CN210340892U

CN210340892U - DNA filter equipment

Info

Publication number: CN210340892U
Application number: CN201921083407.XU
Authority: CN
Inventors: 黄杰
Original assignee: Shangqiu Normal University
Current assignee: Shangqiu Normal University
Priority date: 2019-07-11
Filing date: 2019-07-11
Publication date: 2020-04-17
Anticipated expiration: 2029-07-11

Abstract

The utility model belongs to the technical field of experimental consumables, and particularly discloses a DNA filtering device, which comprises an EP filtering pipe, a filtering screen and a pressurizing piece, wherein the EP filtering pipe comprises a first pipe body and a cover body, the cover body is provided with an air passing hole, the air passing hole is provided with a closing piece, and the filtering screen is detachably connected in the first pipe body; the pressurizing piece comprises a second pipe body and a piston piece, and an air outlet is formed in the second pipe body. The device has high filtering speed on the liquid containing DNA, can effectively shorten the waiting and filtering time of an experimenter, and advances the experimental process of the experimenter.

Description

DNA filter equipment

Technical Field

The utility model discloses the application belongs to experiment consumptive material technical field, specifically discloses a DNA filter equipment.

Background

In biology, biochemistry, pharmacology, and other disciplines, experimenters often need to perform operations of filtering a small amount of liquid containing flocculent DNA during experiments, so as to separate the liquid from the DNA. The current commercial DNA filtration devices are EP tubes with built-in screens. However, the filtering speed of the filtering device is very slow, and a laboratory technician is required to wait for a long time, which greatly influences the experimental process of the laboratory technician.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a DNA filter equipment that filter speed is fast.

In order to achieve the above purpose, the technical scheme of the utility model is that:

a DNA filtering device comprises an EP filtering pipe, a filtering screen and a pressurizing piece, wherein the EP filtering pipe comprises a first pipe body with an upward opening and a cover body capable of being buckled on the first pipe body, an air passing hole is formed in the cover body, a sealing piece capable of sealing the air passing hole is arranged on the air passing hole, and the filtering screen is detachably connected in the first pipe body; the pressurizing piece comprises a second pipe body and a piston piece capable of discharging gas in the second pipe body, and the second pipe body is provided with a gas outlet.

The working principle and the beneficial effects of the technical scheme are as follows:

when the air vent is used, the sealing piece on the air vent is taken down firstly, so that the sealing piece does not seal the air vent any more; then adding liquid containing DNA into the first tube body (at the moment, the liquid is positioned above the filter screen), and buckling the cover body at the opening of the first tube body; then aim at the gas outlet of second body and the gas hole of passing of lid, control piston spare makes the gas in the second body discharge from the gas outlet, then in the gas hole entering first body of passing through the lid, realize the pressurization to first body, the separation of acceleration liquid and DNA filters. In addition, during operation, the laboratory technician can control the pressure in the first tube body by himself to accelerate the separation and filtration of the liquid and the DNA, for example, the moving distance of the piston member can be controlled, or the air outlet of the second tube body can be aligned to the air passing hole of the cover body discontinuously.

Further, the second pipe body is arranged vertically downwards, a gas injection head communicated with the gas outlet is arranged on the second pipe body, and a one-way gas outlet valve which only allows gas to be discharged from the second pipe body is arranged in the gas injection head; the side wall of the second pipe body is provided with an air inlet, and a one-way air inlet valve which only allows air to enter the second pipe body is arranged in the air inlet. The piston piece is controlled to move towards the direction far away from the gas outlet (or the gas injection head), and gas enters the second pipe body through the gas inlet; the control piston part moves towards the direction close to the gas outlet (or the gas injection head), and gas in the second pipe body is discharged from the gas outlet (or the gas injection head), namely the arrangement of the gas injection head, the one-way gas outlet valve, the gas inlet and the one-way gas inlet valve can further facilitate the experimenter to control the pressure in the first pipe body discontinuously.

Further, the closing part comprises a limiting part and a closing part capable of closing the air passing hole, the central line of the limiting part is parallel to the central line of the closing part, a sliding hole is formed in the cover body, a first bulge capable of sliding in the sliding hole is arranged at the top of the limiting part, the slidable distance of the first bulge in the sliding hole is larger than the distance of the closing part extending into the air passing hole, and the air passing hole in the cover body can be conveniently closed.

Furthermore, the longitudinal section of the sealing piece is of a mountain-like shape, the number of the limiting parts is two, and the two limiting parts are respectively positioned on two sides of the sealing part. The limiting parts on the two sides of the sealing part can ensure the stability of the movement of the sealing part and ensure the complete sealing of the sealing part to the air passing hole.

The support frame comprises a vertical rod, a first horizontal rod and a second horizontal rod positioned below the first horizontal rod, one end of the first horizontal rod can vertically slide on the vertical rod, and the other end of the first horizontal rod is fixedly connected with the second pipe body; one end of the second horizontal rod is fixedly connected to the vertical rod, and the other end of the second horizontal rod is fixedly connected with the ferrule. When the laboratory technician is operating, at first place the EP filter tube on the lasso, then adjust the height of first horizontal pole, just can make the gas outlet of second body aim at the gas passing hole of lid.

Furthermore, a strip-shaped hole is formed in the vertical rod, and one end of the first horizontal rod can be fixedly connected to any position of the strip-shaped hole through a bolt and a nut. The height of the first horizontal rod can be conveniently adjusted by an experimenter.

Furthermore, the support frame still includes the base, and it has the bolt hole to open on the base, is convenient for to the fixed of support frame.

Drawings

Fig. 1 is a longitudinal sectional view of an embodiment of the present invention;

fig. 2 is a partial longitudinal cross-sectional view of an EP filter tube according to an embodiment of the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

fig. 4 is a longitudinal sectional view of a filter screen according to an embodiment of the present invention;

fig. 5 is a longitudinal sectional view of a pressing member according to an embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: EP filter tube 1, first tube 11, lid 12, air passing hole 121, sliding hole 13, pressure part 3, second tube 31, air inlet 311, piston 32, piston rod 33, gas injection head 34, filter screen 4, closing part 5, spacing part 51, closing part 52, first arch 53, support frame 6, base 61, vertical rod 62, strip-shaped hole 621, first horizontal rod 63, second horizontal rod 64, and ferrule 641.

As shown in fig. 1-5: a DNA filter device comprises an EP filter tube 1, a filter screen 4, a pressurizing piece 3 and a support frame 6.

The EP filter tube 1 includes a first tube 11 opened upward and a cover 12 capable of being fastened to an opening of the first tube 11, and the first tube 11 is hinged to the cover 12. The lid 12 is provided with a vent hole 121, and the vent hole 121 is provided with a sealing member 5 capable of sealing the vent hole 121.

The sealing member 5 comprises a horizontal part, two limiting parts 51 and a sealing part 52 capable of sealing the air passing hole 121, the central line of the limiting part 51 is parallel to the central line of the sealing part 52, the longitudinal section of the sealing member 5 is in a mountain-like shape, the number of the limiting parts 51 is two, the two limiting parts 51 are respectively arranged on two sides of the sealing part 52, and the limiting parts 51 and the sealing part 52 are integrally connected to the horizontal part. The cover body 12 is provided with a sliding hole 13, the top of the limiting part 51 is provided with a first bulge 53 which can slide in the sliding hole 13, the slidable distance of the first bulge 53 in the sliding hole 13 is larger than the distance of the closed part 52 extending into the air passing hole 121, namely, when the upper surface of the first bulge 53 is contacted with the upper surface of the sliding hole 13, the closed part 52 extends into the air passing hole 121 and is sealed and in interference fit with the air passing hole 121; when the lower surface of the first projection 53 is in contact with the lower surface of the slide hole 13, the closing portion 52 does not extend through the air hole 121.

The pressurizing part 3 comprises a second pipe body 31, a piston part and an air injection head 34 which can extend into the air hole 121, the piston part comprises a piston 32 and a piston rod 33 which are integrally connected, the second pipe body 31 is vertically arranged downwards, the bottom of the second pipe body 31 is provided with an air outlet, the bottom of the second pipe body 31 is provided with the air injection head 34 communicated with the air outlet, and the air injection head 34 is internally provided with a one-way air outlet valve which only allows air to be discharged from the second pipe body 31; the sidewall of the second tube 31 is provided with an air inlet 311, and the air inlet 311 is provided with a one-way air inlet valve for allowing air to enter the second tube 31.

The supporting frame 6 comprises a base 61, a vertical rod 62, a first horizontal rod 63 and a second horizontal rod 64 positioned below the first horizontal rod 63, a strip-shaped hole 621 is formed in the vertical rod 62, one end of the first horizontal rod 63 can vertically slide on the strip-shaped hole 621, one end of the first horizontal rod 63 can be fixedly connected to any position on the strip-shaped hole 621 through a bolt and a nut, and the other end of the first horizontal rod 63 is fixedly connected with the second pipe body 31 through a bolt; one end of the second horizontal rod 64 is fixedly connected to the vertical rod 62 by a bolt, and the other end of the second horizontal rod 64 is fixedly connected to the collar 641 by a bolt.

The connection can be dismantled in first body 11 to filter screen 4, and when filter screen 4 was placed in first body 11, the top of filter screen 4 was hung at the opening part of first body 11, and lid 12 lock is sealed and interference fit between lid 12, filter screen 4 and the first body 11 when the opening part of first body 11.

When in use, the closing part 52 on the air passing hole 121 is pulled out from the bottom of the cover 12, so that the closing part 52 does not close the air passing hole 121, and the lower surface of the first protrusion 53 contacts with the lower surface of the sliding hole 13.

Then adding liquid containing DNA into the first tube 11 (at this time, the liquid is above the filter screen 4), and fastening the cover 12 at the opening of the first tube 11; then, the air outlet of the second tube 31 is aligned with the air passing hole 121 of the cover 12, and the piston rod 33 is vertically pressed downward, so that the air in the second tube 31 is discharged from the air outlet, and then enters the first tube 11 through the air passing hole 121 of the cover 12, thereby pressurizing the first tube 11 and accelerating the separation and filtration of the liquid and the DNA. In addition, during operation, the laboratory technician can control the pressure inside the first tube 11 to accelerate the separation and filtration of the liquid and the DNA, for example, can control the moving distance of the piston member, or intermittently align the air outlet of the second tube 31 with the air vent 121 of the cover 12.

The above description is only an example of the present invention, and the common general knowledge of the known specific structures and characteristics of the embodiments is not described herein. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent.

Claims

1. A DNA filtering device is characterized by comprising an EP filtering pipe, a filtering screen and a pressurizing piece, wherein the EP filtering pipe comprises a first pipe body with an upward opening and a cover body capable of being buckled on the first pipe body; the pressurizing piece comprises a second pipe body and a piston piece capable of discharging gas in the second pipe body, and the second pipe body is provided with a gas outlet.

2. The DNA filtering device according to claim 1, wherein the second pipe is arranged vertically downward, and a gas injection head is provided on the second pipe and communicates with the gas outlet, and a one-way gas outlet valve is provided in the gas injection head for allowing only gas to be discharged from the second pipe; the side wall of the second pipe body is provided with an air inlet, and a one-way air inlet valve which only allows air to enter the second pipe body is arranged in the air inlet.

3. The DNA filtering device according to claim 2, wherein the closing member comprises a position-limiting part and a closing part capable of closing the air passing hole, the center line of the position-limiting part is parallel to the center line of the closing part, the cover body is provided with a sliding hole, the top of the position-limiting part is provided with a first protrusion capable of sliding in the sliding hole, and the slidable distance of the first protrusion in the sliding hole is greater than the distance of the closing part extending into the air passing hole.

4. The DNA filtering device according to claim 3, wherein the longitudinal cross-sectional shape of the closing member is a mountain-like shape, the number of the stopper portions is two, and the two stopper portions are respectively disposed on both sides of the closing member.

5. The DNA filtering device according to claim 4, further comprising a support frame, wherein the support frame comprises a vertical rod, a first horizontal rod and a second horizontal rod below the first horizontal rod, one end of the first horizontal rod can slide vertically on the vertical rod, and the other end of the first horizontal rod is fixedly connected with the second tube body; one end of the second horizontal rod is fixedly connected to the vertical rod, and the other end of the second horizontal rod is fixedly connected with the ferrule.

6. The DNA filtering device according to claim 5, wherein the vertical rod is provided with a strip-shaped hole, and one end of the first horizontal rod can be fixedly connected to any position of the strip-shaped hole through a bolt and a nut.

7. The DNA filtering device according to claim 6, wherein the support frame further comprises a base, and the base is provided with bolt holes.