CN209928126U - Uniform light microscopic lighting device based on Mie scattering - Google Patents
Uniform light microscopic lighting device based on Mie scattering Download PDFInfo
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- CN209928126U CN209928126U CN201921019213.3U CN201921019213U CN209928126U CN 209928126 U CN209928126 U CN 209928126U CN 201921019213 U CN201921019213 U CN 201921019213U CN 209928126 U CN209928126 U CN 209928126U
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Abstract
The utility model relates to an illumination technical field with miniaturized low-power consumption light source specifically is an even irradiant lighting device and method based on mie scattering, is applied to microscope light source field, has solved the problem that traditional even light source realizes that the structure is complicated, miniaturized difficulty, product price are expensive. The homogeneous light microscopic illuminator based on Mie scattering includes light source, Mie scattering device, optical cavity and collecting lens. The Mie scattering device is a solid transparent or semitransparent optical device distributed with Mie scattering medium particles, and is arranged in the optical cavity, and light emitted by the light source forms uniform irradiation light through the Mie scattering device and is intensively irradiated on the focus of the condenser lens through the condenser lens. The utility model discloses simple structure and miniaturization, low power dissipation, illumination are even, stable performance, long service life, with low costs, are applicable to the microscope light source, especially small-size optical microscope light source field.
Description
Technical Field
The utility model relates to an illumination technology field with miniaturized low-power consumption light source specifically is an even illumination's lighting device and method based on mie scattering, is applied to microscope light source field.
Background
The light source system of the optical microscope is characterized in that the light irradiated by the light source system is generally in Gaussian distribution, the brightness of the central area of a microscopic field is highest, the brightness of the periphery of the microscopic field is lower, and the problem of uneven illumination is reflected in a digital microscope that digital images show uneven brightness and darkness, especially in the field of digital pathology full-section imaging. In general, digital pathology images are large in size, and performing illumination compensation on such large data is computationally inefficient, resulting in digital pathology imaging that is time consuming overall.
In order to solve the above problems, high-end microscope systems typically employ a specially designed illumination lens, typically kohler illumination, which utilizes a condenser lens, a field stop, a condenser stop, and a condenser lens, arranged in order between a light source and a sample, to produce very uniform sample illumination and ensure that adverse effects due to uneven illumination light distribution are not visible in the image. However, such an illumination optical structure is complicated, and the use of more lenses increases the cost, which is disadvantageous for the miniaturization and cost reduction of the illumination system.
Disclosure of Invention
In order to solve the uneven problem of above-mentioned micro-illumination, realize miniaturized, with low costs, low power dissipation, long service life's microscope illumination, the utility model provides a micro-lighting device of even light based on mie scattering.
The technical principle of the utility model is as follows: a homogeneous light microscopic lighting device based on Mie scattering is characterized by that its core device is a solid optical device full of Mie scattering medium particles, said device is a transparent or semitransparent solid made of optical resin or optical glass, and has the property of making incident light beam implement uniform scattering output. And a light source and a condenser are respectively arranged on two sides of the Mie scattering device, one end of the light source is a light beam incidence end, and the other end of the condenser is a light beam output end. In order to prevent incident light from transmitting outwards from the Mie scattering device after entering the Mie scattering device, the hollow lens barrel is wrapped outside the Mie scattering device, in order to improve the illumination utilization rate of a light source, the inner wall of the lens barrel can be subjected to total reflection film coating treatment, and in order to further reduce the cost and under the condition of enough illumination brightness, the inner wall of the lens barrel can be subjected to black oxidation treatment.
After the light beam emitted by the light source irradiates the Mie scattering device, because the interior of the device is filled with medium particles, a large amount of Mie scattering occurs when the light beam meets the particles, and the scattered light is continuously scattered and superposed in the process of passing through the Mie scattering device, and finally, the light with uniform illumination is output.
When the inner wall of the lens cone is a total reflection coating, light scattered in the Mie scattering device is reflected by the inner wall of the lens cone and is scattered, superposed and reflected continuously in the period, so that the light intensity loss is less, and the light intensity output by the Mie scattering device is brighter.
When the inner wall of the lens cone is subjected to black oxidation treatment, light scattered in the Mie scattering period irradiates the inner wall of the lens cone, and is not reflected or is extremely weak, so that the loss of light transmitted in the Mie scattering device is large, and the light intensity output in the Mie scattering period is weak.
When the light source adopts a color light emitting diode, the red light emitting diode, the blue light emitting diode and the green light emitting diode can not be integrated at the same position due to the manufacturing process of the diodes, and three focuses at different positions are formed through a condenser lens, so that the color deviation occurs in microscopic observation. However, based on the mie scattering theory, the degree of mie scattering is independent of the wavelength, and the property after photon scattering remains regardless, so that, through the mie scattering device, stable and uniform white output is obtained although the three light emitting diodes are at different positions, and a focus can be formed through the condenser lens.
When the light source adopts a white light emitting diode, the spectral components of the white light emitted by each diode are different, but based on the above mie scattering theory, the white light output by the mie scattering device can form stable and uniform white light output despite the different light emitting diodes, and the stability of the light source is improved.
Compared with the prior art, the utility model, have following advantage:
(1) the utility model discloses the white light of output, the illumination is even, and spectral distribution is even, and traditional micro-light source illumination is bright and is gaussian distribution, and the spectral distribution of different illuminations is not necessarily the same.
(2) The utility model discloses can reduce through the light source volume, and can guarantee higher light source utilization ratio, traditional light source is bulky, and can not guarantee light source utilization ratio.
(3) The utility model discloses simple structure and assembly are simple, realize that the focus shines, only need a piece of optical lens, and traditional light source needs the combination of many pieces of lens to just can realize shining relatively even light source.
(4) The utility model discloses the light source adopts emitting diode, low power dissipation, low price, and can use more than 5 ten thousand hours continuously, consequently, for traditional light source, the utility model discloses stable, reliable, live time is long.
To sum up, the utility model discloses can realize miniaturized, with low costs, low power dissipation, the long service life even light micro-lighting device.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
Fig. 1 is a schematic structural view of a homogeneous light micro-lighting device based on mie scattering according to the present invention;
fig. 2 is a schematic light path diagram of a homogeneous light micro-lighting device based on mie scattering according to the present invention;
fig. 3 is a schematic view of the application of the homogeneous light microscope illumination device based on mie scattering in digital pathological microscopic imaging according to the present invention;
FIG. 4 is a microscopic image of a homogeneous light micro-illuminator based on Mie scattering according to the present invention;
fig. 5 is a microscopic image when illuminated using a conventional microscopic illumination apparatus.
Reference numerals: 101-a light source; 102-an optical cavity; 103-mie scattering devices; 104-mie scattering medium particles; 105-a condenser lens; 201-light emitted by a light source; 202-light scattered by mie scattering medium particles; 203-light scattered onto the optical cavity wall for reflection; 204-light emitted by the mie scattering device; 205-light collected by a condenser; 206-converging the light to form a light plane; 301-micro LED light emitting chip; 302-UV curing optical adhesive glue; 303-mie scattering micro-rods; 304-nanospheres; 305-transparent polycarbonate; 306-a lens barrel; 307-plano-convex lens; 308-outgoing light; 309-slide; 310-observed cell; 311-coverslip; 312-microscope objective; 402-image center bright using traditional illumination; 403-image dark all around using traditional lighting.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that, if directional indications (such as upper, lower, left, right, front and rear … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are changed accordingly.
The present invention will be described in further detail below with reference to a homogeneous light micro-lighting device based on mie scattering, but the scope of the present invention should not be limited thereto.
An object of the utility model is to provide a micro-lighting device of even light based on mie scattering for the even microscope observation of image background luminance or image acquisition, especially digital pathology whole section imaging provides a solution.
As shown in fig. 1, a homogeneous light microscope illumination device based on mie scattering includes a light source 101, a mie scattering device 103, an optical cavity 102, and a condenser lens 105. Among them, the light source 101 may be a miniaturized low power consumption light source. The light source 101 is located on one side of the mie scattering device 103 and the condenser lens 105 is located on the other side of the mie scattering device 103. The Mie scattering device 103 is nested within the optical cavity 102. The light source 101 is used to emit light onto the mie scattering device 103. The Mie scattering device 103 is used for scattering light emitted by the light source 101 to irradiate the condenser lens 105; the condenser lens 105 is used for focusing and outputting the light entering the mie scattering device 103.
Fig. 2 is a schematic light path diagram of the uniform light micro-lighting device based on mie scattering. The arrow 201 indicates light emitted from the light source 101, the arrow 202 indicates light scattered by the mie scattering medium particles 104, the arrow 203 indicates light scattered by the light and reflected by the optical cavity wall, the arrow 204 indicates light emitted from the mie scattering device 103, the arrow 205 indicates light condensed by the condenser lens 105, and the arrow 206 indicates a light irradiation surface formed by the condensed light.
The light source 101 may be a light emitting diode or a semiconductor laser. The mie scattering device 103 may be a solid optical device in which mie scattering medium particles 104 are distributed. The mie scattering medium particles 104 are distributed inside the solid optical device. The optical device is transparent, or translucent. The refractive index of the optics is smaller than the refractive index of the mie scattering medium particles 104. The optical cavity 102 may be a hollow cylindrical closed cavity with two open ends and wrapped outside the mie scattering device 103, and the inner wall of the optical cavity is a mirror surface for reflecting light or a black oxide layer surface. The condenser lens 105 is an optical lens or a combination of optical lenses that can condense and irradiate uniform light in a direction of propagation.
In a specific application example, as shown in fig. 3, the light source 101 may be a micro LED light emitting chip 301, the mie scattering device 103 is a mie scattering micro rod 303, the optical cavity 102 is a lens barrel 306, and the condenser lens 105 is a plano-convex lens 307. In this example, the homogeneous light micro-illumination device based on mie scattering includes a micro LED light emitting chip 301, a mie scattering micro rod 303, a lens barrel 306, and a plano-convex lens 307. The mie scattering micro-rod 303 is a small-sized cylindrical optical device with a transparent polycarbonate 305 as a base material, wherein lactic acid-glycolic acid Polymer (PLGA) or polyvinyl alcohol (PVA) is doped as the material, and the diameter of the nano-microsphere 304 is 1-100 nm, and the nano-microsphere 304 is the mie scattering medium particle 104. The diameter of the cylinder is the same as or similar to that of the LED light-emitting part, the refractive index of the polycarbonate is smaller than that of the nano microspheres, and the nano microspheres are distributed randomly, so that incident light can irradiate the nano microspheres, and Mie scattering occurs. The lens cone 306 is an opaque hollow cylinder wrapping the surface of the Mie scattering micro-rod 303, and the inner wall of the lens cone is coated with a light reflection mirror surface, so that Mie scattering light can be reflected, and emergent light is ensured to have stronger brightness. The plano-convex lens 307 is a lens having two surfaces, one surface is a plane, the other surface is a spherical surface, the emergent light of the mie scattering micro-rod 303 is converged, and the distance of a convergence point is equal to the focal distance of the plano-convex lens 307. The micro LED light-emitting chip 301 and the plano-convex lens 307 are respectively bonded to the input end and the output end of the Mie scattering micro-rod by using UV curing optical bonding glue 302, the light-emitting surface of the micro LED light-emitting chip 301 is bonded to the surface of the input end of the Mie scattering micro-rod 303, and the plane surface of the plano-convex lens 307 is bonded to the output end of the Mie scattering micro-rod 303.
An embodiment of the homogeneous light microscope illumination device based on mie scattering is used in the following way: as shown in fig. 3, the micro LED light emitting chip 301 is first powered on, the LED surface emits light, and the light beam is mie scattered inside the mie scattering micro rod 303, so that uniform white light is output from the mie scattering micro rod 303 and irradiated on the plane surface of the plano-convex lens 307, and the uniform white light, i.e. the emergent light 308 of the plane lens 307 is converged and irradiated on the focal point of the plano-convex lens 307 by refraction of the plano-convex lens 307. At this time, if the observed object is the observed cell 310 on the tissue section, the tissue section is located between the slide 309 and the cover glass 311. The tissue slice is positioned on the uniform white light convergence focus, the uniform white light penetrates through the tissue slice to form object light, the object light irradiates into a microscope objective 312 of an optical microscope, the object light is amplified and imaged on a digital image sensor through an optical system of the microscope, and the data of the digital image sensor is read and encoded through a computer to form a digital microscope image which is displayed on a display. Since the light irradiated on the tissue cells is white light uniformly irradiated, a background with different brightness and a gaussian distribution of the light emitted from the original LED cannot be observed on the digital microscopic image, and the background light is uniform.
Wherein, figure 4 is the use the utility model relates to a microscopic image when even light micro-lighting device based on mie scattering throws light on, figure 5 is the microscopic image when using the illumination of traditional micro-lighting device. In fig. 5, 402 indicates that the center of the image is bright when the conventional illumination is used, and 403 indicates that the periphery of the image is dark when the conventional illumination is used. By comparing fig. 4 and fig. 5, it can be seen that the white light output by the present invention has uniform illumination and uniform spectral distribution; the traditional microscopic light source has the illumination brightness in Gaussian distribution and uneven illumination distribution.
Here, it should be noted that: in the case of no conflict, a person skilled in the art may combine the related technical features in the above examples according to actual situations to achieve corresponding technical effects, and details of various combining situations are not described herein.
It is above only the utility model discloses a preferred embodiment, the utility model discloses a scope of protection does not only confine above-mentioned embodiment, the all belongs to the utility model discloses a technical scheme under the thinking all belongs to the utility model discloses a scope of protection. It should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A homogeneous light microscopic lighting device based on Mie scattering is characterized by comprising a light source, a Mie scattering device, an optical cavity and a condenser lens;
the optical cavity is provided with a Mie scattering device, a light source, a condenser and an optical cavity, wherein the light source is positioned on one side of the Mie scattering device, the condenser is positioned on the other side of the Mie scattering device, and the Mie scattering device is sleeved in the optical cavity; the light source is used for emitting light to irradiate the Mie scattering device; the Mie scattering device is used for scattering and irradiating light emitted by the light source onto the condenser; the condenser is used for focusing and outputting the light emitted by the Mie scattering device.
2. The Mie scattering based homogeneous light micro-illuminator according to claim 1, wherein the light source is a light emitting diode or a semiconductor laser.
3. The homogeneous light microscope illumination device based on mie scattering according to claim 1, wherein the mie scattering device is a solid optical device distributed with mie scattering medium particles, the optical device is transparent or semi-transparent, and the refractive index of the optical device is smaller than that of the mie scattering medium particles.
4. The homogeneous light microscopic illumination device based on mie scattering of claim 1, wherein the optical cavity is a hollow cylindrical closed cavity with two open ends and wrapped outside the mie scattering device, and the inner wall of the optical cavity is a mirror surface for reflecting light or a surface of a black oxide layer.
5. The Mie scattering-based uniform light microscope illumination device as claimed in claim 1, wherein the condenser is an optical lens or a combination of optical lenses capable of converging and illuminating the uniform light by contracting the propagation direction of the uniform light.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921019213.3U CN209928126U (en) | 2019-07-01 | 2019-07-01 | Uniform light microscopic lighting device based on Mie scattering |
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| CN201921019213.3U CN209928126U (en) | 2019-07-01 | 2019-07-01 | Uniform light microscopic lighting device based on Mie scattering |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110221422A (en) * | 2019-07-01 | 2019-09-10 | 达科为（深圳）医疗设备有限公司 | A kind of uniform light micro illumination device based on Mie scattering |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110221422A (en) * | 2019-07-01 | 2019-09-10 | 达科为（深圳）医疗设备有限公司 | A kind of uniform light micro illumination device based on Mie scattering |
| CN110221422B (en) * | 2019-07-01 | 2024-04-02 | 达科为（深圳）医疗设备有限公司 | Uniform light microscopic lighting device based on Mie scattering |
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