub-components are arranged side by side on the left and right.
2. The isolated spinal cord and brachial plexus fixator for mouse of claim 1, wherein: the left side of the spinal cord base is provided with a scale.
3. The isolated spinal cord and brachial plexus fixator for mouse of claim 1, wherein: the height of the ridge is less than 1 mm.
4. The isolated spinal cord and brachial plexus fixator for mouse of claim 1, wherein: every nerve of group fixes sub-subassembly and includes dead lever and two at least ladder grooves, set up respectively in nerve base's nearly side and distal surface in two ladder grooves, the dead lever sets up the top at nerve base, the both ends of dead lever are the structure of buckling, and the both ends of dead lever are sliding connection respectively at two ladder inslots, the slot hole has been seted up along vertical direction at the both ends of dead lever, threaded hole has been seted up to every layer of ladder department top in ladder groove, the both ends slot hole interpolation of dead lever is equipped with positioning bolt, positioning bolt corresponds threaded connection in one of them threaded hole, positioning bolt's head and dead lever surface roof pressure cooperation.
5. The isolated spinal cord and brachial plexus fixator for mouse of claim 1, wherein: the spinal cord base is provided with two groups of binding groove groups, the two groups of binding groove groups are symmetrically positioned on the left side and the right side of the ridge, each group of binding groove groups comprises at least one binding groove, and the binding grooves are parallel to the ridge.
6. The isolated spinal cord and brachial plexus fixator for mouse of claim 1, wherein: the aperture of the first fixing hole and the aperture of the second fixing hole are both smaller than 1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921268223.0U CN210401186U (en) | 2019-08-07 | 2019-08-07 | Mouse in vitro spinal cord and brachial plexus fixator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921268223.0U CN210401186U (en) | 2019-08-07 | 2019-08-07 | Mouse in vitro spinal cord and brachial plexus fixator |
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| CN210401186U true CN210401186U (en) | 2020-04-24 |
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| CN201921268223.0U Active CN210401186U (en) | 2019-08-07 | 2019-08-07 | Mouse in vitro spinal cord and brachial plexus fixator |
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