CN210176852U - Continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device - Google Patents
Continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device Download PDFInfo
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- CN210176852U CN210176852U CN201920580010.5U CN201920580010U CN210176852U CN 210176852 U CN210176852 U CN 210176852U CN 201920580010 U CN201920580010 U CN 201920580010U CN 210176852 U CN210176852 U CN 210176852U
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Abstract
The utility model discloses a continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device belongs to the environment-friendly device field. The reactor main body mainly comprises a reactor cover plate, a reactor outer wall, a three-phase separator, a guide cylinder, a water inlet, a sludge discharge port, a gas taking port and a methane gas bag connecting port, wherein a reaction zone is arranged in the guide cylinder, a circulation zone is formed between the guide cylinder and the reactor lower outer wall as well as between the guide cylinder and the three-phase separator, and a settling zone is arranged between the three-phase separator and the reactor upper outer wall. The water inlet system and the water outlet system are respectively connected with the reactor main body and are used for water inlet and outlet. The device of the utility model utilizes the micro-bubbles generated by the aeration system to realize the effect of air lift and muddy water mixing, provides the power of liquid phase circulation and strengthens the gas-liquid mass transfer effect. The utility model discloses a circulation aeration and continuous flow operation have realized the gaseous phase circulation, can improve the methane utilization ratio, have effectively reduced the cost of labor.
Description
Technical Field
The utility model belongs to the environmental protection device field, concretely relates to continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device.
Background
Methane is an important greenhouse gas, and the greenhouse effect caused by methane is equimolar CO226 times higher, the "contribution" to global warming is about 20%, second only to carbon dioxide. Research has shown that there are about 4X 10 years around the world per year8The ton methane is consumed by anaerobic methane oxidation carried out by microorganisms, the methane concentration in the atmosphere is effectively controlled by the anaerobic methane oxidation process catalyzed by the microorganisms, and the method has important significance for relieving the greenhouse effect which is getting worse.
The methane anaerobic oxidation microorganism is an autotrophic microorganism, the growth is slow, the multiplication time is from several weeks to several months, the slow growth rate increases the difficulty of enrichment culture of the microorganism, and the slow growth rate also becomes a key factor limiting the research and development of the field.
At present, sequencing batch reactors are mainly adopted as an enrichment device for methane anaerobic oxidation microorganisms, although the reactors are simple in equipment, higher labor cost is required to be invested, and the improvement of matrix load and hydraulic load is limited by microbial tolerance and manual operation strength respectively. Therefore, the research and development of the novel continuous flow and gas-liquid phase circulation enrichment device have great significance for accelerating the enrichment culture of the methane anaerobic oxidation microorganisms and are beneficial to promoting the engineering application of the methane anaerobic oxidation microorganisms.
Disclosure of Invention
The utility model aims at overcoming that the enrichment of current device is efficient low, the leakproofness is poor, the cost of labor is high, and load promotes not enough such as difficulty, provides a continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device.
A continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device comprises a reactor main body, a water inlet system, an aeration system and a water outlet system; the reactor main body comprises a reactor cover plate, an upper outer wall of the reactor, a lower outer wall of the reactor, a three-phase separator, a guide cylinder, an air intake port, a first methane gas bag connecting port, a dissolved oxygen probe, a pH probe, a water inlet, a bottom sludge discharge port and a bottom conical structure; the water inlet system comprises a liquid storage tank, a second methane bag connector, a water inlet pipe and a peristaltic pump; the aeration system comprises a gas circulating pump, a gas inlet, a gas outlet, a rubber pipe and an aeration head; the water outlet system comprises an overflow port, a water outlet and a water outlet weir;
the reactor main body is formed by connecting the outer wall of the upper part of the reactor with the outer wall of the lower part of the reactor; the upper part of the reactor main body is provided with a cover plate, and the cover plate is respectively provided with an air intake port, a first methane bag connecting port, a dissolved oxygen probe and a pH probe; one end of the gas taking port is sealed by a rubber plug, and the other end of the gas taking port penetrates through the cover plate and is communicated with the reactor main body; the first methane gas bag is communicated with the headspace of the inner cavity of the reactor main body through a first methane gas bag connecting port; the lower part of the cover plate is connected with a three-phase separator in a hanging way through a connecting rod, the upper part of the three-phase separator is in an inverted funnel shape, and the lower part of the three-phase separator is in a vertical cylindrical shape and has the diameter equal to that of the outer wall of the lower part of the reactor; a guide cylinder is coaxially arranged below the three-phase separator, the upper part of the guide cylinder is in an inverted funnel shape and extends into the three-phase separator, and the lower part of the guide cylinder is in a vertical cylindrical shape; the upper part of the outer wall of the upper part of the reactor is cylindrical, the diameter of the lower part of the reactor is gradually reduced to form a funnel-shaped structure, the bottom of the reactor is positioned below the three-phase separator, and an annular opening with a certain distance is reserved between the outer wall of the upper part of the reactor and the bottom of the side wall of the three-phase separator; the outer wall of the lower part of the reactor is cylindrical, and the side wall of the reactor is provided with a water inlet and a bottom sludge discharge port; the bottom of the outer wall of the lower part of the reactor is provided with a bottom conical structure, and the surface of the bottom conical structure is formed by rotating a circle of a semicircular arc with an upward opening to form an annular concave cavity; the bottom of the guide shell extends into the annular concave cavity, but the bottom of the guide shell and the annular concave cavity keep a distance; the liquid storage tank is a sealed tank body, and a second methane bag connecting port and a water inlet pipe are arranged above the tank body; the second methane bag is communicated with the headspace of the liquid storage tank through a second methane bag connecting port; one end of the water inlet pipe extends into the liquid storage tank below the liquid level, and the other end of the water inlet pipe is connected with the water inlet through the peristaltic pump; the gas circulating pump is positioned outside the reactor, two ends of the gas circulating pump are respectively connected with the gas inlet and the gas outlet, and the lower end of the gas outlet is connected with the aeration head positioned below the inner part of the guide cylinder through a rubber pipe; an overflow port is arranged on one side of the outer wall of the upper part of the reactor, the overflow port is communicated with an effluent weir, a water outlet is arranged on the side wall of the effluent weir, and the height of the water outlet is higher than that of the overflow port.
Preferably, the horizontal inclination angle of the funnel-shaped structure on the outer wall of the upper part of the reactor is 55-60 degrees, the horizontal inclination angle of the inverted funnel-shaped structure on the upper part of the guide shell is 55-60 degrees, and the inclination angles are kept consistent.
Preferably, the height-diameter ratio of the outer wall of the lower part of the reactor is 3-5: 1.
Preferably, the inner cavity of the outer wall of the lower part of the reactor is divided into two parts by the guide cylinder, a reaction zone is arranged in the guide cylinder, a circulation zone is arranged outside the guide cylinder, the height-diameter ratio of the reaction zone is 8-10: 1, and the diameter ratio of the reaction zone to the circulation zone is 1-1.5: 2.
Preferably, the width of the annular opening is 1-2 cm.
Preferably, the diameter of the semicircular arc which is rotated to form the bottom conical structure is half of the diameter of the outer wall of the lower part of the reactor.
Preferably, the dissolved oxygen probe and the pH probe both extend below the liquid level in the reactor.
Preferably, the water outlet is a pipe inclined downward.
Preferably, the first methane gas bag and the second methane gas bag are both filled with methane gas.
Preferably, the reactor body is sealed from the outside atmosphere.
Compared with the prior art, the utility model beneficial effect who has: 1) the reactor is divided into a reaction area, a circulation area and a precipitation area by using the guide cylinder and the three-phase separator, gas-liquid phase circulation is realized by the gas stripping and mixing effects of the aeration system, the gas-liquid mass transfer area is enlarged, the transfer performance is enhanced, mud and water are easily separated, the utilization rate of methane is improved, and favorable conditions are provided for enrichment culture of methane anaerobic oxidation microorganisms; 2) the device can still make the pressure difference inside and outside the reactor consistent under the condition of keeping the anaerobic environment in the reactor through the two methane gas bags, does not influence water inlet and outlet, and has the advantages of good sealing performance, simple operation and the like; 3) fresh culture solution firstly enters a circulation zone through a water inlet, then enters a reaction zone along with the action of water flow, and is rapidly mixed under the combined action of the water flow and the air flow, so that the influence of the concentration of a substrate in the fresh culture solution on methane anaerobic oxidation microorganisms is reduced, and the hydraulic load and substrate load impact resistance of the reactor can be effectively improved; 4) the continuous flow water inlet mode not only greatly reduces the labor cost, but also is more beneficial to the engineering application and popularization.
Drawings
FIG. 1 is a schematic structural diagram of a continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device.
In the figure: the device comprises a reactor main body 1, a water inlet system 2, an aeration system 3, a water outlet system 4, a reactor cover plate 5, a reactor upper outer wall 6, a reactor lower outer wall 7, a three-phase separator 8, a guide cylinder 9, an air intake 10, a first methane gas bag connecting port 11, a dissolved oxygen probe 12, a pH probe 13, a water inlet 14, a bottom sludge discharge port 15, a bottom conical structure 16, a liquid storage tank 17, a second methane gas bag connecting port 18, a water inlet pipe 19, a peristaltic pump 20, a connecting rod 21, a gas circulating pump 22, an air inlet 23, an air outlet 24, a rubber pipe 25, an aeration head 26, an overflow port 27, a water outlet 28 and a water outlet weir 29.
Detailed Description
For a further understanding of the present invention, reference should be made to the following further description and specific examples, taken in conjunction with the accompanying drawings, and it is to be understood that the description is intended to further illustrate the features and advantages of the present invention, and not to limit the scope of the invention.
As shown in figure 1, the continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device comprises a reactor main body 1, a water inlet system 2, an aeration system 3 and a water outlet system 4. The reactor main body 1 comprises a reactor cover plate 5, a reactor upper outer wall 6, a reactor lower outer wall 7, a three-phase separator 8, a guide cylinder 9, an air intake port 10, a first methane gas bag connecting port 11, a dissolved oxygen probe 12, a pH probe 13, a water inlet 14, a bottom sludge discharge port 15 and a bottom conical structure 16. The water inlet system 2 comprises a liquid storage tank 17, a second methane bag connecting port 18, a water inlet pipe 19 and a peristaltic pump 20. The aeration system 3 comprises a gas circulation pump 22, a gas inlet 23, a gas outlet 24, a rubber pipe 25 and an aeration head 26. The outlet system 4 comprises an overflow port 27, a water outlet 28 and an outlet weir 29. The specific structure of each part is described in detail below.
The reactor main body 1 is formed by connecting an upper outer wall 6 of the reactor and a lower outer wall 7 of the reactor to form a cylindrical structure, a cover plate 5 is arranged at the upper part of the reactor main body 1 for sealing, and an air intake port 10, a first methane gas bag connecting port 11, a dissolved oxygen probe 12 and a pH probe 13 are respectively arranged on the cover plate 5. The dissolved oxygen probe 12 and the pH probe 13 both extend below the liquid level in the reactor for monitoring the internal matrix environment. The gas inlet 10 is a pipe, one end of which is sealed by a rubber plug, the other end of which is communicated with the reactor main body 1 by penetrating through the cover plate 5, and the rubber plug can be removed and replaced and is used for periodically sampling and analyzing the internal headspace gas in the operation process and monitoring the operation state. The first methane gas bag is a deformable gas bag filled with a large amount of methane gas, and the gas outlet of the first methane gas bag is communicated with the headspace of the inner cavity of the reactor main body 1 through a first methane gas bag connecting port 11. Because the reactor need advance outlet water in succession, and advance outlet water process and inside operation can lead to the inside atmospheric pressure of reactor to appear undulantly, the effect of first methane gas pocket is that atmospheric pressure and outside atmospheric pressure in maintaining the reactor are balanced for the reactor can steady operation in succession, and the methane that can also utilize self storage simultaneously keeps the inside headspace of reactor to have methane gas all the time, in order to do benefit to methane anaerobic oxidation microorganism's enrichment.
The reactor main body 1 is internally used as a main area for enrichment culture of methane anaerobic oxidation microorganisms, the utilization rate of methane is improved through continuous cyclic aeration and hydraulic circulation in the device, mass transfer is enhanced, and favorable conditions are provided for enrichment culture of the methane anaerobic oxidation microorganisms. The concrete structure is as follows: the three-phase separator 8 is hung below the cover plate 5 through a connecting rod 21, the upper part of the three-phase separator 8 is in an inverted funnel shape, the lower part of the three-phase separator 8 is in a vertical cylindrical shape, and the diameter of the lower part of the three-phase separator is equal to that of the outer wall 7 of the lower part of the reactor. The lower part of the three-phase separator 8 is coaxially provided with a guide cylinder 9, the upper part of the guide cylinder 9 is in an inverted funnel shape, a section of the guide cylinder extends into the three-phase separator 8, and the lower part of the guide cylinder is in a vertical cylinder shape. The upper part of the outer wall 6 at the upper part of the reactor is cylindrical, the diameter of the lower part of the outer wall is gradually reduced to form a funnel-shaped structure, the bottom of the outer wall is positioned below the three-phase separator 8, and an annular opening with a certain distance is reserved between the outer wall 6 at the upper part of the reactor and the bottom of the side wall of the three-phase separator 8. The outer wall 7 of the lower part of the reactor is cylindrical, and the side wall of the reactor is provided with a water inlet 14 and a bottom sludge discharge port 15. The bottom of the outer wall 7 at the lower part of the reactor is provided with a bottom conical structure 16, the surface of the bottom conical structure 16 is formed by rotating a circle of a semicircular arc with an upward opening to form an annular concave cavity, and the bottom surface of the outer wall 7 at the lower part of the reactor is sealed by the bottom conical structure 16. And the bottom of the guide cylinder 9 extends into the annular concave cavity, but the bottom of the guide cylinder and the annular concave cavity keep a distance, so that a guide structure capable of guiding water flow to rotate 180 degrees and turn round and flow upwards is formed. The liquid storage tank 17 is a sealed tank body, and a second methane bag connecting port 18 and a water inlet pipe 19 are arranged on a sealing cover plate above the tank body. The second methane bag is a deformable bag filled with a large amount of methane gas, the gas outlet of the second methane bag is communicated with the headspace of the liquid storage tank 17 through a second methane bag connecting port 18, and the second methane bag has the function of keeping the balance of internal and external air pressures by introducing equal amount of methane gas when the culture medium in the tank body is pumped out, and always keeping the anaerobic environment of the internal culture medium. One end of the water inlet pipe 19 extends into the liquid level in the liquid storage tank 17, and the other end is connected with the water inlet 14 through the peristaltic pump 20. In the operation process, the peristaltic pump 20 can inject the fresh culture medium in the liquid storage tank 17 into the liquid storage tank 17 through the water inlet 14, and the fresh culture medium is mixed with the original internal culture medium and then quickly mixed through the position of the aeration head, so that the influence of the change of the substrate concentration in the fresh culture solution on methane anaerobic oxidation microorganisms is avoided.
In the device, the inner cavity of the outer wall 7 at the lower part of the reactor is divided into two parts by the guide shell 9, a reaction area is arranged in the guide shell 9, a circulation area is arranged outside the guide shell 9, and water flow continuously circulates back and forth between the reaction area and the circulation area. The power of circulation comes from the stripping action, specifically: a gas circulating pump 22 is arranged outside the reactor, and two ends of the gas circulating pump are respectively connected with a gas inlet 23 and a gas outlet 24 through pipelines. An aeration head 26 is arranged at the position close to the bottom conical structure 16 at the lower part of the inner part of the guide shell 9, and the lower end of the air outlet 24 is connected with the aeration head 26 at the lower part of the inner part of the guide shell 9 through a rubber pipe 25. Thus, under the action of the gas circulation pump 22, the gas containing a large amount of methane in the reactor headspace can be continuously pumped into the aeration head 26, and then escapes in the form of micro bubbles and floats upwards, so that a stripping action is formed to push the mud-water mixture to flow upwards, and the inverted funnel at the upper part of the guide cylinder 9 can strengthen the stripping action. After the slurry mixture enters the three-phase separator 8, the gas is recycled into the reactor headspace and the slurry mixture enters the recycle zone and flows downward again.
Sludge-water separation is carried out between the outer wall 6 at the upper part of the reactor and the three-phase separator 8 as a settling zone, and the lower part of the settling zone is communicated with the circulating zone through an annular opening, so that after the sludge-water mixture enters the settling zone, particles are precipitated due to small disturbance at the position, and then the particles are returned to the circulating zone again. An overflow port 27 is arranged on one side of the outer wall 6 at the upper part of the reactor, the overflow port 27 is communicated with a water outlet weir 29, a water outlet 28 is arranged on the side wall of the water outlet weir 29, and the height of the water outlet 28 is higher than that of the overflow port 27. The clarified water enters the weir 29 through the overflow 27 and exits through the outlet 28. In this device, the water outlet 28 is a pipe inclined downward.
The horizontal inclination angle of the funnel-shaped structure of the outer wall 6 at the upper part of the reactor is set to be 55-60 degrees, the horizontal inclination angle of the inverted funnel-shaped structure at the upper part of the guide shell 9 is set to be 55-60 degrees, and the inclination angles of the funnel-shaped structure and the inverted funnel-shaped structure are kept consistent. The height-diameter ratio of the outer wall 7 at the lower part of the reactor is set to be 3-5: 1. In the guide shell 9, the height-diameter ratio of the cylindrical part of the reaction zone is set to be 8-10: 1, and the ratio of the inner diameter of the reaction zone to the outer diameter of the circulating zone is set to be 1-1.5: 2. The width of the annular opening is set to be 1-2 cm. The diameter of the semicircular arc that is rotated to form the bottom conical structure 16 is one-half of the diameter of the outer wall 7 at the lower part of the reactor.
In the present apparatus, in order to ensure the enrichment effect of anaerobic methane-oxidizing microorganisms, the reactor body 1 should be sealed from the outside atmosphere to reduce the internal oxygen content as much as possible.
Based on the device, the continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment method can also be provided, and the steps are as follows:
firstly, a culture medium for culturing methane anaerobic oxidation microorganisms is injected into the liquid storage tank 17 and the reactor main body 1, and the headspace of the liquid storage tank 17 and the reactor main body 1 is ensured to be in an anaerobic environment filled with methane gas; and simultaneously, the first methane gas bag and the second methane gas bag are filled with methane gas. The inside of the reactor main body 1 is previously inoculated with microorganisms.
Then, the enrichment culture of methane anaerobic oxidation microorganisms is started, fresh liquid culture medium in a liquid storage tank 17 continuously enters a circulation zone of the reactor through a water inlet pipe 19 and a water inlet 14 under the action of a peristaltic pump 20, and reaches the bottom of the reactor along with downward water flow of the circulation zone, a gas circulation pump 22 is kept started, methane in a top air phase in the reactor flows into the gas circulation pump 22 through a gas inlet 23, escapes from an aeration head 26 in a bubble form through a gas outlet 24 and a rubber pipe 25, forms a gas stripping action in the reaction zone, liquid in the circulation zone enters the reaction zone under the flow guide action of a conical structure 16 at the bottom, then continuously rises to enter a three-phase separator 8 for three-phase separation, a mud-water mixture enters the circulation zone again to form liquid-phase circulation, the liquid level of the bubbles in the three-phase separator 8 is extinguished, and the gas enters the top space of the reactor again, forming gas circulation; part of the mud-water mixture passes through an annular opening between the lower end of the three-phase separator 8 and the outer wall 6 at the upper part of the reactor, the upward flow enters a settling zone to realize mud-water separation, the settled sludge slides into a circulating zone through the annular opening again, and the clarified water enters an effluent weir 29 through an overflow port 27 and is discharged through a water outlet 28; wherein the ratio of the air inlet flow controlled by the gas circulating pump 22 to the water inlet flow controlled by the peristaltic pump 20 is 0.1-0.5: 1.
In the enrichment culture process, the first methane gas bag is utilized to keep the gas pressure of the gas phase space of the reactor balanced with the ambient atmospheric pressure, and the internal headspace of the reactor is kept to be always provided with methane gas; the second methane bag is utilized to keep the air pressure in the liquid storage tank 17 balanced with the ambient atmospheric pressure and keep the anaerobic environment of the liquid culture medium in the liquid storage tank 17; meanwhile, the dissolved oxygen concentration in the liquid phase is continuously monitored by the dissolved oxygen probe 12, the pH in the liquid phase is monitored by the pH probe 13, and a gas sample is periodically taken by the gas taking port 10 for gas phase component analysis, so that the normal operation of the reactor is ensured.
When the biomass in the reactor main body 1 reaches 1.5-2 g VSS/L, closing the peristaltic pump 20 and the gas circulating pump 22, standing to deposit precipitates at the bottom of the reactor, and then taking out 40-50% of the enriched culture through a bottom sludge discharge port 15; after the culture medium is taken out, the peristaltic pump 20 and the gas circulating pump 22 are started again, and a new round of enrichment culture is started again.
The above-mentioned embodiment is just a preferred scheme of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit and scope of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications should fall within the protection scope of the claims of the present invention.
Claims (9)
1. A continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device is characterized by comprising a reactor main body (1), a water inlet system (2), an aeration system (3) and a water outlet system (4); the reactor main body (1) comprises a reactor cover plate (5), an upper outer wall (6) of the reactor, a lower outer wall (7) of the reactor, a three-phase separator (8), a guide cylinder (9), an air intake (10), a first methane gas bag connecting port (11), a dissolved oxygen probe (12), a pH probe (13), a water inlet (14), a bottom sludge discharge port (15) and a bottom conical structure (16); the water inlet system (2) comprises a liquid storage tank (17), a second methane bag connecting port (18), a water inlet pipe (19) and a peristaltic pump (20); the aeration system (3) comprises a gas circulating pump (22), a gas inlet (23), a gas outlet (24), a rubber pipe (25) and an aeration head (26); the water outlet system (4) comprises an overflow port (27), a water outlet (28) and a water outlet weir (29);
the reactor main body (1) is formed by connecting an upper outer wall (6) of the reactor with a lower outer wall (7) of the reactor; a cover plate (5) is arranged at the upper part of the reactor main body (1), and an air intake (10), a first methane gas bag connecting port (11), a dissolved oxygen probe (12) and a pH probe (13) are respectively arranged on the cover plate (5); one end of the gas taking port (10) is sealed by a rubber plug, and the other end of the gas taking port penetrates through the cover plate (5) and is communicated with the reactor main body (1); the first methane gas bag is communicated with the headspace of the inner cavity of the reactor main body (1) through a first methane gas bag connecting port (11); a three-phase separator (8) is hung below the cover plate (5) through a connecting rod (21), the upper part of the three-phase separator (8) is in an inverted funnel shape, the lower part of the three-phase separator (8) is in a vertical cylindrical shape, and the diameter of the lower part of the three-phase separator is equal to that of the outer wall (7) of the reactor; a guide cylinder (9) is coaxially arranged below the three-phase separator (8), the upper part of the guide cylinder (9) is in an inverted funnel shape and extends into the three-phase separator (8), and the lower part of the guide cylinder is in a vertical cylinder shape; the upper part of the outer wall (6) at the upper part of the reactor is cylindrical, the diameter of the lower part of the outer wall is gradually reduced to form a funnel-shaped structure, the bottom of the outer wall is positioned below the three-phase separator (8), and an annular opening with a certain distance is reserved between the outer wall (6) at the upper part of the reactor and the bottom of the side wall of the three-phase separator (8); the outer wall (7) at the lower part of the reactor is cylindrical, and the side wall of the reactor is provided with a water inlet (14) and a bottom sludge discharge port (15); the bottom of the outer wall (7) at the lower part of the reactor is provided with a bottom conical structure (16), and the surface of the bottom conical structure (16) is formed by rotating a circle of a semicircular arc with an upward opening to form an annular concave cavity; the bottom of the guide cylinder (9) extends into the annular concave cavity, but the bottom of the guide cylinder and the annular concave cavity keep a distance; the liquid storage tank (17) is a sealed tank body, and a second methane bag connecting port (18) and a water inlet pipe (19) are arranged above the tank body; the second methane bag is communicated with the headspace of the liquid storage tank (17) through a second methane bag connecting port (18); one end of the water inlet pipe (19) extends into the liquid level of the liquid storage tank (17), and the other end is connected with the water inlet (14) through a peristaltic pump (20); the gas circulating pump (22) is positioned outside the reactor, the two ends of the gas circulating pump are respectively connected with the gas inlet (23) and the gas outlet (24), and the lower end of the gas outlet (24) is connected with an aeration head (26) positioned below the inner part of the guide shell (9) through a rubber pipe (25); an overflow port (27) is arranged on one side of the outer wall (6) at the upper part of the reactor, the overflow port (27) is communicated with a water outlet weir (29), a water outlet (28) is arranged on the side wall of the water outlet weir (29), and the height of the water outlet (28) is higher than that of the overflow port (27).
2. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the horizontal inclination angle of the funnel-shaped structure of the outer wall (6) at the upper part of the reactor is 55-60 degrees, the horizontal inclination angle of the inverted funnel-shaped structure at the upper part of the guide shell (9) is 55-60 degrees, and the inclination angles are kept consistent.
3. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the height-diameter ratio of the outer wall (7) at the lower part of the reactor is 3-5: 1.
4. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the inner cavity of the outer wall (7) at the lower part of the reactor is divided into two parts by the guide cylinder (9), a reaction area is arranged in the guide cylinder (9), a circulation area is arranged outside the guide cylinder (9), the height-diameter ratio of the reaction area is 8-10: 1, and the diameter ratio of the reaction area to the circulation area is 1-1.5: 2.
5. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the width of the annular opening is 1-2 cm.
6. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the diameter of the semicircular arc which rotates to form the bottom conical structure (16) is half of the diameter of the outer wall (7) at the lower part of the reactor.
7. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the water outlet (28) is a pipeline inclined downwards.
8. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the first methane gas bag and the second methane gas bag are filled with methane gas.
9. The continuous flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device of claim 1, characterized in that: the reactor main body (1) is closed and isolated from the outside atmosphere.
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| CN110184176A (en) * | 2019-04-25 | 2019-08-30 | 浙江大学 | Continuous flow gas-liquid cycle methane anaerobic oxidized Enrichment by Microorganisms acquisition means and its method |
| CN110184176B (en) * | 2019-04-25 | 2024-06-11 | 浙江大学 | Continuous-flow gas-liquid circulation methane anaerobic oxidation microorganism enrichment device and method thereof |
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