CN210215335U

CN210215335U - 96 orifice plate/384 orifice plate application of sample auxiliary device

Info

Publication number: CN210215335U
Application number: CN201920926655.XU
Authority: CN
Inventors: Xiaolan Chen; 陈晓岚; Yuhui Huang; 黄玉辉; Yanhong Li; 李艳红
Original assignee: Affiliated Childrens Hospital of Soochow University
Current assignee: Affiliated Childrens Hospital of Soochow University
Priority date: 2019-06-19
Filing date: 2019-06-19
Publication date: 2020-03-31
Anticipated expiration: 2029-06-19

Abstract

The utility model discloses a 96 orifice plate 384 orifice plate application of sample auxiliary device, apron including 96 orifice plate 384 orifice plate, its characterized in that: the labeling device is connected with the cover plate of the 96-well plate/384-well plate through a connecting structure; the marking device mainly comprises a flexible pressure-sensitive color-changing film and a protective film covering the upper surface of the flexible pressure-sensitive color-changing film, and through holes are formed in the marking device corresponding to the positions of the sample adding holes of the 96-hole plate/384-hole plate. The utility model marks the color change of the corresponding hole site on the marking device, can realize single-hole marking, and can clearly identify even if the samples are not added in sequence; the device simple structure, it is easy and simple to handle, do not have the removal part when marking the device and using, effectively avoid the contamination of application of sample hole.

Description

96 orifice plate/384 orifice plate application of sample auxiliary device

Technical Field

The utility model relates to a biological assay check out test set, concretely relates to be used for the perforated plate, especially be used for assisting device of 96 orifice plates 384 orifice plate application of sample.

Background

The porous plate is a cell biology experimental device and is widely applied to experiments such as cell culture, fluorescence/chemiluminescence detection, drug screening and the like. Because the number of the sample holes of the 96-hole plate and the 384-hole plate is large and the sample holes are densely distributed, the conditions of wrong loading and missing loading are easy to occur in the loading process of an operator, and the accuracy of an experimental result is influenced. Although some automated sample loading devices and liquid handling systems have been developed to ensure the speed and accuracy of sample loading, manual sample loading is still an essential task for most laboratories. Therefore, how to ensure the accuracy of manual sample adding, label the loaded hole, avoid missing or repeated sample adding is the problem to be solved in the field.

In order to solve the above problems, some devices for assisting sample adding have appeared in recent years, so as to avoid sample adding errors in the experimental process. For example, chinese utility model patent CN207468648U discloses a 96-well plate sample adding marker, which comprises a base with an opening at the upper part, wherein the base is provided with an inner cavity capable of placing a 96-well plate, a plurality of sample adding holes are distributed on the 96-well plate in an array manner, a mark cover plate capable of moving back and forth along the longitudinal direction of the sample adding holes is arranged above the base, the longitudinal edge of the mark cover plate is transversely provided with indicator lamps aligned with each sample adding hole, one side of each indicator lamp is provided with a button switch for controlling the indicator lamps, and the button switch is connected on a circuit borrowing circuit; the button switch is connected with a main switch capable of turning off all the indicator lights; the edge of two lateral sides of the seat body is provided with a pair of slots with opposite opening directions, and the identification cover plate is inserted in the slots and can move transversely along the slots, one lateral end of the seat body has no longitudinal side wall, so that an opening end which is convenient for being placed in a 96-hole plate is formed; the pair of slots extend from the opening end of the seat body to the longitudinal side wall of the closed end of the seat body along the transverse side wall, and the longitudinal side wall of the closed end of the seat body provides limit for the slots; the identification cover plate is a transparent cover plate. Light the pilot lamp through the button on the sign apron and mark the application of sample hole of application of sample, close all pilot lamps through master switch after the application of sample hole application of sample is accomplished in a row, lateral shifting to next row application of sample hole application of sample, can avoid appearing the application of sample mistake, can not omit or repeated application of sample. The device can mark the loaded hole, but the button switch can not be controlled by the loading gun due to difficult disinfection, and the button switch needs to be pressed additionally after each loading, so the operation is inconvenient; meanwhile, the scheme can only sample according to the sequence and cannot be applied to occasions needing to add various reagents.

For convenient operation, chinese utility model CN208001392U discloses a 96 orifice plate application of sample auxiliary device, including the supporting seat that is used for placing the 96 orifice plate, set up LED lamp array and circuit control board on the supporting seat and set up the count module on the application of sample rifle, install LED lamp pearl in a plurality of slotted holes that set up on the pedestal of supporting seat respectively, the pore of 96 orifice plate matches one by one with the slotted hole of supporting seat, circuit control board integration has the controller and the radio receiver who is connected with the controller electricity, a radio transmitter is connected to the output interface electricity of count module, the controller passes through radio receiver and radio transmitter wireless connection in order to receive count module's count value signal. The device sets up red LED lamp pearl and green LED lamp pearl simultaneously in the pore plate corresponding position at 96 orifice plates, and count through setting up count module on the application of sample rifle and count, the controller generates control command according to count value in the mapping unit and the corresponding relation of slotted hole position serial number and the count value signal of received count module, control red LED lamp pearl and green LED lamp pearl are lighted in turn, the LED lamp of different colours is used to the pore plate that will the application of sample and the pore plate that adds the sample and suggests, thereby prevent that operating personnel from adding wrong sample. Because the control button setting is on the application of sample rifle, be connected through wireless mode and LED lamp array, therefore the device damage is more convenient, but, device overall structure is complicated to owing to set up circuit system, auxiliary device is difficult for cleaning and disinfecting, probably produces adverse effect to the experiment accuracy.

Disclosure of Invention

The utility model aims at providing a 96 orifice plate/384 orifice plate application of sample auxiliary device through simple structural design, realizes the assistance to 96 orifice plate/384 orifice plate application of sample, avoids the application of sample mistake, makes whole application of sample system normally disinfect simultaneously, guarantees the experiment accuracy.

In order to achieve the purpose of the invention, the technical scheme adopted by the utility model is as follows: a96-well plate/384-well plate sample adding auxiliary device comprises a cover plate of a 96-well plate/384-well plate and a marking device, wherein the marking device is arranged and is connected with the cover plate of the 96-well plate/384-well plate through a connecting structure; the marking device mainly comprises a flexible pressure-sensitive color-changing film and a protective film covering the upper surface of the flexible pressure-sensitive color-changing film, and through holes are formed in the marking device corresponding to the positions of the sample adding holes of the 96-hole plate/384-hole plate.

Among the above-mentioned technical scheme, having flexible pressure sensitive discoloration membrane among the mark device, under the slight touch of application of sample rifle head, realizing the location mark to the hole of application of sample to after the pressure is got rid of, can maintain the colour that changes and recover by the circular telegram. A flexible pressure-sensitive color-changing film capable of maintaining a changed color after removal of pressure is known in the art, and in one form, the flexible pressure-sensitive color-changing film is composed of a liquid crystal-polymer mixed material layer in the middle and upper and lower ITO film layers. For example, chinese patent CN105216390A discloses a pressure color-changing flexible film material, which uses pressure to change the crystal orientation inside the film, thereby causing color change, and uses electronic pulse impact to recover the crystal orientation inside the film. In the document disclosed by the invention, the writing board is used in the field of office and cultural and educational supplies and is manufactured. In order to be applied to 96 orifice plates application of sample supplementary, the utility model discloses it has established the protection film to add on its surface, on the one hand, realizes tolerating 75% alcohol through the protection film, makes whole device can adopt 75% alcohol disinfection, and on the other hand keeps apart hand electrostatic influence through the protection film.

And simultaneously, the utility model discloses to mark the device and pass through the cover connection of connection structure and 96 orifice plates 384 orifice plates, once use the completion after, will mark the device and remove from the apron, realize reuse through reseing. Because the reset circuit structure is separated from the marking device, the disinfection of the device cannot be influenced.

Therefore, according to a further technical scheme, the marking device restorer is arranged and electrically connected with the upper ITO film layer and the lower ITO film layer in the marking device through electric connection pieces, and an electric pulse generating mechanism for liquid crystal restoration is arranged in the marking device restorer.

In the above technical solution, the connection structure may be a glue layer which is disposed on the back side of the marking device and can be repeatedly pasted.

Alternatively, the attachment structure is formed by an attachment plate attached to the back side of the marker and a downwardly extending flange at the periphery of the attachment plate that mates with the periphery of the cover plate of the 96/384 well plate.

Or the connecting structure is that the periphery of the cover plate of the 96-orifice plate/384-orifice plate is provided with a convex edge higher than the top surface of the cover plate, the marking device is provided with a rigid layer, and the rigid layer is clamped in the convex edge of the top surface of the cover plate.

In a preferred embodiment, the protective film is a polyester film.

Because of the application of the technical scheme, compared with the prior art, the utility model has the following advantages:

1. the utility model marks the color change of the corresponding hole site on the marking device, can realize single-hole marking, and can clearly identify even if the samples are not added in sequence;

2. because the flexible pressure-sensitive color-changing film is adopted for marking, multiple marking can be realized on the same hole position through multiple points or lines in different shapes, and the flexible pressure-sensitive color-changing film can be used for marking when different samples are added in multiple times;

3. the utility model has simple structure and convenient operation, and no moving part exists when the marking device is used, thereby effectively avoiding the pollution of the sample adding hole;

4. through connection structure's setting, combine to mark device restorer, can realize the used repeatedly of device, economic environmental protection.

Drawings

FIG. 1 is a schematic top view of a cover plate of a 96-well plate;

FIG. 2 is a schematic sectional view of the first embodiment;

FIG. 3 is an enlarged partial schematic view of FIG. 2;

FIG. 4 is a schematic sectional view showing the second embodiment;

FIG. 5 is an enlarged partial schematic view of FIG. 4;

FIG. 6 is a schematic sectional view showing the third embodiment;

fig. 7 is a partially enlarged schematic view of fig. 6.

Wherein: 1. a cover plate; 2. a through hole; 3. a marking device; 4. a protective film; 5. an ITO film layer is arranged; 6. a liquid crystal-polymer mixed material layer; 7. an ITO film layer is arranged; 8. a glue layer; 9. a hard connecting plate; 10. folding edges; 11. a rigid layer; 12. a convex edge.

Detailed Description

The invention will be further described with reference to the following drawings and examples:

the first embodiment is as follows: a96-well plate sample adding auxiliary device comprises a cover plate of a 96-well plate and a marking device. The top view of the cover plate of the 96-well plate is shown in FIG. 1, and 96 through-holes 2 are arranged in the cover plate 1.

As shown in fig. 2, a marking device 3 is provided above the top surface of the cover plate 1.

Referring to fig. 3, which is a partially enlarged schematic view of fig. 2, it can be seen that the marking device 3 is a multi-layer film structure, and sequentially includes a protection film 4, an upper ITO film layer 5, a liquid crystal-polymer mixed material layer 6, and a lower ITO film layer 7 from top to bottom. Through holes are arranged on each layer corresponding to each sample adding hole of the 96-hole plate. The bottom surface of the lower ITO film layer 7 is connected with the cover plate 1 of the 96-hole plate through a glue layer 8 which can be repeatedly pasted.

In this embodiment, a marker restorer is provided, the marker restorer is electrically connected with the upper and lower ITO film layers in the marker via an electrical connection member, and an electrical pulse generation mechanism for liquid crystal restoration is provided in the marker restorer.

During the use, will mark the device and paste on the 96 orifice plates apron that correspond, slightly touch mark the device and make it discolour with the application of sample rifle head at application of sample hole side, realize the location mark to the hole of application of sample.

After the experiment is completed, the marking device can be taken down, and electric pulses are applied to the resetting device of the marking device to reset the color. This is used for the next experiment.

The structure of this embodiment can also be used for the sample adding assistance of 384-well plates, and only the through holes need to be formed at the corresponding positions of the cover plates of the corresponding 384-well plates.

Example two: referring to fig. 4, a 96-well plate sample adding auxiliary device comprises a cover plate of a 96-well plate and a marking device. The marking device mainly comprises a flexible pressure-sensitive color-changing film and a protective film covering the upper surface of the flexible pressure-sensitive color-changing film.

Referring to fig. 5, in this embodiment a rigid web 9 is provided on the back side of the labelling apparatus, the web having a downwardly extending flange 10 at its periphery, said downwardly extending flange 10 engaging the periphery of the lid of a 96-well plate. During the use, establish the device cover with marking on the apron of 96 orifice plates, realize the location and connect, can mark through pressure afterwards.

Example three: referring to fig. 4, a 96-well plate sample adding auxiliary device comprises a cover plate of a 96-well plate and a marking device. The marking device mainly comprises a flexible pressure-sensitive color-changing film and a protective film covering the upper surface of the flexible pressure-sensitive color-changing film.

In this embodiment, the periphery of the cover plate of the 96-well plate/384-well plate is provided with a flange 12 higher than the top surface of the cover plate, and the marking device is provided with a rigid layer 11, and the rigid layer 11 is clamped in the flange 12 on the top surface of the cover plate.

Claims

1. A96-well plate/384-well plate sample adding auxiliary device comprises a cover plate of a 96-well plate/384-well plate, and is characterized in that: the labeling device is connected with the cover plate of the 96-well plate/384-well plate through a connecting structure; the marking device mainly comprises a flexible pressure-sensitive color-changing film and a protective film covering the upper surface of the flexible pressure-sensitive color-changing film, and through holes are formed in the marking device corresponding to the positions of the sample adding holes of the 96-hole plate/384-hole plate.

2. The 96-well plate/384-well plate sample application auxiliary device of claim 1, wherein: the flexible pressure-sensitive color-changing film consists of a liquid crystal-polymer mixed material layer in the middle and an upper ITO film layer and a lower ITO film layer.

3. The 96-well plate/384-well plate sample application auxiliary device according to claim 2, wherein: the marking device restorer is electrically connected with the upper ITO film layer and the lower ITO film layer in the marking device through an electric connector, and an electric pulse generating mechanism for liquid crystal restoration is arranged in the marking device restorer.

4. The 96-well plate/384-well plate sample application auxiliary device of claim 1, wherein: the connecting structure is a glue layer which is arranged on the back side of the marking device and can be pasted repeatedly.

5. The 96-well plate/384-well plate sample application auxiliary device of claim 1, wherein: the attachment structure is formed by an attachment plate attached to the back side of the marker and a downwardly extending flange at the periphery of the attachment plate that mates with the periphery of the cover plate of the 96/384 well plate.

6. The 96-well plate/384-well plate sample application auxiliary device of claim 1, wherein: the connecting structure is characterized in that the periphery of the cover plate of the 96-hole plate/384-hole plate is provided with a convex edge higher than the top surface of the cover plate, the marking device is provided with a rigid layer, and the rigid layer is clamped in the convex edge on the top surface of the cover plate.

7. The 96-well plate/384-well plate sample application auxiliary device of claim 1, wherein: the protective film is a polyester film.