CN209894577U

CN209894577U - Sampling tool for observing prepared root epidermal cells

Info

Publication number: CN209894577U
Application number: CN201920512007.XU
Authority: CN
Inventors: 刘胜群; 宋凤斌; 李向楠
Original assignee: Northeast Institute of Geography and Agroecology of CAS
Current assignee: Northeast Institute of Geography and Agroecology of CAS
Priority date: 2019-04-15
Filing date: 2019-04-15
Publication date: 2020-01-03
Anticipated expiration: 2029-04-15

Abstract

A sampling tool for observing the root epidermal cells is prepared. The utility model particularly relates to a sampling tool for preparing root epidermal cell observation. The utility model discloses a solve the root footpath and be less than 1 cm's plant organ epidermis and hardly adopt the method of tearing to peel off to take a sample the problem. The hairbrush comprises a fixing needle, a base, a hairbrush, a chute body, a handle, a hairbrush rod, a fixing screw and a linear slideway; the base is a disc, the fixed needle vertical fixation is put at the central point of base, it has sharp slide to open in the middle of the spout body, the side of sharp slide is provided with the set screw. The utility model is suitable for a root system epidermal cell's that the root system diameter is greater than 3mm sample observation.

Description

Sampling tool for observing prepared root epidermal cells

Technical Field

The utility model particularly relates to a sampling tool for preparing root epidermal cell observation.

Background

The root system is an important organ of the plant, and plays important roles of fixing and supporting, absorbing water and nutrients, synthesizing bioactive substances and conveying the bioactive substances to the stems and leaves of the overground part in the higher plant and the like. With the enhancement of the recognition of the root system function by people, the research of the root system is also increasingly concerned by researchers. There is a close relationship between the structure of the root system and the function of the root system. Therefore, the research on the root system structure is also regarded as important.

The root system is cylindrical, and the surface of the root system is a curved surface and not a plane, so that the sampling work is difficult, and the cross section of the root system is observed mostly aiming at the observation of the root system structure. But the observation of the cross-section only allows one or a few layers of cells to be observed at the sampling site of the cross-section. Since the root system is in the form of a slender cylinder, observation of the microstructure in a cross section is not satisfactory if the properties and arrangement of epidermal cells in the longitudinal direction are further observed. Therefore, new tools and methods are needed to meet the observation of root axial epidermal cells.

One of the existing observation methods for plant organ epidermis is a method for tearing and peeling off the epidermis, but the method is not suitable for observing the epidermal cells of the root system because the epidermis of the root system is not easy to tear and peel; the second method is a nail polish smearing method on the surface of the leaf, and the conventional operation method for smearing the nail polish is that a test material is held by one hand, a nail polish brush is held by the other hand, the test material is brushed on the test material, then the test material can be put down until the nail polish on the test material is solidified, and after the nail polish is dried in the air, the nail polish is taken down from the leaf and is put on a glass slide for the microstructure officer to produce. However, the method mainly aims at the relatively flat blade, the root system is in a cylindrical shape, the epidermis forms a curved surface and is not as plane as the root system, the epidermal cells of the root system are difficult to take samples by adopting a tearing and stripping method, and meanwhile, if the diameter of the root is relatively small (less than 1cm), the nail polish is difficult to take off and observe by using a method of smearing the nail polish.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problem that the epidermis of a plant organ with the root diameter smaller than 1cm is difficult to take samples by adopting a tearing and stripping method, and provides a sampling tool for observing the epidermis cells of a root.

The utility model relates to a sampling tool for observing root epidermal cells, which comprises a fixed needle, a base, a brush, a chute body, a handle, a brush rod, a fixed screw and a linear slideway; the base is a disc, the fixing needle is vertically fixed at the central position of the base, a linear slideway is formed in the middle of the slideway, and a fixing screw is arranged on the side surface of the linear slideway; one end of the chute body is fixedly connected with a handle, the other end of the chute body is fixedly connected with a circular ring, and the circular ring is sleeved on the fixing needle; the brush rod is perpendicular to the base, the lower end of the brush rod is arranged in the linear slideway in a sliding mode, the brush rod is locked through a fixing screw, and a row of brushes are arranged on the outer surface of the brush rod in the axial direction.

The utility model has the advantages that:

the utility model discloses an apparatus and method of use are applicable to the observation that root system diameter is greater than 3 mm's root system epidermic cell, and the nail polish that will paint that can be complete takes off the observation. The utility model discloses it is fixed earlier with the trial material of root system, then wave with the help of the handle and drive the nail polish brush, paint the back, the nail polish brush keeps away from the trial material of root system with the help of the track, and the overall process need not hold the trial material with the hand, and the nail polish that need not wait for on the trial material simultaneously solidifies. Moreover, the handle is shaken to drive the nail polish brush to move, so that the polish brush can be well controlled. The distance between the oil brush and the surface of the test material is constant, so that the phenomenon that the distance between the nail oil brush and the test material is different due to different force strength of manual operation during manual operation, the thickness of the nail oil paint is not uniform, and the next observation is influenced due to the fact that the nail oil paint is not uniform is avoided. Therefore, the utility model has the advantages that firstly, it is fixed with the root system examination material, avoid always needing to hand the examination material in operation process. Secondly, the nail polish can be ensured to be evenly smeared on the test material. The phenomenon of uneven thickness of the nail polish manually operated is avoided.

Drawings

FIG. 1 is a schematic view showing the construction of a sampling tool for root epidermal cell observation;

fig. 2 is a schematic top view of the track device.

Detailed Description

The technical solution of the present invention is not limited to the following embodiments, and includes any combination of the following embodiments, and the following embodiments are described with reference to fig. 1 and 2.

The first embodiment is as follows: the sampling tool for preparing root epidermal cell observation in the embodiment comprises a fixing needle 1, a base 2, a brush 3, a chute body 4, a handle 5, a brush rod 6, a fixing screw 7 and a linear slideway 8; the base 2 is a disc, the fixing needle 1 is vertically fixed at the central position of the base 2, a linear slideway 8 is arranged in the middle of the slideway 4, and a fixing screw 7 is arranged on the side surface of the linear slideway 8; one end of the chute body 4 is fixedly connected with a handle 5, the other end of the chute body 4 is fixedly connected with a circular ring, and the circular ring is sleeved on the fixing needle 1; the brush rod 6 is perpendicular to the base 2, the lower end of the brush rod 6 is arranged in the linear slideway 8 in a sliding mode, the brush rod 6 is locked through the fixing screw 7, and the outer surface of the brush rod 6 is provided with a row of brushes 3 along the axial direction.

The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: the diameter of the base 2 is 8 cm. The rest is the same as the first embodiment.

The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the length of the fixing needle 1 is 5cm, and the diameter of the fixing needle is 0.5-1.0 mm. The others are the same as in the first or second embodiment.

The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: the length of the brush rod 6 is 5cm, and the length of the brush 3 is 0.3-1.0 cm. The rest is the same as one of the first to third embodiments.

The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is: the length of the linear slideway 8 is 4 cm. The rest is the same as one of the first to fourth embodiments.

The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is: the sampling tool for preparing the root epidermal cell observation further comprises an annular slide way 9, the annular slide way 9 is concentrically arranged on the circumferential surface of the base 2, and a slide block matched with the annular slide way 9 is arranged on the bottom surface of the outer side of the slide groove body 4. The rest is the same as one of the first to fifth embodiments.

The seventh embodiment: the difference between this embodiment and one of the first to sixth embodiments is: the inner diameter of the annular slide way 9 is 3 mm. The rest is the same as one of the first to sixth embodiments.

The use method of the sampling tool for preparing root epidermal cell observation is carried out according to the following steps:

firstly, treating a root system to be observed: washing the corn root system to be observed with water, washing the soil attached to the corn root system, and then sucking water on the surface of the root system with filter paper; removing lateral roots on the root system by using a scalpel;

secondly, fixing root system materials: the center of a cross section of the root section to be measured is aligned with the needle point of the fixed needle 1 and penetrates along the axial direction, so that the fixed needle 1 is positioned at the axis position of the root system;

thirdly, preparing the brush: dipping the brush 3 with transparent nail polish, uniformly distributing the transparent nail polish on the brush, then pushing the brush rod 6 to slide on the chute body 4, and screwing the fixing screw 7 to fix the brush rod 6 on the chute body 4 when the brush 3 is fully contacted with the surface of the corn root system;

fourthly, smearing nail polish: rotating the handle 5 to enable the brush 3 to do uniform circular motion by taking the fixed needle 1 as a center, and uniformly smearing the nail polish on the brush 3 on the surface of the corn root system; according to the size observed by the experiment requirement, the control handle 5 enables the brush 3 to stop at a proper position, then the fixing screw 7 is unscrewed, and the brush rod 6 moves towards the outer side of the rail device 4, so that the brush rod 6 is far away from the fixing needle 1;

fifthly, taking down the nail polish: after the nail polish was sufficiently dried, it was removed with forceps and placed on a glass slide for further observation.

Claims

1. A sampling tool for preparing root epidermal cell observation is characterized by comprising a fixing needle (1), a base (2), a brush (3), a chute body (4), a handle (5), a brush rod (6), a fixing screw (7) and a linear slideway (8); the base (2) is a disc, the fixing needle (1) is vertically fixed at the central position of the base (2), a linear slideway (8) is arranged in the middle of the slideway body (4), and a fixing screw (7) is arranged on the side surface of the linear slideway (8); one end of the chute body (4) is fixedly connected with a handle (5), the other end of the chute body (4) is fixedly connected with a circular ring, and the circular ring is sleeved on the fixing needle (1); the brush rod (6) is perpendicular to the base (2) and is arranged, the lower end of the brush rod (6) is arranged in the linear slide way (8) in a sliding mode, the brush rod (6) is locked through the fixing screws (7), and the outer surface of the brush rod (6) is provided with a row of brushes (3) along the axial direction.

2. The sampling tool for preparing root epidermal cell observation according to claim 1, wherein said base (2) has a diameter of 8 cm.

3. The sampling tool for observing the prepared root epidermal cells in claim 2, wherein the length of the fixing needle (1) is 5cm, and the diameter is 0.5-1.0 mm.

4. The sampling tool for observing the prepared root epidermal cells of claim 1, wherein the length of said brush rod (6) is 5cm, and the length of said brush (3) is 0.3-1.0 cm.

5. The sampling tool for preparing root epidermal cell observation according to claim 1, wherein said linear slide (8) has a length of 4 cm.

6. The sampling tool for observing the prepared root epidermal cells is characterized by further comprising an annular slide way (9), the annular slide way (9) is concentrically arranged on the circumferential surface of the base (2), and the bottom surface of the outer side of the slide groove body (4) is provided with a slide block matched with the annular slide way (9).

7. A sampling tool for preparing root epidermal cell observation according to claim 6, characterized in that the inner diameter of said annular slide way (9) is 3 mm.