CN209934986U

CN209934986U - PHA draws with extract product centrifugation purification device

Info

Publication number: CN209934986U
Application number: CN201920037420.5U
Authority: CN
Inventors: 邱荣昇; 邱静宜; 詹前埔
Original assignee: Guangzhou Yuanyan Biotechnology Co Ltd
Current assignee: Guangzhou Yuanyan Biotechnology Co Ltd
Priority date: 2019-01-10
Filing date: 2019-01-10
Publication date: 2020-01-14
Anticipated expiration: 2029-01-10

Abstract

The utility model belongs to centrifugation purification field, especially a PHA draws uses extract product centrifugation purification device, to the inconvenient cleanness that goes on of current centrifugation purification mechanism, the inconvenient problem of collecting the extract product simultaneously, the following scheme of present proposition, it is installed in the rotor plate that supports the base top including supporting the base and rotating, the rotating electrical machines is installed to one side of supporting the base, and the output shaft of rotating electrical machines has drive gear, and the lateral wall of rotor plate is equipped with the outer ring gear with drive gear meshing, the centre gripping motor is installed to the top intermediate position of rotor plate, and the output shaft of centre gripping motor passes the rotor plate and is connected with the driving gear, it has a plurality of rotation holes to open on the rotor plate, rotates downthehole rotation and is connected with fixture. This device can realize conveniently carrying out the centre gripping to the charging barrel fixedly to soybean PHA's centrifugal purification, conveniently unloads, then the charging barrel cleans.

Description

PHA draws with extract product centrifugation purification device

Technical Field

The utility model relates to a centrifugation purification technical field especially relates to a PHA draws and uses extract product centrifugation purification device.

Background

Phytohemagglutinin (PHA) is a lectin found in plants, particularly in leguminous plants, and belongs to the class of high molecular glycoproteins, and is a complex of oligosaccharides (composed of galactose, N-acetylglucosamine and mannose) and proteins, and has mitogenic and interferon-secretion inducing activities. PHA is a tetrameric glycoprotein formed by 4 subunits bound by non-covalent bonds, comprising two subunit molecules, L subunit (leukolectin) and E (hemagglutinin), and thus there are 5 isomers, L4, L3E1, L2E2, L1E3 and E4, respectively. The L subunit has leukocyte agglutination and high mitogenic activity; the E subunit has high erythrocyte aggregation and low mitogenic activity.

Currently, PHA is available in the form of PHA-L, PHA-E, PHA-M, PHA-P, etc. PHA-L is purified L4, has the main function of serving as a stimulator of T lymphocytes, is widely used for immunological research (such as serving as a positive control of INF-gamma ICS and ELISPOT experiments; or serving as a stimulator of PBMC culture), and can also serve as an antegrade nerve tracer; PHA-E is purified E4, and the main application is the research of erythrocyte agglutination or glycosylation modification. PHA-P is a protein form of PHA before PHA-L and PHA-E are separated and purified, PHA-M is a viscous protein form of phytohemagglutinin, and the two components are equivalent, and are mainly used for stimulating the proliferation of peripheral mononuclear cells, promoting the production of certain cytokines and the expression of membrane surface proteins.

The existing centrifugal purification mechanism is inconvenient to clean and collect the extract, so that the centrifugal purification device for the extract for PHA extraction is provided.

SUMMERY OF THE UTILITY MODEL

The utility model provides a PHA draws uses extract product centrifugation purification device has solved the inconvenient cleanness that cleans of current centrifugation purification mechanism, the inconvenient problem of collecting the extract product simultaneously.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a centrifugal purification device for PHA extraction extract comprises a support base and a rotating plate rotatably mounted at the top end of the support base, wherein a rotating motor is mounted at one side of the support base, an output shaft of the rotating motor is connected with a driving gear, an outer gear ring meshed with the driving gear is arranged on the outer side wall of the rotating plate, a clamping motor is mounted at the middle position of the top end of the rotating plate, an output shaft of the clamping motor penetrates through the rotating plate and is connected with the driving gear, a plurality of rotating holes are formed in the rotating plate, and a clamping mechanism is rotatably connected in the rotating holes;

the fixture is including rotating the column spinner of connection in the rotation hole, and the bottom of column spinner extends to the below of rotor plate and is connected with the driven gear with driving gear meshing, column spinner threaded connection has the rotating ring, and the top of rotating ring is rotated and is connected with the loose ring of cup jointing on the column spinner, and articulated on the lateral wall of loose ring have a plurality of connecting rods, the top of column spinner is fixed with the turning block, and the border department of turning block articulates there is the transfer line, and the bottom of transfer line articulates there is the connecting rod, and the top of transfer line articulates there is the grip block, and the intermediate position clamp of a plurality of grip blocks has the section of thick bamboo that is located the turning block.

Preferably, a liquid extraction mechanism is mounted on the side wall of the support base.

Preferably, the liquid extraction mechanism comprises a support frame welded on a support base, the top end of the support frame extends to the position above the clamping mechanism, a liquid pump and a mounting frame are installed on the side wall of the top end of the support frame, the input end of the liquid pump is connected with a discharging pipe, a movable hole between the liquid pump and the mounting frame is formed in the side wall of the top end of the support frame, a movable column is connected in a sliding manner in the movable hole, a suction hole is formed in the movable column in the length direction of the movable column and is connected with the input end of the liquid pump through a guide pipe, a mounting groove formed in the side wall of the movable column is formed in one side of the suction hole, a rack is installed in the mounting groove, a rotating handle is connected to the mounting frame in a rotating manner, one end of the rotating handle penetrates through the mounting frame and is, the bolt is sleeved with a spring in a sliding manner, and two ends of the spring are connected to the bolt and the fixing block respectively.

Preferably, a placing groove is formed in the top end of the rotating block, and the bottom end of the charging barrel is clamped in the placing groove.

Preferably, the bottom of activity ring is opened has annular spread groove, and the top of rotating ring is equipped with two sliders, and slider sliding connection is in annular spread groove, and the cross-section of slider is the T shape.

Preferably, the top of supporting the base is equipped with annular slider, and open the bottom of rotor plate has annular spout, and annular slider sliding connection is in annular spout.

Preferably, the cross section of the clamping plate is arc-shaped.

Preferably, a bearing is installed at the joint of the rotating column and the rotating plate.

The utility model has the advantages that: through the arrangement of the clamping mechanism, the charging barrel can be clamped and fixed conveniently, discharging is facilitated, and then the charging barrel is cleaned;

through the arrangement of the outer gear ring, the clamping mechanism, the rotating plate, the clamping motor, the driving gear, the supporting base, the driven gear, the rotating motor and the driving gear, the centrifugal purification of the soybean PHA is facilitated;

the purified soybean PHA is conveniently unloaded by arranging the liquid extraction mechanism;

this device can realize conveniently carrying out the centre gripping to the charging barrel fixedly to soybean PHA's centrifugal purification, conveniently unloads, then the charging barrel cleans.

Drawings

FIG. 1 is a schematic structural view of an extract centrifugal purifying device for PHA extraction according to the present invention.

FIG. 2 is a schematic structural view of a clamping mechanism of the centrifugal purification device for PHA extraction.

FIG. 3 is a schematic view of a partial structure of a centrifugal purification apparatus for PHA extraction.

Reference numbers in the figures: the device comprises an outer gear ring 1, a clamping mechanism 2, a movable ring 21, a rotary column 22, a rotary ring 23, a rotary block 24, a connecting rod 25, a transmission rod 26, a placement groove 27, a clamping plate 28, a charging barrel 29, a rotary plate 3, a clamping motor 4, a driving gear 5, a supporting base 6, a driven gear 7, a rotary motor 8, a driving gear 9, a supporting frame 10, a discharging pipe 11, a liquid pump 12, a movable column 13, a rack 131, a suction hole 132, a transmission gear 14, a bolt 15, a fixed block 16, a spring 17, an installation frame 18 and a rotary handle 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a centrifugal purification device for PHA extraction extract comprises a supporting base with an opening at the top end and a rotating plate rotatably mounted at the top end of the supporting base, wherein a rotating motor is mounted at one side of the supporting base, an output shaft of the rotating motor is connected with a driving gear, an outer gear ring meshed with the driving gear is arranged on the outer side wall of the rotating plate, a clamping motor is mounted at the middle position of the top end of the rotating plate, an output shaft of the clamping motor penetrates through the rotating plate and is connected with the driving gear, a plurality of rotating holes are formed in the rotating plate, and a clamping mechanism is rotatably connected in the;

the clamping mechanism comprises a rotary column which is rotatably connected in a rotary hole, the bottom end of the rotary column extends to the lower part of a rotary plate and is connected with a driven gear which is meshed with a driving gear, the rotary column is in threaded connection with a rotary ring, the top end of the rotary ring is rotatably connected with a movable ring which is sleeved on the rotary column, the outer side wall of the movable ring is hinged with a plurality of connecting rods, the top end of the rotary column is fixed with a rotary block, the edge of the rotary block is hinged with a transmission rod, the bottom end of the transmission rod is hinged with a connecting rod, the top end of the transmission rod is hinged with a clamping plate, the middle positions of the plurality of clamping plates clamp a charging barrel which is positioned at the top end of the rotary block, the side wall of the supporting base is provided with a liquid extraction mechanism, the liquid extraction mechanism comprises a supporting frame which is welded, the input end of the liquid pump is connected with a discharging pipe, the side wall of the top end of the supporting frame is provided with a movable hole positioned between the liquid pump and the mounting frame, the movable hole is connected with a movable column in a sliding way, the movable column is provided with a suction hole along the length direction thereof, the suction hole is connected with the input end of the liquid pump through a guide pipe, one side of the suction hole is provided with a mounting groove arranged on the side wall of the movable column, a rack is arranged in the mounting groove, the mounting frame is connected with a rotating handle in a rotating way, one end of the rotating handle penetrates through the mounting frame to be connected with a transmission gear, the transmission gear is meshed with the rack, the mounting frame is fixed with a fixed block, the fixed block is connected with a bolt in a sliding way, one end of the bolt is clamped on the transmission gear, a spring is sleeved on the bolt in a sliding way, the, the top of swivel becket is equipped with two sliders, and slider sliding connection is in annular spread groove, and the cross-section of slider is T shape, and the top of supporting the base is equipped with annular slider, and open the bottom of rotor plate has annular spout, and annular slider sliding connection is in annular spout, and the cross section of grip block is the arc, and the bearing is installed to the junction of column spinner and rotor plate.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Example (b): when in use, a plurality of charging barrels 29 are respectively placed in the placing grooves 27 on the rotating block 24, the rotating ring 23 on each clamping mechanism 2 is rotated, the rotating ring 23 is in threaded connection with the rotating column 22 to drive the movable ring 21 to move downwards, the movable ring 21 drives the transmission rod 26 to rotate around the edge of the rotating block 24 through the connecting rod 26 to drive the clamping plate 28 to clamp and fix the charging barrels 29, soybean solution to be centrifuged is added into the charging barrels 29, additives are added and uniformly stirred, the clamping motor 4 drives the driving gear 5 to rotate, the driving gear 5 drives the rotating column 22 to rotate through the driven gear 7, the output shaft of the rotating motor 8 drives the rotating plate 3 to rotate on the supporting base 6 through the driving gear 9 and the external gear ring, centrifugal purification is carried out for 20 minutes at 10000 rotation speeds per hour, after centrifugation is finished, the rotating plate 3 is rotated to drive the charging barrels 29 to move to the lower part of the movable column 13, pulling the bolt 15 to drive the spring 17 to compress, wherein one end of the bolt 15 is not meshed with the transmission gear 14, rotating the rotating handle 19 to drive the transmission gear 14 to rotate, the transmission gear 14 is meshed with the rack 131 to drive the movable column 13 to move downwards to the lowest part of the supernatant in the centrifugate, starting the liquid pump 12 to pump out the supernatant to obtain the extracting solution of the soybean PHA, reversely rotating the rotating ring 23 on each clamping mechanism 2, the rotating ring 23 is in threaded connection with the rotating column 22 to drive the movable ring 21 to move upwards, the movable ring 21 drives the transmission rod 26 to rotate around the edge of the rotating block 24 through the connecting rod 26 to drive the clamping plate 28 not to clamp and fix the charging barrel 29, taking out the residual materials in the charging barrel 29 and pouring the residual materials into a designated container, and then conveniently cleaning the charging barrel 29, thereby facilitating the next operation, the device can realize the centrifugal purification of the soybean PHA and conveniently clamp and fix the charging barrel, conveniently unload, then the charging barrel cleans.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The centrifugal purification device for the extract for PHA extraction comprises a support base (6) and a rotating plate (3) rotatably mounted at the top end of the support base (6), and is characterized in that a rotating motor (8) is mounted on one side of the support base (6), an output shaft of the rotating motor (8) is connected with a driving gear (9), an outer gear ring (1) meshed with the driving gear (9) is arranged on the outer side wall of the rotating plate (3), a clamping motor (4) is mounted at the middle position of the top end of the rotating plate (3), an output shaft of the clamping motor (4) penetrates through the rotating plate (3) to be connected with a driving gear (5), a plurality of rotating holes are formed in the rotating plate (3), and clamping mechanisms (2) are rotatably connected in the rotating holes;

the clamping mechanism (2) comprises a rotary column (22) which is rotatably connected in the rotary hole, the bottom end of the rotary column (22) extends to the lower part of the rotary plate (3) and is connected with a driven gear (7) which is meshed with the driving gear (5), the rotating column (22) is in threaded connection with a rotating ring (23), the top end of the rotating ring (23) is rotatably connected with a movable ring (21) sleeved on the rotating column (22), the outer side wall of the movable ring (21) is hinged with a plurality of connecting rods (25), the top of the rotating column (22) is fixed with a rotating block (24), the edge of the rotating block (24) is hinged with a transmission rod (26), the bottom end of the transmission rod (26) is hinged with a connecting rod (25), the top end of the transmission rod (26) is hinged with a clamping plate (28), and the middle positions of the clamping plates (28) clamp a charging barrel (29) positioned at the top end of the rotating block (24).

2. -extract centrifugal refining device for PHA extraction according to claim 1, characterized in that on the side wall of the supporting base (6) liquid extraction means are mounted.

3. The centrifugal purification device for PHA extraction extract as claimed in claim 2, wherein the liquid extraction mechanism comprises a support frame (10) welded on a support base (6), the top end of the support frame (10) extends above the clamping mechanism (2), a liquid pump (12) and a mounting frame (18) are mounted on the side wall of the top end of the support frame (10), the input end of the liquid pump (12) is connected with a discharge pipe (11), the side wall of the top end of the support frame (10) is provided with a movable hole positioned between the liquid pump (12) and the mounting frame (18), a movable column (13) is connected in the movable hole in a sliding manner, the movable column (13) is provided with a suction hole (132) along the length direction thereof, the suction hole (132) is connected with the input end of the liquid pump (12) through a conduit, one side of the suction hole (132) is provided with a mounting groove formed on the side wall of the movable, install rack (131) in the mounting groove, it is connected with turning handle (19) to rotate on mounting bracket (18), and the one end of turning handle (19) is passed mounting bracket (18) and is connected with drive gear (14), and drive gear (14) and rack (131) meshing, be fixed with fixed block (16) on mounting bracket (18), sliding connection has bolt (15) on fixed block (16), and the one end joint of bolt (15) is on drive gear (14), and sliding sleeve has been connected spring (17) on bolt (15), and the both ends of spring (17) are connected respectively on bolt (15) and fixed block (16).

4. The centrifugal purification device of PHA extracts of claim 1, wherein the top end of said rotating block (24) is provided with a placement groove (27), and the bottom end of the charging barrel (29) is clamped in the placement groove (27).

5. The centrifugal purification device of extract for PHA extraction according to claim 1, wherein the bottom end of said movable ring (21) is provided with an annular connecting groove, the top end of the rotating ring (23) is provided with two sliding blocks which are slidably connected in the annular connecting groove, and the cross section of the sliding block is T-shaped.

6. The centrifugal purification device of PHA extracts of claim 1, wherein the top of the supporting base (6) is equipped with an annular slide block, the bottom of the rotating plate (3) is equipped with an annular slide slot, and the annular slide block is slidably connected in the annular slide slot.

7. The centrifugal purification device of extract for PHA extraction according to claim 1, wherein the cross section of the holding plate (28) is arc-shaped.

8. -extract centrifugal purification device for PHA extraction according to claim 1, characterized in that the junction of the rotating column (22) and the rotating plate (3) is equipped with bearings.