CN209985430U - Packaging integrated multiple analysis paper microfluidic chip - Google Patents
Packaging integrated multiple analysis paper microfluidic chip Download PDFInfo
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- CN209985430U CN209985430U CN201920544955.1U CN201920544955U CN209985430U CN 209985430 U CN209985430 U CN 209985430U CN 201920544955 U CN201920544955 U CN 201920544955U CN 209985430 U CN209985430 U CN 209985430U
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- 238000004806 packaging method and process Methods 0.000 title claims abstract description 25
- 238000004458 analytical method Methods 0.000 title claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 230000001681 protective effect Effects 0.000 claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 12
- 239000007924 injection Substances 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 10
- 230000002209 hydrophobic effect Effects 0.000 claims description 7
- 230000010354 integration Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 21
- 239000012488 sample solution Substances 0.000 abstract description 12
- 238000000034 method Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000010146 3D printing Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000000016 photochemical curing Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 238000003698 laser cutting Methods 0.000 description 1
- 238000010147 laser engraving Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004038 photonic crystal Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
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Abstract
The utility model provides a packaging integrated multiple analysis paper microfluidic chip, which comprises a packaging shell and a paper microfluidic chip packaged in the packaging shell; the paper micro-fluidic chip comprises a sample injection area of a capillary micro-flow graphic structure positioned in the center of the paper micro-fluidic chip, reaction areas of the capillary micro-flow structure positioned at four outer end points of the paper micro-fluidic chip, and a flow channel of the capillary micro-flow structure for communicating the reaction areas with the sample injection area; the packaging shell comprises a base, a positioning cover plate and a protective cover; the base is provided with a cross-shaped base groove; the paper microfluidic chip is laid at the bottom of the groove of the base; and a cross-shaped protruding positioning structure which is embedded into the three-dimensional flow channel of the base groove and tightly presses the paper microfluidic chip is arranged on the positioning cover plate. The cross-shaped paper micro-fluidic chip can simultaneously carry out multiple analyses on one sample solution, and has high efficiency; the packaging structure is simple and firm, and the use is convenient.
Description
Technical Field
The utility model relates to a micro-fluidic chip especially relates to an encapsulation integrated multiple analysis paper micro-fluidic chip.
Background
The micro-fluidic chip is also called a lab-on-a-chip, which is an important branch of micro total analysis system, and integrates functional devices such as micro-pipeline, micro-pump, micro-valve, micro-reservoir, micro-motor, micro-detection element, window and connector on the chip material (substrate) like integrated circuit by micro-processing technology.
Basic operation units such as sample preparation, biological and chemical reaction, separation, detection and the like can be integrated in the paper microfluidic chip system, and a network is formed by microchannels, so that controllable fluid penetrates through the whole system, and various functions of a conventional laboratory are realized. The existing paper microfluidic chip has single structure function, complex packaging structure and inconvenient use.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a packaging integrated multiple analysis paper microfluidic chip which can simultaneously carry out multiple analyses on a sample solution and has high efficiency; the packaging structure is simple and firm, and the use is convenient.
The technical scheme is as follows: the utility model provides a packaging integrated multiple analysis paper microfluidic chip, which comprises a packaging shell and a cross paper microfluidic chip packaged in the packaging shell;
the paper micro-fluidic chip comprises a sample injection area of a capillary micro-flow pattern structure positioned in the center of the cross-shaped paper micro-fluidic chip, reaction areas of the capillary micro-flow structure positioned at four outer end points of the cross-shaped paper micro-fluidic chip, and cross-shaped flow channels of the capillary micro-flow structure for communicating the reaction areas and the sample injection areas;
the packaging shell comprises a base, a positioning cover plate and a protective cover; the base is provided with a cross-shaped base groove; the base groove comprises a base groove liquid inlet cavity positioned at the center of the cross-shaped base groove, a base groove reaction cavity positioned at four outer end points of the cross-shaped base groove, and a cross-shaped base groove three-dimensional flow channel for communicating the base groove reaction cavity with the base groove liquid inlet cavity;
the paper microfluidic chip has the same size as the groove of the base, and is laid at the bottom of the groove of the base; a hydrophobic film material layer is also arranged between the paper microfluidic chip and the groove of the base;
the positioning cover plate is provided with a cross-shaped protruding positioning structure which is embedded into the three-dimensional flow channel of the base groove and tightly presses the paper microfluidic chip; the positioning cover plate is also provided with four observation holes and a sample inlet hole; the four observation holes are respectively positioned right above the four base groove reaction cavities; the sample inlet hole is positioned right above the liquid inlet cavity of the groove of the base;
the protective cover is connected with the base and is packaged outside the paper microfluidic chip.
Further, the base is connected with the protective cover through a hinge structure.
Furthermore, the hinge structure comprises a rotating hole arranged on the base and a rotating shaft arranged on the protective cover.
Furthermore, a lock catch structure is arranged between the base and the protective cover.
Furthermore, the locking structure comprises a locking groove arranged on the base and a locking block which is arranged on the protective cover and can be embedded into the locking groove.
Has the advantages that: the cross-shaped paper micro-fluidic chip can simultaneously carry out multiple analyses on one sample solution, and has high efficiency; the packaging structure is simple and firm, and the use is convenient.
Drawings
Fig. 1 is a schematic exploded view of the present invention;
FIG. 2 is a schematic view of the assembly structure of the present invention;
fig. 3 is a schematic structural diagram of the paper microfluidic chip of the present invention;
FIG. 4 is a first schematic view of the base structure of the present invention;
FIG. 5 is a schematic view of a base structure of the present invention;
fig. 6 is a schematic structural view of the positioning cover plate of the present invention;
fig. 7 is a schematic structural diagram of the protective cover of the present invention.
Detailed Description
As shown in fig. 1 and fig. 2, the utility model provides a package integrated multiple analysis paper microfluidic chip, including the package shell and the cross paper microfluidic chip 1 of encapsulation in the package shell.
As shown in fig. 3, the paper microfluidic chip structure 1 is prepared by cutting methods such as laser cutting and mechanical cutting, and the material of the paper microfluidic chip structure 1 may be a sheet material with a fiber microstructure, such as filter paper, NC film, silk, cotton cloth, and the like, which can perform capillary flow. The paper micro-fluidic chip 1 comprises a sample injection area 11 of a capillary micro-flow structure positioned at the center of the cross paper micro-fluidic chip 1, reaction areas 12 of the capillary micro-flow structure positioned at four outer end points of the cross paper micro-fluidic chip 1, and cross flow channels 13 of the capillary micro-flow structure communicating the reaction areas 12 with the sample injection area 11. By adding test samples of different compositions to the four reaction regions 12, it is possible to simultaneously perform a plurality of analyses on one sample solution fed from the sample introduction region 11.
The packaging shell comprises a base 2, a positioning cover plate 3 and a protective cover 4. As shown in fig. 4 and 5, the base 2 is provided with a cross-shaped base groove; the base groove comprises a base groove liquid inlet cavity 21 positioned at the center of the cross-shaped base groove, a base groove reaction cavity 22 positioned at four outer end points of the cross-shaped base groove, and a cross-shaped base groove three-dimensional flow channel 23 for communicating the base groove reaction cavity 22 with the base groove liquid inlet cavity 21.
The paper microfluidic chip 1 and the base groove have the same size, and the paper microfluidic chip 1 is laid at the bottom of the base groove; and a hydrophobic film material layer 24 is also arranged between the paper microfluidic chip 1 and the groove of the base.
As shown in fig. 6, a cross-shaped protrusion positioning structure 33 which is embedded in the base groove three-dimensional flow channel 23 and compresses the paper microfluidic chip 1 is arranged on the positioning cover plate 3; the positioning cover plate 3 is also provided with four observation holes 32 and a sample inlet hole 31; the four observation holes 32 are respectively positioned right above the four base groove reaction cavities 22; the sampling hole 31 is positioned right above the liquid inlet cavity 21 of the base groove.
As shown in fig. 7, the protective cover 4 is connected to the base 2 and is encapsulated outside the paper microfluidic chip 1.
The base 2 is connected with the protective cover 4 through a hinge structure. The hinge structure includes a rotation hole 26 provided on the base 2 and a rotation shaft 42 provided on the protection cover 4.
A lock catch structure is also arranged between the base 2 and the protective cover 4. The locking structure comprises a locking groove 25 arranged on the base 2 and a locking block 41 arranged on the protective cover 4 and capable of being embedded into the locking groove 25.
The preparation method of the packaging integrated multiple analysis paper microfluidic chip comprises the following steps:
first step, preparing paper micro-fluidic chip 1
The paper microfluidic chip 1 is characterized in that a microfluidic graphic structure is firstly designed according to requirements, the graphic structure comprises a sample injection area, a flow channel, a reaction area and the like, the graphic structure is a complete and interconnected structure, and then the graphic structure is manufactured by adopting a laser engraving technology on sheet raw materials with fiber structures, such as filter paper, an NC (numerical control) film, silk, cotton cloth and the like.
Second step, preparing the base 2
The base 2 is used for bearing the paper microfluidic chip 1, a three-dimensional model is designed according to the shape of the paper microfluidic chip 1, the model comprises a base groove, the shape of the base groove is completely consistent with that of the paper microfluidic chip 1 in the first step, so that the paper microfluidic chip and the base groove can be assembled conveniently, and the assembly structure is used for packaging and matching; the base 2 is then fabricated in a fused deposition or photocuring three-dimensional printing process.
Thirdly, preparing the positioning cover plate 3
The positioning cover plate 3 is used for attaching and fixing the paper microfluidic chip 1 in the first step and the base groove in the second step; the positioning cover plate 3 comprises a convex positioning structure 33, a viewing hole 32 and a sample inlet hole 31; the shape of the protruding positioning structure 33 is completely the same as the base groove flow channel described in the second step, but its size is properly reduced to facilitate the assembly of the two; the sample inlet hole 31 is a circular hole, and the size of the sample inlet hole is slightly larger than the sample inlet area 11 of the paper microfluidic chip 1 in the step one; the sample solution is dripped into the sample injection area 11 from the sample injection hole 31; the observation hole 32 is an arc observation hole, and the size of the observation hole is slightly larger than the reaction area 12 of the paper microfluidic chip 1 so as to observe the reaction condition of the sample solution and the test solution; the positioning cover plate 3 is prepared by adopting a fused deposition or photocuring three-dimensional printing method.
The fourth step is to prepare a protective cover 4
The protective cover 4 is used for packaging and protecting the paper microfluidic chip 1, the base 2 and the positioning cover plate 3; the protective cover is prepared by adopting a fused deposition or photocuring three-dimensional printing method.
Fifth, packaging
5.1 covering a hydrophobic film material layer 24 at the bottom of the base groove;
5.2 covering the paper microfluidic chip 1 on the hydrophobic film material layer 24;
5.3 covering the paper microfluidic chip 1 with the positioning cover plate 3 and pressing tightly;
5.4 the protective cover 4 is encapsulated.
The preparation and test method of the packaging integrated multiple analysis paper microfluidic chip includes the following steps:
example 1
The packaging integrated detection preparation of the multiple analysis paper microfluidic chip comprises the following specific steps:
And 5, covering a hydrophobic film material layer 24 at the groove of the base, covering the paper microfluidic chip 1 on the hydrophobic film material layer 24, aligning the convex positioning structure 33 of the positioning cover plate 3 to the three-dimensional flow channel 23 of the groove of the base, and tightly pressing, and assembling the rotating hole 26 on the base 2 and the rotating shaft 42 arranged on the protective cover 4.
And 6, adding a sample solution into the sample inlet hole 31, adding glucose oxidase into the observation hole 32, wherein the sample solution in the liquid inlet cavity 21 flows to the reaction cavity 22 due to the capillary phenomenon of the paper microfluidic chip 1 and reacts with the glucose oxidase in the reaction cavity 22, so as to detect whether the sample solution contains glucose.
Example 2
And 6, adding a sample solution into the sample inlet hole 31, adding photonic crystal microspheres into the observation hole 32, and allowing the sample solution in the liquid inlet cavity 21 to flow into the reaction cavity 22 due to the capillary phenomenon of the paper microfluidic chip 1 so as to detect the aflatoxin B1 in the sample solution.
Claims (5)
1. The utility model provides a package integration's multiple analysis paper micro-fluidic chip which characterized in that: comprises a packaging shell and a cross-shaped paper microfluidic chip (1) packaged in the packaging shell;
the paper micro-fluidic chip (1) comprises a sample injection area (11) of a capillary micro-flow pattern structure positioned in the center of the cross paper micro-fluidic chip (1), reaction areas (12) of the capillary micro-flow structure positioned at four outer end points of the cross paper micro-fluidic chip (1), and cross flow channels (13) of the capillary micro-flow structure for communicating the reaction areas (12) with the sample injection area (11);
the packaging shell comprises a base (2), a positioning cover plate (3) and a protective cover (4); the base (2) is provided with a cross-shaped base groove; the base groove comprises a base groove liquid inlet cavity (21) positioned at the center of the cross-shaped base groove, a base groove reaction cavity (22) positioned at four outer end points of the cross-shaped base groove, and a cross-shaped base groove three-dimensional flow channel (23) for communicating the base groove reaction cavity (22) with the base groove liquid inlet cavity (21);
the paper microfluidic chip (1) and the base groove have the same size, and the paper microfluidic chip (1) is laid at the bottom of the base groove; a hydrophobic film material layer (24) is also arranged between the paper microfluidic chip (1) and the groove of the base;
the positioning cover plate (3) is provided with a cross-shaped convex positioning structure (33) which is embedded into the base groove three-dimensional flow passage (23) and tightly presses the paper microfluidic chip (1); the positioning cover plate (3) is also provided with four observation holes (32) and a sample inlet hole (31); the four observation holes (32) are respectively positioned right above the four base groove reaction cavities (22); the sample inlet hole (31) is positioned right above the liquid inlet cavity (21) of the groove of the base;
the protective cover (4) is connected with the base (2) and is packaged outside the paper microfluidic chip (1).
2. The packaged integrated, multi-analytical paper microfluidic chip according to claim 1, wherein: the base (2) is connected with the protective cover (4) through a hinge structure.
3. The packaged integrated, multi-analytical paper microfluidic chip according to claim 2, wherein: the hinge structure comprises a rotating hole (26) arranged on the base (2) and a rotating shaft (42) arranged on the protective cover (4).
4. The packaged integrated, multi-analytical paper microfluidic chip according to claim 1, wherein: and a lock catch structure is also arranged between the base (2) and the protective cover (4).
5. The packaged integrated multi-analysis paper microfluidic chip of claim 4, wherein: the lock catch structure comprises a lock groove (25) arranged on the base (2) and a lock block (41) which is arranged on the protective cover (4) and can be embedded into the lock groove (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920544955.1U CN209985430U (en) | 2019-04-22 | 2019-04-22 | Packaging integrated multiple analysis paper microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920544955.1U CN209985430U (en) | 2019-04-22 | 2019-04-22 | Packaging integrated multiple analysis paper microfluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209985430U true CN209985430U (en) | 2020-01-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920544955.1U Active CN209985430U (en) | 2019-04-22 | 2019-04-22 | Packaging integrated multiple analysis paper microfluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209985430U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111551544A (en) * | 2020-05-06 | 2020-08-18 | 东南大学 | Bivalent copper ion rapid detection device and detection method based on paper-based micro-fluidic chip |
-
2019
- 2019-04-22 CN CN201920544955.1U patent/CN209985430U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111551544A (en) * | 2020-05-06 | 2020-08-18 | 东南大学 | Bivalent copper ion rapid detection device and detection method based on paper-based micro-fluidic chip |
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