CN209989414U - Quick extractor of plant fungi DNA - Google Patents
Quick extractor of plant fungi DNA Download PDFInfo
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- CN209989414U CN209989414U CN201920165209.1U CN201920165209U CN209989414U CN 209989414 U CN209989414 U CN 209989414U CN 201920165209 U CN201920165209 U CN 201920165209U CN 209989414 U CN209989414 U CN 209989414U
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Abstract
The utility model relates to a plant fungi DNA's quick extractor. Which comprises a motor (1), a cracking box (16) and an adsorption box (19); the motor (1) is arranged on the machine cover (14), the machine cover (14) is buckled at the upper end of the cracking box (16), the machine cover (14) is provided with the sample adding cover (2), the sample adding cover (2) is provided with the filter cotton which is run through, and the cracking box (16) is filled with fungus lysate; the motor (1) is provided with a rotating rod (3) and a blade (4); a cracker (7) is arranged at the bottom of the cracking box (16); a filter block (5) is embedded in the center of the cracker (7), and the filter block (5) is supported on a screen (8); a liquid suction pump (9) is arranged below the screen (8); the lower end of the liquid suction pump (9) is provided with an upper clamping table (12), the bottom of the adsorption box (19) is provided with a lower clamping table (20) and a lifting nut (13), and a DNA adsorption film is clamped between the upper and lower clamping tables. The utility model discloses can draw DNA fast, accelerate detection speed.
Description
Technical Field
The utility model belongs to phytopathogen detection field, in particular to a plant fungi DNA rapid extractor.
Background
In the fields of agriculture, horticulture and the like, pathogenic bacteria on plant bodies are often required to be detected, the phenotypes of pathogenic fungi in different regions are different, DNA is the best way for distinguishing different pathogenic fungi, different fungi types are accurately and quickly identified, and the method is favorable for test and effective prevention and treatment; because many pathogenic fungi are parasitic in living bodies, the activity of the fungi is often influenced after the in vitro sampling of the plants, the pathogenic fungi are easy to spread to a certain extent, and some plant tissue samples are large; therefore, a convenient and labor-saving method is needed to facilitate the detection of plant pathogenic fungi.
SUMMERY OF THE UTILITY MODEL
To the inconvenient technical problem of present sample, this application provides a quick extractor of plant fungi DNA, and it only needs to carry out the fungi sample to plant pathological change department, directly carries out the DNA and draws, has reduced the inconvenience of sample.
The application relates to a quick extractor for plant fungi DNA, which is characterized by comprising a motor 1, a cracking box 16 and an adsorption box 19.
The motor 1 is arranged on a cover 14, the cover 14 is buckled at the upper end of the cracking box 16, a sample adding cover 2 is arranged on the cover 14, sample adding can be carried out when the sample adding cover 2 is opened, the sample adding cover 2 is provided with through filter cotton and can filter impurities in the outside air, and the outside air can enter the cracking box 16 through the filter cotton; the cracking box 16 is filled with fungus cracking liquid.
A rotating rod 3 is arranged at the lower part of the motor 1, and a blade 4 is arranged at the other end of the rotating rod 3; the bottom of the cracking box 16 is designed into an inclined conical surface 6 in the middle of the four circumferential directions, and the bottom of the cracking box 16 is provided with an annular cracker 7.
A filter block 5 is embedded in the center of the cracker 7; the bottom of the cracking box 16 is provided with a screen 8, and the filter block 5 is supported on the screen 8.
A liquid suction pump 9 is arranged below the screen 8, the liquid suction pump 9 is supported by a support piece 10, an upper clamping table 12 is arranged at the lower end of the liquid suction pump 9, and a tank opening 11 is arranged on the side wall of the lower part of the adsorption tank 19; the bottom of the adsorption box 19 is provided with a lower clamping table 20 and a lifting nut 13, the lower clamping table 20 is connected with a liquid outlet pipe 21, the liquid outlet pipe 21 can move up and down by rotating the lifting nut 13, so that the lower clamping table 20 and the upper clamping table 12 are clamped tightly, and a DNA adsorption film is clamped between the lower clamping table 20 and the upper clamping table 12.
A battery 18 is arranged at one side of the adsorption box 19 and provides electric energy for the liquid suction pump 9, the cracker 7 and the motor 1, and the inserting end 15 at the upper part of the line pipe 17 is inserted into the machine cover 14.
The cracker 7 is an ultrasonic cracker.
The combining surface of the lower clamping table 20 and the upper clamping table 12 has elasticity.
The utility model discloses a characteristics and beneficial effect do: (1) the blades of the motor stir the filter block, so that the filter block is effectively prevented from being blocked; (2) the plant fungi are not required to be sampled, and the DNA is directly extracted, so that the test progress is accelerated; (3) the filter block can be replaced; the design of the motor blades is beneficial to the cleaning of the extractor; (4) DNA adsorption films can be directly arranged between the upper clamping table and the lower clamping table, and the arrangement and the sampling are both convenient; (5) the ultrasonic design further accelerates the cracking of plant fungi and accelerates the extraction of DNA.
Drawings
FIG. 1 is a schematic cross-sectional view of a plant fungal DNA rapid extractor;
in the figure, 1-motor, 2-sample adding cover, 3-rotating rod, 4-blade, 5-filter block, 6-conical surface, 7-cracker, 8-screen, 9-liquid sucking pump, 10-supporting piece, 11-box mouth, 12-upper clamping table, 13-lifting nut, 14-machine cover, 15-inserting end, 16-cracking box, 17-line pipe, 18-battery, 19-adsorption box, 20-lower clamping table and 21-liquid outlet pipe.
Detailed Description
The specific structure and usage of the present invention will be further explained with reference to fig. 1 and the following embodiments.
The application relates to a plant fungi DNA rapid extractor, which is characterized by comprising a motor 1, a cracking box 16 and an adsorption box 19.
The motor 1 is arranged on a cover 14, the cover 14 is buckled at the upper end of the cracking box 16, a sample adding cover 2 is arranged on the cover 14, sample adding can be carried out when the sample adding cover 2 is opened, the sample adding cover 2 is provided with through filter cotton and can filter impurities in the outside air, and the outside air can enter the cracking box 16 through the filter cotton; the cracking box 16 is filled with fungus cracking liquid.
A rotating rod 3 is arranged at the lower part of the motor 1, and a blade 4 is arranged at the other end of the rotating rod 3; the bottom of the cracking box 16 is designed into an inclined conical surface 6 in the middle of the four circumferential directions, and the bottom of the cracking box 16 is provided with an annular cracker 7.
A filter block 5 is embedded in the center of the cracker 7; the bottom of the cracking box 16 is provided with a screen 8, and the filter block 5 is supported on the screen 8.
A liquid suction pump 9 is arranged below the screen 8, the liquid suction pump 9 is supported by a support piece 10, an upper clamping table 12 is arranged at the lower end of the liquid suction pump 9, and a tank opening 11 is arranged on the side wall of the lower part of the adsorption tank 19; the bottom of the adsorption box 19 is provided with a lower clamping table 20 and a lifting nut 13, the lower clamping table 20 is connected with a liquid outlet pipe 21, the liquid outlet pipe 21 can move up and down by rotating the lifting nut 13, so that the lower clamping table 20 and the upper clamping table 12 are clamped tightly, and a DNA adsorption film is clamped between the lower clamping table 20 and the upper clamping table 12.
A battery 18 is arranged on one side of the adsorption box 19 and provides electric energy for the liquid suction pump 9, the cracker 7 and the motor 1; the upper insertion end 15 of the line pipe 17 is inserted into the cover 14.
The cracker 7 is an ultrasonic cracker.
The combining surface of the lower clamping table 20 and the upper clamping table 12 has elasticity.
When in useThe DNA adsorption film is directly placed between an upper clamping table 12 and a lower clamping table 20 through a box opening 11, and a lifting nut 13 is screwed to clamp the DNA adsorption film; opening the sample adding cover 2, adding the lysate, and sampling pathogenic fungi and adding the pathogenic fungi into the lysate; starting the motor 1 and the cracker 7 to crack the plant fungi, and starting the liquid suction pump after the plant fungi are fully cracked; the lysate passes through a filter block 5 and a DNA adsorption membrane; and pass throughThe liquid outlet pipe 21 flows out, and waste liquid is filled into a waste liquid bottle; after no lysate exists in the extractor, screwing the lifting nut 13, taking out the crude DNA adsorption film and putting the DNA adsorption film into a storage tube for storage; screwing the lifting nut 13 again to clamp the upper clamping table 12 and the lower clamping table 20; opening the cover 14, taking out the filter block 5, adding cleaning liquid, then fastening the cover 14, starting the motor 1 to clean the cracking box 16, then starting the adsorption pump 9 to clean the pipeline, and repeating the steps for 3-4 times to clean the extractor; and a new filter block 5 is added for next sampling.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (3)
1. A plant fungus DNA rapid extractor is characterized in that the extractor comprises a motor (1), a cracking box (16) and an adsorption box (19);
the device comprises a motor (1), a cover (14), a sample adding cover (2) and a sample adding cover (2), wherein the motor (1) is arranged on the cover (14), the cover (14) is buckled at the upper end of a cracking box (16), the sample adding cover (2) is arranged on the cover (14), the sample adding cover can be used for adding samples when being opened, the sample adding cover (2) is provided with through filter cotton, impurities in outside air can be filtered, and the outside air can enter the cracking box (16) through the filter cotton; the cracking box (16) is filled with fungus cracking liquid;
a rotating rod (3) is arranged at the lower part of the motor (1), and a blade (4) is arranged at the other end of the rotating rod (3); the bottom of the cracking box (16) is designed into an inclined conical surface (6) in the middle of the four circumferential directions, and the bottom of the cracking box (16) is provided with an annular cracker (7);
a filter block (5) is embedded in the center of the cracker (7); the bottom of the cracking box (16) is provided with a screen (8), and the filter block (5) is supported on the screen (8);
a liquid suction pump (9) is arranged below the screen (8), the liquid suction pump (9) is supported by a support piece (10), an upper clamping table (12) is arranged at the lower end of the liquid suction pump (9), and a tank opening (11) is formed in the side wall of the lower part of the adsorption tank (19); the bottom of the adsorption box (19) is provided with a lower clamping table (20) and a lifting nut (13), the lower clamping table (20) is connected with a liquid outlet pipe (21), the liquid outlet pipe (21) can move up and down by rotating the lifting nut (13), so that the lower clamping table (20) and the upper clamping table (12) are clamped, and a DNA adsorption film is clamped between the lower clamping table (20) and the upper clamping table (12);
a battery (18) is arranged on one side of the adsorption box (19) and provides electric energy for the liquid suction pump (9), the cracker (7) and the motor (1); the insertion end (15) at the upper part of the circuit pipe (17) is inserted into the cover (14).
2. The plant fungi DNA rapid extractor according to claim 1, characterized in that the cracker (7) is an ultrasonic cracker.
3. The plant fungi DNA rapid extractor as claimed in claim 1, characterized in that the combination surface of the lower clamping table (20) and the upper clamping table (12) has elasticity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920165209.1U CN209989414U (en) | 2019-01-30 | 2019-01-30 | Quick extractor of plant fungi DNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920165209.1U CN209989414U (en) | 2019-01-30 | 2019-01-30 | Quick extractor of plant fungi DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209989414U true CN209989414U (en) | 2020-01-24 |
Family
ID=69288178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920165209.1U Active CN209989414U (en) | 2019-01-30 | 2019-01-30 | Quick extractor of plant fungi DNA |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209989414U (en) |
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2019
- 2019-01-30 CN CN201920165209.1U patent/CN209989414U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 510000 b1401, No. 66, Huacheng Avenue, Zhujiang New Town, Tianhe District, Guangzhou, Guangdong Patentee after: Guangzhou Customs Technology Center Address before: 510000 b1401, No. 66, Huacheng Avenue, Zhujiang New Town, Tianhe District, Guangzhou, Guangdong Patentee before: Inspection & Quarantine Technology Center of Guangdong Entry-Exit Inspection & Quarantine Bureau |
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| CP01 | Change in the name or title of a patent holder | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220104 Address after: 510663 Guangzhou International Business Incubator f702, No. 3, lanyue Road, Science City, Huangpu District, Guangzhou City, Guangdong Province Patentee after: Guangdong Huawei Testing Co.,Ltd. Address before: 510000 b1401, No. 66, Huacheng Avenue, Zhujiang New Town, Tianhe District, Guangzhou, Guangdong Patentee before: Guangzhou Customs Technology Center |
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| TR01 | Transfer of patent right |