CN210037621U - A device for yellow dragon disease detects - Google Patents
A device for yellow dragon disease detects Download PDFInfo
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- CN210037621U CN210037621U CN201920491635.4U CN201920491635U CN210037621U CN 210037621 U CN210037621 U CN 210037621U CN 201920491635 U CN201920491635 U CN 201920491635U CN 210037621 U CN210037621 U CN 210037621U
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Abstract
The utility model discloses a device for yellow dragon disease detects, including test paper and test part, the test paper has bellied water-fast test paper for the surface, test part is including detection container and dropwise add part, dropwise add part includes the button, spacing part, the piston rod, a piston, the drip chamber, drip pipe and stock solution container, the one end of piston rod links to each other with the button, the other end links to each other with the piston, spacing part overlaps on the piston rod just, piston rod and piston stretch into the drip chamber in, the setting is pasted mutually with the lateral wall in drip chamber to the piston, stock solution container is connected with the drip chamber, drip pipe one end stretches into in the stock solution container, the other end stretches into in the detection container. In this way, the utility model discloses utilize abrasive paper as the carrier, scrape the mesophyll tissue of the blade of detection object through the friction blade and adhere to on the abrasive paper surface, detect this abrasive paper again, and do not detect with the blade, this kind of method is more economical and practical, and speed is faster moreover, and efficiency is higher.
Description
Technical Field
The utility model relates to a plant disease detection area especially relates to a device that is used for yellow dragon to detect.
Background
The yellow shoot is also called citrus yellow shoot, and is a disease with extremely wide range and great harm. The pathogenic bacteria of the yellow dragon disease are special parasitic phloem bacteria of the citrus, and the bacteria infect the phloem and the root system of the citrus to cause the trees to be weak and dead. The Huanglongbing disease usually has a long onset period of 1-3 years. The infected citrus trees have mottled yellow leaves and do not or have inferior fruits.
Yellowing of citrus leaves alone cannot be accurately judged because citrus lacks nutrient elements and can cause yellowing of the leaves due to nematode infection or citrus tristeza. If the yellow dragon disease is judged by simply relying on the yellowing of the leaves, the misjudgment is often caused. On the other hand, the degree of the onset of huanglongbing cannot be determined simply by observing the yellow color of the leaves. Because the degree of the yellow shoot disease is related to the number of colonies of the tree body and the physiological state of the citrus tree body, and the shade of the yellow color of the leaf is not linearly related to the factors.
There are many researchers who have studied on these technical problems, including some patents. For example, patent CN205861561U, "a portable citrus huanglongbing detection device" uses an infrared camera to detect the degree of huanglongbing, and its accuracy is questionable. CN 201410744410.7A rapid screening method for citrus greening disease prevention and control medicaments adopts a PCR method for detection. Although highly accurate, this is not a method that can be applied in the field on a large scale. CN201310244391.7, "a rapid diagnosis method for citrus huanglongbing", and CN201410326822.9, "a rapid detection method for citrus huanglongbing", provide a rapid detection method for huanglongbing, but the operation is complicated, the method comprises the steps of firstly collecting leaves suspected to have huanglongbing, polishing the surfaces of the leaves with sand paper, then putting the leaves into a solution with chlorophyll removed, and then adding a color development solution for detecting starch for color development. These steps are cumbersome, involve additional freezing of the leaves, and are time consuming in each step, consuming a lot of reagents. Is not suitable for being used in the field quickly and has higher cost.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the main technical problem who solves provides a device for yellow dragon disease detects, and the device is simple and efficient, has practicality and economic nature, is applicable to the field to yellow dragon disease's short-term test.
In order to solve the technical problem, the utility model discloses a technical scheme be: provides a device for detecting the huanglongbing, which comprises test paper and a detection part, wherein the test paper is water-resistant test paper with bulges on the surface, the detection part comprises a detection container and a dropping part, the dropping part comprises a button, a limiting part, a piston rod, a piston, a dropping liquid cavity, a dropping liquid pipe and a liquid storage container, one end of the piston rod is connected with the button, the other end of the piston rod is connected with the piston, the limiting component is sleeved on the piston rod and is positioned between the button and the liquid dropping cavity, the piston rod and the piston extend into the liquid dropping cavity, the piston is attached to the side wall of the liquid dropping cavity, the liquid storage container is positioned at the lower part of the liquid dropping cavity and is connected with the liquid dropping cavity, one end of the liquid dropping pipe extends into the liquid storage container, the other end of the test paper extends into the detection container, and the test paper is positioned in the detection container during detection.
In a preferred embodiment of the present invention, the test paper is a strip-shaped waterproof sand paper with a frosted surface.
In a preferred embodiment of the present invention, the detection container is a transparent container with an opening at the top and capable of being closed.
In a preferred embodiment of the present invention, the detection container is a transparent plastic self-sealing bag.
In a preferred embodiment of the present invention, the position-limiting member is a fixed spring.
In a preferred embodiment of the present invention, one end of the fixing spring is fixed to a top edge of the drip chamber.
In a preferred embodiment of the present invention, the apparatus for yellow dragon disease detection further comprises a camera, and the camera is located around the detection container.
The utility model has the advantages that: the utility model discloses a device for yellow dragon disease detects is to get rid of the wax layer on the blade with abrasive paper among the prior art, and this application utilizes abrasive paper as the carrier, scrapes off the mesophyll tissue of the blade of detection object through the friction blade and adheres to on the abrasive paper surface, detects this abrasive paper again, and does not detect with the blade, and this kind of method is more economical and practical, and speed is faster moreover, and efficiency is higher.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive work, wherein:
FIG. 1 is a schematic structural diagram of a preferred embodiment of the apparatus for yellow dragon disease detection according to the present invention;
fig. 2 is a schematic structural diagram of a test paper in the device for detecting huanglongbing shown in fig. 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1 and 2, a device for detecting huanglongbing is provided, which includes a test paper 1 and a detecting member. The test paper 1 is water-resistant test paper with a bulge on the surface, and the test paper can be selected to be strip-shaped water-resistant sand paper with frosted surfaces 2. A camera 4 is arranged in the device for yellow dragon disease detection, and the camera 4 is positioned around the detection container. The device for detecting the huanglongbing can comprise a connecting frame for connecting all the parts, and the camera 4 can be arranged on the connecting frame. The camera 4 can shoot every sample and store data, records the testing process, leaves the evidence.
The detection part comprises a detection container and a dropping part. The detection container is a transparent container with an opening at the top and capable of being sealed, and can be a bag or a bottle. The detection container comprises a transparent inner part 11 and a seal 12 which can be freely opened and closed, wherein the seal 12 is positioned at the upper part of the inner part 11 and is connected with the inner part. The test paper 1 is put into the detection container in the detection process. The transparent detection container can clearly see the change of the test paper in the container, so that the detection result can be conveniently known.
The dropping component comprises a button 5, a limiting component, a piston rod 6, a piston 7, a dropping liquid cavity 8, a dropping liquid pipe 9 and a liquid storage container 10. One end of the piston rod 6 is connected with the button 5, and the other end is connected with the piston 7, the connection is fixed, for example, the end of the piston rod 6 can be glued with the button 5 together, or the button 5, the piston rod 6 and the piston 7 are integrally formed by casting. The limiting component is a solid spring 3, and the fixed spring 3 is sleeved on the piston rod 6 and is positioned between the button 5 and the liquid dropping cavity 8. One end of the fixing spring 3 is fixed on the top edge of the drip chamber 8, and can be fixed in an adhesive way. The piston rod 6 and the piston 7 extend into the liquid dropping cavity 8, and the piston 7 is attached to the side wall of the liquid dropping cavity 8. The liquid storage container 10 is located at the lower part of the liquid dropping cavity 8 and connected with the liquid dropping cavity 8, and a detection reagent is filled in the liquid storage container 10. One end of the dropping liquid pipe 9 extends into the liquid storage container 10, and the other end of the dropping liquid pipe extends into the detection container. The dropping liquid pipe 9 is a soft hollow pipe, can be bent, and can allow the detection reagent to normally flow.
The design enables the lower surface of the piston 7, the side wall of the dropping liquid cavity 8 and the liquid level of the detection reagent in the liquid storage container 10 to form a closed cavity. When the button is pressed down, the piston rod 6 drives the piston 7 to be pressed down, so that pressure is generated in the closed cavity, and a detection reagent in the liquid storage container 10 is pressed into the liquid dropping pipe 9 under the action of the pressure, so that the effect of liquid dropping is achieved. The fixed spring 3 defines the depression distance of the push-button 5, thus fixing the volume of depression of the piston 7 and enabling the return thereof when the push-button 5 is not stressed.
The operation process of the device for detecting the huanglongbing comprises the following steps: the liquid storage container 10 contains a liquid detection reagent. When the button 5 is pressed, the button 5 depresses the piston rod 6 pushing the piston 7 down in the drip chamber 8. The piston 7 compresses the air to generate pressure. The resulting pressure causes a volume of test agent contained in a reservoir 10 to drip along a drip tube 9 into the interior 11 of the test container. The fixed spring 3 defines the depression distance of the button 5, thus defining the volume of the drip, and enables the button 5 to be reset when it is not stressed, enabling continuous drip.
When identifying the huanglongbing, the frosted surface of the test paper is firstly used for rubbing the suspected huanglongbing leaves to be tested for 30 times, and the force is based on that the leaves are not worn. After rubbing, the mesophyll tissue and material of the leaf were obtained on the test paper. The test strip is then placed into the interior 11 of the test container. The dripping component is used for adding a detection reagent into the interior 11 of the detection container to perform color reaction so as to identify the occurrence degree of the huanglongbing. The detection reagent can be selected according to different detection methods, such as a starch detection reagent, a copper sulfate discoloration detection reagent and the like.
The first implementation example:
the waterproof sand paper was cut into strips of 2 cm by 1 cm as test paper. Leaves of suspected Huanglongbing trees are collected in the field. The surface of the blade is rubbed by the sanding surface of the sand paper about 30 times, and the force is based on not wearing the blade. After the rubbing is finished, mesophyll tissues and substances are adhered to the surface of the sand paper. The sandpaper was placed in a small sized valve bag (5 cm by 8 cm in size). The self-sealing bag is a transparent detection container. After that the button 5 is pressed, the button 5 presses the piston 7 through the piston rod 6, and the piston 7 generates air pressure in the drip chamber 8. Under the action of generated air pressure, 2 ml of starch detection reagent and 2 ml of auxiliary detection reagent are respectively added into a self-sealing bag containing abrasive paper from a left liquid storage container 10 and a right liquid storage container 10 through a dropping liquid pipe 9, a mouth is sealed, and the reagents and the abrasive paper are fully and uniformly mixed by hands. Leaves with yellow dragon disease will show a characteristic dark black color. The result can be photographed and stored by the camera 4. When the button 5 is released, the button 5 returns to the original position under the action of the fixed spring 3.
Example two:
the waterproof sand paper was cut into strips of 2 cm by 1 cm as test paper. Leaves of suspected Huanglongbing trees are collected in the field. The surface of the blade is rubbed by the sanding surface of the sand paper about 30 times, and the force is based on not wearing the blade. After the rubbing is finished, mesophyll tissues and substances are adhered to the surface of the sand paper. The sandpaper was placed in a small sized valve bag (5 cm by 8 cm in size). The self-sealing bag is a transparent detection container. After that the button 5 is pressed, the button 5 presses the piston 7 through the piston rod 6, and the piston 7 generates air pressure in the drip chamber 8. Under the action of generated air pressure, 2 ml of copper sulfate detection reagent and 2 ml of auxiliary detection reagent are respectively added into a self-sealing bag containing abrasive paper from a left liquid storage container 10 and a right liquid storage container 10 through a dropping liquid pipe 9, a mouth is sealed, and the reagents and the abrasive paper are fully and uniformly mixed by hands. Leaves with yellow dragon disease will show a characteristic dark green color. The result can be photographed and stored by the camera 4. When the button 5 is released, the button 5 returns to the original position under the action of the fixed spring 3.
Example three was carried out:
the waterproof sand paper was cut into strips of 2 cm by 1 cm as test paper. Leaves of suspected Huanglongbing trees are collected in the field. The surface of the blade is rubbed by the sanding surface of the sand paper about 30 times, and the force is based on not wearing the blade. After the rubbing is finished, mesophyll tissues and substances are adhered to the surface of the sand paper. The sandpaper was placed in a small size clear capped bottle. The self-sealing bag is a transparent detection container. The button 5 is then pressed, the button 5 depressing the piston 7 via the piston rod 6. The piston 7 generates air pressure in the drip chamber 8. Under the effect of the generated air pressure, 2 ml of starch detection reagent and 5 ml of auxiliary detection reagent are respectively added into a plastic bottle containing abrasive paper from a left liquid storage container 10 and a right liquid storage container 10 through a left liquid dropping pipe 9 and a right liquid dropping pipe 9, a cover is covered, and the reagents and the abrasive paper are fully mixed uniformly by vibration. Leaves with yellow dragon disease will show a characteristic dark black color. The result can be photographed and stored by the camera 4.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all of which utilize the equivalent structure or equivalent flow transformation made by the content of the specification of the present invention, or directly or indirectly applied to other related technical fields, all included in the same way in the patent protection scope of the present invention.
Claims (7)
1. A device for detecting huanglongbing is characterized by comprising test paper and a detection part, the test paper is water-resistant test paper with bulges on the surface, the detection part comprises a detection container and a dropping part, the dropping component comprises a button, a limiting component, a piston rod, a piston, a dropping liquid cavity, a dropping liquid pipe and a liquid storage container, one end of the piston rod is connected with the button, the other end of the piston rod is connected with the piston, the limiting component is sleeved on the piston rod and is positioned between the button and the liquid dropping cavity, the piston rod and the piston extend into the liquid dropping cavity, the piston is attached to the side wall of the liquid dropping cavity, the liquid storage container is positioned at the lower part of the liquid dropping cavity and is connected with the liquid dropping cavity, one end of the liquid dropping pipe extends into the liquid storage container, the other end of the test paper extends into the detection container, and the test paper is positioned in the detection container during detection.
2. The device for huanglongbing as claimed in claim 1, wherein said strip of waterproof sand paper is sanded on one side.
3. The apparatus for huanglongbing as claimed in claim 1, wherein said detection container is a transparent container with an opening at the top and capable of being closed.
4. The apparatus of claim 1, wherein the detection vessel is a transparent plastic self-sealing bag.
5. The device for huanglongbing as claimed in claim 1, wherein said position-limiting member is a fixed spring.
6. The device for huanglongbing detection of claim 5, wherein one end of said securing spring is secured to a top edge of said drip chamber.
7. The apparatus for huanglongbing as claimed in claim 1, further comprising a camera positioned around the detection container.
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CN117268864A (en) * | 2022-10-18 | 2023-12-22 | 衢州学院 | Be used for Hu Youhuang dragon disease field batch detection device |
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CN117268864A (en) * | 2022-10-18 | 2023-12-22 | 衢州学院 | Be used for Hu Youhuang dragon disease field batch detection device |
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Effective date of registration: 20220121 Address after: 336000 pharmaceutical industry park, Yuanzhou District, Jiangxi, Yichun Patentee after: JIANGXI NEW DRAGON BIOTECHNOLOGY CO.,LTD. Address before: 336000 No. 311 Yichun North Road, Yuanzhou District, Yichun City, Jiangxi Province Patentee before: Deng Zhengan Patentee before: Deng Fangkun Patentee before: JIANGXI NEW DRAGON BIOTECHNOLOGY CO.,LTD. |