CN209923312U - Liquid separator and centrifugal liquid separating device - Google Patents
Liquid separator and centrifugal liquid separating device Download PDFInfo
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- CN209923312U CN209923312U CN201920182725.5U CN201920182725U CN209923312U CN 209923312 U CN209923312 U CN 209923312U CN 201920182725 U CN201920182725 U CN 201920182725U CN 209923312 U CN209923312 U CN 209923312U
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Abstract
The utility model relates to a cell separation technical field especially relates to a knockout and centrifugation divide liquid device, and wherein the knockout is including collecting the subassembly and dividing the liquid subassembly, and the liquid outlet of collecting the subassembly is connected with the inlet that divides the liquid subassembly, divides the bottom of liquid subassembly to have a plurality of liquid holes, and the axis that goes out the liquid hole is along dividing the interior liquid flow direction tilt up of liquid subassembly. The utility model discloses can once only fall cell suspensions such as dilution blood or homogenate to collecting assembly, cell suspension or homogenate flow into through collecting assembly and divide liquid subassembly, flow out by a plurality of play liquid holes that divide liquid subassembly again, from this with cell suspension or homogenate reposition of redundant personnel, reduce velocity of flow and flow, avoid a large amount of liquid to fall disturbance separation medium, influence the separation effect, cell suspension or homogenate are divided into little strand liquid post through going out the liquid hole and flow out, cell suspension or homogenate flow direction tilt up setting in every axis edge that goes out the liquid hole divide liquid subassembly, make the liquid post that flows fall on the pipe wall of centrifuging tube, the pipe wall provides frictional force and slows down liquid falling speed.
Description
Technical Field
The utility model relates to a cell separation technical field especially relates to a knockout and centrifugation divide liquid device.
Background
Density gradient centrifugation is a commonly used liquid separation method, and the principle of the method is to slowly pour cell suspension or homogenate containing various components onto the upper layer of a separation medium, and to separate the cells by the action of gravity or centrifugal force field, so as to achieve the purpose of separating the components.
The technical operation difficulty lies in that: the speed of pouring the cell suspension or homogenate over the upper layer of the separation medium is not easy to control, and too fast or too much can affect the separation effect. Generally, a pipette is needed to be used manually to suck the mixed liquid, and then the liquid is slowly released above the separation medium, so that the requirements on the stability of manual operation and the experience of an operator are higher, one person can only operate one tube of liquid at a time, the time consumption is longer, the labor consumption is higher, the cell suspension or homogenate added firstly begins to settle, and the settling effect is different after the cell suspension or homogenate added later is centrifuged.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The to-be-solved technical problem of the utility model is that the speed is difficult to the accuse when cell suspension or homogenate are poured to the separation medium upper strata in current density gradient centrifugation, the not good problem of liquid interface layering effect.
(II) technical scheme
In order to solve the technical problem, the utility model provides a liquid separator, including collecting the subassembly and dividing the liquid subassembly, the liquid outlet of collecting the subassembly with divide the inlet of liquid subassembly to connect, divide the bottom of liquid subassembly to have a plurality of liquid holes, the axis in liquid hole is followed divide the interior liquid flow direction tilt up of liquid subassembly.
Wherein, divide the inlet of liquid subassembly to be located divide the top of liquid subassembly, divide liquid subassembly inside to have from the inlet to go out the liquid flow channel that liquid hole leaned out gradually, flow channel one end with divide the inlet intercommunication of liquid subassembly, the other end with go out liquid hole intercommunication.
Wherein, divide liquid subassembly to include first round platform shape part and second round platform shape part, second round platform shape part is located the inboard of first round platform shape part, the bottom of first round platform shape part has downwardly extending's annular edge, just the bottom of second round platform shape part with the edge connection, the medial surface of first round platform shape part with form between the lateral surface of second round platform shape part fluid flow channel, the top of first round platform shape part is opened, the top of second round platform shape part is sealed, in order to form divide liquid subassembly's inlet, go out the liquid hole set up in on the edge.
Wherein, a plurality of play liquid hole is in the edge upper week evenly distributed.
The axis of the liquid outlet hole is perpendicular to the axis of the liquid distribution assembly.
Wherein, the collection assembly is funnel-shaped, the upper end is an open liquid inlet, and the lower end is a tubular liquid outlet.
The utility model provides a centrifugation divides liquid device, including centrifuging tube and as above knockout, the knockout is followed the axial of centrifuging tube inserts in the centrifuging tube, so that divide the liquid subassembly to be located in the centrifuging tube.
Wherein, the position of the bottom end of the liquid separation component is higher than the graduation line of the total volume of the separation medium and the cell suspension or homogenate on the centrifuge tube.
Wherein, the diameter of the liquid inlet of the collecting assembly is larger than the pipe diameter of the centrifugal pipe.
(III) advantageous effects
The above technical scheme of the utility model has following advantage: the utility model discloses a knockout comprises collection subassembly and branch liquid subassembly, the collection subassembly is used for the collection of mixed liquid, can once only pour cell suspension or homogenate such as dilution blood to the collection subassembly in, the inlet of branch liquid subassembly is connected with the liquid outlet of collection subassembly, cell suspension or homogenate flows into branch liquid subassembly through collection subassembly, again flow out by a plurality of liquid outlet holes of branch liquid subassembly, the axis of liquid outlet hole than liquid stream in the branch liquid subassembly according to the outflow direction of original flow direction certain angle that upwards slopes, so that the liquid column that flows out in the liquid outlet hole falls in the position of centrifuging tube pipe wall, the centrifuging tube pipe wall position that falls in than the flow direction liquid column originally is higher, increase the flow path length of liquid stream on the centrifuging tube pipe wall, divide cell suspension or homogenate from this, reduce velocity of flow and flow, avoid a large amount of liquid to fall the disturbance separation medium, influence the separation effect, cell suspension or homogenate is divided into little share liquid column through the liquid outlet hole and, the axis of each liquid outlet hole is arranged in an upward inclined mode along the flowing direction of cell suspension or homogenate in the liquid separating assembly, so that liquid flow is prevented from directly falling into the centrifugal tube, an outflow liquid column falls on the tube wall of the centrifugal tube, and the tube wall provides friction force to slow down the falling speed of liquid.
The utility model discloses can effectively improve density gradient centrifugation efficiency, especially the separation of a plurality of samples, reduce manual operation time, can be nearly simultaneously liquid feeding, make a plurality of sample layering effects keep unanimous, save cost of labor and training operating cost, only need to empty liquid in the collection subassembly and just can accomplish with the automatic layering of separation medium in the centrifuging tube, the layering interface is clear and smooth, the effect is more stable than manual operation, can hardly lead to disturbing the separation medium in the centrifuging tube because of the hand mistake, make the final poor condition of separation effect; the design structure is simple, the plastic material which is light and cheap can be used for manufacturing, and the carrying and the transportation are convenient.
In addition to the technical problems addressed by the present invention, the technical features of the constituent technical solutions, and the advantages brought by the technical features of these technical solutions, which are described above, other technical features of the present invention and the advantages brought by these technical features will be further explained with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic structural view of a liquid distributor according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a liquid separating assembly of the liquid separator according to the embodiment of the present invention;
fig. 3 is a schematic structural diagram of a centrifugal liquid separating device according to an embodiment of the present invention.
In the figure: 1: a collection assembly; 2: a liquid separating component; 3: centrifuging the tube; 21: a liquid outlet hole; 22: a first truncated cone shaped member; 23: a second truncated cone shaped member; 24: a flow channel; 221: an edge.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the description of the present invention, unless otherwise specified, "plurality", and "plural" mean two or more, and "several", and "several groups" mean one or more.
As shown in fig. 1, fig. 2 and fig. 3, the embodiment of the utility model provides a liquid separator, including collecting subassembly 1 and dividing liquid subassembly 2, the liquid outlet of collecting subassembly 1 is connected with the inlet that divides liquid subassembly 2, divides the bottom of liquid subassembly 2 to have a plurality of liquid holes 21, goes out the axis of liquid hole 21 along dividing liquid subassembly 2 interior liquid flow direction tilt up.
The utility model discloses a knockout comprises collection subassembly and branch liquid subassembly, the collection subassembly is used for the collection of mixed liquid, can once only pour cell suspension or homogenate such as dilution blood to the collection subassembly in, the inlet of branch liquid subassembly is connected with the liquid outlet of collection subassembly, cell suspension or homogenate flows into branch liquid subassembly through collection subassembly, again flow out by a plurality of liquid outlet holes of branch liquid subassembly, the axis of liquid outlet hole than liquid stream in the branch liquid subassembly according to the outflow direction of original flow direction certain angle that upwards slopes, so that the liquid column that flows out in the liquid outlet hole falls in the position of centrifuging tube pipe wall, the centrifuging tube pipe wall position that falls in than the flow direction liquid column originally is higher, increase the flow path length of liquid stream on the centrifuging tube pipe wall, divide cell suspension or homogenate from this, reduce velocity of flow and flow, avoid a large amount of liquid to fall the disturbance separation medium, influence the separation effect, cell suspension or homogenate is divided into little share liquid column through the liquid outlet hole and, the axis of each liquid outlet hole is arranged in an upward inclined mode along the flowing direction of cell suspension or homogenate in the liquid separating assembly, so that liquid flow is prevented from directly falling into the centrifugal tube, an outflow liquid column falls on the tube wall of the centrifugal tube, and the tube wall provides friction force to slow down the falling speed of liquid.
The utility model discloses can effectively improve density gradient centrifugation efficiency, especially the separation of a plurality of samples, reduce manual operation time, can be nearly simultaneously liquid feeding, make a plurality of sample layering effects keep unanimous, save cost of labor and training operating cost, only need to empty liquid in the collection subassembly and just can accomplish with the automatic layering of separation medium in the centrifuging tube, the layering interface is clear and smooth, the effect is more stable than manual operation, can hardly lead to disturbing the separation medium in the centrifuging tube because of the hand mistake, make the final poor condition of separation effect; the design structure is simple, the plastic material which is light and cheap can be used for manufacturing, and the carrying and the transportation are convenient.
Wherein, divide the inlet of liquid subassembly 2 to be located the top of dividing liquid subassembly 2, divide liquid subassembly 2 inside to have from the inlet to go out liquid hole and lean out liquid flow channel 24 gradually, liquid flow channel 24 one end and the inlet intercommunication that divides liquid subassembly 2, liquid flow channel 24 other end and play liquid hole 21 intercommunication. The liquid flow channel is obliquely arranged outwards from the inlet of the liquid separation component to the liquid outlet hole, and the flow velocity of the cell suspension or homogenate is slowed down when the cell suspension or homogenate flows through the liquid flow channel, so that the cell suspension or homogenate is primarily buffered before contacting with the upper interface of the separation medium.
The liquid separating assembly 2 comprises a first truncated cone-shaped component 22 and a second truncated cone-shaped component 23, the second truncated cone-shaped component 23 is located on the inner side of the first truncated cone-shaped component 22, the bottom end of the first truncated cone-shaped component 22 is provided with an annular edge 221 extending downwards, the bottom end of the second truncated cone-shaped component 23 is connected with the edge 221, a liquid flow channel 24 is formed between the inner side face of the first truncated cone-shaped component 22 and the outer side face of the second truncated cone-shaped component 23, the top end of the first truncated cone-shaped component 22 is open, the top end of the second truncated cone-shaped component 23 is closed to form a liquid inlet of the liquid separating assembly 2, and the liquid outlet 21 is arranged on the edge 221. Adopt the liquid separating assembly of round platform shape in this embodiment, first round platform shape part is established with second round platform shape part cover, is flow channel between first round platform shape part and the second round platform shape part, therefore flow channel is the annular seam face, has increased the flow region area of cell suspension or homogenate, has prolonged the flow time, also improves the stability of liquid stream when slowing down the velocity of flow. The bottom of first round platform shape part has the edge, and the edge is parallel state along axial downwardly extending, with the centrifuging tube pipe wall, goes out the liquid hole and sets up on the edge, by the direct injection of the liquid column that flows out in going out the liquid hole on the pipe wall, realizes through the pipe wall buffering that slows down once more.
Wherein, a plurality of liquid outlet holes 21 are evenly distributed on the edge 221 in the circumferential direction. The liquid outlet holes uniformly distributed on the edge can uniformly distribute the cell suspension or homogenate, ensure that the cell suspension or homogenate is uniformly contacted with a separation medium in a centrifugal tube, and effectively improve the layering effect.
Wherein, the axis of the liquid outlet hole 21 is vertical to the axis of the liquid separating component 2. Cell suspension or homogenate are divided into the outflow of little thigh liquid column through going out the liquid hole, and the axis in every play liquid hole is perpendicular with the pipe wall of its corresponding centrifuging tube in this embodiment, makes the liquid column that flows directly drop on the pipe wall, and the pipe wall provides frictional force and slows down liquid falling speed, goes out liquid hole axis simultaneously and divides the axis of subassembly perpendicular setting with round platform shape, and maximum stable cell suspension or homogenate are sprayed and are flowed.
Wherein, the collection component 1 is funnel-shaped, the upper end is an open liquid inlet, and the lower end is a tubular liquid outlet. Collect the subassembly in this embodiment and adopt to leak hopper-shaped, the inlet is the uncovered of upper end, and the liquid outlet is the mouth of pipe of lower extreme, and uncovered internal diameter reduces gradually and converges in mouth of pipe department, and the liquid subassembly is divided in the connection of mouth of pipe lower extreme, collects the collection that the subassembly is used for mixed liquid, can once only empty cell suspensions such as dilution blood or homogenate. The funnel has simple structure, easy material taking and low cost.
The embodiment of the utility model provides a centrifugation liquid device that divides is still provided, including centrifuging tube 3 and as above the knockout, the knockout inserts in centrifuging tube 3 along centrifuging tube 3's axial to make and divide liquid subassembly 2 to be located centrifuging tube 3.
The utility model discloses a centrifugation divides liquid device, it forms to insert the knockout in the centrifuging tube, the bottom diameter of the branch liquid subassembly of knockout slightly is less than the pipe diameter of centrifuging tube, the collection subassembly is used for the collection of mixed liquid, can once only pour cell suspensions such as dilution blood or homogenate to the collection subassembly in, the inlet of dividing the liquid subassembly is connected with the liquid outlet of collection subassembly, cell suspension or homogenate flows into dividing the liquid subassembly through the collection subassembly, flow out by a plurality of liquid outlet holes of dividing the liquid subassembly again, the axis of liquid outlet hole than divide the interior liquid stream of liquid subassembly according to the certain angle of flow direction tilt up of original flow direction, so that the liquid column that flows out in the liquid outlet hole falls on the position of centrifuging tube pipe wall, it is higher than the centrifuging tube pipe wall position that the liquid column that originally flows to fall on, increase the flow path length of liquid stream on the centrifuging tube pipe wall, from this with cell, avoid a large amount of liquid to fall the disturbance separation medium, influence the separation effect, cell suspension or homogenate are divided into little strand liquid column through going out the liquid hole and flow, and the axis in every play liquid hole is perpendicular with the pipe wall of its centrifuging tube that corresponds, makes the liquid column of effluvium directly drop on the pipe wall, and the pipe wall provides frictional force and slows down liquid falling speed.
The utility model discloses can effectively improve density gradient centrifugation efficiency, especially the separation of a plurality of samples, reduce manual operation time, can be nearly simultaneously liquid feeding, make a plurality of samples layering effect keep unanimous, save cost of labor and training operating cost, only need empty liquid can accomplish with the centrifuging tube in the separation medium's automatic layering, the layering interface is clear and level, the effect is more stable than manual operation, hardly can lead to disturbing the separation medium in the centrifuging tube because of the hand mistake, make the final separation poor condition of effect; the design structure is simple, the plastic material which is light and cheap can be used for manufacturing, and the carrying and the transportation are convenient.
Wherein, the position of the bottom end of the liquid separating component 2 is higher than the graduation line of the total volume of the separation medium and the cell suspension or homogenate on the centrifuge tube 3. The size of the liquid separator can be adjusted according to centrifugal tubes with different tube diameters, the overall height of the liquid separator is not too long, and the bottom end of the liquid separating component is preferably higher than the scale mark of the total volume of the separation medium and the cell suspension or homogenate.
Wherein, the diameter of the liquid inlet of the collecting assembly 1 is larger than the diameter of the centrifugal tube 3. Funnel-shaped collecting assembly's inlet diameter is greater than the pipe diameter of centrifuging tube, can make the inlet part of funnel be located the outside of centrifuging tube when the knockout uses, avoids the hand mistake to put into the centrifuging tube with mixed liquid in, guarantees that mixed liquid is whole to get into in the funnel.
To sum up, the utility model discloses a knockout comprises collection subassembly and branch liquid subassembly, the collection subassembly is used for the collection of mixed liquid, can once only pour cell suspensions such as dilution blood or homogenate to the collection subassembly, the inlet of dividing the liquid subassembly is connected with the liquid outlet of collection subassembly, cell suspension or homogenate flow into through the collection subassembly divides the liquid subassembly, again by a plurality of liquid outlet holes of dividing the liquid subassembly outflow, the axis of liquid outlet hole than divide the interior liquid stream of liquid subassembly according to the certain angle of flow direction tilt up of original flow direction, so that the liquid column that flows out in the liquid outlet hole falls in the position of centrifuging tube pipe wall, the centrifuging tube pipe wall position that falls in the liquid column that flows to originally is higher, increase the flow path length of liquid stream on the centrifuging tube pipe wall, divide cell suspension or homogenate from this, reduce velocity of flow and flow, avoid a large amount of liquid to fall the disturbance separation medium, influence the separation effect, cell suspension or homogenate are divided into the outflow of strand liquid column through going out the liquid hole, and the axis in every play liquid hole sets up along dividing the interior cell suspension of liquid subassembly or homogenate flow direction tilt up, avoids the direct whereabouts of liquid stream to the centrifuging tube in, makes the liquid column that flows fall on the pipe wall of centrifuging tube, and the pipe wall provides frictional force and slows down liquid falling speed.
The utility model discloses can effectively improve density gradient centrifugation efficiency, especially the separation of a plurality of samples, reduce manual operation time, can be nearly simultaneously liquid feeding, make a plurality of samples layering effect keep unanimous, save cost of labor and training operating cost, only need empty liquid can accomplish with the centrifuging tube in the separation medium's automatic layering, the layering interface is clear and level, the effect is more stable than manual operation, hardly can lead to disturbing the separation medium in the centrifuging tube because of the hand mistake, make the final separation poor condition of effect; the design structure is simple, the plastic material which is light and cheap can be used for manufacturing, and the carrying and the transportation are convenient.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (9)
1. A dispenser, its characterized in that: including collecting the subassembly with divide liquid subassembly, the liquid outlet of collecting the subassembly with divide liquid subassembly's inlet to be connected, divide liquid subassembly's bottom to have a plurality of liquid holes, the axis in liquid hole is followed divide liquid subassembly interior liquid flow to tilt up.
2. The dispenser according to claim 1, wherein: divide the inlet of liquid subassembly to be located divide the top of liquid subassembly, divide liquid subassembly inside to have from the inlet to go out the liquid flow channel that the liquid hole leaned out gradually, liquid flow channel one end with divide the inlet intercommunication of liquid subassembly, the other end with go out liquid hole intercommunication.
3. The dispenser according to claim 2, characterized in that: divide liquid subassembly to include first round platform shape part and second round platform shape part, second round platform shape part is located the inboard of first round platform shape part, the bottom of first round platform shape part has downwardly extending's annular edge, just the bottom of second round platform shape part with the edge connection, the medial surface of first round platform shape part with form between the lateral surface of second round platform shape part fluid flow channel, the top of first round platform shape part is opened, the top of second round platform shape part is sealed, in order to form divide liquid subassembly's inlet, go out the liquid hole set up in on the edge.
4. The dispenser according to claim 3, wherein: the liquid outlet holes are circumferentially and uniformly distributed on the edge.
5. The dispenser according to claim 4, wherein: the axis of the liquid outlet hole is vertical to the axis of the liquid distribution assembly.
6. The dispenser according to claim 1, wherein: the collecting assembly is funnel-shaped, the upper end of the collecting assembly is an open liquid inlet, and the lower end of the collecting assembly is a tubular liquid outlet.
7. The utility model provides a centrifugation divides liquid device which characterized in that: comprising a centrifuge tube and a dispenser according to any of claims 1 to 6, which dispenser is inserted into the centrifuge tube in the axial direction thereof, so that the dispensing assembly is located in the centrifuge tube.
8. The centrifugal liquid separating device according to claim 7, wherein: the bottom end of the liquid separation component is higher than the scale mark of the total volume of the separation medium and the cell suspension or homogenate on the centrifugal tube.
9. The centrifugal liquid separating device according to claim 7, wherein: the diameter of the liquid inlet of the collecting assembly is larger than the pipe diameter of the centrifugal pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920182725.5U CN209923312U (en) | 2019-02-01 | 2019-02-01 | Liquid separator and centrifugal liquid separating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920182725.5U CN209923312U (en) | 2019-02-01 | 2019-02-01 | Liquid separator and centrifugal liquid separating device |
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| Publication Number | Publication Date |
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| CN209923312U true CN209923312U (en) | 2020-01-10 |
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| CN201920182725.5U Active CN209923312U (en) | 2019-02-01 | 2019-02-01 | Liquid separator and centrifugal liquid separating device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113617065A (en) * | 2021-07-29 | 2021-11-09 | 张华� | Liquid-gas separator for petroleum exploitation and liquid-gas separation method thereof |
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2019
- 2019-02-01 CN CN201920182725.5U patent/CN209923312U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113617065A (en) * | 2021-07-29 | 2021-11-09 | 张华� | Liquid-gas separator for petroleum exploitation and liquid-gas separation method thereof |
| CN113617065B (en) * | 2021-07-29 | 2023-09-15 | 重庆华川油建装备制造(集团)股份有限公司 | Petroleum exploitation liquid-gas separator and liquid-gas separation method thereof |
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