CN210155409U

CN210155409U - Miniature microscopic imaging lens

Info

Publication number: CN210155409U
Application number: CN201921267754.8U
Authority: CN
Inventors: 不公告发明人
Original assignee: Suzhou Yibolun Photoelectric Instrument Co Ltd
Current assignee: Suzhou Yibolun Photoelectric Instrument Co Ltd
Priority date: 2019-01-24
Filing date: 2019-08-06
Publication date: 2020-03-17
Anticipated expiration: 2029-08-06
Also published as: CN110794564A; CN210155408U; CN111474697A; CN211086791U

Abstract

The utility model relates to an optical imaging technical field discloses a miniature microscopic imaging camera lens, including objective and focusing lens, objective includes a plurality of objective lenses, focusing lens includes a plurality of focusing lens lenses, objective with the focusing lens separation, objective with be provided with dichroic mirror scanner between the focusing lens, dichroic mirror scanner is located the back focal plane of the objective that calculates according to the exciting light wavelength. The utility model discloses an objective and focusing lens separation, the design of middle dichroic mirror scanner that inserts makes miniature microscopic imaging lens's volume reduce greatly, can satisfy the rigidity part simultaneously and be less than 3 millimeters, can satisfy the lens diameter again and be less than 2 millimeters, and numerical aperture is greater than 0.7's requirement.

Description

Miniature microscopic imaging lens

Technical Field

The utility model relates to an optical imaging technical field, concretely relates to miniature microscopic imaging camera lens.

Background

Micro-objectives play a very important role in endoscopes and other micro-imaging devices. In particular for fluorescence microscopy, single photon excitation fluorescence Imaging with a medium-low Numerical Aperture (NA <0.6) micro-objective has been used in gastrointestinal AutoFluorescence Imaging (AFI) and Confocal laser (Confocal) endoscopic Imaging. For such micro-objectives, several technical solutions can be generally adopted: 1. a gradient index lens based solution that is suitable for applications where the diameter is very small (lens diameter down to 0.3 mm), but the rigid part length is not critical; 2. the scheme based on the multi-piece spherical lens is suitable for the large-diameter (more than 2 mm of the lens diameter) and low in cost, and glass or polymer can be used as a material; 3. the proposal comprising a plurality of aspheric lenses is suitable for the application with larger diameter (the diameter of the lens is more than 2.2-2.3 mm, the domestic general processing standard) but has higher requirement on the length of the rigid part. For single-photon excitation fluorescence imaging, since dyes are abundant in types and excitation wavelengths are many, chromatic aberration elimination in the visible wavelength range is important.

In recent years, nonlinear optical imaging represented by two-photon excitation fluorescence imaging has gradually entered the field of endoscopic imaging. Second-order nonlinear optical effects such as Two-photon excitation fluorescence (TPEF), Two-photon excitation autofluorescence (TPEAF), and Second harmonic generation (Second harmonic generation, SHG) require a numerical aperture of the objective lens of > 0.7-0.8; three-order nonlinear optical effects such as Three-photon excitation fluorescence (TriPEF), Third Harmonic Generation (THG), and Coherent anti-stokes Raman Scattering (CARS) require an objective lens with a numerical aperture > 1.0. Such a high required numerical aperture presents a great technical challenge for the design and manufacture of miniature microobjectives. At present, the micro microscope objective aiming at the second-order nonlinear optical effect in the world has the following implementation schemes: 1. the proposal of the gradient refractive index lens and the high refractive index hemispherical lens is represented by German GRINTECH company, the high refractive index hemispherical lens is used for increasing the numerical aperture to 0.8, and the diameter is small; 2. the scheme based on the multi-piece spherical lens is suitable for the large-diameter (more than 2 mm of the lens diameter) and the material can be glass or polymer, so that the cost is low, but the numerical aperture is not more than 0.8. For nonlinear optical imaging, achromatic is less important because of the single application, fixed dye species, and fixed excitation wavelength. For non-sectioning biological sample imaging, the working distance of the objective lens is important, while the field curvature is less important.

At present, no micro microscope objective product or research result exists in the world, and the micro microscope objective product or research result can simultaneously meet the requirements that the rigid part is less than 3 mm, the diameter of the lens is less than 2 mm, and the numerical aperture is more than 0.7.

SUMMERY OF THE UTILITY MODEL

The utility model discloses can't satisfy the rigidity part simultaneously and be less than 3 millimeters to current a miniature micro objective for nonlinear optics formation of image, can satisfy again that the lens diameter is less than 2 millimeters, numerical aperture is greater than 0.7's requirement, has provided a miniature micro imaging camera lens, can satisfy the rigidity part simultaneously and be less than 3 millimeters, can satisfy again that the lens diameter is less than 2 millimeters, and numerical aperture is greater than 0.7's requirement.

In order to achieve the above object, the utility model adopts the following technical scheme:

the utility model provides a miniature microscopic imaging lens, includes objective and focusing lens, objective includes a plurality of objective lens, focusing lens includes a plurality of focusing lens lenses, objective with the focusing lens separation, objective with be provided with the dichroic mirror scanner between the focusing lens, the dichroic mirror scanner is located the back focal plane according to the objective that the exciting light wavelength calculated.

Compared with the prior art, the utility model discloses a principle and beneficial effect: when the device is used, laser (excitation laser) input by a laser input optical fiber is reflected to a preset position (objective lens) through a dichroic mirror scanner, a nonlinear optical signal excited by an observed object passes through the objective lens and then reaches the dichroic mirror scanner, the dichroic mirror scanner enables the nonlinear optical signal to transmit and pass through, the nonlinear optical signal is received by a focusing lens, and then the focusing lens focuses the received nonlinear optical signal on the surface of the laser output optical fiber, so that the observation of the observed object is completed.

The utility model discloses an objective and focusing lens separation, the design of middle dichroic mirror scanner that inserts makes miniature microscopic imaging lens's volume reduce greatly, can satisfy the rigidity part simultaneously and be less than 3 millimeters, can satisfy the lens diameter again and be less than 2 millimeters, and numerical aperture is greater than 0.7's requirement.

Further, the dichroic mirror scanner is capable of mechanical rotation. The effect of changing the reflection angle of the laser can be achieved by rotating the dichroic mirror scanner.

Further, the dichroic mirror scanner comprises a dichroic mirror and an annular driver not affecting the transmission of the nonlinear optical signal, the dichroic mirror being overlaid on the annular driver. In this scheme, replaced the speculum with the dichroic lens, and the transmission of nonlinear optical signal can not influenced by the driver in this scheme, and the dichroic lens has played the effect of dichroic mirror among the prior art promptly, has also reached the effect that lets the driver change the laser reflection angle, but also can reach reduction element quantity, makes whole dichroic mirror scanner volume littleer, weight is lighter.

Furthermore, the dichroic lens is made of fused quartz. The surface of the quartz is easy to clean by adopting a fused quartz material.

Further, the objective lens is manufactured by adopting a die pressing processing technology. So that the manufacture is more convenient.

Furthermore, the objective lens is made of optical glass or high molecular polymer. The objective lens under the material has the advantage of light weight.

Further, the focusing lens is manufactured by adopting a mould pressing processing technology. So that the manufacture is more convenient.

Furthermore, the focusing lens is made of optical glass or high molecular polymer. The focusing lens has the advantages of light weight.

Further, the numerical aperture of the objective lens is any value between 0.5 and 1.2. The resolution of the objective lens is better in the range, so that the imaging effect is better.

Further, the working distance of the objective lens in the air is any value between 0.1 mm and 2 mm. The visual field observed in the range is better, and the observation of the object is more facilitated.

Drawings

Fig. 1 is a schematic view of an optical structure according to an embodiment of the present invention;

fig. 2 is a schematic perspective view of a dichroic mirror scanner according to an embodiment of the present invention;

fig. 3 is an excitation light focal plane vignetting diagram of the objective lens according to an embodiment of the present invention;

fig. 4 is a focal vignetting diagram of the emitted light of the focusing lens in the embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: a first objective lens piece 1, a second objective lens piece 2, a first focusing lens piece 3, a second focusing lens piece 4, a dichroic mirror scanner 5, a dichroic piece 6, a driver 7.

The embodiment is basically as shown in the attached figures 1 to 4:

a micro microscopic imaging lens comprises an objective lens and a focusing lens, wherein the objective lens and the focusing lens are arranged separately, and both the objective lens and the focusing lens are manufactured by adopting a mould pressing processing technology; in this embodiment, the objective lens and the focusing lens are made of optical glass or high molecular polymer. The numerical aperture of the objective lens is any numerical value between 0.5 and 1.2, and the numerical aperture of the focusing objective lens is any numerical value between 0.3 and 1.1; in this embodiment, the numerical aperture of the objective lens is 0.815 and the length is 1 mm; the focusing objective has a numerical aperture of 0.4 and a length of 2.227 millimeters.

The objective lens comprises a first objective lens 1 and a second objective lens 2, the first objective lens 1 having an opposite object-side surface S11 and an opposite image-side surface S12, the second objective lens 2 having an opposite object-side surface S21 and an opposite image-side surface S22; the focusing lens includes a first focusing lens piece 3 and a second focusing lens piece 4, the first focusing lens piece 3 having an opposite object side surface S31 and an opposite image side surface S32, the second focusing lens piece 4 having an opposite object side surface S41 and an opposite image side surface S42.

Among them, the objective lens needs to satisfy the conditions as listed in table 1.

TABLE 1

The focusing lens objective needs to satisfy the conditions as listed in table 1.

TABLE 2

A dichroic mirror scanner 5 is arranged between the objective lens and the focusing lens, the dichroic mirror scanner 5 being located in the back focal plane of the objective lens calculated from the wavelength of the excitation light. The dichroic mirror scanner 5 includes a dichroic mirror 6 and an annular driver 7 that does not affect transmission of the nonlinear optical signal, the dichroic mirror 6 covers the annular driver 7, in this embodiment, the dichroic mirror 6 is circular, the diameter of the dichroic mirror is 0.8 mm, the thickness of the dichroic mirror is 0.145 mm, and the material of the dichroic mirror is fused quartz. The driver 7 is a micro-electromechanical driver 7.

When specifically implementing, will the utility model discloses a miniature microscopic imaging lens installs in miniature probe, through laser input fiber emission laser, rethread dichroic mirror scanner 5 reflects laser (excitation laser) to preset position (objective) with the laser input fiber input, reachs the surface of dichroic mirror scanner 5 behind objective by the nonlinear optical signal of observing the object excitation, dichroic mirror scanner 5 lets nonlinear optical signal transmission, pass, nonlinear optical signal is received by focusing lens, focusing lens focuses on laser output fiber surface with the nonlinear optical signal received afterwards, thereby let miniature probe accomplish the observation to being observed the object. In the process, the dichroic mirror 6 is driven by the driver 7 to rotate in two dimensions, so that the dichroic mirror scanner 5 scans the two-dimensional point of the object.

As shown in fig. 3, the excitation light focal plane vignetting pattern of the objective lens achieves a transmission efficiency of greater than 0.9 for the excitation light wavelength at all viewing angles.

As shown in fig. 4, the focal plane vignetting of the emitted light from the focusing lens achieves a transmission efficiency of greater than 0.9 at wavelengths of emitted light with a field of view of less than 0.077 mm.

The above description is only an example of the present invention, and the common general knowledge of the known specific structures and characteristics of the embodiments is not described herein. It should be noted that variations and modifications can be made by those skilled in the art without departing from the structure of the invention. These should also be considered as the scope of protection of the present invention, and these do not affect the effect of the implementation of the present invention and the utility of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. A miniature microscopic imaging lens comprises an objective lens and a focusing lens, and is characterized in that: the objective lens comprises a plurality of objective lens lenses, the focusing lens comprises a plurality of focusing lens lenses, the objective lens is separated from the focusing lens, a dichroic mirror scanner is arranged between the objective lens and the focusing lens, and the dichroic mirror scanner is located on a back focal plane of the objective lens calculated according to the wavelength of the excitation light.

2. The micro-microscopic imaging lens according to claim 1, characterized in that: the dichroic mirror scanner is capable of mechanical rotation.

3. The micro-microscopic imaging lens according to claim 2, characterized in that: the dichroic mirror scanner comprises a dichroic mirror and an annular driver which does not affect the transmission of the nonlinear optical signal, and the dichroic mirror is covered on the annular driver.

4. The micro-microscopic imaging lens according to claim 3, characterized in that: the dichroic lens is made of fused quartz.

5. The micro-microscopic imaging lens according to claim 1, characterized in that: the objective lens is manufactured by adopting a mould pressing processing technology.

6. The micro-microscopic imaging lens according to claim 1, characterized in that: the objective lens is made of optical glass or high molecular polymer.

7. The micro-microscopic imaging lens according to claim 1, characterized in that: the focusing lens is manufactured by adopting a mould pressing processing technology.

8. The micro-microscopic imaging lens according to claim 1, characterized in that: the focusing lens is made of optical glass or high molecular polymer.

9. The micro-microscopic imaging lens according to claim 1, characterized in that: the numerical aperture of the objective lens is any value between 0.5 and 1.2.

10. The micro-microscopic imaging lens according to claim 1, characterized in that: the working distance of the objective lens in the air is any value between 0.1 mm and 2 mm.