CN210314083U - Large-scale high-efficient ultrafiltration device of PKR phosphorylation antibody - Google Patents

Abstract

The utility model relates to an ultrafiltration device technical field, and a large amount of high-efficient ultrafiltration device of PKR phosphorylation antibody is disclosed, backup pad including two symmetry settings, all through the common fixedly connected with feeder hopper of backup pad between the lateral wall that two backup pads are in opposite directions and are close to, the bottom fixedly connected with connecting pipe of feeder hopper, through the common fixedly connected with ultrafiltration box of two adjustment mechanism between two backup pads, the fixed intercommunication in bottom of ultrafiltration box has the drain pipe, the fixed intercommunication in top of ultrafiltration box has the feed liquor pipe, and the feed liquor pipe passes through connection structure and is connected with the connecting pipe, fixedly connected with section of thick bamboo of feed liquor that the round platform type is personally submitted to fixedly connected with on the tank wall in the top of ultrafiltration box, the bottom fixedly connected with connecting cylinder of a feed liquor section of thick bamboo, the bottom of connecting cylinder. The utility model discloses use solution to produce centrifugal force as power to utilize centrifugal force to improve the efficiency of solution ultrafiltration, be convenient for simultaneously to strain a section of thick bamboo and the filter disc clears up.

Description

Large-scale high-efficient ultrafiltration device of PKR phosphorylation antibody

Technical Field

The utility model relates to an ultrafiltration device technical field especially relates to a large amount of high-efficient ultrafiltration devices of PKR phosphorylation antibody.

Background

There are many operation modes and auxiliary equipments in the purification process of PKR phosphorylated antibody, among which ultrafiltration is a more common way of preparing antibody. The ultrafiltration technology is generally a membrane separation technology, and is mainly used for separating fine particles and dissolved molecules in a liquid, and the separation is mainly performed according to the size of the molecules, but the conventional ultrafiltration device performs ultrafiltration by depending on the downward flowing property of the liquid, so that the power is insufficient, the separation time is long, and the efficiency is low.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problems of long time consumption and low efficiency of the separation of the traditional PKR phosphorylation antibody ultrafiltration device in the prior art, and providing a large amount of high-efficiency PKR phosphorylation antibody ultrafiltration device.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a large-amount efficient ultrafiltration device for PKR phosphorylated antibodies comprises two symmetrically arranged support plates, wherein a feed hopper is fixedly connected between two opposite and close side walls of the two support plates through the support plates, a connecting pipe is fixedly connected to the bottom of the feed hopper, an ultrafiltration box is fixedly connected between the two support plates through two adjusting mechanisms, a liquid outlet pipe is fixedly communicated with the bottom of the ultrafiltration box, a liquid inlet pipe is fixedly communicated with the top of the ultrafiltration box and is connected with the connecting pipe through a connecting structure, a liquid inlet barrel with a circular truncated cone-shaped section is fixedly connected to the inner box wall of the top of the ultrafiltration box, a connecting barrel is fixedly connected to the bottom of the liquid inlet barrel, a filter barrel is rotatably connected to the bottom of the connecting barrel through a sealing bearing in a sealing manner, the sealing bearing is fixedly arranged in the connecting barrel, and a filter disc is fixedly connected to the bottom of the filter barrel, the filter disc and the filter cartridge are made of ultrafiltration membranes, a support frame with a cross-shaped section is fixedly connected to the inner cartridge wall of the connecting cartridge close to the top, a rotating rod is rotatably connected to the support frame through a first bearing, the first bearing is fixedly arranged in the support frame, the lower end of the rotating rod extends towards the inside of the filter disc and is rotatably connected with the filter disc through a second bearing, the second bearing is fixedly arranged in the filter disc and is located on the opposite side of the rotating rod in the filter cartridge, the first fan blades are symmetrically and fixedly connected to the opposite side of the rotating rod, and the second fan blades are symmetrically and fixedly connected to the opposite side of the rotating rod above the support frame.

Preferably, adjustment mechanism includes that the symmetry sets up the spout on two backup pads lateral walls in opposite directions, sliding connection has the slider in the spout, the one end that the slider is close to the ultrafiltration case is rotated through the third bearing and is connected with the pivot, and the third bearing is fixed to be established in the slider, the one end and the ultrafiltration case fixed connection that the pivot is close to the ultrafiltration case, the bottom fixedly connected with spring of slider, and the lower extreme of spring and the bottom cell wall fixed connection of spout, connection structure includes threaded sleeve and fixed connection just is cylindric sealed the pad in the connecting pipe bottom, all fixed being equipped with the screw thread on the outer pipe wall of connecting pipe, feed liquor pipe, drain pipe, threaded sleeve passes through the common threaded connection of screw thread and feed liquor pipe and connecting pipe.

Preferably, the outer cylinder wall of the threaded sleeve is fixedly provided with anti-skid threads.

Preferably, a plurality of bumps are fixedly connected to the ultrafiltration box.

Preferably, the bottom of the supporting plate is fixedly connected with a rubber pad.

Preferably, the support plate, the ultrafiltration box, the feed hopper, the liquid inlet pipe and the liquid outlet pipe are all coated with antirust paint.

Preferably, a protective cover is mounted on the feeding hopper.

Preferably, the connecting pipe is provided with a limiting groove with an annular section on the outer pipe wall close to the top, the inner cylinder wall of the threaded sleeve is fixedly connected with a limiting block matched with the limiting groove, and the limiting block is connected in the limiting groove in a sliding manner.

Preferably, a ball groove is formed in one end, close to the bottom of the sliding groove, of the sliding block, a ball is connected in the ball groove in a rolling mode, and one end, far away from the bottom of the ball groove, of the ball penetrates through a groove opening of the ball groove and extends outwards, and is abutted to the bottom of the sliding groove.

Compared with the prior art, the utility model provides a large amount of high-efficient ultrafiltration device of PKR phosphorylation antibody possesses following beneficial effect:

1. this a large amount of high-efficient ultrafiltration device of PKR phosphorylation antibody, through setting up the feeder hopper, the connecting pipe, the feed liquor pipe, connecting device, the ultrafiltration box, advance the liquid section of thick bamboo, the cartridge filter, the filter disc, the bull stick, first flabellum and second flabellum, when carrying out the purification to PKR phosphorylation antibody, inject PKR phosphorylation antibody solution into the feeder hopper, solution flows into the ultrafiltration box through the liquid pipe, when flowing out in advance the liquid section of thick bamboo to the connecting cylinder, because advance the structure of liquid section of thick bamboo to the round platform type, and then the solution velocity of flow that flows into in the connecting cylinder is very fast, it is rotatory to drive the second flabellum, the second flabellum makes the bull stick rotatory, and then make first flabellum, the filter disc is all rotatory with straining a section of thick bamboo, and then the solution that the drive is located to strain a section of thick.

2. This a large amount of high-efficient ultrafiltration device of PKR phosphorylation antibody, through setting up the spout, the slider, the apparatus further comprises a rotating shaft, a spring, threaded sleeve and sealed pad, when needing to strain a section of thick bamboo and filter disc to clear up, rotatory threaded sleeve makes threaded sleeve upward movement, and then feed liquor pipe separates with the connecting pipe, use the pivot as the rotatory ultrafiltrating chamber of axle, when the axis of drain pipe and connecting pipe overlaps, will seal up pad and drain pipe laminating, at this moment, under the spring force effect, it is inseparable with the drain pipe laminating to seal up, rotatory threaded sleeve downwards, be in the same place threaded sleeve and drain pipe swivelling joint, add the washing liquid in the feeder hopper, the washing liquid is to straining a section of thick bamboo and filter disc backwash, will remain impurity washing, be convenient for wash straining a.

The part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, the utility model discloses use solution to produce centrifugal force as power to utilize centrifugal force to improve the efficiency of solution ultrafiltration, be convenient for simultaneously to straining a section of thick bamboo and filter disc and clear up.

Drawings

Fig. 1 is a schematic structural diagram of a large-scale high-efficiency ultrafiltration device for a PKR phosphorylated antibody according to the present invention;

FIG. 2 is an enlarged view of portion A of FIG. 1;

FIG. 3 is an enlarged view of portion B of FIG. 1;

fig. 4 is a sectional view taken along line C-C in fig. 1.

In the figure: the device comprises a supporting plate 1, a feeding hopper 2, a connecting pipe 3, a ultrafiltration box 4, a liquid outlet pipe 5, a liquid inlet pipe 6, a liquid inlet cylinder 7, a connecting cylinder 8, a filter cylinder 9, a filter disc 10, a support frame 11, a rotating rod 12, a first fan blade 13, a second fan blade 14, a sliding groove 15, a sliding block 16, a rotating shaft 17, a spring 18, a threaded sleeve 19, a sealing gasket 20, a limiting groove 21, a limiting block 22, a ball groove 23 and balls 24.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-4, a large amount of high-efficiency ultrafiltration device for PKR phosphorylated antibodies comprises two support plates 1 which are symmetrically arranged, wherein a feed hopper 2 is fixedly connected between opposite and close side walls of the two support plates 1 through the support plates, a connecting pipe 3 is fixedly connected at the bottom of the feed hopper 2, an ultrafiltration box 4 is fixedly connected between the two support plates 1 through two adjusting mechanisms, a liquid outlet pipe 5 is fixedly communicated at the bottom of the ultrafiltration box 4, a liquid inlet pipe 6 is fixedly communicated at the top of the ultrafiltration box 4, the liquid inlet pipe 6 is connected with the connecting pipe 3 through a connecting structure, a liquid inlet cylinder 7 with a circular truncated cone-shaped section is fixedly connected on the inner box wall at the top of the ultrafiltration box 4, a connecting cylinder 8 is fixedly connected at the bottom of the liquid inlet cylinder 7, a filter cylinder 9 is rotatably connected at the bottom of the connecting cylinder 8 through a sealing bearing in a sealing, the bottom of the filter cylinder 9 is fixedly connected with a filter disc 10, the filter disc 10 and the filter cylinder 9 are both made of ultrafiltration membranes, the inner cylinder wall of the connecting cylinder 8 close to the top is fixedly connected with a supporting frame 11 with a cross-shaped section, the supporting frame 11 is rotatably connected with a rotating rod 12 through a first bearing, the first bearing is fixedly arranged in the supporting frame 11, the lower end of the rotating rod 12 extends into the filter disc 10 and is rotatably connected with the filter disc 10 through a second bearing, the second bearing is fixedly arranged in the filter disc 10, first fan blades 13 are symmetrically and fixedly connected on the rod wall opposite to the rotating rod 12 in the filter cylinder 9, second fan blades 14 are symmetrically and fixedly connected on the rod wall opposite to the rotating rod 12 above the supporting frame 11, when the PKR phosphorylated antibody is purified, the PKR phosphorylated antibody solution is injected into the feed hopper 2, the solution flows into the ultrafiltration box 4 through a liquid pipe 6 and flows out through the liquid inlet cylinder 7 to the connecting cylinder 8, due to the fact that the liquid inlet cylinder 7 is of a circular truncated cone structure, the flow rate of the solution flowing into the connecting cylinder 8 is high, the second fan blades 14 are driven to rotate, the second fan blades 14 enable the rotating rod 12 to rotate, the first fan blades 13, the filter disc 10 and the filter cylinder 9 are further enabled to rotate, the solution in the filter cylinder 9 is driven to flow, centrifugal force is generated, and the efficiency of ultrafiltration of the PKR phosphorylation antibody solution is improved under the action of the centrifugal force.

The adjusting mechanism comprises sliding chutes 15 symmetrically arranged on opposite side walls of the two support plates 1, sliding blocks 16 are connected in the sliding chutes 15 in a sliding manner, one ends of the sliding blocks 16, which are close to the ultrafiltration box 4, are rotatably connected with rotating shafts 17 through third bearings, the third bearings are fixedly arranged in the sliding blocks 16, one ends of the rotating shafts 17, which are close to the ultrafiltration box 4, are fixedly connected with the ultrafiltration box 4, springs 18 are fixedly connected with the bottoms of the sliding blocks 16, the lower ends of the springs 18 are fixedly connected with the bottom groove walls of the sliding chutes 15, the connecting structure comprises threaded sleeves 19 and cylindrical sealing gaskets 20 fixedly connected at the bottoms of the connecting pipes 3, threads are fixedly arranged on the outer pipe walls of the connecting pipes 3, the liquid inlet pipes 6 and the liquid outlet pipes 5, the threaded sleeves 19 are in threaded connection with the liquid inlet pipes 6 and the connecting pipes 3 through threads, and when the filter cartridge 9 and the filter disc 10, and then feed liquor pipe 6 separates with connecting pipe 3, use pivot 17 as the rotatory ultra-filtration case 4 of axle, when drain pipe 5 overlaps with the axis of connecting pipe 3, with sealed 20 and drain pipe 5 laminating, at this moment, under the spring action of 18 elasticity, sealed 20 with drain pipe 5 laminating closely, screw sleeve 19 is rotated downwards, with screw sleeve 19 and drain pipe 5 swivelling joint together, add the washing liquid in feeder hopper 2, the washing liquid is to straining a section of thick bamboo 9 and filter disc 10 back flush, wash remaining impurity, be convenient for strain a section of thick bamboo 9 and filter disc 10 and wash.

The fixed anti-skidding line that is equipped with on the outer section of thick bamboo wall of threaded sleeve 19, when needing rotatory threaded sleeve 19, put both hands on the outer section of thick bamboo wall of threaded sleeve 19, both hands are gripped, at this moment, because the existence of anti-skidding line, increased the frictional force of user's both hands with the contact of the 19 outer sections of thick bamboo walls of threaded sleeve, and then convenient to use person rotates threaded sleeve 19, lets the more simple and convenient rotatory threaded sleeve of user.

A plurality of lugs of fixedly connected with on the ultrafiltration case 4, rotatory ultrafiltration case 4 when needing clearance to strain a section of thick bamboo 9 and filter disc 10, arbitrary lug is grabbed to both hands, rotatory ultrafiltration case 4, when rotatory certain degree, change the hand and grab arbitrary another suitable lug, continue rotatoryly, rotatory ultrafiltration case 4 of being convenient for, the inconvenient quilt of surface that can not because ultrafiltration case 4 during the rotation is grabbed and the condition of rotatory ultrafiltration case 4 of being not convenient for takes place.

The bottom fixedly connected with rubber pad of backup pad 1, the rubber pad is very soft, and on the one hand when the device is placed at hollow subaerial, soft rubber pad and hollow unevenness's ground overlap, makes stable the placing of device, and on the other hand, when the device shakes, soft rubber pad plays the cushioning effect, reduces the vibrations of device, protects the device.

Backup pad 1, ultrafiltration case 4, feeder hopper 2, all be scribbled the anti rust paint on feed liquor pipe 6 and the drain pipe 5, the anti rust paint with backup pad 1, ultrafiltration case 4, feeder hopper 2, feed liquor pipe 6 and drain pipe 5 are kept apart with the outside air, and then steam in the external air and oxygen all can not with backup pad 1, ultrafiltration case 4, feeder hopper 2, feed liquor pipe 6 and drain pipe 5 contact, and then prevent backup pad 1, ultrafiltration case 4, feeder hopper 2, the rusty condition of feed liquor pipe 6 and drain pipe 5 from taking place.

Install the protective cover on feeder hopper 2, the fixed screw thread that is equipped with on the protective cover, fixed being equipped with on the feeder hopper 2 with protective cover assorted screw thread, protective cover and 2 threaded connection of feeder hopper, during normal use device, do not need the protective cover, take off the protective cover rotation, when not using device, install the protective cover rotation on feeder hopper 2, because the existence of protective cover, in external impurity can not the entering device, protect the device.

Connecting pipe 3 has been seted up the section on being close to the back of the body outer pipe wall at top and has been annular spacing groove 21, fixedly connected with and spacing groove 21 assorted stopper 22 on the inner tube wall of threaded sleeve 19, and stopper 22 sliding connection is in spacing groove 21, because annular stopper 22 (combine figure 4 to observe clearly) sliding connection in spacing groove 21, and then stopper 22 can not break away from spacing groove 21, and then during rotatory threaded sleeve 19, stopper 22 can be at spacing groove 21 internal rotation and the motion of rising decline, stopper 22 can not break away from spacing groove 21, and then threaded sleeve 19 is difficult separately with connecting pipe 3, spacing groove 21 cooperation stopper 22 uses the condition that can prevent threaded sleeve 19 from breaking away from connecting pipe 3 and takes place.

The ball groove 23 has been seted up to the one end that slider 16 is close to the spout 15 tank bottom, it is connected with ball 24 to roll in the ball groove 23, ball 24 is kept away from the one end of ball groove 23 tank bottom and is passed the notch of ball groove 23 and outwards extend, and offset with the tank bottom of spout 15, the existence of ball groove 23, slider 16 becomes roll connection with the sliding connection of spout 15, common knowledge knows, the frictional force that roll connection brought than sliding connection is littleer, it is more swift to make slider 16 slide in spout 15.

In the utility model, when purifying the PKR phosphorylated antibody, the PKR phosphorylated antibody solution is injected into the feed hopper 2, the solution flows into the ultrafiltration box 4 through the liquid pipe 6, and flows out into the connecting cylinder 8 through the liquid inlet cylinder 7, and then the flow rate of the solution flowing into the connecting cylinder 8 is faster due to the structure from the liquid inlet cylinder 7 to the circular truncated cone, so as to drive the second fan blade 14 to rotate, and the second fan blade 14 rotates the rotating rod 12, so that the first fan blade 13, the filter disc 10 and the filter cylinder 9 rotate, and further the solution in the filter cylinder 9 is driven to flow, thereby generating centrifugal force, and under the action of the centrifugal force, the efficiency of ultrafiltration of the PKR phosphorylated antibody solution is improved; when a filter cartridge 9 and a filter disc 10 need to be cleaned, the thread sleeve 19 is rotated to enable the thread sleeve 19 to move upwards, and further the liquid inlet pipe 6 is separated from the connecting pipe 3, the rotating shaft 17 is used as the shaft rotating ultrafiltration box 4, when the axes of the liquid outlet pipe 5 and the connecting pipe 3 are overlapped, the sealing gasket 20 is attached to the liquid outlet pipe 5, at the moment, the spring 18 is used for exerting an elastic force, the sealing gasket 20 is attached to the liquid outlet pipe 5 tightly, the thread sleeve 19 is rotated downwards, the thread sleeve 19 is connected with the liquid outlet pipe 5 in a rotating mode, cleaning liquid is added into the feed hopper 2, the cleaning liquid washes the filter cartridge 9 and the filter disc 10 in a reverse direction, residual impurities are cleaned, and the filter cartridge 9 and the filter disc 10 are convenient.

Above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the design of the present invention, equivalent replacement or change should be covered within the protection scope of the present invention.

Claims (9)

1. A large amount of high-efficient ultrafiltration devices of PKR phosphorylation antibody, includes two backup pads (1) that the symmetry set up, its characterized in that, two backup pads (1) are in opposite directions and all through the common fixedly connected with feeder hopper (2) of backup pad between the lateral wall that is close to, the bottom fixedly connected with connecting pipe (3) of feeder hopper (2), two through the common fixedly connected with ultrafiltration case (4) of two adjustment mechanism between backup pad (1), the bottom fixed intercommunication of ultrafiltration case (4) has drain pipe (5), the top fixed intercommunication of ultrafiltration case (4) has feed liquor pipe (6), and feed liquor pipe (6) are connected with connecting pipe (3) through connection structure, fixedly connected with the cross section is the liquid inlet cylinder (7) of round platform type on the top inner tank wall of ultrafiltration case (4), the bottom fixedly connected with connecting cylinder (8) of liquid inlet cylinder (7), the bottom of the connecting cylinder (8) is rotatably connected with a filter cylinder (9) in a sealing way through a sealing bearing, the sealing bearing is fixedly arranged in the connecting cylinder (8), the bottom of the filter cylinder (9) is fixedly connected with a filter disc (10), the filter disc (10) and the filter cylinder (9) are both made of ultrafiltration membranes, a support frame (11) with a cross-shaped section is fixedly connected onto the inner cylinder wall of the connecting cylinder (8) close to the top, a rotating rod (12) is rotatably connected onto the support frame (11) through a first bearing, the first bearing is fixedly arranged in the support frame (11), the lower end of the rotating rod (12) extends into the filter disc (10) and is rotatably connected with the filter disc (10) through a second bearing, the second bearing is fixedly arranged in the filter disc (10), and first fan blades (13) are symmetrically and fixedly connected onto the rod wall of the filter cylinder (9) opposite to the rotating rod (12), and the second fan blades (14) are symmetrically and fixedly connected to the back rod wall of the rotating rod (12) above the supporting frame (11).

2. The device as claimed in claim 1, wherein the adjusting mechanism comprises sliding grooves (15) symmetrically formed in opposite side walls of the two support plates (1), a sliding block (16) is slidably connected in the sliding grooves (15), a rotating shaft (17) is rotatably connected to one end, close to the ultrafiltration box (4), of the sliding block (16) through a third bearing, the third bearing is fixedly arranged in the sliding block (16), one end, close to the ultrafiltration box (4), of the rotating shaft (17) is fixedly connected with the ultrafiltration box (4), a spring (18) is fixedly connected to the bottom of the sliding block (16), the lower end of the spring (18) is fixedly connected with the bottom groove wall of the sliding groove (15), the connecting structure comprises a threaded sleeve (19) and a cylindrical sealing gasket (20) fixedly connected to the bottom of the connecting pipe (3), and the connecting pipe (3), All fixedly on the outer pipe wall of feed liquor pipe (6), drain pipe (5) be equipped with the screw thread, screw thread sleeve (19) are through screw thread and feed liquor pipe (6) and the common threaded connection of connecting pipe (3).

3. The device for the large-scale and efficient ultrafiltration of the phosphorylated antibodies of the PKR as claimed in claim 2, wherein the outer cylindrical wall of the threaded sleeve (19) is fixedly provided with anti-skid threads.

4. The device for the large-scale and high-efficiency ultrafiltration of the phosphorylated antibodies for PKR according to claim 1, wherein a plurality of bumps are fixedly connected to the ultrafiltration tank (4).

5. The high-efficiency ultrafiltration device for mass production of phosphorylated antibodies for PKR as claimed in claim 1, wherein the bottom of the support plate (1) is fixedly connected with a rubber pad.

6. The device for the large-scale and efficient ultrafiltration of the phosphorylated antibodies for PKR according to claim 1, wherein the support plate (1), the ultrafiltration tank (4), the feed hopper (2), the liquid inlet pipe (6) and the liquid outlet pipe (5) are coated with an anti-rust paint.

7. The device for the large scale high efficiency ultrafiltration of phosphorylated antibodies for PKR according to claim 1, wherein a protective cover is installed on the feeding funnel (2).

8. The device for the large-scale efficient ultrafiltration of the phosphorylated antibodies for PKR as claimed in claim 2, wherein the opposite outer tube wall of the connecting tube (3) near the top is provided with a limiting groove (21) having an annular cross section, the inner tube wall of the threaded sleeve (19) is fixedly connected with a limiting block (22) matched with the limiting groove (21), and the limiting block (22) is slidably connected in the limiting groove (21).

9. The device for the large-scale and efficient ultrafiltration of the phosphorylated antibodies for PKR according to claim 2, wherein the end of the slider (16) close to the bottom of the sliding chute (15) is provided with a ball groove (23), a ball (24) is connected in the ball groove (23) in a rolling manner, and the end of the ball (24) far away from the bottom of the ball groove (23) passes through the opening of the ball groove (23) and extends outwards, and abuts against the bottom of the sliding chute (15).

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