CN210347670U - Dengue fever colloidal gold detection card - Google Patents
Dengue fever colloidal gold detection card Download PDFInfo
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- CN210347670U CN210347670U CN201920617138.4U CN201920617138U CN210347670U CN 210347670 U CN210347670 U CN 210347670U CN 201920617138 U CN201920617138 U CN 201920617138U CN 210347670 U CN210347670 U CN 210347670U
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- cover plate
- test paper
- colloidal gold
- pad
- dengue
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model relates to the technical field of immunodetection, and discloses a dengue colloidal gold detection card, which comprises a shell and detection test paper, wherein the shell is formed by buckling an upper cover plate and a lower cover plate, a sealing gasket is arranged at the joint of the lower cover plate and the upper cover plate, a window and a water injection hole are arranged on the upper cover plate, and a test paper groove is arranged in the lower cover plate; the test paper groove is provided with a sliding cover, the sliding cover is connected with the test paper groove in a sliding mode, the sliding cover is provided with an observation window, the observation window is arranged corresponding to the window, and a sealing film is arranged on the observation window; the detection test paper is placed in the test paper groove and comprises a PVC lining plate, a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and a water absorption pad, wherein the colloidal gold combined pad is coated with an anti-dengue virus monoclonal antibody 1; the lower cover plate is also internally provided with a self-heating bag which is provided with a pull buckle. The utility model discloses can be used for heating this detection card after detecting the completion to in time with the dengue virus inactivation, so that follow-up processing reduces the possibility of infection, further ensures operating personnel's self safety.
Description
Technical Field
The utility model relates to an immunoassay technical field has especially related to a dengue fever colloidal gold detects card.
Background
Dengue is an acute insect-borne infectious disease caused by dengue virus transmission through mosquito vectors. The dengue virus is a flavivirus genus in the Flaviviridae family, is a single-strand positive-strand RNA virus, and can cause recessive infection, dengue fever and dengue hemorrhagic fever after dengue virus infection, wherein the dengue hemorrhagic fever is rare in China. Typical clinical manifestations of dengue fever are sudden onset of illness, high fever, headache, severe soreness of muscles and joints, rash in some patients, bleeding tendency, swollen lymph nodes, decreased white blood cell count, thrombocytopenia, etc. The dengue virus is low temperature resistant, and can survive for 5 years at-20 ℃ and can survive for more than 8 years at-70 ℃ in human serum. However, dengue virus is not heat-stable and can be inactivated at 50 ℃ for 30 minutes or at 100 ℃ for 2 minutes.
At present, the utility model patent of patent application number 201721578046.7 discloses a dengue fever detector, including the detection card that is the setting of cuboid form, be equipped with detection zone and display area in the one side of detection card, be on a parallel with detection zone place plane in the detection card and be equipped with the sliding tray, the sliding tray is located the detection zone both sides, slides in the sliding tray and is equipped with the slide board, and the slide board can cover the opening of detection zone. This application will slide the slide plate to detection zone department, can utilize the slide plate to block the opening of detection zone to seal the detection zone, avoid when transporting or handling the detection card, blood sample flows from the detection zone. However, the dengue virus is not inactivated, so that the defect of easy infection exists.
SUMMERY OF THE UTILITY MODEL
The utility model provides an above-mentioned shortcoming to exist among the prior art provides a dengue fever colloidal gold detects card.
In order to solve the technical problem, the utility model discloses a following technical scheme can solve:
the dengue colloidal gold detection card comprises a shell and detection test paper, wherein the shell is formed by buckling an upper cover plate and a lower cover plate, a sealing gasket is arranged at the joint of the lower cover plate and the upper cover plate, a window and a water injection hole are arranged on the upper cover plate, and a test paper groove is arranged in the lower cover plate; the test paper groove is provided with a sliding cover, the sliding cover is connected with the test paper groove in a sliding mode, the sliding cover is provided with an observation window, the observation window is arranged corresponding to the window, and a sealing film is arranged on the observation window; the detection test paper is placed in the test paper groove and comprises a PVC lining plate, a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the water absorption pad are sequentially and partially overlapped and laid on the PVC lining plate, the colloidal gold combined pad is coated with a dengue virus resistant monoclonal antibody 1, an observation window and the nitrocellulose membrane are correspondingly arranged, the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is arranged on one side close to the colloidal gold combined pad, and the quality control line is arranged on one side close to the water absorption pad; the lower cover plate is also internally provided with a self-heating bag which is provided with a pull buckle.
Preferably, a support column is arranged in the lower cover plate, and the test paper groove is fixed on the support column.
Preferably, the sample pad is a glass fiber membrane, a polyester membrane, or filter paper fibers.
Preferably, the window is provided with a sealing film, and the water injection hole is provided with a sealing cover.
Preferably, the detection line is coated with an anti-dengue virus monoclonal antibody 2, and the quality control line is coated with a goat anti-mouse IgG antibody.
Wherein, the anti-dengue virus monoclonal antibody 1 and the anti-dengue virus monoclonal antibody 2 are monoclonal antibodies of different epitopes of dengue virus antigens.
The detection principle is as follows:
the dengue fever colloidal gold detection card of the utility model can be used for qualitatively detecting dengue virus antigens in human serum/plasma/whole blood samples. If the sample contains dengue virus antigen, firstly reacting with the anti-dengue virus monoclonal antibody 1 on the colloidal gold conjugate pad to form a compound, then under the action of chromatography, capturing the compound by the anti-dengue virus monoclonal antibody 2 coated on the detection line and the goat anti-mouse IgG coated on the quality control line in sequence, and forming two macroscopic red reaction lines on the detection line and the quality control line respectively; otherwise, only one red reaction line appears on the quality control line.
The utility model discloses owing to adopted above technical scheme, have apparent technological effect:
the dengue fever colloidal gold detection card of the utility model is provided with a sealing gasket at the joint of the lower cover plate and the upper cover plate, a sealing cover is arranged on the water injection hole of the shell, and a sealing film is arranged on the window, so that the sealing performance of the shell can be increased, and the sample is prevented from leaking to cause infection; the sliding cover is arranged on the test paper slot, so that the test paper slot is convenient to open during sample adding, and can also be closed in time after the sample adding is finished, and a sample is isolated; the built-in self-heating bag can tear the pull button after detection is finished, and clean water is injected for heating the detection card, so that the dengue virus can be inactivated in time, subsequent treatment is facilitated, the possibility of infection is reduced, and the safety of operators is further guaranteed. In addition, fixed with the support column for the test paper groove, during the heating water injection, rivers flow to the test paper groove bottom, are favorable to the even heating to inactivate the dengue virus better.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a top view of the lower cover plate.
FIG. 3 is a schematic view of the structure of the test strip.
The names of the parts indicated by the numerical references in the drawings are as follows: 1-shell, 11-upper cover plate, 111-window, 112-water injection hole, 12-lower cover plate, 13-sealing gasket, 2-test paper groove, 21-sliding cover, 211-observation window, 3-test paper, 31-sample pad, 32-colloidal gold combined pad, 33-nitrocellulose membrane, 331-test line, 332-quality control line, 34-water absorption pad, 35-PVC lining plate, 4-support column, 5-self-heating bag, 51-pulling buckle.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example 1
A dengue colloidal gold detection card is shown in figures 1-3 and comprises a shell 1 and detection test paper 3, wherein the shell 1 is formed by buckling an upper cover plate 11 and a lower cover plate 12, a sealing gasket 13 is arranged at the joint of the lower cover plate 12 and the upper cover plate 11, a window 111 and a water injection hole 112 are arranged on the upper cover plate 11, and a test paper groove 2 is arranged in the lower cover plate 12; a sliding cover 21 is arranged on the test paper groove 2, the sliding cover 21 is connected with the test paper groove 2 in a sliding manner, an observation window 211 is arranged on the sliding cover 21, the observation window 211 is arranged corresponding to the window 111, and a sealing film is arranged on the observation window 211; the detection test paper 3 is placed in the test paper groove 2, the detection test paper 3 comprises a PVC lining plate 35, a sample pad 31, a colloidal gold combined pad 32, a nitrocellulose membrane 33 and a water absorption pad 34, the sample pad 31, the colloidal gold combined pad 32, the nitrocellulose membrane 33 and the water absorption pad 34 are sequentially and partially overlapped and laid on the PVC lining plate 35, the colloidal gold combined pad 32 is coated with a dengue virus resistant monoclonal antibody 1, an observation window 211 is arranged corresponding to the nitrocellulose membrane 33, the nitrocellulose membrane 33 is provided with a detection line 331 and a quality control line 332, the detection line 331 is arranged on one side close to the colloidal gold combined pad 32, and the quality control line 332 is arranged on one side close to the water absorption pad 34; the lower cover plate 12 is also provided with a self-heating bag 5, and the self-heating bag 5 is provided with a pull buckle 51.
A support column 4 is arranged in the lower cover plate 12, and the test paper groove 2 is fixed on the support column 4.
The sample pad 31 is a fiberglass membrane.
The window 111 is provided with a sealing film, and the water injection hole 112 is provided with a sealing cover.
The detection line 331 is coated with an anti-dengue virus monoclonal antibody 2, and the quality control line 332 is coated with a goat anti-mouse IgG antibody.
Wherein, the anti-dengue virus monoclonal antibody 1 and the anti-dengue virus monoclonal antibody 2 are monoclonal antibodies of different epitopes of dengue virus antigens.
And (3) judging a detection result:
positive: the quality control line shows a red color band, and the detection line also shows a red reaction line;
negative: the quality control line shows a red reaction line, the detection line does not show a red reaction line, and the test line is judged to be negative;
and (4) invalidation: the quality control line does not display a red reaction line, and the detection test paper is judged to be invalid no matter whether the detection line displays the red reaction line or not.
Example 2
The same as example 1, except that the sample pad 31 is a filter paper fiber in this example.
Example 3
The same as example 1, except that the sample pad 31 is a polyester film.
In short, the above description is only a preferred embodiment of the present invention, and all the equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the scope of the present invention.
Claims (5)
1. Dengue fever colloidal gold detects card, including casing (1) and test paper (3), its characterized in that: the shell (1) is formed by buckling an upper cover plate (11) and a lower cover plate (12), a sealing gasket (13) is arranged at the joint of the lower cover plate (12) and the upper cover plate (11), a window (111) and a water injection hole (112) are arranged on the upper cover plate (11), and a test paper groove (2) is arranged in the lower cover plate (12); a sliding cover (21) is arranged on the test paper groove (2), the sliding cover (21) is in sliding connection with the test paper groove (2), an observation window (211) is arranged on the sliding cover (21), the observation window (211) is arranged corresponding to the window (111), and a sealing film is arranged on the observation window (211); the detection test paper (3) is placed in the test paper groove (2), the detection test paper (3) comprises a PVC lining plate (35), a sample pad (31), a colloidal gold combined pad (32), a nitrocellulose membrane (33) and a water absorption pad (34), the sample pad (31), the colloidal gold combined pad (32), the nitrocellulose membrane (33) and the water absorption pad (34) are sequentially and partially overlapped and laid on the PVC lining plate (35), the colloidal gold combined pad (32) is coated with a dengue virus resistant monoclonal antibody 1, an observation window (211) is arranged corresponding to the nitrocellulose membrane (33), the nitrocellulose membrane (33) is provided with a detection line (331) and a quality control line (332), the detection line (331) is arranged on one side close to the colloidal gold combined pad (32), and the quality control line (332) is arranged on one side close to the water absorption pad (34); the lower cover plate (12) is also internally provided with a self-heating bag (5), and the self-heating bag (5) is provided with a pull buckle (51).
2. The dengue colloidal gold assay card of claim 1, wherein: a support column (4) is arranged in the lower cover plate (12), and the test paper groove (2) is fixed on the support column (4).
3. The dengue colloidal gold assay card of claim 1, wherein: the sample pad (31) is a glass fiber film, a polyester film or a filter paper fiber.
4. The dengue colloidal gold assay card of claim 1, wherein: the window (111) is provided with a sealing film, and the water injection hole (112) is provided with a sealing cover.
5. The dengue colloidal gold assay card of claim 1, wherein: the detection line (331) is coated with an anti-dengue virus monoclonal antibody 2, and the quality control line (332) is coated with a goat anti-mouse IgG antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920617138.4U CN210347670U (en) | 2019-04-30 | 2019-04-30 | Dengue fever colloidal gold detection card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920617138.4U CN210347670U (en) | 2019-04-30 | 2019-04-30 | Dengue fever colloidal gold detection card |
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| CN210347670U true CN210347670U (en) | 2020-04-17 |
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| CN201920617138.4U Active CN210347670U (en) | 2019-04-30 | 2019-04-30 | Dengue fever colloidal gold detection card |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965175A (en) * | 2020-08-12 | 2020-11-20 | 武汉普利赛斯科技有限公司 | Preparation method of AIDS blood test paper |
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- 2019-04-30 CN CN201920617138.4U patent/CN210347670U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965175A (en) * | 2020-08-12 | 2020-11-20 | 武汉普利赛斯科技有限公司 | Preparation method of AIDS blood test paper |
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