CN210357213U - Centrifugal liquid releasing device - Google Patents
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- CN210357213U CN210357213U CN201920867092.1U CN201920867092U CN210357213U CN 210357213 U CN210357213 U CN 210357213U CN 201920867092 U CN201920867092 U CN 201920867092U CN 210357213 U CN210357213 U CN 210357213U
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Abstract
The utility model belongs to the technical field of micro-fluidic chip, a centrifugal liquid release device is disclosed, including centrifugal chip, centrifugal chip has the center of rotation, be provided with at least a set of release mechanism on the centrifugal chip, release mechanism includes liquid inlet, vesicle stop gear, extrusion mechanism, stock solution vesicle, liquid inlet with runner intercommunication in the centrifugal chip, vesicle stop gear is fixed to be set up the liquid inlet is kept away from one side of center of rotation, extrusion mechanism slides and sets up the liquid inlet is close to one side of center of rotation, the stock solution vesicle sets up vesicle stop gear with between the extrusion mechanism. The utility model discloses a centrifugal liquid release device can realize the release of liquid through simple means, and can realize the orderly release of multiunit liquid, and need not to adopt complicated mechanical structure.
Description
Technical Field
The utility model relates to a micro-fluidic chip technical field especially relates to a centrifugal liquid release.
Background
The centrifugal microfluidic chip is controlled by a single centrifuge to complete complex fluid reaction, is suitable for high-throughput detection, and is widely applied to the fields of biochemical analysis and in-vitro diagnosis. In order to improve the portability and controllability of the microfluidic chip detection product, it is generally required to pre-embed a required liquid reagent on the chip and to accomplish the controllable release of the reagent. The controlled sequential release of reagents helps to complete complex biochemical reactions on the chip. The prior centrifugal chip lacks a flexible, rapid and low-cost liquid release means. Moreover, the pre-embedding of the liquid reagent on the chip affects the stability of the chip, affects the activity of the pre-embedded freeze-drying reagent on the chip, and has high cost and complex operation. The storage and release of multiple reagents on a single chip often require the use of complex mechanical structures and even the addition of additional auxiliary equipment. Biochemical reactions typically involve multiple fluids and require multiple fluids to react in a sequence and at intervals, and thus conventional microfluidic chips involve multiple fluid control valves.
Although various proposals are made, such as chinese utility model patent with application number CN201410750822.1, disclosing a centrifugal-based microfluidic chip liquid reagent release method, the proposal can solve certain problems but still has the following defects: the liquid is stored in the bag, the sealing requirement of the bag is high, the relative position of the bag and the chip has a large influence on the release stability of the bag, in addition, the microfluidic chip is generally small in area, and the large area of the bag in the patent is not beneficial to the integration of other functional units of the chip.
As another example, the chinese utility model patent application with application number cn201410631614.x discloses an in vitro diagnostic test card, which can solve certain problems but still has the following defects: the liquid stored in the chip is released by squeezing, which, although controllable, increases the complexity of the device by adding moving parts.
Based on the above situation, there is a need to design a centrifugal liquid releasing device capable of solving the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: a centrifugal liquid discharge device is provided which can discharge liquid by a simple means and can discharge a plurality of groups of liquids in order without using a complicated mechanical structure.
To achieve the purpose, the utility model adopts the following technical proposal:
a centrifugal liquid release device comprises a centrifugal chip, wherein the centrifugal chip is provided with a rotation center, at least one set of release mechanisms is arranged on the centrifugal chip, each release mechanism comprises a liquid inlet, a vesicle limiting mechanism, an extrusion mechanism and liquid storage vesicles, the liquid inlet is communicated with a flow channel in the centrifugal chip, the vesicle limiting mechanism is fixedly arranged on one side, away from the rotation center, of the liquid inlet, the extrusion mechanism is arranged on one side, close to the rotation center, of the liquid inlet in a sliding mode, and the liquid storage vesicles are arranged between the vesicle limiting mechanism and the extrusion mechanism.
Specifically, the centrifugal control device drives the centrifugal chip to rotate around the rotation center, the extrusion mechanism slides towards the vesicle limiting mechanism under the action of centrifugal force, the extrusion mechanism extrudes the liquid storage vesicle pre-packaged with target liquid onto the vesicle limiting mechanism, at the moment, the liquid storage vesicle is positioned above the liquid inlet, and when the centrifugal force is large enough, the liquid storage vesicle is broken, and liquid in the liquid storage vesicle is released and enters the centrifugal chip through the liquid inlet.
Preferably, the centrifugal chip is disc-shaped, the rotation center is the center of the disc, and the extrusion mechanism, the liquid storage vesicle and the vesicle limiting mechanism are sequentially arranged outwards along the radial direction of the centrifugal chip.
Preferably, the shapes of the vesicle limiting mechanism, the extrusion mechanism and the liquid storage vesicle are matched, so that the liquid storage vesicle and the extrusion mechanism are prevented from obviously displacing under high-speed rotation, the liquid storage vesicle can be completely extruded, and the liquid encapsulated in the liquid storage vesicle can be completely released.
Preferably, the pressing mechanism is a pressing block.
Preferably, the centrifugal control device is a centrifuge, and the centrifuge is fixedly connected with the rotation center.
As a preferable technical solution, the vesicle limiting mechanism is provided with a puncture structure facing the liquid storage vesicle.
In particular, the piercing structure has a sharp tip, and when the reservoir vesicle is squeezed onto the vesicle limiting mechanism, the piercing structure can pierce the reservoir vesicle more easily. The number of the puncture structures is one or more.
As a preferred solution, the piercing structure is near the bottom of the reservoir vesicle.
Specifically, the puncturing structure is close to the bottom of the liquid storage vesicle, so that the bottom of the liquid storage vesicle is preferentially punctured when the liquid storage vesicle is broken, and the liquid in the liquid storage vesicle is completely released.
As a preferable technical solution, a fixing groove is provided on the centrifugal chip, and the lower end of the vesicle limiting mechanism is detachably provided on the fixing groove.
Specifically, vesicle stop gear lower extreme be provided with fixed slot matched with grafting portion, grafting portion shape and size with fixed slot shape and size cooperatees, grafting portion with the fixed slot forms the plug cooperation. The vesicle limiting mechanism is detachably connected with the centrifugal chip, so that the vesicle limiting mechanism is convenient to separately store, package and transport, and can be assembled in use, and the flexibility of the device is improved.
As a preferable technical solution, the lower end of the vesicle limiting mechanism covers a part of the liquid inlet.
Specifically, the vesicle limiting mechanism is positioned on one side of the liquid inlet far away from the rotation center, and a part of the opening of the liquid inlet far away from the rotation center is covered by the lower end of the vesicle limiting mechanism, so that liquid flowing down along the vesicle limiting mechanism can directly flow into the liquid inlet, the liquid is prevented from entering a fixed groove to cause waste, and the released liquid is ensured to completely enter the centrifugal chip through the liquid inlet.
As a preferable technical solution, a U-shaped opening is provided at one side of the vesicle limiting mechanism facing the liquid storage vesicle, and the shape of the liquid storage vesicle and/or the extrusion mechanism is matched with the U-shaped opening.
Specifically, the liquid storage vesicle and the extrusion mechanism can be accommodated in the U-shaped opening, and the U-shaped opening can limit the liquid storage vesicle and the extrusion mechanism, so that the liquid storage vesicle and the extrusion mechanism slide along the vesicle limiting mechanism, and the liquid storage vesicle and the extrusion mechanism are ensured not to be displaced obviously when the centrifugal chip rotates at a high speed. And the liquid storage vesicle can be completely extruded, and the liquid encapsulated in the liquid storage vesicle can be completely released.
Preferably, the shapes of the liquid storage vesicle and the extrusion mechanism are matched with the U-shaped opening.
As a preferred technical scheme, a limiting plate is arranged at the upper end of the vesicle limiting mechanism.
Specifically, the limiting plate is arranged at the upper end of the U-shaped vesicle limiting mechanism, so that the liquid storage vesicle and the extrusion block cannot deviate from the whole vesicle limiting mechanism under the centrifugal action.
As a preferable technical solution, a hydrophobic layer is disposed on one side of the vesicle limiting mechanism facing the liquid storage vesicle.
Specifically, the inner wall of one side of the vesicle limiting mechanism, which faces the liquid storage vesicle, is subjected to hydrophobic treatment to form a hydrophobic layer, so that the adsorption of the vesicle limiting mechanism on a liquid reagent can be reduced, and liquid can flow down smoothly.
As a preferable technical solution, an inner wall of the vesicle limiting mechanism on a side facing the liquid storage vesicle is an inclined structure, and an upper end of the inclined structure is closer to the rotation center than a lower end thereof.
In particular, the inner wall has an inclined angle, and liquid can more easily enter the liquid inlet under the action of centrifugal force.
As a preferable technical solution, a magnetic mechanism is provided on the vesicle limiting mechanism and/or the squeezing mechanism.
Specifically, magnets which attract each other are respectively arranged on the vesicle limiting mechanism and the extrusion mechanism, or one of the vesicle limiting mechanism and the extrusion mechanism is provided with a magnet, and the other is provided with a structure made of a material which can be adsorbed by the magnet. Therefore, the fixing of the extrusion mechanism and the vesicle limiting mechanism, the extrusion of the liquid storage vesicle and the release of liquid are facilitated.
The utility model has the advantages that: the centrifugal liquid release device is provided, the release of liquid is realized by a simple means, and the ordered release of a plurality of groups of liquid can be realized without adopting a complex mechanical structure;
the liquid storage vesicle does not need to be pre-embedded on the chip in advance, so that the space of the chip is saved, and the storage and the ordered release of various reagents on a single chip are facilitated;
avoid the direct storage of liquid on the chip, strengthen the stability of chip, especially to having the freeze-drying reagent on the chip under, the stock solution vesicle independently exists, avoids the interference to freeze-drying reagent.
The reagent is separated from the chip, the type and volume of the reagent can be changed at will, and the flexibility is good;
the liquid storage vesicle and the vesicle limiting mechanism on the centrifugal chip are extruded by the extruding mechanism under the action of centrifugal force, so that the liquid storage vesicle is crushed, and liquid is released;
the liquid storage vesicle and the extrusion mechanism are assembled, so that the flexibility is good, the use of a complex mechanical structure is avoided, and the use of a complex fluid control valve is avoided;
the extrusion mechanism and the vesicle limiting mechanism can be repeatedly used, and the cost is low.
Drawings
Fig. 1 is a schematic structural diagram of a first embodiment of the present invention;
fig. 2 is a schematic diagram of a first embodiment of the present invention in an in-use state;
fig. 3 is a schematic diagram of a state after use according to the first embodiment of the present invention;
fig. 4 is a schematic structural view of a bubble limiting mechanism and a fixing groove according to an embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view of a bladder limiting mechanism and an extrusion mechanism according to an embodiment of the present invention;
fig. 6 is a schematic cross-sectional view of another shape of the vesicle limiting mechanism and the extrusion mechanism according to the first embodiment of the present invention;
FIG. 7 is a schematic structural view illustrating an inner wall of a bubble-limiting machine according to an embodiment of the present invention in an inclined structure;
fig. 8 is a schematic structural diagram of a second embodiment of the present invention.
In fig. 1 to 7, a centrifugal chip 1, a rotation center 2, a liquid inlet 3, a vesicle limiting mechanism 4, a squeezing mechanism 5, a liquid storage vesicle 6, a puncture structure 7, a fixing groove 8, a U-shaped opening 9, a limiting plate 10, and a plugging portion 41 are shown.
In FIG. 8, the release mechanism (100,200,300,400), the vesicle restriction mechanism (104,204,304, 404).
Detailed Description
In order to further understand and appreciate the structural features and advantages of the present invention, preferred embodiments and the accompanying drawings are described in detail as follows:
example one
A centrifugal liquid release device is shown in figure 1 and comprises a centrifugal chip 1, wherein the centrifugal chip 1 is provided with a rotation center 2, at least one set of release mechanisms (only one set of release mechanisms is shown in figures 1 to 4 and 7 for convenience of description) is arranged on the centrifugal chip 1, each release mechanism comprises a liquid inlet 3, a vesicle limiting mechanism 4, a squeezing mechanism 5 and a liquid storage vesicle 6, the liquid inlet 3 is communicated with a flow channel in the centrifugal chip 1, the vesicle limiting mechanism 4 is fixedly arranged on one side, away from the rotation center 2, of the liquid inlet 3, the squeezing mechanism 5 is slidably arranged on one side, close to the rotation center 2, of the liquid inlet 3, and the liquid storage vesicle 6 is arranged between the vesicle limiting mechanism 4 and the squeezing mechanism 5.
The centrifugal chip 1 is in a disc shape, the rotation center 2 is a circle center of the disc, a centrifuge (not shown) is fixedly connected with the rotation center 2 and can drive the centrifugal chip 1 to rotate, and the rotation speed, acceleration, deceleration and rotation direction of the centrifugal chip 1 can be controlled through a certain control program. The extrusion mechanism 5, the liquid storage vesicle 6 and the vesicle limiting mechanism 4 are sequentially arranged outwards along the radial direction of the centrifugal chip 1, when the rotating speed of the centrifugal chip 1 is large enough and the centrifugal force is large enough, the liquid storage vesicle 6 can be extruded and broken between the extrusion mechanism 5 and the vesicle limiting mechanism 4, and the liquid in the liquid storage vesicle 6 is released and enters a micro-channel in the centrifugal chip 1 through the liquid inlet 3.
The material of the centrifugal chip 1 includes glass, silicon wafer or common polymer material. The polymer material includes Polydimethylsiloxane (PDMS), polyurethane, epoxy resin, polymethyl methacrylate (PMMA), Polycarbonate (PC), Cyclic Olefin Copolymer (COC), Polystyrene (PS), Polyethylene (PE) and fluoroplastic. The material of the centrifugal chip 1 may be one or more of the above materials. The processing method of the centrifugal chip 1 can select one or more of different methods such as photoetching, numerical control, pouring, injection molding, laser engraving, plasma etching, wet etching and the like according to the material and the structure.
The liquid storage vesicle 6 contains the target liquid, and the volume of the target liquid is set according to the experimental requirements (the volume is 2-200 μ L which is common in the field), and the material can be soft polymer or composite material which is common in the field, such as aluminum composite material. The processing of the reservoir vesicle 6 is conventional in the art and will not be described further herein. The liquid storage vesicle 6 needs to be placed between the vesicle limiting mechanism 4 and the extrusion mechanism 5, namely, the shape of the liquid storage vesicle 6 can be set according to the processing conditions and the shapes of the vesicle limiting mechanism 4 and the extrusion mechanism 5.
The material of the vesicle limiting mechanism 4 can be common materials such as polymer, metal, glass and the like. The processing method can be common means such as numerical control processing, laser processing or injection molding processing. The extrusion mechanism 5 is an extrusion block, and the extrusion block is a solid (metal, polymer, glass, etc.) made of hard materials.
In this embodiment, as shown in fig. 1, the vesicle restriction mechanism 4 is provided with a puncture structure 7 facing the liquid storage vesicle 6. As shown in fig. 2 to 3, the puncturing structure 7 has a sharp tip, and when the liquid storage vesicle 6 is pressed onto the vesicle limiting mechanism 4, the puncturing structure 7 can more easily puncture the liquid storage vesicle 6, so as to release the liquid in the liquid storage vesicle 6. The number of the puncture structures 7 is one or more. If needed, the polymer material on one side of the liquid storage vesicle 6 can be thinned in the processing process of the liquid storage vesicle 6, and one side of the thin liquid storage vesicle 6 is contacted with the puncture structure 7 during installation, so that the liquid in the liquid storage vesicle 6 can be released more favorably.
In this embodiment, as shown in fig. 1, the puncturing structure 7 is close to the bottom of the liquid storage vesicle 6, so that the bottom of the liquid storage vesicle 6 is preferentially punctured when the liquid storage vesicle 6 is ruptured, which is beneficial to completely releasing the liquid in the liquid storage vesicle 6. In other embodiments, the puncture structure 7 may be located at any position on the inner wall of the vesicle limiting mechanism 4, and it is only necessary to ensure that the squeezing mechanism 5 squeezes the liquid storage vesicle 6 to contact with the puncture structure 7 under the action of centrifugal force.
In this embodiment, as shown in fig. 4, the centrifugal chip 1 is provided with a fixing groove 8, and the lower end of the vesicle limiting mechanism 4 is detachably disposed on the fixing groove 8. The lower end of the vesicle limiting mechanism 4 is provided with a plugging part 41 matched with the fixing groove 8, the shape and the size of the plugging part 41 are matched with the shape and the size of the fixing groove 8, the plugging part 41 and the fixing groove 8 form plugging and unplugging cooperation, and when the centrifugal chip 1 rotates, the vesicle limiting mechanism 4 is tightly inserted into the fixing groove 8 through the plugging part 41.
In this embodiment, the lower end of the vesicle restriction mechanism 4 covers a portion of the liquid inlet 3. As shown in fig. 4, the width of the plug portion 41 is W1, the width of the fixing groove 8 is W2, W1> W2, and after the vesicle restriction mechanism 4 is inserted into the fixing groove 8, it is ensured that the lower end of the vesicle restriction mechanism 4 covers only a part of the liquid inlet 3 but does not cover the entire liquid inlet 3, and the entire released liquid enters the centrifugal chip 1 through the liquid inlet 3 without remaining in the fixing groove 8.
In this embodiment, as shown in fig. 5 to 6, a U-shaped opening 9 is provided on a side of the vesicle limiting mechanism 4 facing the liquid storage vesicle 6, and the shapes of the liquid storage vesicle 6 and the squeezing mechanism 5 are matched with the U-shaped opening 9. The U-shaped opening shown in fig. 5 is an arc-shaped opening, the extrusion end of the extrusion mechanism 5 is arc-shaped, the U-shaped opening shown in fig. 6 is a frame-shaped opening, and the extrusion end of the extrusion mechanism 5 is frame-shaped. The U-shaped opening 9 can accommodate the liquid storage vesicle 6 and the extrusion mechanism 5, the U-shaped opening 9 can limit the liquid storage vesicle 6 and the extrusion mechanism 5, and the liquid storage vesicle 6 and the extrusion mechanism 5 slide along the vesicle limiting mechanism 4 without obvious displacement when the centrifugal chip 1 rotates at a high speed. And ensures that the reservoir vesicle 6 can be fully squeezed to completely release the encapsulated liquid. It should be noted that the U-shaped opening is not limited to the illustrated form, as long as it is ensured that the liquid storage bladder 6 and the squeezing mechanism 5 do not displace significantly when the centrifugal chip 1 rotates at a high speed.
In this embodiment, as shown in fig. 1, a limiting plate 10 is disposed at the upper end of the vesicle limiting mechanism 4, and the limiting plate 10 is fixedly disposed at the upper end of the limiting mechanism 4, so as to prevent the liquid storage vesicle 6 and the extrusion mechanism 5 from sliding out from the upper side of the vesicle limiting mechanism 4 when the centrifugal chip 1 rotates at a high speed. In this embodiment, the limiting plate 10 and the vesicle limiting mechanism 4 are integrally formed.
In this embodiment, the inner wall of the vesicle limiting mechanism 4 facing the side of the liquid storage vesicle 6 is provided with a hydrophobic layer.
In this embodiment, as shown in fig. 7, the inner wall of the vesicle restriction mechanism 4 facing the side of the liquid storage vesicle 6 is an inclined structure, the upper end of the inclined structure is closer to the rotation center 2 than the lower end, and at this time, the shape of the side of the extrusion mechanism facing the liquid storage vesicle 6 is also an inclined structure.
In this embodiment, the vesicle limiting mechanism 4 and/or the squeezing mechanism 5 are provided with magnetic mechanisms. Magnets which attract each other are respectively arranged on the vesicle limiting mechanism 4 and the extrusion mechanism 5, or one of the vesicle limiting mechanism 4 and the extrusion mechanism 5 is provided with a magnet, and the other is provided with a structure made of a material which can be adsorbed by the magnet. Thus being more beneficial to the fixation of the extrusion mechanism 5 and the vesicle limiting mechanism 4, the extrusion of the liquid storage vesicle 6 and the release of liquid.
When biochemical reaction involves multiple fluids, and needs the reaction of multiple fluids according to certain order and time interval, can be in the utility model discloses in set up a plurality of fluid entry 3 on centrifugal chip 1, corresponding a plurality of vesicle stop gear 4, extrusion mechanism 5, stock solution vesicle 6 that set up.
The utility model discloses can realize fluidic orderly release through a plurality of factors, the main factor has included:
the distance between the release mechanism and the rotation center 2 is longer and earlier when the release mechanism is farther from the rotation center 2 under the same condition;
the weight of the extrusion mechanism 5 is equal to that of the extrusion mechanism 5, and the heavier the extrusion mechanism 5 is, the earlier the extrusion mechanism is released;
other important factors include the material of the liquid storage vesicle 6 (the type and thickness of the packaging material), the shape of the squeezing mechanism 5, and the like.
Ordered release of multiple sets of liquids can be achieved by adjusting the above factors.
Example two
The present embodiment takes magnetic particle double-antibody sandwich chemiluminescence as an example, and details the working process of the centrifugal liquid release device of the present invention.
Magnetic particle double-antibody sandwich chemiluminescence is widely applied to clinical monitoring as a common reaction system in the field of in vitro diagnosis at present. The centrifugal chip is widely applied to basic level inspection mechanisms and the like due to the characteristics of small equipment, simplicity in operation and control and the like.
In order to improve the stability of the reagent and reduce the low temperature requirement during transportation, the lyophilized form of antibody and the like is usually pre-stored in the centrifugal chip in the field at present. The reconstitution of the lyophilized antibody requires the addition of a diluent, which is likely to affect the activity of the lyophilized antibody if pre-embedded on a centrifugal chip, for example, the above diluent makes the lyophilized antibody wet by volatilization.
In this embodiment, taking magnetic particle double-antibody sandwich chemiluminescence as an example, as shown in fig. 8, antibodies, magnetic beads, and the like required for the reaction are pre-embedded in the centrifugal chip 1 by freeze-drying. This embodiment provides four sets of release mechanisms (100,200,300,400) on the centrifugal chip, each set of release mechanisms showing only the corresponding vesicle limiting mechanism (104,204,304,404) for ease of illustration, and the fluid inlet, squeezing mechanism, and reservoir vesicles not shown. The rest of the structure is the same as that in the first embodiment, and is not described again here.
The liquids encapsulated in the liquid storage vesicle (not shown) at the four groups of release mechanisms (100,200,300 and 400) are respectively cleaning liquid, substrate liquid, primary anti-diluent and magnetic bead diluent.
The design of the chip flow channel is designed according to the reaction requirement, which is not the key point of the present invention, and is a conventional technical means in the field, and therefore, is not shown in the figure.
In practice, four sets of release mechanisms (100,200,300,400) are first assembled on the centrifugal chip 1. The release mechanism (100) and the release mechanism (200) are on the same circumference, and the release mechanism (300) and the release mechanism (400) are on the same circumference, with the center of the disk-shaped centrifugal chip 1 as the center of a circle. Wherein the mass of the squeezing mechanism (not shown) at the release mechanism (300) is greater than the mass of the squeezing mechanism (not shown) at the release mechanism (400), and the material of the reservoir vesicle (not shown) at the release mechanism (100) is thinner than the reservoir vesicle (not shown) at the release mechanism (200).
During the reaction process, the substance to be measured is firstly added into the target flow channel of the centrifugal chip 1.
Because the release mechanism (300) and the release mechanism (400) are far away from the center of a circle, compared with the release mechanism (100) and the release mechanism (200), under the condition that the rotating speed is gradually increased, liquid in the liquid storage vesicle (not shown) at the positions of the release mechanism (300) and the release mechanism (400) is firstly released, because the mass of the extrusion mechanism (not shown) at the position of the release mechanism (300) is larger than that of the extrusion mechanism (not shown) at the position of the release mechanism (400), at the rotating speed of omega 1, the anti-diluent at the position of the release mechanism (300) is firstly released, and the anti-diluent is combined with the substance to be detected after.
When the rotating speed is increased to omega 2, the magnetic bead diluent at the releasing mechanism (400) is released, the magnetic beads are redissolved and participate in the reaction to form a double-antibody sandwich structure.
When the rotating speed is increased to omega 3, the releasing mechanism (100) and the releasing mechanism (200) are close to the center of a circle, liquid in the liquid storage vesicle (not shown) at the releasing mechanism (100) and the releasing mechanism (200) starts to be released, and as the material of the liquid storage vesicle at the releasing mechanism (100) is thinner than that of the liquid storage vesicle at the releasing mechanism (200), the cleaning liquid at the releasing mechanism (100) is released firstly, and the formed double-antibody sandwich structure is cleaned.
When the rotating speed is increased to omega 4, the substrate liquid at the position of the releasing mechanism (200) is released to react with the double-antibody sandwich structure, and the luminescence is detected to complete the magnetic particle double-antibody sandwich chemiluminescence reaction.
The above ω 1> ω 2> ω 3> ω 4.
The ordered release of the liquid can be realized by adjusting the position of the release mechanism, the material and thickness of the liquid storage vesicle, the centrifugal rotation speed, the shape and quality of the extrusion mechanism, the number and arrangement of the release mechanisms and other factors, which are not described herein again.
The parts not related to in the utility model are all the same with the prior art or can be realized by adopting the prior art.
Finally, it should be noted that: in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "front", "rear", "inner", "outer", "vertical", "horizontal", etc. indicate that the directions or positional relationships are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and understanding of the technical solutions of the present invention, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. A centrifugal liquid release device comprising a centrifugal chip (1), the centrifugal chip (1) having a centre of rotation (2), characterized in that the centrifugal chip (1) is provided with at least one set of release mechanisms, the release mechanism comprises a liquid inlet (3), a vesicle limiting mechanism (4), an extrusion mechanism (5) and a liquid storage vesicle (6), the liquid inlet (3) is communicated with a flow channel in the centrifugal chip (1), the vesicle limiting mechanism (4) is fixedly arranged on one side of the liquid inlet (3) far away from the rotation center (2), the squeezing mechanism (5) is arranged on one side of the liquid inlet (3) close to the rotating center (2) in a sliding way, the liquid storage vesicle (6) is arranged between the vesicle limiting mechanism (4) and the extrusion mechanism (5).
2. A centrifugal liquid release device according to claim 1, wherein the vesicle limiting means (4) is provided with a puncture structure (7) facing the reservoir vesicle (6).
3. A centrifugal liquid release device according to claim 2, wherein the puncture structure (7) is near the bottom of the reservoir vesicle (6).
4. A centrifugal liquid discharge apparatus according to claim 1, wherein the centrifugal chip (1) is provided with a fixing groove (8), and the lower end of the vesicle limiting mechanism (4) is detachably provided on the fixing groove (8).
5. A centrifugal liquid release device according to claim 4, wherein the lower end of the vesicle limiting means (4) covers a part of the liquid inlet (3).
6. A centrifugal liquid release device according to claim 1, wherein the side of the vesicle limiting means (4) facing the reservoir vesicle (6) is provided with a U-shaped opening (9), and the reservoir vesicle (6) and/or the squeezing means (5) are shaped to fit the U-shaped opening (9).
7. A centrifugal liquid release device according to claim 6, characterized in that the upper end of the vesicle limiting mechanism (4) is provided with a limiting plate (10).
8. A centrifugal liquid release device according to claim 1, wherein the inner wall of the side of the vesicle limiting means (4) facing the reservoir vesicle (6) is provided with a hydrophobic layer.
9. A centrifugal liquid discharge apparatus according to claim 1, wherein the inner wall of the vesicle limiting means (4) on the side facing the reservoir vesicle (6) is of an inclined structure, the upper end of which is closer to the rotation center (2) than the lower end.
10. A centrifugal liquid release device according to claim 1, wherein magnetic means are provided on the vesicle limiting means (4) and/or the squeezing means (5).
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| CN201920867092.1U CN210357213U (en) | 2019-06-10 | 2019-06-10 | Centrifugal liquid releasing device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302850A (en) * | 2019-06-10 | 2019-10-08 | 东莞东阳光医疗智能器件研发有限公司 | A kind of centralized liquid release device |
| CN114405566A (en) * | 2022-02-08 | 2022-04-29 | 江苏液滴逻辑生物技术有限公司 | Freeze-drying ball pre-embedded structure, digital micro-fluidic chip and pre-embedded liquid injection method |
-
2019
- 2019-06-10 CN CN201920867092.1U patent/CN210357213U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302850A (en) * | 2019-06-10 | 2019-10-08 | 东莞东阳光医疗智能器件研发有限公司 | A kind of centralized liquid release device |
| CN114405566A (en) * | 2022-02-08 | 2022-04-29 | 江苏液滴逻辑生物技术有限公司 | Freeze-drying ball pre-embedded structure, digital micro-fluidic chip and pre-embedded liquid injection method |
| CN114405566B (en) * | 2022-02-08 | 2022-12-02 | 江苏液滴逻辑生物技术有限公司 | Freeze-drying ball pre-embedded structure, digital micro-fluidic chip and pre-embedded liquid injection method |
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Denomination of utility model: A centrifugal liquid release device Effective date of registration: 20220728 Granted publication date: 20200421 Pledgee: China Development Bank Shenzhen Branch Pledgor: Dongguan Dongyang Sunshine Medical Intelligent Device R&D Co.,Ltd. Registration number: Y2022980011450 |