CN210314253U - Novel puncture inoculation device for seed tank - Google Patents
Novel puncture inoculation device for seed tank Download PDFInfo
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- CN210314253U CN210314253U CN201920745511.4U CN201920745511U CN210314253U CN 210314253 U CN210314253 U CN 210314253U CN 201920745511 U CN201920745511 U CN 201920745511U CN 210314253 U CN210314253 U CN 210314253U
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- seed
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Abstract
The utility model discloses a novel puncture inoculation device for a seed tank, which comprises a seed bottle, wherein the bottleneck of the seed bottle is in threaded fit connection with a bottle cap, the bottle cap is provided with a gas pipe and a liquid suction pipe in a penetrating way, one end of the gas pipe extends into the seed bottle, and the other end is sequentially connected with a silica gel hose and a needle filter in series; one end of the pipette extends into the seed bottle, and the other end of the pipette is connected with an infusion hose; one end of the transfusion hose is connected with the pipette, and the other end of the transfusion hose is connected with the puncture contact pin; a peristaltic pump is arranged on the pipeline of the liquid suction pipe; a liquid inlet is arranged at the top of the seed tank, and a rubber plug is filled in the liquid inlet; the puncture needle head can puncture the rubber plug to enter the seed tank; the utility model overcomes various unfavorable factors of traditional flame circle or differential pressure inoculation, easy operation during the inoculation can not appear the refluence phenomenon, guarantees the inoculation effect.
Description
Technical Field
The utility model relates to a microbial seed inoculation technical field specifically is a novel dona tub puncture inoculation device.
Background
In the field of inoculation of seed tanks in the microbial fermentation industry, the traditional inoculation mode is flame ring inoculation or differential pressure inoculation, and is only suitable for seed tanks with the volume less than 3 tons. For a large seed tank, particularly a seed tank with the volume of more than 10 tons, air backflow is easily caused to cause bacterial contamination due to large ventilation volume and large difficulty in operation of a flame or differential pressure inoculation mode.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel dona tub puncture inoculation device, the device easy operation when the inoculation, the refluence phenomenon can not appear, guarantees the inoculation effect.
The utility model provides a technical scheme that its technical problem adopted is:
a novel puncture inoculation device for a seed tank comprises a seed bottle, wherein a bottle cap is connected with the mouth of the seed bottle in a threaded fit manner, an air pipe and a liquid suction pipe are arranged in the bottle cap in a penetrating manner, one end of the air pipe extends into the seed bottle, and the other end of the air pipe is sequentially connected with a silica gel hose and a needle filter in series; one end of the pipette extends into the seed bottle, and the other end of the pipette is connected with an infusion hose; one end of the transfusion hose is connected with the pipette, and the other end of the transfusion hose is connected with the puncture contact pin; a peristaltic pump is arranged on the pipeline of the liquid suction pipe;
a liquid inlet is formed in the top of the seed tank, and a rubber plug is filled in the liquid inlet; the puncture needle head can puncture the rubber plug to enter the seed tank.
Furthermore, the tail portion of the puncture contact pin is sleeved with an annular needle base, a locking nut is sleeved on the periphery of the needle base, and the locking nut can be in threaded fit with the liquid inlet.
The utility model has the advantages that the peristaltic pump pumps the seed liquid in the seed bottle into the seed tank to realize inoculation, the matching form of the puncture contact pin and the rubber plug can ensure good sealing performance, and the air pipe, the silica gel hose and the needle head filter can balance the air pressure in the seed bottle with the external air pressure; the utility model overcomes various unfavorable factors of traditional flame circle or differential pressure inoculation, easy operation during the inoculation can not appear the refluence phenomenon, guarantees the inoculation effect.
Drawings
The invention will be further described with reference to the following figures and examples:
fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
As shown in fig. 1, the utility model provides a novel seeding tank puncture inoculation device, which comprises a seeding bottle 1, wherein the bottleneck of the seeding bottle 1 is connected with a bottle cap 2 in a threaded fit manner, a gas pipe 3 and a liquid suction pipe 4 are arranged on the bottle cap 2 in a penetrating manner, one end of the gas pipe 3 extends into the seeding bottle 1, the other end is connected with a silica gel hose 5, one end of the silica gel hose 5 is connected with the gas pipe 3, and the other end is connected with a needle head filter 6; the needle filter 6 may be Sartorius Minisart SRP (hydrophibic PTFE) 25 mm.
One end of the pipette 4 extends into the seed bottle 1, and the other end is connected with an infusion hose 7; one end of the transfusion hose 7 is connected with the pipette 4, and the other end is connected with the puncture pin 8; the pipeline of the liquid suction pipe 4 is provided with a peristaltic pump 9.
A liquid inlet 11 is arranged at the top of the seeding tank 10, and a rubber plug 12 is filled in the liquid inlet 11; the head of the piercing pin 8 can pierce the rubber stopper 12 into the seed can 10.
The tail of the puncture needle 8 is sleeved with an annular needle seat 13, the periphery of the needle seat 13 is sleeved with a locking nut 14, and the locking nut 14 can be in threaded fit with the liquid inlet 11.
When in inoculation, the peristaltic pump 9 is started, the peristaltic pump 9 pumps seed liquid in the seed bottle 1 into the seed tank 10 to realize inoculation, the matching form of the puncture needle 8 and the rubber plug 12 can ensure good tightness, and the air pipe, the silica gel hose and the needle filter can balance the air pressure in the seed bottle with the external air pressure; the utility model overcomes various unfavorable factors of traditional flame circle or differential pressure inoculation, easy operation during the inoculation can not appear the refluence phenomenon, guarantees the inoculation effect.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way; the invention is not limited to the embodiments described herein, but is capable of other embodiments according to the invention, and may be used in various other applications, including, but not limited to, industrial. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments by the technical entity of the present invention all still belong to the protection scope of the technical solution of the present invention.
Claims (2)
1. A novel puncture inoculation device for a seed tank is characterized by comprising a seed bottle, wherein a bottle cap is connected to the opening of the seed bottle in a threaded fit manner, an air pipe and a liquid suction pipe are arranged on the bottle cap in a penetrating manner, one end of the air pipe extends into the seed bottle, and the other end of the air pipe is sequentially connected with a silica gel hose and a needle filter in series; one end of the pipette extends into the seed bottle, and the other end of the pipette is connected with an infusion hose; one end of the transfusion hose is connected with the pipette, and the other end of the transfusion hose is connected with the puncture contact pin; a peristaltic pump is arranged on the pipeline of the liquid suction pipe;
a liquid inlet is formed in the top of the seed tank, and a rubber plug is filled in the liquid inlet; the puncture needle head can puncture the rubber plug to enter the seed tank.
2. The novel seeding tank puncture inoculation device as claimed in claim 1, wherein the tail of the puncture needle is sleeved with an annular needle base, the periphery of the needle base is sleeved with a locking nut, and the locking nut can be in threaded fit with the liquid inlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920745511.4U CN210314253U (en) | 2019-05-23 | 2019-05-23 | Novel puncture inoculation device for seed tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920745511.4U CN210314253U (en) | 2019-05-23 | 2019-05-23 | Novel puncture inoculation device for seed tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210314253U true CN210314253U (en) | 2020-04-14 |
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ID=70139830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920745511.4U Active CN210314253U (en) | 2019-05-23 | 2019-05-23 | Novel puncture inoculation device for seed tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210314253U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112831399A (en) * | 2021-02-24 | 2021-05-25 | 梅里医疗科技(洋浦)有限责任公司 | Automatic detection reagent bottle group, kit, reagent bin and detection method for intelligent hospital |
-
2019
- 2019-05-23 CN CN201920745511.4U patent/CN210314253U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112831399A (en) * | 2021-02-24 | 2021-05-25 | 梅里医疗科技(洋浦)有限责任公司 | Automatic detection reagent bottle group, kit, reagent bin and detection method for intelligent hospital |
| CN112831399B (en) * | 2021-02-24 | 2024-01-05 | 通用技术集团健康管理科技有限公司 | Nucleic acid detection kit, kit bin and detection method for full-automatic chemiluminescence immunoassay analyzer in intelligent hospital |
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