CN210127228U - Human umbilical cord mesenchymal stem cell separation device - Google Patents
Human umbilical cord mesenchymal stem cell separation device Download PDFInfo
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- CN210127228U CN210127228U CN201920563548.5U CN201920563548U CN210127228U CN 210127228 U CN210127228 U CN 210127228U CN 201920563548 U CN201920563548 U CN 201920563548U CN 210127228 U CN210127228 U CN 210127228U
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- umbilical cord
- area
- handle
- tool bit
- cleaning
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- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 57
- 238000000926 separation method Methods 0.000 title claims abstract description 45
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 19
- 238000004140 cleaning Methods 0.000 claims abstract description 37
- 238000010008 shearing Methods 0.000 claims abstract description 21
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 17
- 239000010935 stainless steel Substances 0.000 claims abstract description 17
- 238000003860 storage Methods 0.000 claims abstract description 14
- 230000002439 hemostatic effect Effects 0.000 claims abstract description 7
- 239000002184 metal Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000005520 cutting process Methods 0.000 claims description 24
- 210000000080 chela (arthropods) Anatomy 0.000 claims description 11
- 238000005192 partition Methods 0.000 claims description 4
- 230000006978 adaptation Effects 0.000 claims description 3
- 210000003606 umbilical vein Anatomy 0.000 claims description 2
- 238000005406 washing Methods 0.000 abstract description 7
- 206010024796 Logorrhoea Diseases 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 4
- 210000001519 tissue Anatomy 0.000 description 16
- 238000012546 transfer Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 210000000130 stem cell Anatomy 0.000 description 4
- 238000007664 blowing Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000004700 fetal blood Anatomy 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000003892 spreading Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a human umbilical cord mesenchymal stem cell separation device, which comprises a separation box body, wherein the right side of the separation box body is a cleaning area, the left side of the separation box body is provided with a separation area, a shearing area and an appliance storage area, three stainless steel clapboards are fixedly arranged in the cleaning area at intervals, the middle part of each stainless steel clapboard is provided with a movable valve plate, and a water outlet with a valve is arranged on the outer side wall of the cleaning box; four corners in the shearing area are provided with metal hooks, and the left side of the shearing area is also provided with a rectangular opening; the instruments placed in the instrument storage area comprise scissors, hemostatic forceps, umbilical cord forceps and a crochet hook. The utility model discloses a stainless steel material is made, and not only simple structure, washing are convenient, can also guarantee to cut garrulous tissue piece size suitable, labour saving and time saving, operate comfortablely.
Description
Technical Field
The utility model belongs to the technical field of the stem cell separation, a stem cell separator technique is related to, especially a human source umbilical cord mesenchymal stem cell separator.
Background
Umbilical cord Mesenchymal Stem Cells (MSCs) refer to a multifunctional stem cell existing in umbilical cord tissues of newborns, can be differentiated into a plurality of tissue cells, and have wide clinical application prospects in tissue engineering aspects such as bones, cartilages, muscles, tendons, ligaments, nerves, livers, endothelium and cardiac muscles.
During culturing of umbilical cord mesenchymal stem cells, umbilical cord of fetus born in full term by cesarean section is generally taken, washed clean by normal saline in a beaker, then blood vessels are removed by a pair of scissors hemostatic forceps, Fahrenheit jelly tissues inside the blood vessels are stripped, and the obtained tissues are sufficiently sheared into 2-3mm by scissors3Spreading in culture flask, adding culture solution containing 10% FBS, and standing at 37 deg.C under 5% CO2Culturing in an incubator.
The prior separating devices comprise a plurality of beakers, scissors and hemostatic forceps, and are dispersed and inconvenient to use; when the beaker is used for cleaning the umbilical cord, the operation is not easy when the hemostatic forceps are used for cleaning the umbilical cord in the safety cabinet due to the higher inner height of the beaker, the beaker is easy to fall down, and an operation table is polluted; sufficiently cutting the Fahrenheit gum to 2-3mm with common scissors3The method is the most time-consuming and labor-consuming in the whole operation, the tissue size is not easy to control, the problem of large and small tissue blocks can occur when a culture bottle is paved, the phenomenon of floating can occur when the culture bottle is too large or too small in the later period, and the climbing and proliferation of cells are not facilitated.
The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:
1. an umbilical cord tissue mesenchymal stem cell separation device (CN208055308U) comprises a controller, a main body provided with a culture cavity and a separation cavity, and a control door arranged between the culture cavity and the separation cavity, wherein a centrifuge tube rack, a culture dish rack, a first culture bottle placing rack, a centrifuge, a shaking table, a first manipulator, a liquid feeder, a second manipulator, a liquid taking device, a third manipulator, a blowing and beating mechanism and a fourth manipulator are arranged in the separation cavity; the liquid taking device is used for taking out the liquid in the container or adding the taken liquid back to the container; the blowing mechanism is used for blowing the liquid in the container; the centrifugal machine is used for centrifuging the centrifugal tube; the shaking table is used for shaking culture at constant temperature; the first manipulator is used for grabbing the container to move; the second manipulator is used for grabbing the umbilical cord and the particles obtained after the umbilical cord is chopped; the third manipulator is used for cutting the umbilical cord; and the fourth manipulator is used for extruding the umbilical cord blood remained on the umbilical cord.
2. A cord blood stem cell separation device (CN206814738U) comprises a cover body, a separation cavity, a support plate, a storage box, a filter, a transfer pipeline and a transfer bag, wherein the cover body is arranged at the opening at the top of the separation cavity, the back of the separation cavity is fixedly connected with the upper part of the support plate, and the lower part of the support plate is arranged on the storage box; the cover body is provided with a main through hole, an auxiliary through hole and a sealing plug, a sealing plate is arranged in the cover body, and the sealing plug is matched with the main through hole and the auxiliary through hole; the separation cavity comprises an upper accommodating cavity and a neck part, a filter is arranged in the upper accommodating cavity, and the lower part of the upper accommodating cavity is communicated with the neck part; the filter comprises folding filter membranes, an isolation net, a blood guide plate, a flow guide port and a human-shaped membrane, wherein the isolation net is arranged between the folding filter membranes, the blood guide plate is arranged below the folding filter membranes, the flow guide port is arranged in the middle of the blood guide plate, and the human-shaped membrane is arranged in the flow guide port; the neck is provided with a perspective part, the lower end of the neck is provided with a blood outlet, the blood outlet is connected with a transfer pipeline, the transfer pipeline is provided with a flow regulator, the transfer pipeline is divided into a first branch, a second branch and a third branch through a four-way joint, and the first branch, the second branch and the third branch are respectively provided with a flow stopping clamp; the storage box is provided with a box door, the top of the storage box is provided with three holes, a transfer bag is arranged in the storage box, the transfer bag is divided into a first chamber, a second chamber and a third chamber from left to right through a heat seal bag inner line, and the first branch, the second branch and the third branch correspondingly penetrate through the three holes in sequence and are communicated with the first chamber, the second chamber and the third chamber respectively.
3. The utility model provides an umbilical cord mesenchymal stem cell separation extraction element (CN206680476U), includes quick-witted case, backing plate and end cover, the backing plate welding is at the quick-witted roof portion, quick-witted case and backing plate mid-weld have the lantern ring, the end cover is installed in backing plate one side, through hinge connection between end cover and the backing plate, the motor is installed to quick-witted incasement side bottom, machine incasement side mid-mounting has the acoustic celotex board, install the pivot on the motor, the pivot upper end runs through the acoustic celotex board and installs the carousel, install the test tube seat on the carousel, be provided with circular through-hole in the test tube seat, the welding of pivot top has the thimble, the end cover inboard is fixed with the rubber pad, the rubber pad is the round platform shape, the rubber pad mid.
Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome prior art's weak point, provide a human source umbilical cord mesenchymal stem cells separator, the device structure as an organic whole, not only simple structure, washing convenience can also guarantee to cut garrulous tissue piece size suitable, cut labour saving and time saving, comfortable operation.
The utility model provides a its technical problem take following technical scheme to realize:
a separation device for human umbilical cord mesenchymal stem cells comprises a separation box body, wherein the right side of the separation box body is a cleaning area, the left side of the separation box body is provided with a separation area, a shearing area and an appliance storage area, three stainless steel clapboards are uniformly and fixedly arranged in the cleaning area at intervals, the cleaning area is divided into a first cleaning box, a second cleaning box, a third cleaning box and a fourth cleaning box by the stainless steel clapboards, the middle part of each stainless steel clapboard is provided with a movable valve plate, and the outer side wall of the first cleaning box is provided with a water outlet with a valve; the separation area is used for separating umbilical cord and vein, and cutting the umbilical cord into rectangular umbilical cord tissues; telescopic rods are arranged at four corners in the shearing area, a metal hook is arranged at the tail end of each telescopic rod, the metal hooks are used for flatly paving and hanging the rectangular umbilical cord tissues, and a rectangular opening is formed in the left side of the shearing area; the instruments placed in the instrument storage area comprise scissors, hemostatic forceps, umbilical cord forceps and a crochet hook.
And, the umbilical cord pincers include tool bit, lower tool bit, go up handle and lower handle, go up the handle and pass through the pin rotation with lower handle and be connected, lower tool bit integrated into one piece is at last handle of a knife front end, and lower tool bit integrated into one piece is at handle of a knife front end down, and the terminal surface that goes up the tool bit and be the face-like structure with lower tool bit is relative, lower tool bit upper surface system has the perpendicular alternate cutting edge of violently erectting, forms a cutting edge grid groove, upper tool bit lower surface system has the square projection cutting edge with the cutting edge grid groove looks adaptation of lower tool bit.
And a return spring is arranged between the upper handle and the lower handle.
And rubber is arranged on the upper handle and the lower handle, and anti-skid pads are arranged on the rubber.
And the front ends of the upper cutter head and the lower cutter head are blunt heads.
Moreover, the bottom surface of the cleaning area is obliquely arranged, and the included angle between the bottom surface and the horizontal plane is 5-10 degrees.
The utility model has the advantages that:
1. the utility model relates to an integrative stainless steel material makes, simple structure, easy washing, autoclaving etc. compare with traditional separation way, need not to prepare a plurality of beakers, and the operation is more convenient, comfortable, labour saving and time saving, can increase of service life moreover.
2. The utility model discloses the district is deposited to the utensil that sets up, and the utensil integration of depositing with the dispersion is deposited and is deposited in together, has not only simplified the parking space, has improved work efficiency moreover greatly.
3. The utility model discloses cut garrulous last tool bit that adopts umbilical cord pincers and terminal surface relative with lower tool bit of adopting is surface-shaped structure, lower tool bit upper surface system has the perpendicular alternate cutting edge of anyhow, forms a cutting edge grid groove, go up tool bit lower surface system have with the square projection cutting edge of the cutting edge grid groove looks adaptation of lower tool bit for incision lifting surface area is big, is favorable to the cutting garrulous of navel cord, but the navel cord is cut garrulous in-process anti-drop to shorten the navel cord and cut garrulous time, improve work efficiency.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of the umbilical cord forceps of the present invention;
FIG. 3 is a schematic structural view of the upper tool bit of the umbilical clamp of the present invention;
fig. 4 is a schematic structural view of the lower cutter head of the umbilical clamp of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.
A separation device for human umbilical cord mesenchymal stem cells comprises a separation box body 14, wherein the separation box body is a cuboid box body which is made of stainless steel and is provided with an opening at the upper part, and the internal space of the separation box body is partitioned by a stainless steel partition plate to form a cleaning area 1, a separation area 15, a shearing area 10 and an appliance storage area 9; the washing district is located the right side of separation box body, disengagement zone, shearing district and utensil deposit the district and evenly arrange respectively and set up the left side at the separation box body, the washing district is washed the navel cord with normal saline to the delivery port in the washing box outside through the washing district is with waste water discharge separation box body, peels off quiet, the artery in the navel cord in the disengagement zone, and takes to arrange the scissors of utensil deposit the district in and cut the navel cord, and the navel cord level that will cut out is spread out and is hung to shearing the district in, takes to arrange the quick even shearing of the navel cord piece of cutting the district in the navel cord pincers 8 of utensil deposit the district in.
Three stainless steel clapboards 4 are fixedly arranged in the cleaning area at intervals, the cleaning area is divided into a first cleaning box 2, a second cleaning box 5, a third cleaning box 6 and a fourth cleaning box 7 by the stainless steel clapboards, in order to facilitate the filling and discharging of cleaning water in the cleaning area, a movable valve plate 3 capable of being pushed and pulled upwards is arranged in the middle of each stainless steel clapboard, a water outlet is formed in the box wall on the outer side of each cleaning box, a valve 16 is correspondingly arranged on each water outlet, in order to enable waste water in the cleaning area to be better discharged, the bottom surface of the cleaning area is obliquely arranged, the inclined included angle between the bottom surface and the horizontal plane is 5-10 degrees, after the umbilical cord cleaning is finished, each movable valve plate is pulled upwards, the valve of the water outlet is opened, and the waste water can flow out of the separating box body.
The separation area is used for stripping the artery and the vein in the umbilical cord and shearing the stripped umbilical cord into a rectangular structure with the length of about 5cm and the width of about 4 cm.
Four angles all can be dismantled in the shearing area and install telescopic link 13, metal hook 12 of installation all can be dismantled to every telescopic link end, the length of interval is about 4.5cm between four couples, the width is about 3.5cm, slightly less than the size of navel cord, there is a rectangle opening shearing district left side system integratively, the navel cord pincers of being convenient for enter into and cut out, during the operation, the four corners of the rectangle navel cord that will handle are respectively hooked on four metal hook, spread out rectangle navel cord level, get into the shearing district with the navel cord pincers through the rectangle opening and cut, make things convenient for the use of navel cord pincers greatly, if there is the tissue piece to be stained with the supporting crochet hook in the use device when gluing on the tool bit to take off after the shearing.
The umbilical cord forceps comprise an upper cutter head 18, a lower cutter head 20, an upper handle 22 and a lower handle 25, wherein the upper handle is rotatably connected with the lower handle through a pin 21, the lower cutter head is integrally formed at the front end of the upper handle, the lower cutter head is integrally formed at the front end of the lower handle, the opposite end surfaces of the upper cutter head and the lower cutter head are of a planar structure, the upper cutter head and the lower cutter head are made of stainless steel materials, the upper surface of the lower cutter head is provided with transverse blades 26 and vertical blades 27, the transverse blades and the vertical blades are vertically alternated, the distance between every two blades is 3mm, a blade grid groove 19 is formed, the lower surface of the upper cutter head is provided with square convex column blades 17 matched with the blade grid groove of the lower cutter head, so that the stress area of a cut is large, the cutting of an umbilical cord is facilitated, the umbilical cord can be prevented from falling off in the cutting process, the cutting time of the umbilical cord is shortened, the working efficiency is, the cutting edge of the square convex column of the upper cutter head is just embedded in the grid groove of the lower cutter head, and umbilical cord tissue sheets between the upper cutter head and the lower cutter head are cut into small tissue sheets with the same size and the side length of 3mm by the cutting edges which are vertically arranged at intervals.
A return spring 24 is arranged between the upper handle and the lower handle and is positioned between the two handles, the upper handle and the lower handle can return quickly through the arrangement of the return spring, in addition, the upper handle and the lower handle are limited by the compressed return spring, and the maximum opening degree between the upper cutter head and the lower cutter head is 45 degrees; all be provided with rubber on two handles, be provided with slipmat 23 on the rubber, increased the frictional force between user and the handle, reduced the risk that exists in the use, safe and reliable, when using this umbilical cord pincers, for the ease of contact of umbilical cord pincers, the front end of going up tool bit, lower tool bit is blunt, but the one hand utilizes umbilical cord pincers directly to cut into pieces the umbilical cord in cutting into pieces the district.
The device storage area can be used for placing devices such as scissors, hemostatic forceps, umbilical cord forceps and a crochet hook, the devices are convenient to take and store, the crochet hook is stored in the device storage area, and the crochet hook is convenient to take down if tissue blocks are adhered to the cutter head after being cut.
The utility model discloses a stainless steel material as an organic whole makes, and the cleaning and disinfection of being convenient for compares more conveniently with the way that a plurality of beakers were prepared to the tradition, and labour saving and time saving can increase of service life moreover.
The utility model discloses a beaker is compared to interior height and more is fit for the operation, the umbilical cord of being convenient for washs and operation such as shearing.
The utility model discloses an umbilical cord pincers can convenient and fast's the shearing of completion umbilical cord, and the tissue size of shearing is the same for experiment labour saving and time saving more is good foundation for the umbilical cord mesenchymal stem cell culture in later stage, and has improved the efficiency of experiment greatly.
Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.
Claims (6)
1. The utility model provides a human umbilical cord mesenchymal stem cell separator which characterized in that: the separation box comprises a separation box body, wherein a cleaning area is arranged on the right side of the separation box body, a separation area, a shearing area and an appliance storage area are arranged on the left side of the separation box body, three stainless steel partition plates are uniformly and fixedly arranged in the cleaning area at intervals, the cleaning area is divided into a first cleaning box, a second cleaning box, a third cleaning box and a fourth cleaning box by the stainless steel partition plates, a movable valve plate is arranged in the middle of each stainless steel partition plate, and a water outlet with a valve is formed in the outer side wall of the first cleaning box; the separation area is used for separating umbilical cord and vein, and cutting the umbilical cord into rectangular umbilical cord tissues; telescopic rods are arranged at four corners in the shearing area, a metal hook is arranged at the tail end of each telescopic rod, the metal hooks are used for flatly paving and hanging the rectangular umbilical cord tissues, and a rectangular opening is formed in the left side of the shearing area; the instruments placed in the instrument storage area comprise scissors, hemostatic forceps, umbilical cord forceps and a crochet hook.
2. The human umbilical cord mesenchymal stem cell separation device of claim 1, wherein: umbilical cord pincers include tool bit, lower tool bit, go up handle and lower handle, go up the handle and pass through the pin rotation with lower handle and be connected, lower tool bit integrated into one piece is at last handle of a knife front end, and lower tool bit integrated into one piece is at handle of a knife front end down, and the terminal surface that goes up the tool bit and be the face-like structure with lower tool bit is relative, lower tool bit upper surface system has the perpendicular alternate cutting edge of violently erectting, forms a cutting edge grid groove, upper tool bit lower surface system has the square projection cutting edge with the cutting edge grid groove looks adaptation of lower tool bit.
3. The human umbilical cord mesenchymal stem cell separation device of claim 2, wherein: and a return spring is also arranged between the upper handle and the lower handle.
4. The human umbilical cord mesenchymal stem cell separation device of claim 2, wherein: rubber is arranged on the upper handle and the lower handle, and anti-slip pads are arranged on the rubber.
5. The human umbilical cord mesenchymal stem cell separation device of claim 2, wherein: the front ends of the upper cutter head and the lower cutter head are blunt heads.
6. The human umbilical cord mesenchymal stem cell separation device of claim 1, wherein: the bottom surface of the cleaning area is obliquely arranged, and the included angle between the bottom surface and the horizontal plane is 5-10 degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920563548.5U CN210127228U (en) | 2019-04-24 | 2019-04-24 | Human umbilical cord mesenchymal stem cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920563548.5U CN210127228U (en) | 2019-04-24 | 2019-04-24 | Human umbilical cord mesenchymal stem cell separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210127228U true CN210127228U (en) | 2020-03-06 |
Family
ID=69661925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920563548.5U Expired - Fee Related CN210127228U (en) | 2019-04-24 | 2019-04-24 | Human umbilical cord mesenchymal stem cell separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210127228U (en) |
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2019
- 2019-04-24 CN CN201920563548.5U patent/CN210127228U/en not_active Expired - Fee Related
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