CN209872943U - Device for filtering chondrocytes - Google Patents

Abstract

The utility model provides a device for filtering chondrocytes, the utility model relates to the technical field of cell culture equipment, the upper end of a bottom cap is provided with a rotary connection frame, a plurality of skeletons are fixed on the rotary connection frame in a circular rotary connection manner, a fixed rod is inserted and fixed in the middle of the rotary connection frame, a loop bar is movably sleeved on the fixed rod, the lower end of the loop bar is provided with a rotary connection frame II, a plurality of supporting frames corresponding to the skeletons are fixed on the rotary connection frame II in a rotary connection manner, the other ends of the supporting frames are fixed in the middle of the corresponding skeletons in a hinged manner, and an elastic wrapping surface is wrapped and fixed on the outer side of the whole body; the outer side surface of the elastic wrapping surface is wrapped with filter cloth, an iron fixing block is movably sleeved on the loop bar, the lower surface of the fixing block is abutted against the upper end surface of the framework, a plurality of magnets are fixedly adsorbed on the outer side wall of the fixing block, the upper edge of the filter cloth is adsorbed by the magnets and is wrapped and fixed on the fixing block, and the upper end of the outer side wall of the fixing block is fixedly connected with a rubber band; the fixing device can be easily removed without damaging the filter cloth.

Description

Device for filtering chondrocytes

Technical Field

The utility model relates to a cell culture equipment technical field, concretely relates to device is used in chondrocyte filtration.

Background

In the primary culture of chondrocytes, the cartilage to be removed needs to be minced and then digested with two enzymes for a plurality of times to obtain chondrocytes, so that the cartilage to be digested and an enzyme solution containing chondrocytes are filtered into a centrifugal tube through gauze for a plurality of times to obtain a solution containing only chondrocytes; in the past, two pairs of tweezers are used for folding gauze into a triangular block so as to form a thin funnel structure, then the folded gauze funnel is quickly inserted into a centrifugal tube, enzyme solution dissolved with cartilage tissues and cartilage cells is sucked up by a gun, and is quickly inserted into the tip of an inner funnel on the outermost layer of the gauze funnel and simultaneously props against the wall of the centrifugal tube so as to be slowly filtered; therefore, the pollution probability is easily increased, the filtering efficiency is not high, and the filtering cloth is unstable and easy to slide; use fixing device among the prior art to fix the filter cloth for the funnel shape to fix at the examination mouth of pipe with the rubber band, nevertheless when extracting fixing device, fixing device and filter cloth are difficult to avoid producing the friction, drive the filter cloth and remove, destroy the shape of filter cloth, above problem is awaited the improvement urgently.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a simple structure, reasonable in design, convenient to use's device for chondrocyte filtration to prior art's defect and not enough can be under the condition of not destroying the filter cloth light fixing device that gets rid of.

In order to achieve the above object, the utility model adopts the following technical scheme: the filter cloth comprises a fixing device and filter cloth, wherein the fixing device comprises a bottom cap, a fixing rod, a framework, a supporting frame, a first screwing frame, a second screwing frame, a fixing block and an elastic covering surface, the upper end of the bottom cap is provided with the first screwing frame, a plurality of frameworks are fixedly screwed on the first screwing frame in a ring-shaped manner, the fixing rod is fixedly inserted in the middle of the first screwing frame, a sleeve rod is movably sleeved on the fixing rod, the lower end of the sleeve rod is provided with the second screwing frame, a plurality of supporting frames corresponding to the frameworks are fixedly screwed on the second screwing frame, the other ends of the supporting frames are fixedly hinged in the middle of the corresponding frameworks, and the elastic covering surface is fixedly covered on the outer side of the whole body formed by the bottom cap and the frameworks; the outside bread of elasticity package face is equipped with the filter cloth, and the movable sleeve is equipped with the iron fixed block on the loop bar, and the lower surface of fixed block is contradicted with the up end of skeleton and is set up, adsorbs on the lateral wall of fixed block to be fixed with the several magnetite, and the top edge of filter cloth is adsorbed and is established through the magnetite and wrap and fix on the fixed block, the upper end fixedly connected with rubber band of the lateral wall of fixed block.

Furthermore, the upper side of the loop bar is fixedly connected with a loose screw, and the loose screw penetrates through the loop bar and then is abutted against the outer side wall of the fixed rod.

Furthermore, the elastic covering is latex elastic covering.

Furthermore, the framework and the support frame are both steel structures.

Furthermore, the filter cloth is a filter cloth for the Nissan order.

After the structure is adopted, the utility model discloses beneficial effect does: a chondrocyte filters and uses device, its fixing device has scalability, when fixing the filter cloth at the mouth of pipe of trying on, under the condition that does not destroy the filter cloth shape, can easily take out fixing device, the utility model has the advantages of simple structure, it is reasonable to set up, the cost of manufacture is low.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the test tube of the present invention.

Fig. 3 is a schematic structural view of the present invention taken out from the test tube.

Description of reference numerals:

the device comprises a fixing device 1, filter cloth 2, a bottom cap 3, a fixing rod 4, a framework 5, a supporting frame 6, a first screwing frame 7, a second screwing frame 8, a fixing block 9, an elastic cladding 10, a loop bar 11, a magnet 12, a rubber band 13 and an elastic screw 14.

Detailed Description

The present invention will be further described with reference to the accompanying drawings.

Referring to fig. 1 to fig. 3, the technical solution adopted by the present embodiment is: the cloth filter comprises a fixing device 1 and filter cloth 2, wherein the fixing device 1 comprises a bottom cap 3, a fixing rod 4, a framework 5, a support frame 6, a first screwing frame 7, a second screwing frame 8, a fixing block 9 and an elastic covering surface 10, the upper end of the bottom cap 3 is integrally formed with the first screwing frame 7, the first screwing frame 7 is annularly screwed and fixed with a plurality of frameworks 5 by hinges, the fixing rod 4 is inserted and welded in the middle of the first screwing frame 7, a sleeve rod 11 is movably sleeved on the fixing rod 4, a loose screw 14 is fixedly connected to the upper side of the sleeve rod 11, and the loose screw 14 is abutted against the outer side wall of the fixing rod 14 after penetrating through the sleeve rod 11; the lower end of the loop bar 11 is integrally formed with a second rotary connection frame 8 in an injection molding mode, a plurality of support frames 6 corresponding to the framework 5 are fixedly connected to the second rotary connection frame 8 in a rotary mode through hinges, and the framework 5 and the support frames 6 are both of steel structures; the other end of the supporting frame 6 is hinged and fixed in the middle of the corresponding framework 5 by a hinge, and the outside of the whole body formed by the bottom cap 3 and the framework 5 is wrapped and is bonded and fixed with an elastic wrapping surface 10 by glue; the elastic covering 10 is latex elastic covering; the outside bread of elasticity package face 10 is equipped with filter cloth 2, and filter cloth 2 is 150 meshes filter cloth, and the movable sleeve is equipped with iron fixed block 9 on the loop bar 11, and the lower surface of fixed block 9 is contradicted with the up end of skeleton 5 and is set up, adsorbs on the lateral wall of fixed block 9 to be fixed with several magnetite 12, and the top edge of filter cloth 2 adsorbs and wraps through magnetite 12 and establishes to be fixed on fixed block 9, the upper end fixedly connected with rubber band 13 of the lateral wall of fixed block 9.

The working principle of the specific embodiment is as follows: firstly, inserting a fixing device 1 into a test tube, unscrewing a tightening screw 14, moving a loop bar 11, driving a second screwing frame 8 to move downwards by the loop bar 11 so as to drive a support frame 6 to prop open a framework 5, propping open an outer elastic covering 10 by the framework 5 until the upper end surface of the elastic covering 10 is abutted against the inner wall of the test tube, screwing a loosening nut 14 so as to fix the positions of the framework 5 and the elastic covering 10, taking out the fixing device 1, wrapping a filter cloth 2 on the fixing device 1, fixing by a magnet 12, inserting the fixing device 1 together with the filter cloth 2 into the test tube again, taking out the magnet 12, turning down the outer wall of the test tube by the filter cloth 2, sliding down a rubber band 13 on a fixing block 9, fixing the filter cloth 2 on the test tube, unscrewing the loosening screw 14 again, moving the loop bar 11 together with the second screwing frame 8 upwards, driving the framework 5 and the elastic covering 10 to contract by the support frame 6, the fixing device 1 is taken out, the filter cloth 2 is fixed in the test tube port according to the original shape of the elastic covering surface 10, the chondrocyte filtration operation can be carried out, after the operation is finished, the rubber band 13 is detached, and the filter cloth 2 is taken out for processing.

After adopting above-mentioned structure, this embodiment's beneficial effect does: this embodiment a device for filtering chondrocyte, its fixing device have scalability, when fixing the filter cloth at the mouth of pipe of examination, under the condition that does not destroy the filter cloth shape, can easily take out fixing device, the utility model has the advantages of simple structure sets up rationally, and the cost of manufacture is low.

The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent replacements made by those of ordinary skill in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solutions of the present invention.

Claims (5)

1. The utility model provides a device is used in chondrocyte filtration, it contains fixing device (1), filter cloth (2), its characterized in that: the fixing device (1) is composed of a bottom cap (3), a fixing rod (4), a framework (5), a supporting frame (6), a first rotary connecting frame (7), a second rotary connecting frame (8), a fixing block (9) and an elastic covering surface (10), the upper end of the bottom cap (3) is provided with a rotary connection frame (7), a plurality of frameworks (5) are fixedly connected on the rotary connection frame (7) in an annular rotary connection manner, a fixed rod (4) is inserted and fixed in the middle of the rotary connection frame (7), a sleeve rod (11) is movably sleeved on the fixed rod (4), a second rotary connection frame (8) is arranged at the lower end of the sleeve rod (11), a plurality of support frames (6) corresponding to the frameworks (5) are fixedly connected on the second rotary connection frame (8) in a rotary connection manner, the other ends of the support frames (6) are fixedly hinged in the middles of the corresponding frameworks (5), and an elastic wrapping surface (10) is wrapped and fixed on the outer side of the whole body formed by the bottom cap (3) and the frameworks (5); the outside bread of elasticity package face (10) is equipped with filter cloth (2), the movable sleeve is equipped with iron fixed block (9) on loop bar (11), the lower surface of fixed block (9) is contradicted with the up end of skeleton (5) and is set up, adsorb on the lateral wall of fixed block (9) and be fixed with several magnetite (12), the top edge of filter cloth (2) passes through magnetite (12) and adsorbs and the package is established and fix on fixed block (9), the upper end fixedly connected with rubber band (13) of the lateral wall of fixed block (9).

2. The chondrocyte filter according to claim 1, wherein: the upper side of the sleeve rod (11) is fixedly connected with an elastic screw (14), and the elastic screw (14) penetrates through the sleeve rod (11) and then is abutted against the outer side wall of the fixing rod (4).

3. The chondrocyte filter according to claim 1, wherein: the elastic covering (10) is latex elastic covering.

4. The chondrocyte filter according to claim 1, wherein: the framework (5) and the support frame (6) are both steel structures.

5. The chondrocyte filter according to claim 1, wherein: the filter cloth (2) is 150 days-200 meshes.