CN209996527U - Mouse model intrasplenic injection fixing device - Google Patents

Abstract

The utility model discloses an mouse model splenic injection fixing device, including the mouse model fixed station, the preceding lateral wall middle part of mouse model fixed station has vertically seted up logical groove, with lead to the recessed formation mouse body support groove in the corresponding mouse model fixed station upper surface middle part of groove position, lead to and seted up the forelimb fixed slot on the left mouse model fixed station of groove, it has seted up hind limb fixed slot and mouse tail fixed slot to lead to the mouse model fixed station on groove right side, it has all spanned the magic sticky tape on preceding, hind limb fixed slot and the mouse tail fixed slot, the end of magic sticky tape links firmly with the mouse model fixed station mutually, it is in addition end and bonding piece activity bonding, the medial surface middle part of magic sticky tape is provided with elasticity and ties up the ring, the angle department of mouse model fixed station is provided with metal forming hose, the upper end of metal forming hose has the light through universal bulb hinge, be provided with charging source in the mouse model fixed station.

Description

Mouse model intrasplenic injection fixing device

Technical Field

The utility model belongs to the technical field of biological immunity experiment technique and specifically relates to an injection fixing device in mouse model spleen.

Background

The spleen is the largest immune organ of the body, accounts for 25 percent of the total amount of the systemic lymphoid tissue, contains a large amount of lymphocytes and macrophages, and is the center of cellular immunity and humoral immunity of the body. The adoption of an intraperitoneal injection method for carrying out basic immunization and non-secondary tail vein injection as boosting immunization is a scheme for preparing the tumor monoclonal antibody, and the scheme not only wastes time, but also has unsatisfactory immunization effect; therefore, the practical application of the immune method of directly injecting antigen into spleen reported in recent years abroad is continuously improved, and the intrasplenic injection method is taken as the conventional immune method in the present room to prepare the tumor monoclonal antibody; multiple practices prove that the method is superior to the tail vein injection method, the operation is simple and easy to implement, and the operation can be completed within 5 minutes.

The intrasplenic injection method mainly comprises the steps of , anaesthetizing a mouse model by using an ether inhalation method, fixing the mouse model on a fixing plate in a prone position, sequentially cutting open skin and peritoneum by taking the middle part of the left trunk of the mouse model as a tangential point, exposing the spleen, penetrating a needle tip into a sample body of the mouse model from a spleen tail to a spleen head, withdrawing the needle and injecting at an inappropriately high speed, and pushing the spleen back to the abdominal cavity to close the abdominal cavity.

Disclosure of Invention

The utility model aims to provide mouse model splenic injection fixing devices with simple structure and simple operation.

In order to achieve the above purpose, the utility model can adopt the following technical proposal:

mouse model splenic injection fixing device, including the mouse model fixed station the preceding lateral wall middle part of mouse model fixed station has vertically seted up logical groove, with it forms mouse body support groove to lead to the recessed formation in groove position corresponding mouse model fixed station upper surface middle part, lies in to lead to and has seted up the forelimb fixed slot on the left mouse model fixed station of groove, lies in to lead to and has seted up hind limb fixed slot and mouse tail fixed slot on the mouse model fixed station on groove right side all span on forelimb fixed slot, hind limb fixed slot and the mouse tail fixed slot has the magic sticky tape, the end of magic sticky tape links firmly with the mouse model fixed station mutually, its other end with fix the bonding piece swing joint at mouse model fixed station upper surface, be provided with the elasticity in the medial surface middle part of magic sticky tape, be provided with the metal forming hose in the angle department of mouse model fixed station, the upper end of metal forming hose links through universal bulb hinge has, is provided with in the mouse model fixed station for the charging source of light.

And the triangle of the mouse model fixing table corresponding to the forelimb fixing groove, the hindlimb fixing groove and the rat tail fixing groove is provided with a bandage hook.

The mouse model fixing table is characterized by further comprising an upper opening containing box matched with the mouse model fixing table, and an upper cover of a screen plate is hinged to the upper opening of the containing box.

The opening side edge of the screen plate upper cover is hinged with a locking plate, and the outer side wall of the box body is provided with a locking lug which is in adaptive clamping connection with the through hole on the locking plate.

pairs of handle rings are symmetrically hinged on the opposite outer side walls of the box body.

The utility model has the advantages of simple structure, convenient use and low manufacturing cost. The lateral position of the mouse model is fixed on the mouse model fixing table through the device, so that both hands of an operator are liberated, single-person operation is realized, and the working efficiency is improved; in addition, the illuminating lamp connected with the mouse model fixing table through the metal shaping hose can rotate at any angle, directly aims at the mouse model body with the skin incised and irradiates, is particularly suitable for the condition that the peritoneum cannot be cut off due to adhesion of mouse model tissues after immunization, facilitates the clear observation of spleen positions of operators, and improves the operation accuracy.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Detailed Description

As shown in fig. 1, the fixing device for intrasplenic injection of a mouse model comprises a mouse model fixing table 1, wherein a through groove 2 is longitudinally formed in the middle of the front side wall of the mouse model fixing table 1, and the through groove 2 is matched with the space between the front limb and the rear limb of the mouse model; with leading to the concave formation mouse body in 1 upper surface middle part of groove 2 corresponding mouse model fixed station in position and holding in the palm groove 3, be located and seted up forelimb fixed slot 4 on leading to groove 2 left mouse model fixed station 1, be located and seted up hind limb fixed slot 5 and mouse tail fixed slot 6 on leading to groove 2 right side mouse model fixed station 1, mouse body holds in the palm groove 3, forelimb fixed slot 4, the size of hind limb fixed slot 5 and mouse tail fixed slot 6 respectively with mouse model's health, forelimb, hind limb and tail phase-match.

The front limb fixing groove 4, the rear limb fixing groove 5 and the mouse tail fixing groove 6 are all provided with magic adhesive tapes 7 in a crossing mode, ends of the magic adhesive tapes 7 are fixedly connected with the mouse model fixing table 1, the other ends of the magic adhesive tapes are movably adhered to an adhesive block 8 fixed on the upper surface of the mouse model fixing table 1, and front limbs, rear limbs and tail bars placed in the front limb fixing groove 4, the rear limb fixing groove 5 and the mouse tail fixing groove 6 are fixed and clamped through the magic adhesive tapes 7.

angle departments at mouse model fixed station 1 are provided with metal forming hose 10, metal forming hose 10's upper end has light 12 through universal bulb 11 hinge, be provided with the charging source 13 for light 12 power supply in mouse model fixed station 1, metal forming hose 10 can buckle wantonly, cooperation universal bulb 11 makes light 12 can optional rotational position, direct alignment cuts the mouse model internal irradiation after the skin, especially be applicable to the condition that the peritoneum can't be cut open to the adhesion in mouse model tissue after the immunization.

In order to facilitate storage, the portable mouse model storage box further comprises an upper opening containing box 15 matched with the mouse model fixing table 1, the depth of an inner cavity of the containing box 15 is larger than the height of the mouse model fixing table 1, the mouse model fixing table 1 can be just placed in the containing box 15 for storage, meanwhile, a screen plate upper cover 16 is hinged to the upper opening of the containing box 15, when the mouse model fixing table 1 fixed with the mouse model is placed in the containing box 15, the screen plate upper cover 16 can be buckled to prevent the mouse model from escaping in steps, in order to prevent the screen plate upper cover 16 from being opened in steps, a locking plate 17 is hinged to the opening side edge of the screen plate upper cover 16, a locking lug 19 matched and clamped with a through hole 18 in the locking plate 17 is arranged on the outer side wall of the containing box 15, the screen plate upper cover 16 is locked through the locking plate 17 and the locking lug 19, in addition, pairs of handle rings 20 are symmetrically hinged to the opposite outer side wall of the containing box 15, and the carrying of the containing box.

During the use, only need to place the mouse model lateral decubitus position after anaesthetizing on mouse model fixed station 1, its health is arranged in mouse body and is held in the groove 3, two forelimbs blocks are put in forelimb fixed slot 4, two hind limbs are put in hind limb fixed slot 5, the tail card is put in tail fixed slot 6, fix forelimb, hind limb and tail through magic sticky tape 7, two forelimbs, two hind limbs and tail are worn respectively to overlap in corresponding elasticity is tied up and is tied up in ring 9 this moment, reinforcing mouse model forelimb, the fixed of hind limb and tail, later alright carry out subsequent experimental operation, can move light 12 at any time and shine in the operation process, the operating personnel of being convenient for can clearly observe each position of mouse model when the operation, improve the operation precision.

Claims (5)

1. The injection fixing device for the mouse model in the spleen is characterized by comprising a mouse model fixing table (1), a through groove (2) is longitudinally formed in the middle of the front side wall of the mouse model fixing table (1), a mouse body supporting groove (3) is formed by recessing the middle of the upper surface of the mouse model fixing table (1) corresponding to the through groove (2), a forelimb fixing groove (4) is formed in the mouse model fixing table (1) on the left side of the through groove (2), a hindlimb fixing groove (5) and a mouse tail fixing groove (6) are formed in the mouse model fixing table (1) on the right side of the through groove (2), magic adhesive tapes (7) stretch across the forelimb fixing groove (4), the hindlimb fixing groove (5) and the mouse tail fixing groove (6), a end of each magic adhesive tape (7) is fixedly connected with the mouse model fixing table (1), the other end of each magic adhesive tape is movably adhered to an adhesive block (8) fixed on the upper surface of the mouse model fixing table (1), a magic adhesive tape (7) is arranged in the middle of the inner side face of the magic tape (7), an elastic fixing table (9) is provided with a power supply ring (10) of a lighting lamp (10), and a universal lamp (12) is arranged in the mouse model fixing table (1), and a mouse model fixing table (10) and a mouse fixing table (10) is provided with a universal lamp fixing table (.
2. 2. The mouse model intrasplenic injection fixation device of claim 1, wherein: and bandage hooks (14) are arranged at the triangle positions of the mouse model fixing table (1) corresponding to the forelimb fixing groove (4), the hindlimb fixing groove (5) and the rat tail fixing groove (6).
3. 3. The mouse model intrasplenic injection fixation device of claim 1 or 2, wherein: the mouse model fixing table is characterized by further comprising an upper opening containing box (15) matched with the mouse model fixing table (1), and an upper screen plate cover (16) is hinged to the upper opening of the containing box (15).
4. 4. The mouse model intrasplenic injection fixation device of claim 3, wherein: the opening side edge of the mesh plate upper cover (16) is hinged with a locking plate (17), and the outer side wall of the containing box (15) is provided with a locking lug (19) which is in adaptive clamping connection with a through hole (18) on the locking plate (17).
5. 5. The injection fixing device in the spleen of a mouse model as claimed in claim 3, wherein pairs of handle rings (20) are symmetrically hinged on the opposite outer side walls of the containing box (15).

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