CN209945832U - Paraffin embedding device for tissue positioning and embedding - Google Patents
Paraffin embedding device for tissue positioning and embedding Download PDFInfo
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- CN209945832U CN209945832U CN201920058305.6U CN201920058305U CN209945832U CN 209945832 U CN209945832 U CN 209945832U CN 201920058305 U CN201920058305 U CN 201920058305U CN 209945832 U CN209945832 U CN 209945832U
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Abstract
The utility model relates to a paraffin embedding device for tissue location embedding, it includes the embedding mould and seals the glue base, seal the glue base and include the bottom plate and locate the curb plate of bottom plate both sides, the bottom plate of sealing glue base one end is equipped with the recess that link up bottom plate width direction, the both ends inner wall fixed mounting of recess has the blade that the cutting edge exposes the bottom plate, be equipped with the pivot of horizontal seal glue base on the curb plate at recess both ends, still be equipped with the elasticity preforming on the curb plate of pivot top one side, be equipped with the fixing base on the both sides board of sealing the glue base other end, be equipped with the through-hole on the fixing base on the board of both sides, the back shaft of horizontal seal glue base is installed to the through-hole interlude formula, be equipped with the PET scotch tape on the back shaft, the embedding mould is the box body of. The utility model provides a traditional mould because paraffin solidifies too fast at the embedding in-process, leads to the problem of unable correct embedded knee joint.
Description
Technical Field
The utility model relates to an embedding technical field especially relates to a paraffin embedding device for tissue location embedding for tissue section's appearance piece carries out the embedding and handles.
Background
The tissue paraffin section has wide application in medical research, and the technology can observe normal tissue cell morphological structure and is an important reference basis for judging the cell histological morphological change. In the paraffin section technology, the paraffin embedding of tissues influences the final section quality, and bone tissues, skin tissues, vascular tissues, uterine tissues and other tissues with strict position requirements for embedding particularly need to be carefully controlled in the embedding process.
Taking bone tissue as an example, observation of knee joint tissue of animals is a main means for studying cartilage tissue structure and osteoarthritis pathology. The step of observing the animal knee joint tissues comprises the following steps: knee joint specimens are sectioned, sliced, stained or immunohistochemically observed under a microscope or fluorescence microscope. The number and quality of the slices are the basis of tissue observation, and the angle and position of the slices determine the accuracy and reliability of experimental data, even the success or failure of the experiment.
At present, the knee joint embedding modes are mainly divided into two types, one is sagittal embedding, and the other is coronal embedding. The coronal embedding method can better compare the pathological development process of the inner cartilage and the outer cartilage of the knee joint, and the coronal embedding method is mainly used for carrying out section analysis on the knee joint of the animal internationally at present. However, the adoption of the traditional mold for crown embedding has many disadvantages:
(1) the angle fixing time is short during embedding, the angle is not fixed well often, the bottom layer wax liquid is solidified, and only can be heated and dissolved again to be embedded again, so that the experimental efficiency is seriously influenced;
(2) after the angle is fixed, because the tissue is not firmly fixed by the bottom layer of wax liquid, the fixed tissue is easy to move in the moving process, so that the previous work is abandoned, and the tissue needs to be embedded again, thereby seriously affecting the experimental efficiency;
(3) because use traditional mould can't directly perceivedly see the relation of knee joint and horizontal plane, make the embedding angle can't accurately be mastered, can only rely on experience embedding, when leading to paraffin section afterwards, because the knee joint angle is not good leads to section quantity not enough, and the quality is not good, can't carry out observation and mark afterwards, often need to embed the wax stone again, extravagant sample when serious makes the experiment done its way to the utmost.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model provides a convenient to use's paraffin embedding device for organizing location embedding has solved traditional mould because unable comprehensive observation tissue fixed angle in the embedding process, and paraffin solidifies at the excessive speed simultaneously, leads to the problem of unable correct embedding knee joint, has improved work efficiency.
The utility model provides a technical scheme that its technical problem adopted is: the paraffin embedding device for tissue positioning embedding comprises an embedding mold and a sealing adhesive base, wherein:
the glue sealing base comprises a bottom plate and side plates arranged on two sides of the bottom plate, a groove penetrating through the width direction of the bottom plate is formed in the bottom plate at one end of the glue sealing base, blades with cutting edges exposed out of the bottom plate are fixedly arranged on the inner walls of two ends of the groove, a rotating shaft transversely arranged on the glue sealing base is arranged on the side plates at two ends of the groove, and an elastic pressing sheet is further arranged on the side plate on one side above the rotating shaft;
fixing seats are arranged on two side plates at the other end of the glue sealing base, through holes are formed in the fixing seats on the two side plates, a supporting shaft transversely arranged on the glue sealing base is installed in the through holes in an inserting mode, a PET (polyethylene terephthalate) transparent adhesive tape is arranged on the supporting shaft, and the width of the PET transparent adhesive tape is equal to that of the bottom plate;
the embedding mould is a box body with a hollowed-out top and a hollowed-out bottom, the length of the embedding mould is smaller than the length of the groove by 5-20 mm, and the width of the embedding mould is equal to that of the bottom plate.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the bottom plate and the side plate of the sealing glue base are integrally formed.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the distance from the upper contour line of the rotating shaft to the bottom plate is not less than the distance from the cutting edge of the blade to the bottom plate.
The utility model provides a paraffin embedding device for organize location embedding in a preferred embodiment, the both ends of recess still are equipped with the elastomer in the inboard of blade, the elastomer includes the film of "L" type and locates the arc guide plate of film top to recess incurve, the film adopts spring steel integrated into one piece of bending with the guide plate.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the two ends of the supporting shaft are provided with bolt holes, and the supporting shaft is fixed to the fixing base through the bolt.
The utility model provides a paraffin embedding device for organize location embedding in a preferred embodiment, the bottom plate of fixing base below is established to the formula structure that sinks, and the face that sinks is connected with the smooth transition of bottom plate, just through-hole to the distance of the face that sinks on the fixing base make the PET scotch tape use extremely not be higher than the last contour line of pivot when spacing.
The utility model provides a paraffin embedding device for tissue location embedding's a preferred embodiment, still overlap in the pivot and be equipped with anti-skidding cover.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the elastic pressing sheet is made of spring steel.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the embedding mold is made of stainless steel, and the height of the embedding mold is not less than the height of the blade.
In a preferred embodiment of the paraffin embedding device for tissue positioning and embedding provided by the present invention, the inner wall of the embedding mold is coated with a teflon coating.
Compared with the prior art, the utility model provides a paraffin embedding device for organize location embedding's beneficial effect is: the utility model discloses design the embedding mould into the box body structure of top and bottom fretwork, utilize and glue the glue base and glue one deck PET scotch tape and seal in the bottom of embedding mould, the tissue of treating the embedding is placed and is fixed and adjust the position after on the PET scotch tape and put the direct embedding of embedded wax liquid into the embedding mould, because embedding mould bottom is PET scotch tape, the top is the open type, the tissue is fixed convenient, and convenient the regulation, can observe the concrete position relation of tissue and embedding mould among the embedding process, can be high-efficient, fast, accurate embedding the tissue is good, very big improvement experimental efficiency, guarantee the embedding quality.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive work, wherein:
FIG. 1 is a schematic structural view of a paraffin embedding device for positioning and embedding tissues according to the present invention;
FIG. 2 is a top view of the encapsulant base provided in FIG. 1;
fig. 3 is a cross-sectional structural view of the elastomer provided in fig. 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1 and fig. 2, the paraffin embedding apparatus 1 for tissue positioning and embedding includes an embedding mold 11 and an encapsulation base 12, wherein:
the glue sealing base 12 comprises a bottom plate 121 and side plates 122 arranged on two sides of the bottom plate, preferably, the bottom plate 121 and the side plates 122 of the glue sealing base 12 are integrally formed, the bottom plate 121 at one end of the glue sealing base 12 is provided with a groove 1210 penetrating through the width direction of the bottom plate, blades 13 with cutting edges exposing out of the bottom plate are fixedly arranged on the inner walls of two ends of the groove 1210, rotating shafts 14 transversely arranged on the glue sealing base are arranged on the side plates 122 at two ends of the groove 1210, specifically, the distance from an upper contour line of the rotating shafts 14 to the bottom plate is not less than the distance from the cutting edges of the blades 13 to the bottom plate, an elastic pressing sheet 15 is further arranged on the side plate above the rotating shafts 14, preferably, the elastic pressing sheet 15 is made of spring steel, and the PET transparent adhesive tape is;
embedding mould 11 is the box body of top and bottom fretwork, just the length of embedding mould 11 is less than 5 ~ 20mm of recess 1210 length, and the width equals the width of bottom plate 121, guarantees that the bottom of embedding mould can be sealed by PET scotch tape completely, preferably, embedding mould 11 adopts stainless steel to make, and embedding mould 11 highly is not less than blade 13's height.
In a further improvement of this embodiment, the inner wall of the embedding mold 11 is coated with a teflon coating (not shown in the figure), and the non-adhesiveness of the teflon coating is utilized to realize the rapid removal of the embedded wax block.
For further improvement of this embodiment, referring to fig. 3, elastic bodies 19 are further disposed at two ends of the groove 1210 on the inner side of the blade, the elastic bodies 19 include an L-shaped bottom plate 191 and an arc-shaped guide plate 192 disposed at the top of the bottom plate and bending toward the groove, the bottom plate 191 and the guide plate 192 are integrally formed by bending spring steel, and the elastic bodies 19 are used to attach the PET transparent adhesive tape 18 cut off by the blade 13 to the outer walls of the two ends of the embedding mold 11, so as to ensure that the PET transparent adhesive tape is firmly attached to the embedding mold.
In a further improvement of this embodiment, the bottom plate below the fixing base 123 is a sinking structure, the sinking surface is in smooth transition connection with the bottom plate 121, and the distance from the through hole 1230 on the fixing base 123 to the sinking surface makes the PET scotch tape 18 not higher than the upper contour line of the rotating shaft 14 when the PET scotch tape is used to a limit position, so that the PET scotch tape is conveniently fixed on the rotating shaft.
In a further improvement of this embodiment, the rotating shaft 18 is further sleeved with an anti-slip sleeve (not shown in the figure), which is beneficial to fixing the elastic pressing sheet 15 to the PET transparent adhesive tape 18 and preventing the embedding mold 11 from loosening when pressed down.
During embedding: firstly, pulling out the PET transparent adhesive tape at one end of the adhesive sealing base and fixing the PET transparent adhesive tape on a rotating shaft, pressing down the bottom of the embedding mold above the groove to cut off the PET transparent adhesive tape under the action of a blade, and continuously pressing down, wherein two ends of the PET transparent adhesive tape are adhered to and covered on the outer walls of two ends of the embedding mold under the action of an elastic body to finish the sealing of the bottom PET transparent adhesive tape of the embedding mold;
then, place the tissue after conventional fixed, dehydration, wax-dipping treatment on the PET pellucid adhesive tape of embedding mould bottom with tweezers, because the PET pellucid adhesive tape has viscidity, can live the tissue bonding, adjust tissue and mould bottom sticky tape position relation according to the experiment needs during the placement, until reaching the section requirement, then to imbedding the built-in wax liquid of embedding mould (in the embedding process, the top of embedding mould is the opening, the bottom is transparent sticky tape, so can observe the concrete position relation of tissue at the embedding mould in the embedding process, can be high-efficient, quick, accurate with tissue embedding), after the wax liquid solidifies, tear the PET pellucid adhesive tape of embedding mould bottom, it can to take out slicer section on the wax block.
The embedding process does not need an embedding machine, so that the cost is saved while the experimental efficiency is improved.
The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes made by the present specification can be changed, or directly or indirectly applied to other related technical fields, and all the same principles are included in the protection scope of the present invention.
Claims (10)
1. The utility model provides a paraffin embedding device for tissue location embedding which characterized in that: including embedding mould and the base of gluing, wherein:
the glue sealing base comprises a bottom plate and side plates arranged on two sides of the bottom plate, a groove penetrating through the width direction of the bottom plate is formed in the bottom plate at one end of the glue sealing base, blades with cutting edges exposed out of the bottom plate are fixedly arranged on the inner walls of two ends of the groove, a rotating shaft transversely arranged on the glue sealing base is arranged on the side plates at two ends of the groove, and an elastic pressing sheet is further arranged on the side plate on one side above the rotating shaft;
fixing seats are arranged on two side plates at the other end of the glue sealing base, through holes are formed in the fixing seats on the two side plates, a supporting shaft transversely arranged on the glue sealing base is installed in the through holes in an inserting mode, a PET (polyethylene terephthalate) transparent adhesive tape is arranged on the supporting shaft, and the width of the PET transparent adhesive tape is equal to that of the bottom plate;
the embedding mould is a box body with a hollowed-out top and a hollowed-out bottom, the length of the embedding mould is smaller than the length of the groove by 5-20 mm, and the width of the embedding mould is equal to that of the bottom plate.
2. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the bottom plate and the side plate of the glue sealing base are integrally formed.
3. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the distance from the upper contour line of the rotating shaft to the bottom plate is not less than the distance from the cutting edge of the blade to the bottom plate.
4. The paraffin embedding device for tissue localization embedding of claim 1, wherein: elastic bodies are further arranged at the two ends of the groove and on the inner sides of the blades, each elastic body comprises an L-shaped bottom sheet and an arc-shaped guide sheet arranged at the top of the bottom sheet and bent towards the groove, and the bottom sheet and the guide sheet are integrally formed by bending spring steel.
5. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the both ends of back shaft are equipped with the bolt hole, the back shaft is fixed in the fixing base through the bolt.
6. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the bottom plate below the fixing seat is of a sinking structure, the sinking surface is connected with the bottom plate in a smooth transition mode, and the distance from the through hole in the fixing seat to the sinking surface enables the PET transparent adhesive tape to be not higher than the upper contour line of the rotating shaft when the PET transparent adhesive tape is used to be limited.
7. The paraffin embedding device for tissue localization embedding of claim 1, wherein: and the rotating shaft is also sleeved with an anti-skidding sleeve.
8. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the elastic pressing sheet is made of spring steel.
9. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the embedding mould is made of stainless steel materials, and the height of the embedding mould is not less than that of the blade.
10. The paraffin embedding device for tissue localization embedding of claim 1, wherein: the inner wall of the embedding mould is coated with a Teflon coating.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920058305.6U CN209945832U (en) | 2019-01-15 | 2019-01-15 | Paraffin embedding device for tissue positioning and embedding |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920058305.6U CN209945832U (en) | 2019-01-15 | 2019-01-15 | Paraffin embedding device for tissue positioning and embedding |
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| CN209945832U true CN209945832U (en) | 2020-01-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109781494A (en) * | 2019-01-15 | 2019-05-21 | 中南大学湘雅医院 | Paraffin embedding device and its embedding method for organizing positioning to embed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109781494A (en) * | 2019-01-15 | 2019-05-21 | 中南大学湘雅医院 | Paraffin embedding device and its embedding method for organizing positioning to embed |
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