CN210243668U - Test piece for rapidly and quantitatively detecting tissue cell protein - Google Patents
Test piece for rapidly and quantitatively detecting tissue cell protein Download PDFInfo
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- CN210243668U CN210243668U CN201920712207.XU CN201920712207U CN210243668U CN 210243668 U CN210243668 U CN 210243668U CN 201920712207 U CN201920712207 U CN 201920712207U CN 210243668 U CN210243668 U CN 210243668U
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Abstract
The utility model relates to a test piece for fast and quantitatively detecting tissue cell protein. Specifically, the utility model provides a test strip for detecting histocyte protein, the test strip includes a substrate, from one end of the substrate to the other end direction, is equipped with sample addition area, colloidal gold area, protein detection area and water absorption area on the substrate in proper order; the protein detection area comprises a plurality of detection points, the detection points are arranged in a plurality of rows, the circle centers of the detection points in each row are mutually connected to form a straight line, and the straight line is vertical to the flowing advancing direction of the sample to be detected; the spots on the protein detection zone are presented in an alternating array. The test piece of the utility model can detect the tissue cell protein rapidly, accurately, qualitatively and quantitatively.
Description
Technical Field
The utility model relates to a detection area specifically, relates to a test piece of quick quantitative determination histocyte protein.
Background
In the past, the tumor therapy, especially the chemotherapy, has wide drug action range, kills a large number of normal cells while killing tumor cells, and causes intolerable toxic and side effects. With the rapid progress of medical research, scientists gradually develop tumor targeted therapeutic drugs with strong pertinence and small toxic and side effects. At present, the targeted therapy is more and more a development trend of tumor therapy due to good treatment effect and small toxic and side effect.
Surgery or biopsy can accelerate infiltration and diffusion of tumor cells, and the medical community consistently recognizes that timely and effective treatment is a necessary means for preventing the diffusion of the tumor cells. However, how to rapidly and accurately judge the molecular type of the tumor of a patient in clinic and further determine whether the patient is suitable for a certain targeted therapy scheme is still a problem which needs to be solved urgently in the medical field at present.
Therefore, there is a need in the art to develop a method for rapidly and quantitatively detecting the level of tissue cell protein, especially the level of tumor cell-associated protein, so as to provide guidance for the treatment of diseases.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a can be fast quantitative determination tissue cell albumen especially with the test strip of tumor cell related cell albumen.
The utility model provides a test piece for detecting tissue cell protein, which comprises a substrate 1, wherein a sample adding area 2, a colloidal gold area 3, a protein detection area and a water absorption area 4 are arranged on the substrate in sequence from one end of the substrate to the other end;
the protein detection area comprises a plurality of detection points 5, the detection points are arranged in a plurality of rows, the circle centers of the detection points in each row are mutually connected to form a straight line, and the straight line is vertical to the flowing advancing direction of a sample to be detected;
the spots on the protein detection zone are presented in an alternating array.
In another preferred embodiment, the detecting points are arranged in n +1 columns, where n is 3-30, preferably 3-20, more preferably 3-15, and even more preferably 5-12, and n is a positive integer (e.g., 1, 2, 3, 4, 9, 15, or 30, etc.).
In another preferred embodiment, the shapes of the detection points are the same or different.
In another preferred example, the shape of the detection point is a circle.
In another preferred embodiment, the inner diameters of the detection points are the same or different.
In another preferred example, the circle center distances of two adjacent detection points in each column are the same.
In another preferred embodiment, each column contains from 2 to 30, preferably from 2 to 20, more preferably from 2 to 10, most preferably from 2 to 6 detection points.
In another preferred embodiment, the difference of the number of the detection points in each column is 1-2.
In another preferred example, the straight lines formed by connecting the centers of the detection points of each row are parallel to each other.
In another preferred embodiment, the vertical distance between two adjacent columns is 1.0-1.8mm, preferably 1.2-1.6mm, more preferably 1.3-1.5 mm. In another preferred example, the distance between the centers of circles between two adjacent detection points in one column and one detection point in another adjacent column corresponding to the gap between the two adjacent detection points is equal or unequal.
In another preferred embodiment, the distance between the centers of circles of one adjacent row of two detecting points and one detecting point of another adjacent row corresponding to the gap between the two adjacent detecting points of the row is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred example, the distance between the centers of circles of the two adjacent detection points in one column and the detection point in the other adjacent column corresponding to the gap between the two adjacent detection points is equal or unequal.
In another preferred embodiment, a gap between two adjacent detecting points D1 and D2 in one column and a gap between D1 and D2 corresponds to one detecting point D3 in another adjacent column, wherein the distance between the centers of the two detecting points D1 and D3 is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm; the distance between the centers of the two detection points D2 and D3 is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred embodiment, an equilateral triangle is formed between one detection point of another adjacent column corresponding to the gap between two adjacent detection points of the column.
In another preferred example, the shape of the detection point is a circle.
In another preferred embodiment, the detecting points are arranged in n +1 columns, where n is 3-30, preferably 3-20, more preferably 3-15, and most preferably 5-12, and n is a positive integer.
In another preferred embodiment, the shape of each detection point is the same.
In another preferred embodiment, the inner diameter of each detection point is 0.5-1.2mm, preferably 0.6-1.0mm, more preferably 0.7-0.9 mm.
In another preferred embodiment, the distance between the centers of two adjacent detecting points in each row is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred embodiment, the distance between the centers of the two adjacent detecting points in one row and the detecting point in the other adjacent row corresponding to the gap therebetween is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred embodiment, the test strip further comprises a quality control region on the substrate.
In another preferred embodiment, the quality control region is located between the protein detection region and the water absorption region.
In another preferred embodiment, the colloidal gold region is loaded with one or more complexes selected from the group consisting of: colloidal gold antibody complexes, colloidal silver antibody complexes;
the colloidal gold antibody compound is formed by coupling colloidal gold and an antibody selected from the following group: a monoclonal antibody of the tissue cell protein to be detected, a polyclonal antibody of the tissue cell protein to be detected, or a combination thereof;
the colloidal silver antibody compound is formed by coupling colloidal silver and an antibody selected from the following group: a monoclonal antibody to be detected for a tissue cell protein, a polyclonal antibody to be detected for a tissue cell protein, or a combination thereof.
In another preferred example, the colloidal gold region is provided with glass fibers, and the composite is coated on the glass fibers.
In another preferred embodiment, the tissue cell protein to be detected is HER2 protein.
In another preferred embodiment, the colloidal gold region further supports an IgG colloidal gold complex, an IgG colloidal silver complex, or a combination thereof.
In another preferred embodiment, the detection spot in the protein detection zone is loaded with one or more antibodies selected from the group consisting of: monoclonal antibody resisting tissue protein to be detected and polyclonal antibody resisting tissue protein to be detected.
In another preferred embodiment, the colloidal gold region comprises a colloidal gold complex loaded with HER2 antibody, and the checkpoint comprises an anti-HER 2 antibody.
In another preferred embodiment, the protein detection area is provided with a nitrocellulose membrane, and the monoclonal antibody against the tissue protein to be detected or the polyclonal antibody against the tissue protein to be detected is coated on the glass fiber.
In another preferred example, the HER2 antibody in the HER2 antibody colloidal gold complex is a rabbit-derived anti-human HER2 antibody.
In another preferred embodiment, the sample addition part comprises a sample addition pad.
In another preferred embodiment, the absorbent region comprises absorbent paper.
The utility model discloses in a second aspect, a kit for detecting histocyte protein is provided, the kit include:
(i) a test strip for detecting a tissue cell protein according to the first aspect of the present invention;
(ii) instructions for use.
In another preferred embodiment, the instructions describe the use of the test strip for detecting a tissue cell protein according to the first aspect of the present invention for detecting a tissue cell protein.
The third aspect of the present invention provides a use of the test strip of the first aspect of the present invention in detecting tissue cell proteins.
In another preferred embodiment, the assay is an in vitro assay.
In another preferred embodiment, the detection comprises qualitative and/or quantitative detection.
In another preferred example, the detection is auxiliary detection.
In another preferred embodiment, the assay is a non-therapeutic and non-diagnostic assay.
The fourth aspect of the present invention provides a method for detecting tissue cell proteins, the method comprising using the test strip for detecting tissue cell proteins of the first aspect of the present invention to detect tissue cell proteins in a sample to be tested.
In another preferred embodiment, the assay is an in vitro assay.
In another preferred embodiment, the detection comprises qualitative and/or quantitative detection.
In another preferred example, the detection is auxiliary detection.
In another preferred embodiment, the assay is a non-therapeutic and non-diagnostic assay.
In another preferred embodiment, the sample comprises cell lysate, tissue lysate, plasma, serum, whole blood, urine, or sputum.
In another preferred embodiment, the sample to be tested contains a tissue cell protein, such as HER2 protein.
It is understood that within the scope of the present invention, the above-mentioned technical features of the present invention and those specifically described below (e.g. in the examples) can be combined with each other to constitute new or preferred technical solutions. Not to be reiterated herein, but to the extent of space.
Drawings
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted; the same or similar reference numerals correspond to the same or similar parts; the terms describing positional relationships in the drawings are for illustrative purposes only and are not to be construed as limiting the patent.
FIG. 1 is a schematic view of the structural formula of the test piece of example 1.
Wherein the various numbers in the figures represent:
1 is a substrate, 2 is a sample adding area, 3 is a colloidal gold area, 4 is a water absorption area and 5 is a detection point.
Detailed Description
The present inventors have made extensive and intensive studies and, as a result of extensive screening and investigation, have unexpectedly found a test strip capable of rapidly and quantitatively detecting a tissue cell protein, in which test strips the detection spots on the protein detection region exhibit a specific arrangement. The detection sheet can carry out rapid and accurate quantitative detection on the tissue cell protein. On this basis, the inventors have completed the present invention.
Term(s) for
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
As used herein, the terms "comprises," "comprising," "includes," "including," and "including" are used interchangeably and include not only closed-form definitions, but also semi-closed and open-form definitions. In other words, the term includes "consisting of … …", "consisting essentially of … …".
As used herein, the term "HER 2 protein" is used interchangeably with "proto-oncogene human epidermal growth factor receptor-2 (HER 2)", and HER2 refers to a target protein for targeted therapy of breast cancer, and HER 2-positive (over-expressed or amplified) breast cancer has a good therapeutic effect on targeted drugs such as herceptin targeting HER2, and the treatment mode is greatly different from other types of breast cancer.
For convenience of explanation, the present invention will be further described with reference to fig. 1, in which the present invention is to be understood not to be limited by the accompanying drawings.
Test piece
The utility model provides a test piece for detecting histocyte protein, the test piece can carry out quick qualitative and quantitative determination to the histocyte protein level, especially carry out quick qualitative and quantitative determination with the cell protein level that tumour cell is relevant.
The test strip of the utility model can also be called as a reagent strip or a test card, etc
Typically, the test piece comprises a substrate 1, and a sample adding area 2, a colloidal gold area 3, a protein detection area and a water absorption area 4 which are arranged on the substrate in sequence from one end of the substrate to the other end of the substrate;
the protein detection area comprises a plurality of detection points 5, the detection points are arranged in a plurality of rows, the circle centers of the detection points in each row are mutually connected to form a straight line, and the straight line is vertical to the flowing advancing direction of a sample to be detected;
the spots on the protein detection zone are presented in an alternating array.
In the present invention, it should be understood that the alternating array means that the detection points in two adjacent rows are arranged alternately. In other words, the alternating array means that a gap between two adjacent detection points of one column corresponds to one detection point of the other adjacent column.
In the present invention, the substrate may also be referred to as a substrate, a backing, or the like, and serves as a support.
The utility model discloses in, the sample that awaits measuring flows the direction of advance for the direction that flows from the application of sample district to the district that absorbs water, and with every straight line perpendicular that interconnect formed between the centre of a circle of inspection point.
Sample addition zone
In the test piece, the sample adding region is used for adding a sample to be tested. The sample addition zone can comprise a sample addition pad. Preferably, the sample addition member is a fibrous membrane.
In another preferred embodiment, the sample to be tested may include cell lysate, tissue lysate, plasma, serum, whole blood, urine or sputum. Preferably, the test sample contains a tissue cell protein, such as HER2 protein.
Colloidal region
In the test strip, the colloidal gold region may contain a gold reaction pad.
The colloidal gold region may be loaded with one or more complexes selected from the group consisting of: colloidal gold antibody complex, colloidal silver antibody complex.
In another preferred embodiment, the colloidal gold-antibody complex is formed by coupling colloidal gold with an antibody selected from the group consisting of: a monoclonal antibody to be detected for a tissue cell protein, a polyclonal antibody to be detected for a tissue cell protein, or a combination thereof.
In another preferred embodiment, the colloidal silver antibody complex is formed by coupling colloidal silver with an antibody selected from the group consisting of: a monoclonal antibody to be detected for a tissue cell protein, a polyclonal antibody to be detected for a tissue cell protein, or a combination thereof.
In another preferred embodiment, the tissue cell protein to be detected is HER2 protein.
In another preferred embodiment, the colloidal gold region further supports IgG colloidal gold complex.
In another preferred example, the colloidal gold region is provided with glass fibers, and the composite is coated on the glass fibers.
Protein detection zone
The specific structure of the protein detection zone in the test strip can enable the test strip to carry out rapid qualitative and quantitative detection on the protein level of the histiocyte,
in the present invention, the shape of the detection point is not particularly limited as long as the object of the present invention is satisfied, and a preferable shape of the detection point includes (but is not limited to): circular, square, or a combination thereof. More preferably, the shape of the detection point is a circle.
In this context, it should be understood that if the shape of the detection point is not a circle, when defining features such as the inner diameter of the detection point, the detection point needs to be simulated as a circle, and the specific simulation process is as follows: the two farthest points in the shape of the detection points were set as diameters, and the simulation was circular.
The utility model discloses in, the internal diameter of check point refers to the diameter of check point, and if the check point is circular, the internal diameter refers to the diameter of circular check point.
In a preferred embodiment of the present invention, the inner diameter of each detection point is 0.5-1.2mm, preferably 0.6-1.0mm, more preferably 0.7-0.9 mm.
In another preferred embodiment, the distance between the centers of two adjacent detecting points in each row is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred embodiment, the distance between the centers of the two adjacent detecting points in one row and the detecting point in the other adjacent row corresponding to the gap therebetween is 1.3-2.1mm, preferably 1.4-1.8mm, and more preferably 1.6-1.8 mm.
In another preferred embodiment, the detecting points are arranged in n +1 columns, where n is 3-30, preferably 3-20, more preferably 3-15, and even more preferably 5-12, and n is a positive integer (e.g., 1, 2, 3, 4, 9, 15, or 30, etc.).
In another preferred embodiment, the vertical distance between two adjacent columns is 1.0-1.8mm, preferably 1.2-1.6mm, more preferably 1.3-1.5 mm. The utility model discloses in, the vertical distance between adjacent two indicates the vertical distance between the straight line that the centre of a circle interconnect of the check point of adjacent two formed. In other words, the "vertical distance between two adjacent columns" refers to a vertical distance between a straight line (L1) formed by connecting the centers of the detection points in one column to each other and a straight line (L2) formed by connecting the centers of the detection points in the other adjacent column to each other, that is, a vertical distance between the straight line (L1) and the straight line (L2).
Quality control region
The test piece of the utility model can also comprise a quality control area positioned on the substrate. The quality control region may be provided with a quality control line, preferably perpendicular to the flow advancing direction. Preferably, the quality control area is positioned between the protein detection area and the water absorption area.
A quality control line can be used to indicate whether a detection process (e.g., a chromatography process) is normal. For example, in the test strip of the present invention, the sample application region is coated with a colloidal gold complex of IgG, the quality control line is coated with an antibody of the IgG, and the IgG and the antibody of the IgG perform quality control, indicating whether a certain detection process (chromatography process) is normal; that is, if the IgG can normally reach the quality control line by chromatography, the antibody of the IgG on the quality control line binds to the IgG, thereby developing color; if the test card is failed or damaged, the IgG can not normally reach the quality control line, and the color can not be developed.
Accordingly, the skilled person can select the IgG to be used in the present invention at his or her discretion. In particular embodiments, the IgG includes, but is not limited to: rabbit IgG, horse IgG, mouse IgG, and the like. Accordingly, the skilled person can select the IgG antibody for use in the present invention by himself, as long as the IgG antibody is an antibody to said IgG. In particular embodiments, the IgG antibodies include, but are not limited to: goat anti-rabbit IgG, goat anti-horse IgG, and the like.
The utility model discloses in, add appearance district, colloidal gold district, albumen detection district and water absorption zone are located the substrate.
Water absorption zone
In the utility model discloses, the district that absorbs water can include absorbent paper. The water absorption area drives a sample to be detected to flow through the colloidal gold area and the protein detection area from the sample adding area in sequence under the action of water absorption, and the sample reaches the water absorption area, so that smooth detection is guaranteed.
The utility model discloses a main advantage includes:
1. the test piece can carry out accurate qualitative and quantitative determination to histiocyte protein (like cancer cell histones HER2) etc. fast, and sensitivity is high, improves disease diagnosis and treatment greatly.
2. The prior art mainly detects tissue cell proteins (such as cancer cell tissue protein HER2) by detection methods such as high performance liquid chromatography-tandem mass spectrometry, high performance liquid chromatography, biochip technology and the like, but the technologies have obvious defects, such as complex operation, expensive equipment, time consumption, unsuitability for on-site detection and the like. In addition, the method for detecting the tissue cell protein (such as cancer cell tissue protein HER2) in the prior art has higher requirements on instruments, equipment and operators and longer required time, thereby causing higher detection cost.
The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers. Unless otherwise indicated, percentages and parts are by weight.
Example 1
Test piece
Embodiment 1 provides a test piece, which comprises a substrate, wherein a sample adding pad, a colloidal gold region, a protein detection region and absorbent paper are sequentially arranged on the substrate from one end of the substrate to the other end of the substrate, and a sample to be tested flows through the colloidal gold region, the protein detection region and the absorbent paper sequentially after being added to the sample adding pad;
a colloidal gold region: the colloidal gold region is loaded with a colloidal gold complex of HER2 antibody, and the colloidal gold complex is coated on glass fiber;
protein detection area: the protein detection area contains a plurality of detection points, and the detection points are loaded with a mouse antibody of anti-HER 2 and are fixed on a nitrocellulose membrane; all the detection points are identical in shape and are circular, the detection points are arranged in multiple rows, the circle centers of each row of detection points are connected with each other to form a straight line, the straight line is perpendicular to the flowing advancing direction of a sample to be detected, the detection points on the protein detection area are in an alternative array, the circle center distance between every two adjacent detection points in each row is identical, the straight lines formed by the mutual connection between the circle centers of the detection points in each row are parallel to each other, the inner diameter d1 of each detection point is 0.8mm, the distance between the circle centers of the adjacent detection points in each row is d 2mm, the distance between the circle centers of two adjacent detection points in one row and one detection point in the other row corresponding to the gap between the two adjacent detection points in one row is d3 and d4 respectively, d3 is 1.66mm, and d4 is.
Example 2
Quantitative detection of HER2 protein
The test piece of example 1 was used to test HER2 protein standard solutions of different concentrations, the specific test method was as follows: HER2 protein standards were diluted proportionally to 4ng/ml, 20ng/ml, 100ng/ml, 500ng/ml and 2500ng/ml in phosphate buffer. Then 100 mul of HER2 protein standard dilution liquid with different concentrations is added into the sample adding area of the test piece, and after 5 minutes, the protein detection point of the test piece of the HER2 protein standard solution with different concentrations shows positive color development. The number of the positive color development detection points of each test piece and the absorbance value thereof are respectively counted by an absorbance scanning detector, and the total absorbance value (Ab525nm) is calculated, wherein the total absorbance value is the sum of the absorbance values of all the detection points to the light with the wavelength of 525nm, and is specifically shown in Table 1.
TABLE 1 correspondence of HER2 concentrations to total absorbance
| HER2 concentration (C) | 4ng/ml | 20ng/ml | 100ng/ml | 500ng/ml | 2500ng/ml |
| Total Absorbance (Ab) | 5 | 17 | 67 | 438 | 1709 |
A is 0.68C +22.10, correlation coefficient R2＝0.9966
As can be seen from table 1, the test strip of example 1 enables accurate quantitative determination of HER2 protein standard solution, and therefore, the test strip of example 1 can be used for quantitative determination of HER2 protein concentration, and the accuracy of the quantitative result is high.
Example 3
Example 3 the HER2 protein expression in breast cancer was measured in 5 subjects by immunohistochemistry, western blotting, and the test strip of example 1, respectively, and the accuracy of HER2 protein expression in breast cancer was compared to the test strip of example 1, wherein immunohistochemistry is a clinically routine test method and western blotting is a third gold standard, and HER2 protein (labeled 1, 2, 3, 4, and 5, respectively) in breast cancer tissues of 5 subjects was used for the immunohistochemistry, western blotting, and the test strip of example 1, and samples of the same number were derived from the breast cancer tissues of the same subjects.
Immunohistochemical detection
Fixing the tumor specimen by formaldehyde after operation and embedding paraffin in parallel; and (3) after slicing, dewaxing, hydrating, permeating and sealing, adding a mouse HER2 antibody, finally incubating with a horseradish peroxidase-labeled goat anti-mouse IgG secondary antibody, finally developing color, better shooting by a microscope, wherein the darker the color is, the higher the expression of the detected protein is. Specimens of the same tissue from different subjects were tested immunohistochemically.
Gold standard western blot detection
Tumor samples are homogenized by tissues, broken by SDS lysate to extract total protein, after the protein concentration is measured, the total protein with the same mass is taken, after heating and denaturation, the total protein is separated by 8 percent SDS-polyacrylamide gel and transferred to a nitrocellulose membrane with the diameter of 0.45 mu m, and finally, scanning imaging is carried out after incubation by a closed secondary antibody, a primary antibody and a fluorescein labeled secondary antibody. Samples of the same tissue from different subjects were examined by western blot.
Test piece detection of example 1
The test piece of example 1 was used to test the expression level of HER2 protein in breast cancer tissues of 5 subjects by the following specific test method: after 0.1g of fresh breast cancer tissue is cut into pieces, 250 microliters of protein lysate is added and completely cracked under the grinding effect, and then the pieces are heated at 95 ℃ for 5 minutes and rapidly cooled on ice. Then 50 microliters of the tissue lysate was added to the sample application zone of the test strip, and after 5 minutes, HER2 protein expression was detected in different subjects.
As can be seen from the immunohistochemical method, the Western blot detection method and the detection results of the test strip in example 1, the detection results of the test strip in example 1 were different from those of the conventional immunohistochemical detection results, but were consistent with the results of the third gold standard (Western blot detection), the detection results of the specimen 4 and the specimen 5 show that the immunohistochemistry presents a trend obviously inconsistent with the result of the gold standard western blotting detection, however, the test piece of example 1 showed a high level of consistency with the results of Western blotting, thus indicating that the test piece of example 1 is higher in detection accuracy than the conventional immunohistochemical detection, as can be seen, the values of the test strip of example 1 between the different samples are more consistent with the results of the Western blotting, thus, it was demonstrated that the test piece of example 1 had higher detection accuracy than the conventional immunohistochemical detection.
Comparative example 1
Comparative example 1 provides a test piece, which is different from example 1 in that: the spots on the protein detection zone are presented in parallel arrays.
According to the method for quantitatively detecting HER2 protein in example 2, the results of the test pieces in example 1 and the test pieces in comparative example 1 on HER2 protein standard solutions with the same concentration are as follows: the number of the detection points of the positive chromogenic protein of the test piece in the comparative example 1 is obviously less than that of the detection points of the positive chromogenic protein of the test piece in the example 1, the more the number of the chromogenic detection points is, the more excellent the accuracy and the sensitivity of the determination structure is, the more the test piece in the example 1 is obviously superior to the test piece in the comparative example 1, the total absorbance values obtained by an absorbance scanning detector are 2170 and 2930 respectively (the greater the absorbance value is, the higher the accuracy and the sensitivity is), and the total absorbance value is the sum of the absorbance values of all the detection points to light with wavelength.
All documents mentioned in this application are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (8)
1. A test piece for rapidly and quantitatively detecting tissue cell protein is characterized by comprising a substrate (1), wherein a sample adding area (2), a colloidal gold area (3), a protein detection area and a water absorption area (4) which are positioned on the substrate are sequentially arranged from one end of the substrate to the other end of the substrate;
the protein detection area comprises a plurality of detection points (5), the detection points are arranged in a plurality of rows, the circle centers of the detection points in each row are mutually connected to form a straight line, and the straight line is vertical to the flowing and advancing direction of a sample to be detected;
the spots on the protein detection zone are presented in an alternating array.
2. The test strip for rapid quantitative determination of tissue cell proteins according to claim 1, wherein the shape of the detection spots is circular.
3. The test strip for rapid quantitative determination of tissue cell proteins according to claim 1, wherein the spots are arranged in n +1 columns, where n is 3 to 30 and n is a positive integer.
4. The test strip for rapid quantitative determination of tissue cell proteins according to claim 1, wherein the shape of each of the spots is the same.
5. The test strip for rapid quantitative determination of tissue cell proteins according to claim 1, wherein the inner diameter of each spot is 0.5 to 1.2 mm.
6. The test strip for rapid quantitative determination of tissue cell proteins according to claim 1, wherein the distance between the centers of two adjacent spots in each row is 1.3-2.1 mm.
7. The strip according to claim 1, wherein the distance between the centers of circles of two spots in one row corresponding to the gap between the two spots in the other row is 1.3-2.1 mm.
8. The test strip for rapid quantitative detection of tissue cell proteins according to claim 1, wherein the test strip further comprises a quality control region on the substrate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020233533A1 (en) * | 2019-05-17 | 2020-11-26 | Mei Jun | Test piece for rapid quantitative detection of tissue cell proteins |
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| WO2020233533A1 (en) * | 2019-05-17 | 2020-11-26 | Mei Jun | Test piece for rapid quantitative detection of tissue cell proteins |
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