CN210140582U

CN210140582U - Water bath for cell resuscitation

Info

Publication number: CN210140582U
Application number: CN201920828537.5U
Authority: CN
Inventors: 钟宏辉
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-05-31
Filing date: 2019-05-31
Publication date: 2020-03-13
Anticipated expiration: 2029-05-31

Abstract

The utility model discloses a water bath for cell resuscitation, including the water bath shell, the both sides inner wall of water bath shell is provided with the fixing base, and one side outer wall of fixing base has the heating pipe through bolted connection, one side outer wall welding of water bath shell has the backup pad, and the top outer wall of backup pad has the water pump through bolted connection, one side outer wall welding of water pump has the inlet tube, and one side outer wall welding that the inlet tube was kept away from to the water pump has the outlet pipe, the top outer wall of water bath shell is provided with the recess, and the inner wall sliding connection of recess has the pot cover, the top outer wall of pot cover has the block through bolted connection. The utility model discloses can revive big batch cell, effectively improve the quantity and the work efficiency that the cell was revived, can constantly stir the water in the water bath, realize that the temperature is balanced, ensure that the cell in the cryopreserving pipe melts rate unanimous basically, effectively improve the survival rate of cell to the contaminated probability of cell has been reduced.

Description

Water bath for cell resuscitation

Technical Field

The utility model relates to a cell recovery technical field especially relates to a water-bath for cell recovery.

Background

The thawing of frozen cells is a technical means commonly used in biology and medicine, the principle of the thawing is to rapidly thaw the frozen cells in water bath at 37 ℃ so as to reduce the damage of DMSO (dimethyl sulfoxide) to the cells during cell thawing, and the traditional cell thawing method is to directly put a frozen tube into the water bath at 37 ℃ and shake the frozen tube by hand continuously so as to rapidly thaw the frozen tube.

At present, the method for resuscitating cells manually has certain disadvantages, the tube mouth of the cryopreservation tube easily invades into the water bath during the manual shaking process, so that the probability of cell pollution during the resuscitating process is increased, the number of the cells resuscitated by the manual resuscitating method is limited, large-batch cell resuscitating operation cannot be performed, and the number and the working efficiency of cell resuscitating are very limited, so that the water bath kettle for cell resuscitating needs to be designed to solve the problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing a water bath kettle for cell resuscitation.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a water bath for cell resuscitation comprises a water bath shell, wherein fixing seats are arranged on the inner walls of two sides of the water bath shell, a heating pipe is connected to the outer wall of one side of each fixing seat through a bolt, a supporting plate is welded to the outer wall of one side of the water bath shell, a water pump is connected to the outer wall of the top of the supporting plate through a bolt, a water inlet pipe is welded to the outer wall of one side of the water pump, a water outlet pipe is welded to the outer wall of one side, away from the water inlet pipe, of the water pump, a groove is formed in the outer wall of the top of the water bath shell, a pot cover is connected to the inner wall of the groove in a sliding mode, a cover cap is connected to the outer wall of the top of the pot cover through a bolt, a motor shell is connected to the inner wall of the top of, the outer wall of the bottom of the second movable rod is connected with a second connecting rod through a pin shaft.

As a further aspect of the present invention: the outer wall of one side of second connecting rod is provided with places the dish, and places the top outer wall of dish and open and have the equidistance and be the spacing hole that the annular distributes.

As a further aspect of the present invention: the inner wall of the limiting hole is bonded with a sealing ring, and the inner wall of the sealing ring is connected with a freezing tube in a sliding mode.

As a further aspect of the present invention: the outer wall threaded connection who places the dish has the screw cap, and the welding of the top outer wall of screw cap has the bracing piece, and the welding of the top outer wall of bracing piece has the carousel.

As a further aspect of the present invention: the bottom outer wall of water bath shell is provided with the stand, and the quantity of stand is two, and the outer wall welding of stand has the bearing, and the outer wall welding of bearing has first movable rod.

As a further aspect of the present invention: the bottom outer wall of the first movable rod is connected with a first connecting rod through a pin shaft, and the outer wall of one side of the first connecting rod is welded with the outer wall of one side of the placing disc.

As a further aspect of the present invention: the heating pipe, the water pump and the motor are all connected with a switch through leads, and the switch is connected with a power supply through leads.

The utility model has the advantages that:

1. the motor is driven to start working through the placing plate, the motor, the second movable rod and the second connecting rod, the second connecting rod can be driven to rotate through the second movable rod, and then the placing plate is driven to rotate, so that cell sap in the cryopreservation tube on the placing plate generates a certain centrifugal force to replace manual shaking, cells in a large batch can be revived, and the cell reviving quantity and the working efficiency are effectively improved;

2. through the arranged water pump, the water inlet pipe and the water outlet pipe, the water pump can be driven to start working in the process of cell resuscitation, water in the water bath pot is pumped into the water pump through the water inlet pipe and then is discharged through the water outlet pipe, the water in the water bath pot can be continuously stirred, the balance of water temperature is realized, the cell thawing rate in the cryopreservation pipe is ensured to be basically consistent, and the survival rate of cells is effectively improved;

3. through the screw thread lid and the sealing washer that set up, place the screw thread lid that sets up on the dish, prevent effectively that water bath liquid from getting into to freeze in depositing the pipe, avoid the contaminated probability of cell, and place and coil the sealing washer not only can increase its and freeze the frictional force between the pipe, make fixed more stable, can also prevent that water bath liquid from overflowing through spacing hole and placing on the dish, further reduced the contaminated probability of cell.

Drawings

Fig. 1 is a schematic front view of a water bath for cell resuscitation according to the present invention;

fig. 2 is a schematic bottom sectional view of a water bath for cell resuscitation according to the present invention;

fig. 3 is a schematic structural diagram of a position a of the water bath for cell resuscitation according to the present invention.

In the figure: the automatic control device comprises a water bath kettle shell 1, a heating pipe 2, a first connecting rod 3, a supporting plate 4, a water pump 5, a water inlet pipe 6, a first movable rod 7, a vertical column 8, a pot cover 9, a cover cap 10, a motor shell 11, a motor 12, a rotating shaft 13, a second movable rod 14, a water outlet pipe 15, a sealing ring 16, a freezing storage pipe 17, a placing plate 18, a threaded cover 19, a supporting rod 20, a rotating disc 21 and a second connecting rod 22.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a water bath for cell resuscitation comprises a water bath shell 1, wherein fixing seats are welded on the inner walls of two sides of the water bath shell 1, a heating pipe 2 is connected on the outer wall of one side of each fixing seat through a bolt, a supporting plate 4 is welded on the outer wall of one side of the water bath shell 1, a water pump 5 is connected on the outer wall of the top of the supporting plate 4 through a bolt, a water inlet pipe 6 is welded on the outer wall of one side of the water pump 5, a water outlet pipe 15 is welded on the outer wall of one side of the water pump 5 away from the water inlet pipe 6, a groove is formed in the outer wall of the top of the water bath shell 1, a pot cover 9 is connected on the inner wall of the top of the groove in a sliding manner, a cover cap 10 is connected on the outer wall of the top of the pot cover 9 through a bolt, and the outer wall of the rotating shaft 13 is welded with a second movable rod 14, and the outer wall of the bottom of the second movable rod 14 is connected with a second connecting rod 22 through a pin shaft.

In the utility model, a placing plate 18 is welded on the outer wall of one side of a second connecting rod 22, the outer wall of the top part of the placing plate 18 is provided with limit holes which are distributed in an annular shape at equal intervals, the inner wall of each limit hole is bonded with a sealing ring 16, the inner wall of the sealing ring 16 is connected with a freezing tube 17 in a sliding way, the outer wall of the placing plate 18 is connected with a screw cap 19 in a threaded way, the outer wall of the top part of the screw cap 19 is welded with a support rod 20, the outer wall of the top part of the support rod 20 is welded with a rotary plate 21, the outer wall of the bottom part of a water bath kettle shell 1 is welded with two upright columns 8, the outer wall of each upright column 8 is welded with a bearing, the outer wall of the bearing is welded with a first movable rod 7, the outer wall of the bottom part of the first movable rod 7 is connected with a first connecting rod 3 through, and the switch is connected with a power supply through a lead.

The working principle is as follows: when the device is used, the heating pipe 2 is electrified to heat the water bath liquid, the freezing pipe 17 is placed in the limiting hole on the placing disc 18, the driving motor 12 starts to work, the second connecting rod 22 can be driven to rotate through the second movable rod 14, the placing disc 18 is further driven to rotate, the cell liquid in the freezing pipe 17 on the placing disc 18 generates a certain centrifugal force to replace manual shaking, large batches of cells can be revived, the water pump 5 can be driven to start to work in the process of cell reviving, water in the water bath kettle is pumped into the water pump 5 through the water inlet pipe 6 and then is discharged through the water outlet pipe 15, the water in the water bath kettle can be continuously stirred, the balance of the water temperature is realized, the cell melting speed in the freezing pipe 17 is basically consistent, the threaded cover 19 arranged on the placing disc 18 effectively prevents the water bath liquid from entering the freezing pipe 17, the sealing ring 16 on the placing disc 18 can increase the friction force between the freezing pipe 17 and the water bath liquid, the fixation is more stable.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A water bath for cell resuscitation comprises a water bath shell (1) and is characterized in that fixing seats are arranged on inner walls of two sides of the water bath shell (1), a heating pipe (2) is connected to an outer wall of one side of each fixing seat through a bolt, a supporting plate (4) is welded to an outer wall of one side of the water bath shell (1), a water pump (5) is connected to an outer wall of the top of each supporting plate (4) through a bolt, a water inlet pipe (6) is welded to an outer wall of one side of each water pump (5), a water outlet pipe (15) is welded to an outer wall of one side, far away from the water inlet pipe (6), of each water pump (5), a groove is formed in the outer wall of the top of the water bath shell (1), a pot cover (9) is slidably connected to the inner wall of the groove, a cover cap (10) is connected to the outer wall of the top of the pot cover (, and the top inner wall of motor housing (11) has motor (12) through bolted connection, the output shaft one end welding of motor (12) has axis of rotation (13), and the outer wall welding of axis of rotation (13) has second movable rod (14), the bottom outer wall of second movable rod (14) has second connecting rod (22) through round pin hub connection.

2. The water bath kettle for cell resuscitation as claimed in claim 1, wherein a placing plate (18) is arranged on the outer wall of one side of the second connecting rod (22), and limiting holes are formed in the outer wall of the top of the placing plate (18) and are distributed annularly at equal intervals.

3. The water bath kettle for cell resuscitation as claimed in claim 2, wherein a sealing ring (16) is bonded to the inner wall of the limiting hole, and a freezing tube (17) is slidably connected to the inner wall of the sealing ring (16).

4. The water bath kettle for cell resuscitation as claimed in claim 3, wherein the outer wall of the placing plate (18) is in threaded connection with a threaded cover (19), a supporting rod (20) is welded on the outer wall of the top of the threaded cover (19), and a rotating plate (21) is welded on the outer wall of the top of the supporting rod (20).

5. The water bath for cell resuscitation as claimed in claim 4, wherein the outer wall of the bottom of the water bath housing (1) is provided with two upright posts (8), the outer wall of each upright post (8) is welded with a bearing, and the outer wall of each bearing is welded with the first movable rod (7).

6. The water bath kettle for cell resuscitation as claimed in claim 5, wherein the bottom outer wall of the first movable rod (7) is connected with the first connecting rod (3) through a pin shaft, and one side outer wall of the first connecting rod (3) is welded with one side outer wall of the placing disc (18).

7. The water bath for cell resuscitation as claimed in claim 6, wherein the heating tube (2), the water pump (5) and the motor (12) are all connected with a switch through wires, and the switch is connected with a power supply through wires.