CN209858433U - Imaging device for pre-stained markers on biological sample membrane - Google Patents
Imaging device for pre-stained markers on biological sample membrane Download PDFInfo
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- CN209858433U CN209858433U CN201920416646.6U CN201920416646U CN209858433U CN 209858433 U CN209858433 U CN 209858433U CN 201920416646 U CN201920416646 U CN 201920416646U CN 209858433 U CN209858433 U CN 209858433U
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Abstract
The utility model discloses an imaging device of a pre-dyed marker on a biological sample membrane, which comprises a shell, light source equipment and a photoelectric conversion element; a darkroom space is formed inside the shell; the light source equipment and the photoelectric conversion element are arranged in the shell; the biological sample film is arranged between the light source equipment and the photoelectric conversion element, and the biological sample film is loaded with a marker of a pre-dyed molecular weight standard sample; the photoelectric conversion element is used for acquiring a corresponding optical signal at the position of the marker on the biological sample membrane and converting the optical signal into a marker image corresponding to the marker. The utility model provides a darkroom space is formed inside the shell in the imaging device, when the light source equipment is started, the photoelectric conversion element obtains the image corresponding to the marker on the biological sample membrane, thereby realizing the image acquisition of the marker on the biological sample membrane by a contact type imaging mode, and the imaging is clear; meanwhile, the imaging device has the advantages of small structure, low manufacturing cost, convenience in operation, convenience in carrying and the like.
Description
Technical Field
The utility model relates to a sample detection technical field, in particular to sign's image device of dying in advance on biological sample membrane.
Background
In biomedical research, electrophoresis, molecular sieve separation and the like of biopharmaceuticals such as proteins, nucleic acids and the like and detection of target components are routine experimental operations. In these experiments, markers of pre-stained molecular weight standards are often used to help determine the exact location of the target component.
For example, in the immunoblotting technique, prestained protein molecular weight standards can provide a molecular weight reference for a target sample. Currently, two non-contact imaging methods are mainly included in the immunoblotting technology: 1) the film method utilizes the adhesion of the film and the sample film, and the chemiluminescence sample signal is captured by the film to form an image. The film method has high imaging quality, but because the protein standard sample can not self-illuminate or chemically illuminate, the film can only capture the sample signal but can not capture the image formed by the molecular marker. In actual operation, in order to find the position of the protein molecular weight standard sample on the film, the sample film is attached to the original position on the film again after the film is developed, and then the strip of the protein molecular weight standard sample is drawn on the film by taking the sample film as a substrate, so that the problem of complicated operation procedures exists; 2) camera method, which directly captures molecular markers on a sample membrane by photographing. However, the CCD (charge coupled device) of the camera is limited by factors such as distance and sensitivity, and the loss of optical signals is large, so that a long time exposure is often required to obtain a sufficiently clear signal. In addition, the two imaging systems have the defects of large volume, large occupied space, high cost, difficulty in carrying and the like.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is that there is the operation process complicacy, consuming time longer in the imaging mode of the marker of dying in advance on the biological sample membrane among the prior art, and imaging system has defects such as bulky, occupation space is big, with high costs, difficult transport, aim at provides the imaging device of the marker of dying in advance on the biological sample membrane.
The utility model discloses an above-mentioned technical problem is solved through following technical scheme:
the utility model provides an imaging device of a pre-dyed marker on a biological sample film, which comprises a shell, light source equipment and a photoelectric conversion element;
a darkroom space is formed inside the shell;
the light source equipment and the photoelectric conversion element are arranged in the shell;
the biological sample film is arranged between the light source equipment and the photoelectric conversion element, and the biological sample film is loaded with a marker of a pre-dyed molecular weight standard sample;
wherein the light transmission of the marker is less than the light transmission of the biological sample film;
the photoelectric conversion element is used for acquiring a corresponding optical signal at the position of the marker on the biological sample film after the light source device is started, and converting the optical signal into a marker image corresponding to the marker.
Preferably, the housing comprises a light-shielding cover and a base;
one side of the shading cover is hinged with one side of the base;
when the shading cover and the base are closed, the interior of the shell forms the darkroom space.
Preferably, the biological sample film is attached to the surface of the photoelectric conversion element.
Preferably, the imaging device further comprises a protective film;
and two sides of the protective film are respectively attached to the biological sample film and the photoelectric conversion element.
Preferably, the imaging device further comprises a package;
an outer edge of the protective film is located outside an outer edge of the photoelectric conversion element;
the top of the base is provided with a containing groove, and the photoelectric conversion element and the protective film are positioned in the containing groove;
the package member acts on the protective film and the base to restrict movement of the photoelectric conversion element relative to the base.
Preferably, the outer edge of the protective film abuts against the inner wall surface of the accommodating groove; and/or the presence of a gas in the gas,
the package member includes a filler located in an area surrounded by the photoelectric conversion element, the protective film, and a surface of the base member, for fixing the photoelectric conversion element in the base member.
Preferably, the filler is gel, glass powder or plastic blank; and/or the presence of a gas in the gas,
the package comprises a clamping plate, and when the photoelectric conversion element is in a packaging state, the clamping plate abuts against the peripheral edge of the protective film.
Preferably, the base includes a bottom plate and a base main body, the base main body is provided with the accommodating groove, the accommodating groove penetrates through the base main body, the bottom plate is movably connected to the bottom of the base main body so as to open or close the accommodating groove from the bottom of the base main body, and the height of the photoelectric conversion element is not greater than the depth of the accommodating groove.
Preferably, the photoelectric conversion element includes a detector body and a substrate, an outer edge of the substrate being located outside an outer edge of the detector body.
Preferably, the substrate is a metal plate or a PCBA (printed circuit board assembly) plate; and/or the presence of a gas in the gas,
the protective film is made of a toughened glass film or a hard plastic film; and/or the presence of a gas in the gas,
the photoelectric conversion element includes a CMOS (complementary metal oxide semiconductor) chip, a CCD chip, or an amorphous silicon photoelectric conversion detector.
Preferably, the light source device is arranged at the top position of the inner side of the shading cover; and/or the presence of a gas in the gas,
the corresponding light-emitting duration is 10ms-1000ms after the light source equipment is started; and/or the presence of a gas in the gas,
the light source device comprises a plurality of LED (light emitting diode) lamp beads arranged in a lattice shape, a plurality of lamps guided by optical fibers, a plurality of lamp tubes arranged in parallel or a plurality of plate-shaped lamps.
Preferably, the imaging device further comprises a light-diffusing plate;
the light diffusion plate is fixedly arranged on the inner side of the shading cover and is positioned right below the light source equipment; and/or the presence of a gas in the gas,
the biological sample membrane comprises a protein membrane, an agarose gel block, an agarose gel strip, a polyacrylamide gel block or a polyacrylamide gel strip.
The utility model discloses an actively advance the effect and lie in:
the utility model provides an imaging device includes casing, light source equipment and photoelectric conversion component, and the darkroom space is formed to the casing inside, and after light source equipment opened, photoelectric conversion component acquireed the image that the sign corresponds on the biological sample membrane to realize the image acquisition of sign on the biological sample membrane through the mode of contact formation of image, and the formation of image is clear; meanwhile, the imaging device has the advantages of small structure, low manufacturing cost, convenience in operation, convenience in carrying and the like.
Drawings
Fig. 1 is a schematic structural view of an imaging device for pre-staining markers on a biological sample membrane according to embodiment 1 of the present invention.
Fig. 2 is a first structural diagram of a housing in an imaging apparatus for a marker prestained on a biological sample membrane according to embodiment 1 of the present invention.
Fig. 3 is a second structural diagram of a housing in an imaging apparatus for a marker prestained on a biological sample membrane according to embodiment 1 of the present invention.
Fig. 4 is a schematic diagram of a marker image in an imaging device for a marker prestained on a biological sample membrane according to example 1 of the present invention.
Fig. 5 is a schematic view of a first structure of an imaging device for pre-stained markers on a biological sample membrane according to embodiment 2 of the present invention.
Fig. 6 is a second structural diagram of an imaging device for pre-stained markers on a biological sample membrane according to embodiment 2 of the present invention.
Fig. 7 is a third structural diagram of an imaging device for pre-stained markers on a biological sample membrane according to embodiment 2 of the present invention.
Detailed Description
The present invention is further illustrated by way of the following examples, which are not intended to limit the scope of the invention.
Example 1
As shown in fig. 1, the imaging apparatus of a marker prestained on a biological sample film of the present embodiment includes a housing 1, a light source device 2, and a photoelectric conversion element 3.
The interior of the housing 1 forms a darkroom space.
Specifically, as shown in fig. 2, the housing 1 includes a light shielding cover 4 and a base 5, and one side of the light shielding cover 4 and one side of the base 5 are hinged with the light shielding cover 4 in an open state.
As shown in fig. 3, when the light-shielding cover 4 and the base 5 are closed, a darkroom space is formed inside the housing to ensure effective collection of weak signals corresponding to pre-dyed markers on the biological sample membrane.
The light source device 2 and the photoelectric conversion element 3 are both arranged in the shell 1, wherein a biological sample film A is arranged between the light source device 2 and the photoelectric conversion element 3, and the biological sample film A carries a marker of a pre-dyed molecular weight standard sample.
The biological sample film may be directly attached to the surface of the photoelectric conversion element 3.
Wherein the light transmittance of the marker is less than the light transmittance of the biological sample film;
the biological sample membrane comprises a protein membrane, an agarose gel block, an agarose adhesive tape, a polyacrylamide gel block, a polyacrylamide gel adhesive tape and the like; when the biological sample membrane is a protein membrane, the marker is a marker of a prestained protein molecular weight standard.
After the operations of placing the biological sample film a bearing the marker of the pre-dyed molecular weight standard sample on the surface of the photoelectric conversion element 3, covering the light shielding cover and starting the light source device 2 to emit light are sequentially completed, the photoelectric conversion element 3 is used for synchronously acquiring the optical signal corresponding to the position of the marker on the biological sample film a within the light emitting duration of the light source device 2 and converting the optical signal into the marker image corresponding to the marker.
The photoelectric conversion element includes a CMOS chip, a CCD chip, or an amorphous silicon photoelectric conversion detector.
The corresponding light-emitting duration after the light source equipment is started is 10ms-1000ms, and the light-emitting duration can be adjusted according to actual requirements.
Specifically, as shown in fig. 4, for an imaging image obtained in an actual operation process, the a region represents a region where an image corresponding to the entire biological sample film a acquired by the photoelectric conversion element 3 is located, a plurality of the b regions arranged vertically represent regions where marker images corresponding to markers are located, and the c region represents a region of the photoelectric conversion element excluding the biological sample film a.
The pixel value corresponding to the region a is represented by Iw, the pixel value corresponding to the region b is represented by Im, the pixel value corresponding to the region c is represented by Ih, Im < Iw < Ih, for example, the value of Im is defined as 100, the value of Iw is defined as 10, and the value of Ih is defined as 0; the higher the pixel value is, the darker the imaged color is, the more obvious and clearer the image is, and thus the sharpest and most prominent marker image corresponding to the b region is obtained.
The imaging device in the embodiment comprises a shell, light source equipment and a photoelectric conversion element, wherein a darkroom space is formed in the shell, and when the light source equipment is started, the photoelectric conversion element acquires an image corresponding to a marker on a biological sample film, so that the image acquisition of the marker on the biological sample film is realized in a contact imaging mode, and the imaging is clear; meanwhile, the imaging device has the advantages of small structure, low manufacturing cost, convenience in operation, convenience in carrying and the like.
Example 2
As shown in fig. 5, the imaging device of the pre-stained marker on the biological sample membrane of the present embodiment is a further improvement of embodiment 1, specifically:
the imaging device further comprises a protective film 6, and two sides of the protective film 6 are respectively attached to the biological sample film a and the photoelectric conversion element 3.
The outer edge of the protective film 6 is located outside the outer edge of the photoelectric conversion element 3.
Wherein, the protective film is made of toughened glass film, hard plastic film and the like.
The photoelectric conversion element 3 includes a probe body 7 and a substrate 8, and an outer edge of the substrate 8 is located outside an outer edge of the probe body 7.
The substrate is a metal plate, a PCBA plate, or the like.
As shown in fig. 6, the top of the base 5 has a receiving groove 9, and the photoelectric conversion element 3 and the protection film 6 are located in the receiving groove 9.
The imaging device of the present embodiment further includes a packing member 10 that acts on the protective film 6 and the base 5 to restrict the movement of the photoelectric conversion element 3 relative to the base 5.
Specifically, the package 10 includes a filler 11 and a card 12.
The filler 11 is located in a region surrounded by the photoelectric conversion element 3, the protective film 6, and the surface of the base 5, and is used to fix the photoelectric conversion element 3 in the base 5.
Wherein the filler is gel, glass powder, plastic embryo, etc.
When the photoelectric conversion element 3 is in the packaging state, the card board 12 abuts against the peripheral edge of the protective film 6 to ensure the stability of the whole device.
The base 5 comprises a bottom plate 13 and a base body 14, wherein the base body 14 is provided with a containing groove 9, the containing groove 9 penetrates through the base body 14, and the bottom plate 13 is movably connected to the bottom of the base body 14 so as to open or close the containing groove 9 from the bottom of the base body 14. The height of the photoelectric conversion element 3 is not greater than the depth of the accommodation groove 9.
In addition, the light source device 2 is provided at a top position inside the light shielding cover 4.
The light source device comprises a plurality of LED lamp beads arranged in a lattice shape, a plurality of lamps guided by optical fibers, a plurality of lamp tubes arranged in parallel, a plurality of plate-shaped lamps and the like.
As shown in fig. 7, the imaging device further includes a light diffusion plate 15, and the light diffusion plate 15 is fixedly disposed on the inner side of the light shielding cover 4 and located right below the light source device 2, so that the light of the light source device 2 is uniform.
The imaging device in the embodiment comprises a shell, light source equipment and a photoelectric conversion element, wherein a darkroom space is formed in the shell, and when the light source equipment is started, the photoelectric conversion element acquires an image corresponding to a marker on a biological sample film, so that the image acquisition of the marker on the biological sample film is realized in a contact imaging mode, and the imaging is clear; meanwhile, the imaging device has the advantages of small structure, low manufacturing cost, convenience in operation, convenience in carrying and the like.
Although particular embodiments of the present invention have been described above, it will be appreciated by those skilled in the art that these are examples only and that the scope of the present invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications are all within the scope of the invention.
Claims (12)
1. An imaging device for a marker prestained on a biological sample film, characterized in that the imaging device comprises a housing, a light source device, and a photoelectric conversion element;
a darkroom space is formed inside the shell;
the light source equipment and the photoelectric conversion element are arranged in the shell;
the biological sample film is arranged between the light source equipment and the photoelectric conversion element, and the biological sample film is loaded with a marker of a pre-dyed molecular weight standard sample;
wherein the light transmission of the marker is less than the light transmission of the biological sample film;
the photoelectric conversion element is used for acquiring a corresponding optical signal at the position of the marker on the biological sample film after the light source device is started, and converting the optical signal into a marker image corresponding to the marker.
2. The apparatus for imaging a pre-stained marker on a biological sample membrane as claimed in claim 1, wherein said housing comprises a light-shielding cover and a base;
one side of the shading cover is hinged with one side of the base;
when the shading cover and the base are closed, the interior of the shell forms the darkroom space.
3. The apparatus for imaging a pre-stained marker on a biological sample film according to claim 1, wherein said biological sample film is attached to a surface of said photoelectric conversion element.
4. The imaging device of a pre-stained marker on a biological sample membrane of claim 2, wherein said imaging device further comprises a protective membrane;
and two sides of the protective film are respectively attached to the biological sample film and the photoelectric conversion element.
5. The imaging device of a pre-stained marker on a biological sample membrane of claim 4, wherein said imaging device further comprises a packaging;
an outer edge of the protective film is located outside an outer edge of the photoelectric conversion element;
the top of the base is provided with a containing groove, and the photoelectric conversion element and the protective film are positioned in the containing groove;
the package member acts on the protective film and the base to restrict movement of the photoelectric conversion element relative to the base.
6. The apparatus for imaging a marker prestained on a biological sample membrane according to claim 5, wherein an outer edge of the protective membrane abuts against an inner wall surface of the accommodating chamber; and/or the presence of a gas in the gas,
the package member includes a filler located in an area surrounded by the photoelectric conversion element, the protective film, and a surface of the base member, for fixing the photoelectric conversion element in the base member.
7. The apparatus for imaging a marker prestained on a biological sample membrane according to claim 6, wherein said filler is a gel, a glass frit or a plastic embryo; and/or the presence of a gas in the gas,
the package comprises a clamping plate, and when the photoelectric conversion element is in a packaging state, the clamping plate abuts against the peripheral edge of the protective film.
8. The apparatus for imaging a pre-stained marker on a biological sample membrane according to claim 5, wherein the base comprises a bottom plate and a base body, the base body is provided with the accommodating groove, the accommodating groove penetrates through the base body, the bottom plate is movably connected to the bottom of the base body so as to open or close the accommodating groove from the bottom of the base body, and the height of the photoelectric conversion element is not greater than the depth of the accommodating groove.
9. The apparatus for imaging a marker prestained on a biological sample film as set forth in claim 8, wherein the photoelectric conversion element includes a probe body and a substrate, an outer edge of the substrate being located outside an outer edge of the probe body.
10. The apparatus for imaging a marker prestained on a biological sample membrane according to claim 9, wherein the substrate is a metal plate or a PCBA plate; and/or the presence of a gas in the gas,
the protective film is made of a toughened glass film or a hard plastic film; and/or the presence of a gas in the gas,
the photoelectric conversion element comprises a CMOS chip, a CCD chip or an amorphous silicon photoelectric conversion detector.
11. The apparatus for imaging a pre-stained marker on a biological sample membrane as claimed in claim 2, wherein said light source means is provided at a top position inside said light-shielding cover; and/or the presence of a gas in the gas,
the corresponding light-emitting duration is 10ms-1000ms after the light source equipment is started; and/or the presence of a gas in the gas,
the light source equipment comprises a plurality of LED lamp beads arranged in a lattice shape, a plurality of lamps guided by optical fibers, a plurality of lamp tubes arranged in parallel or a plurality of plate-shaped lamps.
12. The imaging device of a pre-stained marker on a biological sample film according to claim 2, wherein said imaging device further comprises a light-diffusing plate;
the light diffusion plate is fixedly arranged on the inner side of the shading cover and is positioned right below the light source equipment; and/or the presence of a gas in the gas,
the biological sample membrane comprises a protein membrane, an agarose gel block, an agarose gel strip, a polyacrylamide gel block or a polyacrylamide gel strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920416646.6U CN209858433U (en) | 2019-03-28 | 2019-03-28 | Imaging device for pre-stained markers on biological sample membrane |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920416646.6U CN209858433U (en) | 2019-03-28 | 2019-03-28 | Imaging device for pre-stained markers on biological sample membrane |
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| CN209858433U true CN209858433U (en) | 2019-12-27 |
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| CN201920416646.6U Active CN209858433U (en) | 2019-03-28 | 2019-03-28 | Imaging device for pre-stained markers on biological sample membrane |
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Address after: Room 723, building 25, Lane 3399, Kangxin highway, Pudong New Area, Shanghai, 201321 Patentee after: Eport Life Sciences (Shanghai) Co.,Ltd. Address before: 201321 room 708, building 25, Lane 3399, Kangxin highway, Pudong New Area, Shanghai Patentee before: SHANGHAI E-BLOT PHOTOELECTRIC TECHNOLOGY Co.,Ltd. |