CN209974787U - A variable gravity cell experimental device based on three-dimensional rotation - Google Patents

Abstract

The utility model discloses a variable gravity cell experimental device based on three-dimensional rotation, which comprises a three-dimensional rotating unit, a cell constant direction stress unit fixed on the three-dimensional rotating unit and a monitoring control unit connected with the three-dimensional rotating unit; the cell culture bottle is fixed on the cell constant direction stress unit, can rotate around a spherical surface along with the three-dimensional rotating unit to generate centripetal acceleration in each direction, the corresponding centrifugal force and gravity can form resultant force with constantly changing magnitude and direction, the cell constant direction stress unit can enable the cell culture bottle to constantly and synchronously change with the direction of the resultant force, the monitoring and controlling unit can monitor the stress condition of the cells and change the stress magnitude of the cells by regulating and controlling the rotating speed of the three-dimensional rotating unit, and finally the cells can feel equivalent gravity stimulation with variable magnitude. The utility model discloses can be used to the influence of different mechanical environment such as step-down gravity, time-varying hypergravity and coriolis force to the cell function when studying.

Description

A variable gravity cell experimental device based on three-dimensional rotation

technical field

The utility model belongs to the field of biomechanical experimental equipment, in particular to a variable gravity cell experimental device based on three-dimensional rotation.

Background technique

In recent years, my country's manned spaceflight industry has developed rapidly, gradually moving towards the stage of medium and long-term space flight. The microgravity environment generated during spaceflight will cause a series of changes in the human cardiovascular system, skeletal muscle system, etc., which will seriously endanger the health of astronauts. Therefore, it is necessary to study the characteristics and laws of human physiological changes under microgravity, especially to study the mechanism at the cellular and molecular levels, and then propose targeted protective measures. In view of the limitations of spaceflight opportunities and costs, aerospace medicine researchers have carried out a large number of ground simulated microgravity experiments and discovered a series of molecular targets and signaling pathways involved in microgravity affecting human physiological functions, but microgravity or weightlessness has not been fully revealed The initial mechanism and development mechanism of the environment leading to human physiological dysfunction.

At present, gyrators are widely used at home and abroad to simulate the microgravity effect at the cellular level on the ground. On the gyrator, the biological sample remains in the gravitational field, subject to a constant gravitational vector. However, due to the rotation of the gyroscope, the moving direction of the biological samples it carries changes constantly, and it is still too late to respond to the gravity in a certain direction, thus simulating the biological effects of microgravity of cells in spaceflight. The gyroscope provides a cost-effective way to simulate the biological effects and occurrence mechanisms at the cellular level under microgravity conditions on the ground. However, there is no constant microgravity in the real aerospace environment, but is formed by a variety of mechanical environments that change from time to time. Microgravity and low gravity force fields, while the gyrator can only simulate constant microgravity experimental conditions, and cannot achieve a low gravity experimental condition or a gradient change experimental condition setting from microgravity to normal gravity to hypergravity, which may miss many Discovery of important regulatory factors or signaling molecules.

SUMMARY OF THE INVENTION

In view of the deficiencies of the prior art, the purpose of the present invention is to provide a variable gravity cell experimental device based on three-dimensional rotation, which overcomes the experimental conditions that the effect of time-varying gravity on cell function cannot be realized in the current research on simulated microgravity biological effects. Defects.

In order to solve the above-mentioned technical problems, the utility model adopts the following technical solutions to be realized:

A variable gravity cell experiment device based on three-dimensional rotation, comprising a three-dimensional rotation unit, a cell constant force unit fixed on the three-dimensional rotation unit, and a monitoring and control unit connected to the three-dimensional rotation unit; Radial swivel ring and pivoting swivel ring;

The outer sides of the two ends of the horizontal diameter of the radial rotating ring are arranged on the bracket through the shaft sleeve and the wheel axle, and the bracket on one side of the wheel shaft is provided with a first motor connected to the wheel shaft, which is used to drive the wheel shaft to drive the wheel shaft to rotate around its diameter 360-degree rotation of the horizontal diameter;

The rotating ring around the axis is connected to the inner side of the rotating ring around the diameter through the anchor pulley, and can rotate in the circumferential direction relative to the rotating ring around the diameter; The shaft, the spur gear shaft is connected with a second motor, which is used to drive the spur gear shaft to drive the circumferential rotation of the rotating ring around the shaft; the second motor is fixed on the rotating ring around the diameter;

Both the first motor and the second motor are connected to the monitoring control unit.

The utility model also includes the following technical features:

Optionally, the cell constant-direction force-bearing unit includes a semi-annular support, an outer ring and an inner ring that are arranged concentrically from outside to inside, and also includes a support rod disposed at the lower end of the semi-annular support, disposed on the inner ring and used To place the storage plate of the cell culture flask, the pyramid bracket set on the object plate, and the weight set at the tip of the pyramid bracket;

The outer sides of the two ends in the diameter direction of the outer ring are connected with the inner side of the semi-annular bracket through the shaft sleeve, so that the outer ring can realize the rotation with its own diameter as the rotation center axis of the outer ring, and the outer sides of the two ends in the diameter direction of the inner ring are connected with the outer ring through the shaft sleeve and the outer ring. Both ends of the inner side are connected, so that the inner ring can rotate with its own diameter as the rotation center axis of the inner ring; the rotation center axis of the outer ring and the inner ring rotation center axis are perpendicular to each other;

The left and right ends of the inner side of the inner ring are connected with the two ends of the storage board through bushings, so that the storage board can rotate with its own diameter as the rotation center axis of the storage board; the rotation center axis of the storage board and the rotation center axis of the inner ring are perpendicular to each other;

The cone support is fixed on the back of the storage board, and the vertical distance from the top of the weight to the bottom surface of the cone support is less than the radius of the inner ring;

The lower end of the support rod is fixed on the inner side of the rotating ring around the axis, the upper end of the support rod is installed with the semi-ring support, the opening of the semi-ring support is upward, and the bottom of the semi-ring support is fixed on the upper end of the support rod.

Optionally, the support rod is a rotary telescopic structure, and the length can be adjusted.

Optionally, the monitoring control unit includes an acceleration sensor and a controller;

The acceleration sensor is arranged on the bottom surface of the cone support, which can keep synchronous rotation with the cell culture flask placed on the object plate, and monitors the acceleration received by the cell culture flask at all times, and the acceleration sensor is connected with the controller by radio.

The controller is connected to the first motor and the second motor, respectively.

Optionally, the tooth pattern on the outer surface of the rotating ring around the axis is helical teeth, and is in contact with the tooth pattern on the spur gear shaft; one end of the spur gear shaft is connected to the radial rotating ring through a bushing, and the other end is the second motor is connected;

The front and rear sides of the rotating ring around the axis are provided with annular chute;

The inner side of the radially rotating ring is provided with a guide ring groove.

Optionally, the anchor pulley comprises a U-shaped frame with a downward opening, a shaft vertically fixed at the middle position of the outer upper end of the U-shaped frame top plate, and two balls arranged on the inner walls of the two side plates of the U-shaped frame;

the shaft rod is fixed in the guide ring groove of the radial rotating ring;

The ball is limited between the annular chute of the rotating ring around the axis and the inner wall of the side plate of the U-shaped frame, and the rotating ring around the axis is limited between the two balls on the inner wall of the two side plates of the U-shaped frame, so as to make the rotation The shaft rotating ring slides between the two balls of the anchoring pulley to ensure that the rotating ring around the shaft can rotate in the radial direction simultaneously with the rotating ring around the radius.

Optionally, there are four anchoring pulleys, and the four anchoring pulleys are arranged in quarters of the rotating ring around the axis.

Optionally, there are a plurality of screw holes on the inner side of the rotating ring around the axis, so as to install a plurality of cell constant-direction force-bearing units on the inner side of the rotating ring around the axis.

Compared with the prior art, the utility model has the following technical effects:

(I) The utility model includes a three-dimensional rotation unit, a cell constant force unit, a monitoring and control unit, etc. The cell culture flask is fixed on the shelf of the cell constant force unit, and the cell constant force unit can follow the three-dimensional rotation unit. Rotating around the spherical surface generates centripetal acceleration in all directions, so that the cells are subjected to the centrifugal force in the corresponding direction away from the center of the sphere.

(II) The utility model can make the cell culture flask always change synchronously with the direction of the resultant force through the cell constant force bearing unit. Relative rotation can occur between the rings, so that the weight connected to the storage plate can freely reach any point on the spherical surface. The resultant force generated by centrifugal force and gravity acts on the weight, and the weight drives the storage plate and the cell culture flask on it to rotate. , so that the resultant force always acts vertically on the cell culture flask. The monitoring and control unit can monitor the force of the cells and change the direction and magnitude of the centrifugal force by regulating the respective rotational speeds of the two motors in the three-dimensional rotating unit, and finally make the cells feel the same direction and size. Adjustable Equivalent Gravity Stimulation.

(III) The present utility model controls the respective rotational speeds of the rotating ring around the diameter and the rotating shaft around the axis through the monitoring and control unit, and can control the magnitude and direction of the generated centrifugal force. For example, when the cell culture flask rotates with the cell constant force unit It is located in the upper hemisphere or the lower hemisphere, thereby producing the effect of time-varying low gravity or time-varying supergravity; the utility model can be used to study the effects of different mechanical environments such as time-varying low gravity, time-varying supergravity and Coriolis force on cells functional impact.

Description of drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention.

FIG. 2 is a schematic structural diagram of a three-dimensional rotating unit of the present invention.

FIG. 3 is a schematic structural diagram of a cell constant-direction force-bearing unit of the present invention.

FIG. 4 is a schematic structural diagram of the anchoring pulley of the present invention.

The meaning of each label in the figure is: 1-three-dimensional rotation unit, 2-cell constant force unit, 3-monitoring control unit;

11-Radial swivel ring, 12-Axial swivel ring, 13-Bracket, 14-First motor, 15-Anchor pulley, 151-U-shaped frame, 152-Shaft rod, 153-Side plate, 154-Ball ball, 16-Spur gear shaft, 17-Second motor;

21-Semi-ring bracket, 22-Outer ring, 23-Inner ring, 24-Support rod, 25-Organization board, 26-Conical bracket, 27-Heavy hammer;

31-accelerometer, 32-controller.

Detailed ways

Specific embodiments of the present utility model are given below. It should be noted that the present utility model is not limited to the following specific embodiments, and all equivalent transformations made on the basis of the technical solutions of the present application all fall into the protection scope of the present utility model.

Example 1:

Following the above technical solutions, as shown in FIGS. 1 to 4 , this embodiment provides a three-dimensional rotation-based variable-gravity cell experiment device, including a three-dimensional rotation unit 1 and a constant force of cells fixed on the three-dimensional rotation unit 1 The unit 2 and the monitoring and control unit 3 connected to the three-dimensional rotating unit 1; the three-dimensional rotating unit 1 includes a concentrically arranged radial-circling ring 11 and an axis-circling rotating ring 12; Following spherical motion, centripetal acceleration in all directions is generated, and the corresponding centrifugal force and gravity form a resultant force that changes constantly. The outer sides of the two ends of the horizontal diameter of the diameter-circling rotating ring 11 are arranged on the bracket 13 through the shaft sleeve and the wheel axle. Rotate 360 degrees around its horizontal diameter; the rotating ring 12 around the axis is connected to the inner side of the rotating ring 11 through the anchor pulley 15, and can rotate in the circumferential direction relative to the rotating ring 11; the outer side of the rotating ring 12 is provided with Tooth pattern, a spur gear shaft 16 is meshed with the outside of the rotating ring 12 around the shaft, and the spur gear shaft 16 is connected with a second motor 17 to drive the spur gear shaft 16 to drive the rotating ring 12 to rotate in the circumferential direction. The second motor 17 is fixed on The first motor 14 and the second motor 17 are connected to the monitoring control unit 3 to realize the control of the first motor 14 and the second motor 17 by the monitoring control unit 3. Specifically, in this embodiment , both the first motor 14 and the second motor 17 can be connected to the monitoring control unit 3 through conductive brushes. The cell constant force unit 2 of the present invention can rotate around the spherical surface with the three-dimensional rotation unit 1 to generate centripetal acceleration in all directions, and the corresponding centrifugal force and gravity can form a resultant force that changes constantly. The cell constant force unit 2 can make The cell culture flask always changes synchronously with the direction of the resultant force. The monitoring and control unit 3 can monitor the force on the cells and change the direction and magnitude of the centrifugal force by regulating the respective rotational speeds of the two motors in the three-dimensional rotation unit 1, so that the cells feel the direction unchanged. Equivalent gravity stimuli with adjustable size.

As shown in FIG. 1 and FIG. 3 , the cell constant force bearing unit 2 includes a semi-annular support 21 , an outer ring 22 and an inner ring 23 arranged concentrically from the outside to the inside, and also includes a support rod disposed at the lower end of the semi-annular support 21 24. The storage plate 25 arranged on the inner ring 23 and used to place the cell culture flask, the pyramid bracket 26 arranged on the storage plate 25 and the weight 27 arranged at the tip of the pyramid bracket 26; the diameter direction of the outer ring 22 The outer sides of the two ends are connected with the inner side of the semi-ring support 21 through the shaft sleeve, so that the outer ring 22 can realize the rotation with its own diameter as the rotation center axis of the outer ring, and the outer sides of the inner ring 23 in the diameter direction are connected to the inner side of the outer ring 22 through the shaft sleeve. The ends are connected so that the inner ring 23 can rotate with its own diameter as the central axis of rotation of the inner ring; the central axis of rotation of the outer ring and the central axis of rotation of the inner ring are perpendicular to each other; The two ends are connected, so that the storage board 25 can rotate with its own diameter as the rotation center axis of the storage board; the rotation center axis of the storage board and the inner ring rotation center are perpendicular to each other; the back of the storage board 25 is fixed with a cone bracket 26 and a weight 27 The vertical distance from the top to the bottom surface of the cone support 26 is smaller than the radius of the inner ring 23 . The cell constant force unit 2 is fixed on the inner side of the rotating ring 12 through the support rod 24, and can perform spherical motion accordingly; under the combined effect of centrifugal force and gravity, the weight 27 passes through the outer ring 22, inner ring 23 and the storage The small three-dimensional rotating structure formed by the plate 25 can freely reach any position on the spherical surface, and always keep the same direction as the resultant force. cells within it.

More specifically, the lower end of the support rod 24 is fixed on the inner side of the pivot ring 12 , the upper end of the support rod 24 is mounted with a semi-annular bracket 21 , and the opening of the semi-annular bracket 21 is upward, and the bottom of the semi-annular bracket 21 is fixed on the upper end of the support rod 24 .

In this embodiment, the support rod 24 is a rotary telescopic structure, and the length can be adjusted, so that the cells can generate different rotation radii to meet the needs of adjusting the centrifugal force, which is suitable for different test requirements.

As shown in FIG. 1 and FIG. 3 , the monitoring and control unit 3 includes an acceleration sensor 31 and a controller 32; The acceleration received by the cell culture flask is constantly monitored, and the acceleration sensor 31 is connected to the controller 32 by radio.

The controller 32 is connected to the first motor 14 and the second motor 17 respectively, and can respectively control the two motors to rotate at different speeds.

The tooth pattern on the outer side of the rotating ring 12 is helical, and it is in contact with the tooth pattern on the spur gear shaft 16; Connected; the front and rear sides of the rotating ring 12 around the axis are provided with annular sliding grooves; the inner side of the rotating ring 11 around the diameter is provided with a guide ring groove.

The anchoring pulley 15 includes a U-shaped frame 151 with a downward opening, a shaft rod 152 vertically fixed at the middle position of the outer upper end of the U-shaped frame 151, and two balls 154 arranged on the inner walls of the two side plates 153 of the U-shaped frame 151; the shaft rod 152 is fixed in the guide ring groove of the rotating ring 11; the ball 154 is limited between the annular chute of the rotating ring 12 and the inner wall of the side plate 153 of the U-shaped frame 151, and the rotating ring 12 is limited in Between the two balls 154 on the inner walls of the two side plates 153 of the U-shaped frame 151, the rotating ring 12 around the axis slides between the two balls 154 of the anchoring pulley 15 to ensure that the rotating ring 12 can rotate around the axis The ring 11 rotates circumferentially while synchronously rotating around the radius.

In this embodiment, there are four anchoring pulleys 15 , and the four anchoring pulleys 15 are arranged at the quartered positions of the rotating ring 12 around the axis, so as to realize the concentric arrangement of the rotating ring 12 around the axis and the rotating ring 11 around the axis , and realize that the rotating ring 12 around the axis can rotate in the radial direction simultaneously with the rotating ring 11 around the radial direction.

There are a plurality of screw holes on the inner side of the rotating ring 12 around the axis for installing a plurality of cell constant force units 2 on the inner side of the rotating ring 12 around the axis.

The preferred embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the specific details of the above-mentioned embodiments. Within the scope of the technical concept of the present invention, many technical solutions of the present invention can be carried out. These simple modifications all belong to the protection scope of the present invention.

In addition, it should be noted that each specific technical feature described in the above-mentioned specific implementation manner may be combined in any suitable manner under the circumstance that there is no contradiction. In order to avoid unnecessary repetitions, various possible combinations are not described in the present invention.

In addition, the various embodiments of the present invention can also be arbitrarily combined, as long as they do not violate the idea of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims (8)

1. A variable gravity cell experimental device based on three-dimensional rotation is characterized by comprising a three-dimensional rotating unit (1), a cell constant direction stress unit (2) fixed on the three-dimensional rotating unit (1) and a monitoring control unit (3) connected with the three-dimensional rotating unit (1); the three-dimensional rotating unit (1) comprises a diameter-winding rotating ring (11) and a shaft-winding rotating ring (12) which are concentrically arranged;

the outer sides of two horizontal ends of the diameter-winding rotating ring (11) are arranged on a support (13) through a shaft sleeve and a wheel shaft, and a first motor (14) connected with the wheel shaft is arranged on the support (13) on one side of the wheel shaft;

the rotating ring (12) around the shaft is connected to the inner side of the rotating ring (11) around the diameter through an anchoring pulley (15) and can rotate circumferentially relative to the rotating ring (11) around the diameter; the outer side of the rotating shaft ring (12) is provided with insections, the outer side of the rotating shaft ring (12) is meshed with a straight gear shaft (16), the straight gear shaft (16) is connected with a second motor (17), and the second motor (17) is fixed on the rotating shaft (11);

the first motor (14) and the second motor (17) are connected with the monitoring control unit (3).

2. The variable gravity cell experiment device based on three-dimensional rotation as claimed in claim 1, wherein the cell constant direction stress unit (2) comprises a semi-annular support (21), an outer ring (22) and an inner ring (23) which are concentrically arranged from outside to inside, a support rod (24) arranged at the lower end of the semi-annular support (21), a placing plate (25) arranged on the inner ring (23) and used for placing a cell culture bottle, a cone support (26) arranged on the placing plate (25), and a heavy hammer (27) arranged at the tip of the cone support (26);

the outer sides of two ends of the outer ring (22) in the diameter direction are connected with the inner side of the semi-annular support (21) through shaft sleeves, so that the outer ring (22) can rotate by taking the diameter of the outer ring as an outer ring rotation central shaft, and the outer sides of two ends of the inner ring (23) in the diameter direction are connected with two ends of the inner side of the outer ring (22) through shaft sleeves, so that the inner ring (23) can rotate by taking the diameter of the inner ring as an inner ring rotation central shaft; the outer ring rotation central shaft is vertical to the inner ring rotation central shaft;

the left end and the right end of the inner side of the inner ring (23) are connected with the two ends of the object placing plate (25) through shaft sleeves, so that the object placing plate (25) can rotate by taking the diameter of the object placing plate as a rotating central shaft of the object placing plate; the rotating central shaft of the object placing plate is vertical to the rotating central shaft of the inner ring;

the cone support (26) is fixed on the back of the object placing plate (25), and the vertical distance from the top of the heavy hammer (27) to the bottom surface of the cone support (26) is smaller than the radius of the inner ring (23);

the lower end of the supporting rod (24) is fixed on the inner side of the rotating shaft ring (12), the upper end of the supporting rod (24) is provided with the semi-annular support (21), the opening of the semi-annular support (21) is upward, and the bottom of the semi-annular support (21) is fixed on the upper end of the supporting rod (24).

3. The variable gravity cell experiment device based on three-dimensional rotation as claimed in claim 2, wherein the support rod (24) is a rotary telescopic structure, and the length of the support rod can be adjusted.

4. The variable gravity cell experiment device based on three-dimensional rotation according to claim 2, wherein the monitoring control unit (3) comprises an acceleration sensor (31) and a controller (32);

the acceleration sensor (31) is arranged on the bottom surface of the cone support (26), can keep synchronous rotation with a cell culture bottle placed on the object placing plate (25), monitors the acceleration applied to the cell culture bottle at any time, and is connected with the controller (32) through radio;

the controller (32) is respectively connected with the first motor (14) and the second motor (17).

5. The variable gravity cell experiment device based on three-dimensional rotation as claimed in claim 1, wherein the insections on the outer side surface of the rotating shaft (12) are helical teeth and are in contact fit with the insections on the spur gear shaft (16); one end of the straight gear shaft (16) is connected with the diameter-winding rotating ring (11) through a shaft sleeve, and the other end of the straight gear shaft is connected with a second motor (17);

the front side surface and the rear side surface of the pivoting ring (12) are provided with annular chutes;

and a guide ring groove is arranged on the inner side of the diameter-winding rotating ring (11).

6. The variable gravity cell experiment device based on three-dimensional rotation as claimed in claim 5, wherein the anchoring pulley (15) comprises a U-shaped frame (151) with a downward opening, a shaft rod (152) vertically fixed at the middle position of the upper end of the outer part of the top plate of the U-shaped frame (151) and two balls (154) arranged on the inner walls of two side plates (153) of the U-shaped frame (151);

the shaft lever (152) is fixed in a guide ring groove of the radial rotating ring (11);

the balls (154) are limited between the annular sliding groove of the pivoting ring (12) and the inner wall of the side plate (153) of the U-shaped frame (151), the pivoting ring (12) is limited between the two balls (154) on the inner wall of the two side plates (153) of the U-shaped frame (151), the pivoting ring (12) slides between the two balls (154) of the anchoring pulley (15), and circumferential rotation of the pivoting ring (12) and the radial rotation ring (11) can be guaranteed while synchronous radial rotation is achieved.

7. The variable gravity cell experiment device based on three-dimensional rotation according to claim 6, wherein the number of the anchor pulleys (15) is four, and four anchor pulleys (15) are provided at the quarter positions of the rotating shaft (12).

8. The variable gravity cell experiment device based on three-dimensional rotation as claimed in claim 1, wherein the inner side of the rotating ring (12) is provided with a plurality of screw holes for installing a plurality of cell constant force units (2) inside the rotating ring (12).

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