CN210065790U - Reactor for producing theaflavin by using immobilized polyphenol oxidase - Google Patents
Reactor for producing theaflavin by using immobilized polyphenol oxidase Download PDFInfo
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- CN210065790U CN210065790U CN201822145093.3U CN201822145093U CN210065790U CN 210065790 U CN210065790 U CN 210065790U CN 201822145093 U CN201822145093 U CN 201822145093U CN 210065790 U CN210065790 U CN 210065790U
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Abstract
The utility model discloses an utilize reactor of immobilized polyphenol oxidase production theaflavin, include the reaction column and join in marriage the fluid reservoir, the reaction column embeds there is the immobilized enzyme, and the reaction column top is equipped with inlet and gas outlet respectively, and the reaction column bottom is equipped with air inlet and liquid outlet respectively, the inlet still is equipped with the circulating pump with joining in marriage on the pipeline between the fluid reservoir, bottom in the reaction column still is equipped with air distributor. The utility model adopts a liquid inlet mode from top to bottom and a gas inlet mode from bottom to top, and simultaneously, the reaction liquid is repeatedly and circularly reacted for many times, thereby effectively improving the reaction efficiency and the reaction completeness, and improving the yield and the quality of the theaflavin product generated by the reaction; in addition, the air distributor enables air entering the reaction column to form uniform airflow, and further promotes the reaction to be fully carried out; the utility model has the advantages of simple and reasonable design, energy-concerving and environment-protective, have good economy and social, be favorable to promoting and popularizing.
Description
Technical Field
The utility model relates to a theaflavin production facility field, concretely relates to utilize reactor of immobilized polyphenol oxidase production theaflavin.
Background
The theaflavin is an important quality component and a functional component in the black tea, and has various biological activities and pharmacological effects of reducing blood fat, reducing blood pressure, resisting oxidation, radiation, bacteria, cancer and the like, so the theaflavin has good medical health-care function. Theaflavin is a chemical component with the minimum molecular weight, the simplest structure and the most definite biological activity in tea pigment series products, and is the only tea active component with definite pharmacological action.
Theaflavin is prepared by reacting tea polyphenols and oxygen under the catalysis of polyphenol oxidase to generate o-quinone, further oxidizing o-quinone to obtain intermediate product of diphenol quinone, reducing one part of diphenol quinone to obtain flavanol, and further oxidizing the other part of diphenol quinone under the catalysis of polyphenol oxidase to obtain theaflavin. Theaflavins were first extracted directly from black tea, however black tea itself contained only 0.5-2.0% theaflavins, resulting in a lower extraction yield. At present, the theaflavin is industrially produced mainly by adopting a tea polyphenol enzymatic oxidation method, crushing fresh tea leaves, putting the crushed fresh tea leaves into a reaction kettle, adding a solvent and tea polyphenol, introducing air for reaction, and carrying out enzymatic synthesis by utilizing polyphenol oxidase naturally existing in the fresh tea leaves to obtain the theaflavin. However, the method has high industrial cost due to low content of polyphenol oxidase in tea, and the polyphenol oxidase has low utilization efficiency, so that the reaction is difficult to be fully performed, and substances such as polysaccharide, caffeine and the like in fresh tea can also cause adverse effects on the quality of theaflavin products.
SUMMERY OF THE UTILITY MODEL
To the technical problem who exists, the utility model provides an utilize immobilized polyphenol oxidase to produce theaflavin's reactor has not only improved polyphenol oxidase's in-process of theaflavin utilization ratio, has effectively improved reaction efficiency and reaction completeness moreover, has improved theaflavin's yield and quality.
The utility model provides a technical scheme that its technical problem adopted is: the utility model provides an utilize reactor of immobilized polyphenol oxidase production theaflavin, includes the reaction column and joins in marriage the fluid reservoir, the reaction column embeds there is the immobilized enzyme, and the reaction column top is equipped with inlet and gas outlet respectively, and the reaction column bottom is equipped with air inlet and liquid outlet respectively, inlet and liquid outlet link to each other with joining in marriage the fluid reservoir through the pipeline respectively, the inlet still is equipped with the circulating pump with joining in marriage on the pipeline between the fluid reservoir, bottom in the reaction column still is equipped with air distributor, air distributor locates the immobilized enzyme below, and is located air inlet and liquid outlet top.
Further, the ratio of the diameter to the height of the reaction column is 1: 12.
Further, the air outlet is connected with a condenser.
The utility model has the advantages that: the utility model takes immobilized polyphenol oxidase as a reaction catalyst, adopts a liquid inlet mode from top to bottom and an air inlet mode from bottom to top, effectively increases the contact area among air, tea polyphenol reaction liquid and immobilized enzyme, simultaneously leads the reaction liquid to carry out repeated cyclic reaction for many times, effectively improves the reaction efficiency and the reaction completeness, and promotes the yield and the quality of the theaflavin product generated by the reaction; in addition, the air distributor enables air entering the reaction column to form uniform airflow, and the contact area of the air with the immobilized enzyme and the tea polyphenol reaction liquid is further increased, so that the reaction is further promoted to be fully performed; the utility model has the advantages of simple and reasonable design, energy-concerving and environment-protective, have good economy and social, be favorable to promoting and popularizing.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
in the figure: 1-reaction column, 11-liquid inlet, 12-liquid outlet, 13-air inlet, 14-air outlet, 2-liquid preparation tank, 3-circulating pump, 4-air distributor and 5-condenser.
Detailed Description
The present invention will now be described in detail with reference to the accompanying drawings and examples.
The utility model provides an utilize reactor of immobilized polyphenol oxidase production theaflavin, includes reaction column 1 and joins in marriage fluid reservoir 2, reaction column 1 embeds there is the immobilized enzyme, and 1 top of reaction column is equipped with inlet 11 and gas outlet 14 respectively, and 1 bottom of reaction column is equipped with air inlet 13 and liquid outlet 12 respectively, inlet 11 and liquid outlet 12 link to each other with joining in marriage fluid reservoir 2 through the pipeline respectively, still be equipped with circulating pump 3 on inlet 11 and the pipeline of joining in marriage between the fluid reservoir 2, bottom in the reaction column 1 still is equipped with air distributor 4, immobilized enzyme below is located to air distributor 4, and is located air inlet 13 and liquid outlet 12 top.
Further, the ratio of the diameter to the height of the reaction column 1 was 1: 12.
Further, the air outlet 14 is connected with a condenser 5, and the condenser 5 is used for recovering the ethyl acetate solvent carried in the air discharged from the air outlet 14.
The utility model discloses a concrete theory of operation as follows: firstly, tea polyphenol and ethyl acetate are sequentially added into a liquid preparation tank 2 to prepare tea polyphenol reaction liquid with a certain concentration, the tea polyphenol reaction liquid is introduced into a reaction column 1 from top to bottom through a liquid inlet 11 at the top end of the reaction column 1 by a circulating pump 3, meanwhile, air is introduced into the reaction column 1 from an air inlet 13 at the bottom end of the reaction column 1 through an air distributor 4 from bottom to top, so that the air, the tea polyphenol reaction liquid and immobilized enzyme are ensured to be in full contact reaction, then the reaction liquid enters the liquid preparation tank 2 through a liquid outlet 12 and a pipeline in sequence, and then enters the reaction column 1 from the liquid inlet 11 again through the circulating pump 3 for repeated reaction, the reaction is finished after the circulation is repeated for many times, and finally, a theaflavin product is obtained through post.
The utility model adopts a liquid inlet mode from top to bottom and a gas inlet mode from bottom to top, which effectively increases the contact area among air, tea polyphenol reaction liquid and immobilized enzyme, and simultaneously, utilizes a circulating pump and a liquid preparation tank to make the reaction liquid carry out repeated circulating reaction for a plurality of times, effectively improves the reaction efficiency and the reaction completeness, and promotes the yield and the quality of the theaflavin product generated by the reaction; in addition, the air distributor enables air entering the reaction column to form uniform airflow, and further increases the contact area of the air with the immobilized enzyme and the tea polyphenol reaction liquid, so as to further promote the reaction to be fully carried out.
The above embodiments are merely illustrative of the scope of the present invention, and all equivalent changes and modifications made according to the spirit of the present invention should be covered by the scope of the present invention.
Claims (3)
1. A reactor for producing theaflavin by utilizing immobilized polyphenol oxidase comprises a reaction column (1) and a liquid preparation tank (2), and is characterized in that: reaction column (1) embeds there is the immobilized enzyme, and reaction column (1) top is equipped with inlet (11) and gas outlet (14) respectively, and reaction column (1) bottom is equipped with air inlet (13) and liquid outlet (12) respectively, inlet (11) and liquid outlet (12) link to each other with joining in marriage fluid reservoir (2) through the pipeline respectively, still be equipped with circulating pump (3) on inlet (11) and the pipeline of joining in marriage between fluid reservoir (2), bottom in reaction column (1) still is equipped with air distributor (4), the immobilized enzyme below is located in air distributor (4), and is located air inlet (13) and liquid outlet (12) top.
2. The reactor for producing theaflavins using immobilized polyphenol oxidase of claim 1, wherein: the ratio of the diameter to the height of the reaction column (1) is 1: 12.
3. The reactor for producing theaflavins using immobilized polyphenol oxidase of claim 1, wherein: the air outlet (14) is connected with a condenser (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822145093.3U CN210065790U (en) | 2018-12-19 | 2018-12-19 | Reactor for producing theaflavin by using immobilized polyphenol oxidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822145093.3U CN210065790U (en) | 2018-12-19 | 2018-12-19 | Reactor for producing theaflavin by using immobilized polyphenol oxidase |
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| Publication Number | Publication Date |
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| CN210065790U true CN210065790U (en) | 2020-02-14 |
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| CN201822145093.3U Active CN210065790U (en) | 2018-12-19 | 2018-12-19 | Reactor for producing theaflavin by using immobilized polyphenol oxidase |
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2018
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