CN210226480U - Actinidia arguta tissue culture device - Google Patents

Actinidia arguta tissue culture device Download PDF

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Publication number
CN210226480U
CN210226480U CN201921155577.4U CN201921155577U CN210226480U CN 210226480 U CN210226480 U CN 210226480U CN 201921155577 U CN201921155577 U CN 201921155577U CN 210226480 U CN210226480 U CN 210226480U
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CN
China
Prior art keywords
connecting pipe
incubator
water tank
tissue culture
actinidia arguta
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201921155577.4U
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Chinese (zh)
Inventor
Lijing Chen
陈丽静
Li Zhang
张丽
Gang Chen
陈罡
Yubo Wang
王禹博
Shaokun Sun
孙少坤
Jun Sun
孙俊
Ying Su
苏瑛
Zhirong Huang
黄智荣
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Shenyang Agricultural University
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Shenyang Agricultural University
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Priority to CN201921155577.4U priority Critical patent/CN210226480U/en
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Publication of CN210226480U publication Critical patent/CN210226480U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model discloses a actinidia arguta tissue culture device, which comprises an incubator and a bottom box, wherein three containing plates are arranged in the incubator in a sliding manner from top to bottom, the inner wall of the incubator at the upper ends of the three containing plates is provided with a LED lamp and an electric heating plate, the input end and the output end of an air pump in the bottom box are respectively connected with one ends of a first connecting pipe and a second connecting pipe, the other end of the first connecting pipe penetrates out of the bottom box, the other end of the second connecting pipe is inserted into the bottom in the water tank, and a third connecting pipe arranged at the upper end of the water tank is inserted into the incubator; compared with the prior art, the beneficial effects of the utility model are that: when in use, the culture medium is placed on the containing plate, and the illumination time, the illumination intensity and the temperature in the incubator can be controlled through the LED lamp and the electric hot plate; gas is injected into the water tank through the gas pump, and the gas flow is discharged into the incubator from the third connecting pipe after passing through the water in the water tank, so that the gas mobility in the incubator can be improved, and the humidity in the incubator can be improved.

Description

Actinidia arguta tissue culture device
Technical Field
The utility model relates to a actinidia arguta tissue culture technical field specifically is an actinidia arguta tissue culture device.
Background
The tissue culture method of actinidia arguta mainly comprises the steps of explant sterilization, inoculation, subculture proliferation and rooting, wherein when the explant is sterilized, the tender shoots extracted by water culture are mainly cleaned by a washing powder solution by adopting a conventional method, and then sterilized by 0.1% mercuric chloride for five minutes in an ultra-static workbench, and then immersed by sterile water for forty minutes; during inoculation, subculture proliferation and rooting, actinidia arguta tissues are cultured in culture media with different components for a period of time at a certain culture temperature, culture humidity, oxygen concentration, illumination period and illumination intensity, so that the culture quality of the actinidia arguta tissues is improved, and the growth rate and survival rate of the actinidia arguta tissues are improved;
however, in the current tissue culture method of actinidia arguta, a culture medium of actinidia arguta tissues is mainly placed indoors for culture, the illumination period and the illumination intensity are controlled through indoor light, but the culture temperature, the culture humidity and the oxygen concentration cannot be accurately controlled, and therefore, the actinidia arguta tissue culture device is provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a actinidia arguta tissue culture device to solve the problem of proposing among the above-mentioned background art.
The utility model provides a soft jujube kiwi fruit tissue culture device, includes incubator and under casing, the front end of incubator rotates and is provided with the door plant, is provided with transparent observation window on the door plant, slide from last to sliding down in the incubator and be provided with three and hold the board, follow supreme three space that holds board upper end crescent down to satisfy the growth demand of different growth phase soft jujube kiwi fruit tissues, it is three to be located all be provided with the LED lamp on holding the incubator both sides inner wall of board upper end, it is three all be provided with the electric plate on the incubator rear side wall that holds board upper end, the electric plate is used for heating the air in the incubator, LED lamp and electric plate all are connected with the integrated switch electricity, through the work of integrated switch control LED lamp and electric plate, top in the incubator still is provided with temperature sensor, humidity transducer and oxygen sensor, temperature sensor, The humidity sensor and the oxygen sensor are both electrically connected with the display screen, and the temperature, humidity and oxygen concentration in the incubator, which are detected by the temperature sensor, the humidity sensor and the oxygen sensor, can be displayed on the display screen; a first through hole is formed in the upper end of the incubator, a filter screen is arranged in the first through hole, and the filter screen prevents dust from entering the incubator from the first through hole;
the incubator is characterized in that the incubator is positioned at the lower end of the incubator, an air pump is arranged in the incubator, the input end of the air pump is connected with one end of a first connecting pipe, the other end of the first connecting pipe penetrates out of the incubator, the output end of the air pump is connected with one end of a second connecting pipe, the other end of the second connecting pipe is inserted into the bottom of the water tank, water is placed in the water tank, a third connecting pipe is further installed at the upper end of the water tank, the upper end of the third connecting pipe is inserted into the incubator, a second switch valve is arranged on the first connecting pipe, a fourth switch valve is arranged at one end, close to the water tank, of the second connecting pipe, and switches of the second switch valve and the fourth switch valve are arranged on the;
one end of the second connecting pipe close to the air pump is connected with one end of a fourth connecting pipe and one end of a fifth connecting pipe, the other end of the fourth connecting pipe penetrates out of the bottom box, the other end of the fifth connecting pipe is connected with the third connecting pipe, a third switch valve is arranged on the fourth connecting pipe, a first switch valve is arranged on the fifth connecting pipe, and the first switch valve and the third switch valve are arranged on the outer wall of the bottom box.
Preferably, the upper end of water tank still is connected with the one end of sixth connecting pipe, the other end of sixth connecting pipe alternates out the under casing, still be provided with the fifth ooff valve on the sixth connecting pipe, the user can add water to the water tank in through the sixth connecting pipe to through fifth ooff valve control switch, the bottom of water tank is connected with the one end of seventh connecting pipe, the lower extreme of under casing is worn out to the other end of seventh connecting pipe, still be provided with the sixth ooff valve on the seventh connecting pipe, the user can get rid of the water in the water tank through the seventh connecting pipe to through sixth ooff valve control switch.
Preferably, the slide rail is installed to the both sides of holding the board, the slide rail slides and sets up in the slide, the slide is installed on the lateral wall in the incubator, holds the board and can slide in the slide through the slide rail.
Preferably, the containing plate is provided with a second through hole; the second through hole is convenient for the air current to pass through the containing plate from bottom to top.
Preferably, the display screen and the integrated switch are both arranged on the outer side surface of the incubator, so that the display screen can be observed conveniently, and the integrated switch can be used conveniently.
Preferably, the door plate is provided with a handle, and the door plate is convenient to control to be opened and closed through the handle.
Preferably, the universal wheels are installed at the lower end of the bottom box and are self-locking universal wheels, so that the incubator and the bottom box can be conveniently moved.
Preferably, the lower end of the air pump is provided with a rubber pad, and the rubber pad can buffer vibration generated during the working of the air pump.
Compared with the prior art, the beneficial effects of the utility model are that: three containing plates are arranged in the incubator in a sliding mode from top to bottom, the inner walls of the incubator at the upper ends of the three containing plates are provided with LED lamps and electric heating plates, three culture mediums for inoculation, subculture multiplication and rooting are respectively placed on the three containing plates from bottom to top when in use, the containing plates can be pulled out of the incubator to be carefully observed when the culture condition is observed, and the illumination time, the illumination intensity and the temperature in the incubator can be controlled through the LED lamps and the electric heating plates;
the input end and the output end of an air pump in the bottom box are respectively connected with one end of a first connecting pipe and one end of a second connecting pipe, the other end of the first connecting pipe penetrates out of the bottom box, the other end of the second connecting pipe is inserted into the bottom in the water tank, a third connecting pipe arranged at the upper end of the water tank is inserted into the incubator, and one ends of the first connecting pipe and the second connecting pipe, which are close to the water tank, are respectively provided with a second switch valve and a fourth switch valve; a user can inject gas into the water tank through the gas pump, and the gas flow is discharged into the incubator from the third connecting pipe after passing through the water in the water tank, so that on one hand, the gas fluidity in the incubator can be improved, the growth and development of actinidia arguta tissues are promoted, and on the other hand, the humidity in the incubator can be improved after the gas flow passes through the water tank;
one end of a fourth connecting pipe and one end of a fifth connecting pipe are connected to one end, close to the air pump, of the second connecting pipe, the other end of the fourth connecting pipe penetrates out of the bottom box, the other end of the fifth connecting pipe is connected with the third connecting pipe, and a third switch valve and a first switch valve are arranged on the fourth connecting pipe and the fifth connecting pipe respectively; the fifth connecting pipe plays a role of connecting the water tank in parallel, when the humidity in the incubator is high, the second connecting pipe is closed by closing the fourth switch valve, and then the fifth connecting pipe is communicated by opening the first switch valve, so that air pumped by the air pump directly enters the incubator through the fifth connecting pipe without passing through the water tank; the user can also communicate with an external oxygen machine through a fourth connecting pipe, so that oxygen is discharged into the water tank through the fourth connecting pipe and then enters the incubator; or directly get into the incubator through the fifth connecting pipe in, realize adjusting the purpose of the interior oxygen concentration of incubator, promote the growth of actinidia arguta tissue.
Drawings
Fig. 1 is a perspective view of the present invention;
fig. 2 is a structural sectional view of the present invention.
In the figure: 1. the incubator comprises an incubator 101, a first through hole, 2, a bottom case, 3, a containing plate, 301, a second through hole, 4, a slide way, 5, a slide rail, 6, an LED lamp, 7, an electric heating plate, 8, a temperature sensor, 9, a humidity sensor, 10, an oxygen sensor, 11, a filter screen, 12, a display screen, 13, an integrated switch, 14, a universal wheel, 15, an air pump, 16, a first connecting pipe, 17, a second connecting pipe, 18, a third connecting pipe, 19, a fourth connecting pipe, 20, a fifth connecting pipe, 21, a sixth connecting pipe, 22, a first switch valve, 23, a second switch valve, 24, a third switch valve, 25, a fourth switch valve, 26, a seventh connecting pipe, 27, a fifth switch valve, 28, an eighth connecting pipe, 29, a sixth switch valve, 30, a rubber pad, 31, a door panel, 32 and a handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: a actinidia arguta tissue culture device comprises a culture box 1 and a bottom box 2, wherein a door plate 31 is rotatably arranged at the front end of the culture box 1, a transparent observation window is arranged on a door plate 21, a handle 32 is arranged on the door plate 31, and the door plate 31 can be conveniently controlled to be opened or closed through the handle 32; three containing plates 3 are arranged in the incubator 1 in a sliding mode from top to bottom, sliding rails 5 are arranged on two sides of each containing plate 3, the sliding rails 5 are arranged in sliding ways 4 in a sliding mode, the sliding ways 4 are arranged on the side walls in the incubator 1, and the containing plates 3 can slide in the sliding ways 4 through the sliding rails 5; the containing plate 3 is provided with a second through hole 301, and the second through hole 301 facilitates airflow to pass through the containing plate 3 from bottom to top; the space of the upper ends of three containing plates 3 from bottom to top is gradually increased to meet the growth requirements of actinidia arguta tissues in different growth stages, the inner walls of two sides of an incubator 1 at the upper end of each containing plate 3 are provided with LED lamps 6, the rear side wall of the incubator 1 at the upper end of each containing plate 3 is provided with an electric heating plate 7, each electric heating plate 7 is used for heating air in the incubator 1, each LED lamp 6 and each electric heating plate 7 are electrically connected with an integrated switch 13, the work of each LED lamp 6 and each electric heating plate 7 is controlled through the integrated switch 13, three culture mediums for inoculation, subculture multiplication and rooting are respectively placed on the three containing plates 3 from bottom to top when the three containing plates are used, the containing plates 3 can be pulled out of the incubator 1 to be carefully observed when the culture conditions are observed, and the illumination time in the incubator 1 can be controlled through the LED lamps 6 and the electric heating plates 7, Illumination intensity and temperature; the top in the incubator 1 is also provided with a temperature sensor 8, a humidity sensor 9 and an oxygen sensor 10, the temperature sensor 8, the humidity sensor 9 and the oxygen sensor 10 are all electrically connected with a display screen 12, the temperature, the humidity and the oxygen concentration in the incubator 1 detected by the temperature sensor 8, the humidity sensor 9 and the oxygen sensor 10 can be displayed on the display screen 12, and the display screen 12 and the integrated switch 13 are both arranged on the outer side surface of the incubator 1, so that the display screen 12 can be observed conveniently and the integrated switch 13 can be used conveniently; first through-hole 101 has been seted up to the upper end of incubator 1, be provided with filter screen 11 in the first through-hole 101, filter screen 11 prevents that the dust from getting into incubator 1 from first through-hole 101.
The bottom box 2 is positioned at the lower end of the incubator 1, the universal wheels 14 are mounted at the lower end of the bottom box 2, and the universal wheels 14 are self-locking universal wheels, so that the incubator 1 and the bottom box 2 can move conveniently; an air pump 15 is arranged in the bottom box 2, a rubber pad 30 is arranged at the lower end of the air pump 15, and the rubber pad 30 can buffer vibration generated when the air pump 15 works; the input end of the air pump 15 is connected with one end of a first connecting pipe 16, the other end of the first connecting pipe 16 penetrates out of the bottom box 2, the output end of the air pump 15 is connected with one end of a second connecting pipe 17, the other end of the second connecting pipe 17 is inserted into the bottom of the water tank 18, water is placed in the water tank 18, a third connecting pipe 19 is further installed at the upper end of the water tank 18, the upper end of the third connecting pipe 19 is inserted into the incubator 1, a second switch valve 23 is arranged on the first connecting pipe 16, a fourth switch valve 25 is arranged at one end, close to the water tank 18, of the second connecting pipe 17, and switches of the second switch valve 23 and the fourth switch valve 25 are arranged on the outer wall of the bottom box 2; the user can be through air pump 15 gas injection in to water tank 18, and the air current can improve the gas mobility in incubator 1 in discharging from third connecting pipe 19 behind the water in the water tank 18 on the one hand, promotes the growth and development of actinidia arguta tissue, and on the other hand air current can improve the humidity in incubator 1 after water tank 18.
One end of the second connecting pipe 17, which is close to the air pump 15, is connected with one end of a fourth connecting pipe 20 and one end of a fifth connecting pipe 21, the other end of the fourth connecting pipe 20 penetrates out of the bottom box 2, the other end of the fifth connecting pipe 21 is connected with a third connecting pipe 19, a third on-off valve 24 is arranged on the fourth connecting pipe 20, a first on-off valve 22 is arranged on the fifth connecting pipe 21, and switches of the first on-off valve 22 and the third on-off valve 24 are arranged on the outer wall of the bottom box 2; the fifth connecting pipe 21 plays a role of connecting the water tank 18 in parallel, when the humidity in the incubator 1 is high, the second connecting pipe 17 is closed by closing the fourth switch valve 20, and then the fifth connecting pipe 21 is communicated by opening the first switch valve 22, so that the air pumped by the air pump 15 directly enters the incubator 1 through the fifth connecting pipe 21 without passing through the water tank 18; the user can also communicate with an external oxygen machine through the fourth connecting pipe 20, so that oxygen is discharged into the water tank 18 through the fourth connecting pipe 20 and then enters the incubator 1; or directly get into incubator 1 through fifth connecting pipe 21 in, realize adjusting the purpose of incubator 1 interior oxygen concentration, promote the growth of actinidia arguta tissue.
The upper end of water tank 18 still is connected with the one end of sixth connecting pipe 26, the other end of sixth connecting pipe 26 has alternated out under casing 2, still be provided with fifth ooff valve 27 on the sixth connecting pipe 26, the user can add water to water tank 18 through sixth connecting pipe 26 to through fifth ooff valve 27 control switch, the bottom of water tank 18 is connected with the one end of seventh connecting pipe 28, the lower extreme of under casing 2 is worn out to the other end of seventh connecting pipe 28, still be provided with sixth ooff valve 29 on the seventh connecting pipe 28, the user can get rid of the water in the water tank 18 through seventh connecting pipe 28 to through sixth ooff valve 29 control switch.
The working principle is as follows: when the utility model is used, three culture mediums for inoculation, subculture multiplication and rooting are respectively placed on the three containing plates 3 from bottom to top, and the illumination time, the illumination intensity and the temperature in the incubator 1 can be controlled through the LED lamp 6 and the electric heating plate 7;
a user injects gas into the water tank 18 through the gas pump 15, and the gas flow is discharged into the incubator 1 from the third connecting pipe 19 after passing through the water in the water tank 18, so that on one hand, the gas fluidity in the incubator 1 can be improved, the growth and development of actinidia arguta tissues can be promoted, and on the other hand, the humidity in the incubator 1 can be improved after the gas flow passes through the water tank 18; when the humidity in the incubator 1 is high, the second connecting pipe 17 is closed by closing the fourth switch valve 20, and the fifth connecting pipe 21 is communicated by opening the first switch valve 22, so that the air pumped by the air pump 15 directly enters the incubator 1 through the fifth connecting pipe 21 without passing through the water tank 18;
the user can also communicate with an external oxygen machine through the fourth connecting pipe 20, so that oxygen is discharged into the water tank 18 through the fourth connecting pipe 20 and then enters the incubator 1; or directly get into incubator 1 through fifth connecting pipe 21 in, realize adjusting the purpose of incubator 1 interior oxygen concentration, promote the growth of actinidia arguta tissue.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. The utility model provides a actinidia arguta tissue culture device, includes incubator and under casing, its characterized in that: the front end of the incubator is rotatably provided with a door plate, three containing plates are arranged in the incubator in a sliding manner from top to bottom, LED lamps are arranged on the inner walls of two sides of the incubator at the upper ends of the three containing plates, electric heating plates are arranged on the rear side walls of the incubator at the upper ends of the three containing plates, the LED lamps and the electric heating plates are electrically connected with an integrated switch, the top in the incubator is also provided with a temperature sensor, a humidity sensor and an oxygen sensor, and the temperature sensor, the humidity sensor and the oxygen sensor are electrically connected with a display screen; a first through hole is formed in the upper end of the incubator, and a filter screen is arranged in the first through hole;
the incubator is positioned at the lower end of the incubator, an air pump is arranged in the incubator, the input end of the air pump is connected with one end of a first connecting pipe, the other end of the first connecting pipe penetrates out of the incubator, the output end of the air pump is connected with one end of a second connecting pipe, the other end of the second connecting pipe is inserted into the bottom of the water tank, a third connecting pipe is further installed at the upper end of the water tank, the upper end of the third connecting pipe is inserted into the incubator, a second switch valve is arranged on the first connecting pipe, and a fourth switch valve is arranged at one end, close to the water tank, of the second connecting pipe;
one end of the second connecting pipe, which is close to the air pump, is connected with one end of a fourth connecting pipe and one end of a fifth connecting pipe, the other end of the fourth connecting pipe penetrates out of the bottom box, the other end of the fifth connecting pipe is connected with the third connecting pipe, a third switch valve is arranged on the fourth connecting pipe, and a first switch valve is arranged on the fifth connecting pipe.
2. The actinidia arguta tissue culture device of claim 1, wherein: the upper end of water tank still is connected with the one end of sixth connecting pipe, the other end of sixth connecting pipe alternates out the under casing, still be provided with the fifth ooff valve on the sixth connecting pipe, the bottom of water tank is connected with the one end of seventh connecting pipe, the lower extreme of under casing is worn out to the other end of seventh connecting pipe, still be provided with the sixth ooff valve on the seventh connecting pipe.
3. The actinidia arguta tissue culture device of claim 1, wherein: the slide rail is installed to the both sides of holding the board, the slide rail slides and sets up in the slide, the slide is installed on the lateral wall in the incubator.
4. The actinidia arguta tissue culture apparatus of claim 1 or 3, wherein the actinidia arguta tissue culture apparatus comprises: and the containing plate is provided with a second through hole.
5. The actinidia arguta tissue culture device of claim 1, wherein: the display screen and the integrated switch are both arranged on the outer side surface of the incubator.
6. The actinidia arguta tissue culture device of claim 1, wherein: the door plate is provided with a handle.
7. The actinidia arguta tissue culture device of claim 1, wherein: the lower end of the bottom box is provided with universal wheels.
8. The actinidia arguta tissue culture device of claim 1, wherein: the lower end of the air pump is provided with a rubber pad.
CN201921155577.4U 2019-07-23 2019-07-23 Actinidia arguta tissue culture device Expired - Fee Related CN210226480U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921155577.4U CN210226480U (en) 2019-07-23 2019-07-23 Actinidia arguta tissue culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921155577.4U CN210226480U (en) 2019-07-23 2019-07-23 Actinidia arguta tissue culture device

Publications (1)

Publication Number Publication Date
CN210226480U true CN210226480U (en) 2020-04-03

Family

ID=69992436

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921155577.4U Expired - Fee Related CN210226480U (en) 2019-07-23 2019-07-23 Actinidia arguta tissue culture device

Country Status (1)

Country Link
CN (1) CN210226480U (en)

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Granted publication date: 20200403

Termination date: 20210723