CN209854176U - Mechanical loading device for carrying out circulating alternating strain on cells - Google Patents

Abstract

The utility model relates to a mechanical loading device for carrying out circulating alternating strain on cells, which comprises a tensile strength testing machine, a mechanical clamp and a cell loading unit; the mechanical clamp comprises an upper clamp and a lower clamp which are arranged up and down, the upper clamp comprises an upper cross beam which is transversely arranged and a pair of clamping parts which are connected with the upper cross beam in a sliding manner, each clamping part comprises a sliding block, a fixed seat and a vertically arranged chuck sliding rod which are sequentially connected from top to bottom, the chuck sliding rods are nested on the chuck sliding rods in a sliding manner, chucks are respectively detachably mounted on one sides, opposite to the fixed seats and the sliding seats, of the fixed seats, and the lower clamp is structurally the same as the upper clamp and is symmetrically arranged up and down; the cell loading unit comprises a long plate-shaped culture plate, a latex ring and a culture cover. The device is simple and convenient in sterilization and operation, can ensure the sterility of the experimental process, greatly reduce the cost, shorten the experimental period and greatly meet the requirements of users.

Description

Mechanical loading device for carrying out circulating alternating strain on cells

Technical Field

The utility model belongs to the technical field of mechanics biology, especially, relate to a mechanics loading device for being directed at cell circulation alternation meets an emergency.

Background

The mechanical factors play an important role in the aspects of human tissue growth, balance maintenance and reconstruction. The traditional in vitro cell loading methods mainly include: loading mode of single cell by using microscopic and micro-tube sucking technique; a shear stress loading mode is generated by utilizing fluid flow; a pressure loading mode is generated by utilizing flowing water gas and the like; the substrate strain loading mode and the external bioreactor are utilized to apply force to the tissues or cells in a three-dimensional state, the loading mode generally has the defect of poor stress uniformity of the cells, multiple groups of experiments cannot be carried out simultaneously, and the accuracy of the experiment result is poor; in addition, the traditional device such as a Flexcell mechanical loading machine is troublesome to operate, complex in sterilization and large in culture solution amount required by each experiment, and the test period is greatly prolonged.

Therefore, based on the problems, the device changes the previously generally-considered transverse or axial stretching stress application mode, applies tensile stress to cells in a new form of bending stress application, ensures the stress uniformity of the cells, is simple and convenient in sterilization and operation, can ensure the sterility of the experimental process, greatly reduces the cost, shortens the experimental period, greatly meets the requirements of users, is used for the mechanical loading device for circularly alternating strain of the cells, and has important practical significance.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art not enough, provide a generally regarded before changing horizontal or axial tension afterburning mode to tensile stress is applyed to the cell to crooked afterburning new form, has guaranteed the atress homogeneity of cell, and this device sterilization, the operation is all simple and convenient, can guarantee the aseptic nature of experimentation, the cost that has also significantly reduced shortens the experimental period, the mechanics loading device who is used for to circulate the alternation to the cell and meets an emergency who has greatly satisfied user's demand.

The utility model discloses take following technical scheme to realize:

a mechanical loading device for carrying out circulating alternating strain on cells comprises a tensile strength testing machine, a mechanical clamp and a cell loading unit, wherein the tensile strength testing machine is a U-shaped structure consisting of a base and support columns arranged on two sides of the base, a movable cross beam transversely arranged is arranged between the two support columns in a sliding mode, and the mechanical clamp is arranged between the movable cross beam and the base;

the mechanical fixture comprises an upper fixture and a lower fixture which are arranged up and down, the upper fixture comprises an upper cross beam which is transversely arranged and a pair of clamping parts which are in sliding connection with the upper cross beam, a T-shaped chute is formed in the lower surface of the upper cross beam along the length direction of the upper cross beam, each clamping part comprises a sliding block, a fixed seat and a vertically arranged chuck sliding rod which are sequentially connected from top to bottom, the sliding block is clamped in the T-shaped chute in a sliding mode and locked on the T-shaped chute through a locking bolt, a sliding seat is nested on the chuck sliding rod in a sliding mode and locked on the chuck sliding rod through a locking bolt, chucks are detachably mounted on one sides, opposite to the fixed seat and the sliding seat, respectively, and the lower fixture is structurally the same as the upper fixture and is arranged symmetrically up and;

the cell loading unit comprises a long plate-shaped culture plate, a latex ring arranged in the middle of the upper end of the culture plate and a culture cover covering the upper part of the latex ring.

Preferably, the material of culture plate, latex circle, cultivation lid is polystyrene, the latex circle passes through biological silica gel and glues on the culture plate, encloses to establish into the cell culture room that is used for holding the cell culture liquid.

Preferably, the chucks on the fixed seat and the sliding seat are arranged in parallel, the structure of the chuck is triangular prism, the smooth edge design is adopted on one side, opposite to the chuck, of the fixed seat and the sliding seat respectively, and the chuck is made of low-carbon steel, medium-carbon steel or sticky soft rubber.

Preferably, the locking screw is installed on the sliding seat in a threaded manner, and the tail end of the locking screw penetrates through the sliding seat and abuts against the outer wall of the chuck sliding rod.

Preferably, the part that the slider is located T type spout matches with T type spout shape, the head of locking bolt is located T type spout, just the screw rod portion of locking bolt wears out and runs through the part that the slider is located outside T type spout from T type spout, the afterbody threaded connection of locking bolt has lock nut.

Preferably, the middle parts of the sides, far away from the clamping part, of the upper clamp and the lower clamp are respectively and fixedly connected with a hollow nut, the hollow nuts on the upper clamp and the lower clamp are respectively in threaded connection with the pressing rod and the supporting rod, and one sides, far away from the hollow nuts, of the pressing rod and the supporting rod are respectively communicated with a through hole for inserting a bolt.

Preferably, the opposite side edges of the upper clamp and the lower clamp are provided with scales along the length direction.

Preferably, one side of the supporting column close to the base is provided with a sliding groove along the length direction, the sliding groove is rotatably provided with a ball screw rod arranged in parallel, and two ends of the movable cross beam are respectively connected with a driving block in threaded fit with the ball screw rod.

Further preferably, be equipped with the synchronous pivoted direct current servo control motor of two ball screws of drive in the base, direct current servo control motor passes through gear drive and is connected with ball screw transmission, gear drive includes the driving gear of assembling on direct current servo control motor shaft and assembles respectively in the driven gear of ball screw lower part, be connected through the transmission rack circle transmission between driving gear and the driven gear.

The utility model has the advantages that:

1. the mechanical clamp designed by the device can be combined with a tensile strength testing machine to realize cyclic unidirectional strain loading, the design changes the previously generally-considered transverse or axial tensile stress application mode, and applies tensile stress to cells in a new form of bending stress application, so that the stress uniformity of the cells is ensured.

2. The utility model discloses in, mechanical fixture including the last anchor clamps that set up from top to bottom, lower anchor clamps, go up the hold assembly on anchor clamps, the lower anchor clamps and be used for centre gripping cell loading unit, freely remove and confirm behind the experimental required position and latched position along T type spout through adjusting the hold assembly, can satisfy the required dependent variable of various tensile strain basically.

3. The utility model discloses in, reciprocate and confirm behind the clamping position and latched position along the chuck slide bar through adjusting the sliding seat, realize adjusting the interval between two chucks between fixing base, sliding seat, be applicable to the cell loading unit of different thickness of test, size then, and the chuck on fixing base, the sliding seat is demountable installation, is convenient for change according to the actual demand, can satisfy the demand of multiple experiment.

4. The utility model discloses in, the structure of chuck is the triangular prism shape, is the curved surface with the contact surface design of chuck and cell loading unit to guarantee to be the point with the contact surface of cell loading unit, reach the tensile effect of one-way alternation, and a plurality of cell loading units of parallel mount around on the mechanical fixture, realize then that the multiunit experiment goes on simultaneously, improve the accuracy of experimental result then.

5. The utility model designs a neotype cell loading unit, adorn cell culture liquid in the cell culture room, required culture solution volume only needs to satisfy cell growth just enough, and to a great extent has solved traditional loaded device that meets an emergency's complex operation, and required culture solution is big, and the problem of experimental period length can guarantee the aseptic nature of experimentation, the cost that has also significantly reduced shortens the experimental period.

6. The utility model discloses in, the material that the culture plate was used can supply the good adherence of cell, can cultivate with normal blake bottle is the same, and the cell adheres to the wall on the culture plate of latex circle bottom, and when the culture plate atress, the cell that adheres to the wall on the culture plate atress thereupon realizes carrying out even mechanics stimulation to the cell then, has solved the inhomogeneity of many afterburning mode cell atresss, has guaranteed the atress homogeneity of cell.

7. The utility model discloses in, go up anchor clamps, lower anchor clamps and go up additional scale both can help the experimenter to confirm the mid point of pressure head loading point at test cell loading unit, and the experimenter adjustment of can being convenient for again is gone up in the anchor clamps clamping part and the lower anchor clamps position of clamping part, guarantees that the middle zone is bending moment district such as to be convenient for the experimenter adjustment is with the accuracy of guaranteeing to meet an emergency, the accuracy nature of assurance experiment.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a perspective view of a mechanical clamp;

FIG. 3 is an enlarged view of the portion A of FIG. 2;

FIG. 4 is a front view of the mechanical fixture of FIG. 2;

FIG. 5 is a schematic left side view of the mechanical fixture of FIG. 2;

FIG. 6 is a schematic top view of the mechanical fixture of FIG. 2;

FIG. 7 is a schematic perspective view of a cell loading unit;

FIG. 8 is a schematic front view of the cell loading unit of FIG. 7;

FIG. 9 is a schematic top view of the cell loading unit of FIG. 7;

FIG. 10 is a schematic diagram of the operation of the present invention;

FIG. 11 is a schematic diagram of the mechanical fixture and cell loading unit of FIG. 10 in a loaded state;

FIG. 12 is a block diagram illustrating the operation of the present invention;

fig. 13 is a mechanical model and a bending moment diagram of fig. 11.

In the figure: 1. the cell culture device comprises a base, a support column 2, a movable cross beam 3, an upper clamp 4, a lower clamp 5, an upper cross beam 6, a sliding block 7, a fixed seat 8, a chuck sliding rod 9, a locking bolt 10, a sliding seat 11, a locking screw 12, a chuck 13, a cell loading unit 14, a culture plate 15, a latex ring 16, a culture cover 17, a hollow nut 18, a compression bar 19, a compression bar 20, a support bar 21, a hole 21, a pressure head 22, a plug 23, a sliding chute 24, a sliding chute 25, a ball screw, a driving block 26 and a T-shaped sliding chute 27.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and examples.

As shown in fig. 1-13, a mechanical loading device for performing cyclic alternating strain on cells comprises a tensile strength testing machine, wherein the tensile strength testing machine is a U-shaped structure composed of a base 1 and support columns 2 arranged at two sides of the base 1, and a movable cross beam 3 arranged transversely is arranged between the two support columns 2 in a sliding manner; the cell loading device also comprises a mechanical clamp and a cell loading unit 14, wherein the mechanical clamp is arranged between the movable cross beam 3 and the base 1; the mechanical clamp comprises an upper clamp 4 and a lower clamp 5 which are arranged up and down, the upper clamp 4 comprises an upper cross beam 6 which is transversely arranged and a pair of clamping components which are connected with the upper cross beam 6 in a sliding way, the lower surface of the upper cross beam 6 is provided with a T-shaped sliding groove 27 along the length direction, the clamping part comprises a sliding block 7, a fixed seat 8 and a vertically arranged chuck sliding rod 9 which are sequentially connected from top to bottom, the sliding block 7 is slidably clamped in the T-shaped sliding groove 27, and the slide block 7 is locked on the T-shaped slide groove 27 through a locking bolt 10, the chuck slide rod 9 is nested with a slide seat 11 in a sliding way, the sliding seat 11 is locked on the chuck sliding rod 9 through a locking screw 12, the chucks 13 are respectively and detachably mounted on the opposite sides of the fixed seat 8 and the sliding seat 11, and the lower clamp 5 is the same in structure as the upper clamp 4 and is arranged in an up-and-down symmetrical manner; the cell loading unit 14 includes a long plate-shaped culture plate 15, a latex ring 16 mounted in the middle of the upper end of the culture plate 15, and a culture cover 17 covering the upper part of the latex ring 16.

Example (b): as shown in figures 1-9, the mechanical clamp designed by the device can be combined with a tensile strength tester to realize cyclic unidirectional strain loading, so that the application range of a traditionally considered material tester is changed, and the design changes the previously generally considered transverse or axial tensile stress application mode and applies tensile stress to cells in a new mode of bending stress application; the device comprises a power source part, a mechanical clamp, a supporting part and a corresponding cell loading unit 14, wherein the mechanical clamp is arranged between a movable cross beam 3 and a base 1 and comprises an upper clamp 4 and a lower clamp 5 which are arranged up and down, the power source part above the device consists of a pressure rod 19 connected with a power source (provided for the movable cross beam 3 by a tensile strength testing machine) and the upper clamp 4, and the upper clamp 4 is connected with a pressure head 22 on the movable cross beam 3 through the pressure rod 19 so as to drive the upper clamp 4 to move up and down through the movable cross beam 3; the supporting part comprises a base 1, a supporting rod 20 connected with the base and a lower clamp 5, wherein the lower clamp is connected with a pressure head 22 on the base 1 through the supporting rod 20; the required cell loading unit/test sample is placed between the power source part and the supporting part above; the upper clamp 4 comprises an upper cross beam 6 which is transversely arranged and a pair of clamping parts which are connected with the upper cross beam 6 in a sliding way, the lower clamp 5 has the same structure as the upper clamp 4 and is symmetrically arranged up and down, the clamping parts can slide on the upper cross beam 6 so as to adjust the distance between the clamping parts at two sides, and the clamping parts on the upper clamp 4 and the lower clamp 5 are used for clamping the cell loading unit 14; the clamping part comprises a sliding block 7, a fixed seat 8 and a vertically arranged chuck sliding rod 9 which are connected in sequence, the sliding block 7 is clamped in a T-shaped sliding groove 27 in a sliding manner, after the position required by the test is determined by adjusting the clamping part to freely move along the T-shaped sliding groove 27, the sliding block 7 can be locked on the T-shaped sliding groove 27 through a rotary locking bolt 10, the strain quantity required by various tensile strains can be basically met, a sliding seat 11 is nested on the chuck sliding rod 9 in a sliding manner, after the clamping position is determined by adjusting the sliding seat 11 to move up and down along the chuck sliding rod 9, the sliding seat 11 can be locked on the chuck sliding rod 9 through a rotary locking screw 12, so that the distance between two chucks 13 between the fixed seat 8 and the sliding seat 11 can be adjusted, and the clamping part is further suitable for testing cell loading units 14 with; the contact surface between the clamping head 13 and the cell loading unit 14 is designed to be a curved surface so as to ensure that the contact surface with the cell loading unit 14 is a point, and the effect of four-point bending is achieved; before testing, the pressure heads 22 of different power sources can be selected according to the loading requirements of different cell loading units/samples to be tested, the mechanical clamp is installed on a tensile strength testing machine, the bending moment fixing slide block 7 is adjusted according to the different cell loading units/test samples to ensure that the pressure heads 22 are positioned between two clamping parts, then the test samples are clamped through the sliding seat 11 which slides according to the different thicknesses of the test samples, and then are fixed through the locking screw 12, when the cell loading unit 14 is inserted between two clamping heads of each clamping part (shown in figure 1) by adjusting the clamping heads on the clamping parts in the upper clamp 4 and the lower clamp 5 to be positioned on the same horizontal plane, pressure is applied to the cell loading unit 14 through the power source part (shown in figure 10), and the cell loading unit 14 is bent along with the force.

Wherein, the cell loading unit 14 comprises a long plate-shaped culture plate 15, an emulsion ring 16 arranged in the middle of the upper end of the culture plate 15 and a culture cover 17 covering the upper part of the emulsion ring 16, the emulsion ring 16 is adhered on the culture plate 15 through biological silica gel and is enclosed into a cell culture room for containing cell culture solution, the utility model discloses a novel cell loading unit, the cell culture solution is arranged in the cell culture room, the required culture solution amount only needs to satisfy the cell growth enough, the sterility of the experimental process can be ensured, the cost can be greatly reduced, the experimental period is shortened, and the material used for the culture plate 15 can be used for the good adherence of cells, can be cultured as a normal culture bottle, the cells adhere to the culture plate 15 at the bottom of the emulsion ring 16, when the culture plate 15 is stressed, the cells adhered to the culture plate 15 are stressed therewith, thereby realizing the mechanical stimulation of the cells, and because the cell culture chamber is arranged in the middle of the cell loading unit, the non-uniformity of cell stress in a plurality of stress application modes is solved, and the stress uniformity of the cells is ensured; the utility model discloses sterilization, operation are all simpler, and to a great extent has solved traditional loading attachment that meets an emergency's complex operation, and required culture solution is big, and the problem of experiment cycle length is long, and can realize the multiunit experiment simultaneously and go on, wherein, can install and to study the tensile back mechanics biological effect to various adherent cells (like osteoblast system, chondrocyte system, mesenchymal stem cells (BMSCs), periodontal membrane fibroblast (PDLFCs) etc.) that can all carry out the circulation tensile strain.

It should be noted that, the tensile strength testing machine of the present invention can be but not limited to use a plurality of material testing machines, such as INSTRON testing machine, and can realize cyclic unidirectional strain loading, thereby changing the application range of the material testing machine which is traditionally considered.

Still further, can also be in the utility model discloses in consider, the material of culture plate 15, latex circle 16, cultivation lid 17 is polystyrene, latex circle 16 glues on culture plate 15 through biological silica gel, encloses to establish into the cell culture room that is used for holding the cell culture liquid. The preparation method of the cell loading unit comprises the steps of adhering the latex ring 16 to the culture plate 15 through biological silica gel, vulcanizing at room temperature, covering the culture cover 17, sterilizing Co60 and then storing for later use, wherein the cell loading unit can be made of polystyrene material provided by Corning company, the culture plate 15 is made of polystyrene and is beneficial to good cell adherence, the cells adhere to the culture plate 15 at the bottom of the latex ring 16, and when the culture plate 15 is stressed, the cells adhering to the culture plate 15 are stressed along with the stress, so that uniform mechanical stimulation on the cells is realized.

Still further, can also be in the utility model discloses in consider, chuck 13 parallel arrangement on fixing base 8, the sliding seat 11, just chuck 13's structure is the triangular prism shape, the slick and sly edge design is adopted respectively to the relative one side of chuck 13 on fixing base 8, the sliding seat 11, chuck 13's material is low carbon steel, medium carbon steel, or glues soft rubber. The culture plate 15 in the cell loading unit is clamped between two chucks 13 of each clamping part on the fixed seat 8 and the sliding seat 11, the structure of each chuck 13 is triangular prism, and the smooth edge design is adopted on one side opposite to each chuck 13 respectively, so as to ensure that the contact surface of each chuck 13 and the cell loading unit is a point, and achieve the effect of unidirectional alternating stretching, and the structure of each chuck 13 is triangular prism, so that a plurality of cell loading units can be arranged on the mechanical clamp in parallel front and back, thereby realizing the simultaneous implementation of a plurality of groups of experiments, and further improving the accuracy of the experimental results; in addition, the chucks 13 on the fixed seat 8 and the sliding seat 11 are both detachably mounted, when a general metal sample or a nonmetal sample is measured, the chucks 13 are directly contacted with the sample, the chucks 13 can be made of low-cost low-carbon steel or medium-carbon steel, the mechanical property of the chucks is improved through a proper heat treatment process, and for experiments with small experimental force, for example, the chucks 13 contacted with samples such as a cell loading unit, a plastic film or a fiber wire can be made of materials such as sticky soft rubber, the chucks 13 on the fixed seat 8 and the sliding seat 11 are convenient to detach and convenient to replace according to actual requirements, and can meet the requirements of various experiments, wherein the mounting mode of the chucks 13 and the fixed seat 8 and the sliding seat 11 can be but is not limited to adhesive connection.

Still further, it is also contemplated in the present invention that the locking screw 12 is threadedly mounted on the sliding seat 11, and the tail end of the locking screw 12 passes through the sliding seat 11 and abuts against the outer wall of the chuck slide bar 9. The sliding seat 11 is nested on the chuck slide rod 9 in a sliding mode, after the clamping position is determined by adjusting the sliding seat 11 to move up and down along the chuck slide rod 9, the sliding seat 11 can be locked on the chuck slide rod 9 through rotating the locking screw 12, so that the adjustment of the distance between the two chucks 13 between the fixed seat 8 and the sliding seat 11 is achieved, and the cell loading unit 14 with different thicknesses and sizes can be tested.

Still further, can also be in the utility model discloses in consider, the part that slider 7 is located T type spout 27 matches with T type spout 27 shape, the head of locking bolt 10 is located T type spout 27, just the screw portion of locking bolt 10 is worn out and is run through the part that slider 7 is located outside T type spout 27 from T type spout 27, the afterbody threaded connection of locking bolt 10 has lock nut. After the position required by the test is determined by adjusting the free movement of the clamping member along the T-shaped sliding groove 27, the slider 7 can be locked on the T-shaped sliding groove 27 by rotating the lock nut on the lock bolt 10, and the strain amount required by various tensile strains can be basically satisfied.

Further, can also be in the utility model discloses in consider, it has a hollow nut 18 to go up anchor clamps 4, down anchor clamps 5 and keep away from one side middle part of clamping part respectively the rigid coupling, go up anchor clamps 4, down hollow nut 18 on the anchor clamps 5 respectively with depression bar 19, bracing piece 20 screw thread to link to each other, depression bar 19, bracing piece 20 keep away from one side of hollow nut 18 and link up respectively and offer and be used for bolt 23 to insert hole 21, the lower extreme middle part of movable cross beam 3, the upper end middle part of base 1 are equipped with a pressure head 22 that is used for connecting mechanical fixture respectively, depression bar 19, bracing piece 20 are inserted respectively and are located in the pressure head 22 of tensile strength testing machine and fixed mutually through bolt 23, are equipped with the pressure sensing element in the pressure head 22 of locating on movable cross beam 3, and the force sensor electricity in. The top end of the pressure lever 19 is provided with a through hole 21 for inserting a bolt 23 to fix the pressure lever 19 and a pressure head 22 on the movable cross beam into a whole, besides, the pressure lever 19 is provided with a thread which can be in threaded connection with a hollow nut 18 for fastening the pressure lever and an upper cross beam in an upper fixture into a whole, similarly, the bottom end of the support rod 20 is also provided with a through hole 21 for inserting the bolt 23 to fix the support rod 20 and the pressure head 22 on a base into a whole, the support rod 20 is provided with a thread which is in threaded connection with the hollow nut 18 for fastening the support rod 20 and the upper cross beam in a lower fixture into a.

Furthermore, the present invention also contemplates that the opposite side edges of the upper and lower clamps 4 and 5 are carved with a scale along the length direction thereof. Go up anchor clamps 4, the additional scale both can help the experimenter to confirm the pressure head loading point at the mid point of test cell loading unit on lower anchor clamps 5, in order to guarantee that loading point cross-section bending moment Mw equals PL/4's accuracy, the position of clamping part in clamping part and the lower anchor clamps in the experimenter adjustment goes up anchor clamps of being convenient for again, help the experimenter to observe the distance of clamping part in load action point (clamping part in last anchor clamps) to the lower anchor clamps at any time, guarantee that the pressure head action point is the same with the distance of clamping part in the lower anchor clamps, guarantee that the middle zone is the bending moment district such as, thereby be convenient for the experimenter to adjust in order to guarantee the accuracy of meeting an emergency, guarantee the accuracy of experiment.

Furthermore, the utility model discloses in can also consider that one side that support column 2 is close to the base has seted up a spout 24 along its length direction, 24 rotations of spout are equipped with a ball 25 that sets up with it in parallel, movable beam 3's both ends are continuous with screw-thread fit's drive block 26 on the ball 25 respectively.

It should be pointed out that be equipped with the synchronous pivoted direct current servo control motor of two ball 25 of drive in the base 1, direct current servo control motor passes through gear drive and is connected with ball transmission, gear drive includes the driving gear of assembling on direct current servo control motor shaft and assembles respectively in the driven gear of ball lower part, be connected through the transmission rack circle transmission between driving gear and the driven gear. The direct current servo control motor is used as the power source of the tensile strength testing machine, the direct current servo control motor indirectly drives the two ball screws 25 to synchronously rotate through the gear transmission mechanism, two ends of the movable beam 3 are respectively connected with the driving blocks 26 which are in threaded fit with the ball screws 25, so that the movable beam 3 can slide up and down along the ball screws under the driving of the driving blocks, and then drives the upper clamp to move up and down, and it needs to be explained that the tensile strength testing machine adopted in the utility model can be but not limited to use various material testing machines, such as an INSTRON testing machine, a base, a support column, a movable beam, a direct current servo control motor, a force sensor, a photoelectric displacement encoder, an electronic extensometer and the like which are arranged on the INSTRON testing machine, namely, the specific structure, the working principle and the circuit structure of the INSTRON testing machine are the prior art, the direct current servo control motor, the force sensor, the pressure sensing element, the photoelectric displacement encoder, the electronic extensometer and the like arranged on the device are all in the prior art in terms of specific structure, specific model, working principle and circuit structure, and are not detailed herein.

The working principle of the unidirectional alternating strain loading is briefly described below with reference to an INSTRON material testing machine, as shown in fig. 10-12: the power loading unit is characterized in that a direct current servo control motor of the INSTRON tester controls a prestressed ball screw 25 to drive the movable cross beam 3 to move so as to provide power for an upper clamp connected with the movable cross beam 3; the cell loading unit may be made of polystyrene material supplied by Corning corporation; the load and strain detection unit realizes measurement through a force sensor, a signal amplifier and a data processing system which are carried by an INSTRON tester, the most common force sensor is a strain gauge type sensor and is connected with a photoelectric displacement encoder through a series of transmission mechanisms, when the distance between an upper clamp and a lower clamp is changed, a shaft of the photoelectric displacement encoder is driven to rotate, the photoelectric displacement encoder outputs a pulse signal, and the signal is processed by a processor to obtain the deformation of a sample (a cell loading unit) and the displacement measurement of a movable cross beam. The data recording unit is formed by data acquisition and conversion through the self A/D of the INSTRON testing machine, data transmission to the microcomputer, and the microcomputer processes the data and reflects the data in the form of graphs and numerical values on the display of the microcomputer. The control panel unit inputs a strain signal to a strain signal acquisition amplifier (dynamic strain gauge), inputs a signal of the dynamic strain gauge to an INSTRON strain interface (or a multi-channel data acquisition unit), and realizes a software control process through an INSTRON controller. The device is simple and convenient to operate, the size of the load to be loaded and the size of displacement after calculation can be set on a computer, different loading waveforms and the like can be set, the requirements of users are greatly met, and the correction mechanism of the INSTRON testing machine can ensure the loading accuracy by adding the detection framework on the outer side.

Mechanical model of cyclic tensile strain loading:

the main part of the device is a unit for strain loading of cells, which consists of a four-point bending device (four clamping parts on an upper clamp and a lower clamp), and can be simplified into the following mechanical model. As shown in fig. 13:

according to the mechanics principle, the bending moment M of the CD section is M ═ Pa

In the formula: p is the load, a is the distance from the point of application of the load (the clamping part in the upper clamp) to the clamping part on the same side of the lower clamp(ii) a Since the CD section is an equal bending moment zone and the zone is a unidirectional strain state, it can be known from hooke's law that: σ ═ E ∈, as can be seen from the positive stress expression:therefore, the first and second electrodes are formed on the substrate,because E is a constant value of elastic modulus and W is a constant value of cross section moment area, the influence is large, namely the distance a from a load P and a load action point (a clamping part in an upper clamp) to a clamping part in the lower clamp on the same side of the lower clamp, the device mainly relates to A, B, C, D four points, wherein a cell loading unit (C, D respectively corresponds to the positions of two clamping parts in the lower clamp) is fixed between C, D two points, the A, B two points can apply loads to enable the cell loading unit to move up and down (A, B respectively corresponds to the positions of two clamping parts in the upper clamp), so that different strains are met, wherein the distance between C, D two points can be adjusted through two sliders 7 in the lower clamp, so that different strains are changed, therefore, the device can adjust the distance (L) between the upper and lower load P and C, D points so as to change a, thereby achieving a change in the amount of strain. The actual working strain and frequency of the cell loading unit can be calibrated in advance, and the distance between the two points of the load P and the load C, D can be adjusted according to the set magnitude.

It should be noted that the specific structure, the working principle and the circuit structure of the tensile strength testing machine used in the device are the prior art, and the tensile strength testing machine can work only by connecting the power lines of the tensile strength testing machine with the mains supply respectively, so that detailed explanation is omitted.

To sum up, the utility model provides a generally known horizontal or axial tension afterburning mode before changing to tensile stress is applyed to the cell to crooked afterburning new form, has guaranteed the atress homogeneity of cell, and this device sterilization, operation are all simple and convenient, can guarantee the aseptic nature of experimentation, and the cost that has also significantly reduced shortens the experimental period, has greatly satisfied being used for of user's demand and has circulated the mechanical loading device that the alternation is met an emergency to the cell.

The above-mentioned working principle and mechanics model are only for explaining the technical conception and features of the present invention, and the person skilled in the art can understand the contents of the present invention and implement the same, and the protection scope of the present invention cannot be limited thereby. All equivalent changes and modifications made according to the spirit of the present patent shall be covered by the protection scope of the present patent.

Claims (9)

1. The utility model provides a mechanics loading device for being directed at cell carries out circulation alternation and meets an emergency, includes tensile strength testing machine, tensile strength testing machine is by base (1), the U-shaped structure of locating support column (2) constitution of base (1) both sides, two slide between support column (2) and be equipped with movable cross beam (3) of a horizontal setting, its characterized in that: the cell loading device also comprises a mechanical clamp and a cell loading unit (14), wherein the mechanical clamp is arranged between the movable cross beam (3) and the base (1);

mechanical fixture is including last anchor clamps (4), lower anchor clamps (5) that set up from top to bottom, go up anchor clamps (4) including a horizontal top crossbeam (6) that sets up and with top crossbeam (6) sliding connection's a pair of holder part, T type spout (27) have been seted up along its length direction to the lower surface of top crossbeam (6), holder part includes from last slider (7), fixing base (8) and the chuck slide bar (9) of vertical setting that connect gradually down, slider (7) slip joint is in T type spout (27), just slider (7) lock in on T type spout (27) through locking bolt (10), it has a sliding seat (11) to go up to slide nestification on chuck slide bar (9), sliding seat (11) lock in on chuck slide bar (9) through locking bolt (12), fixing base (8) lock on chuck slide bar (9), fixing base (8), The opposite sides of the sliding seats (11) are respectively detachably provided with chucks (13), and the lower clamp (5) has the same structure as the upper clamp (4) and is arranged in a vertically symmetrical manner;

the cell loading unit (14) comprises a long plate-shaped culture plate (15), a latex ring (16) arranged in the middle of the upper end of the culture plate (15) and a culture cover (17) covering the upper part of the latex ring (16).

2. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: the material of culture plate (15), latex circle (16), cultivation lid (17) is polystyrene, latex circle (16) are pasted on culture plate (15) through biological silica gel, enclose to establish into the cell culture room that is used for holding the cell culture liquid.

3. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: chuck (13) parallel arrangement on fixing base (8), sliding seat (11), just chuck (13) structure is the triangular prism shape, the smooth and slick edge design is adopted respectively to the relative one side of chuck (13) on fixing base (8), sliding seat (11), the material of chuck (13) is low carbon steel, medium carbon steel, or glues soft rubber.

4. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: the locking screw (12) is installed on the sliding seat (11) in a threaded mode, and the tail end of the locking screw (12) penetrates through the sliding seat (11) and abuts against the outer wall of the chuck sliding rod (9).

5. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: the part that slider (7) is located T type spout (27) matches with T type spout (27) shape, the head of locking bolt (10) is located T type spout (27), just the screw portion of locking bolt (10) is worn out and is run through slider (7) and be located the part outside T type spout (27) from T type spout (27), the afterbody threaded connection of locking bolt (10) has lock nut.

6. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: go up anchor clamps (4), down anchor clamps (5) and keep away from one side middle part of clamping part and do not the rigid coupling have a hollow nut (18), go up hollow nut (18) on anchor clamps (4), lower anchor clamps (5) and link to each other with depression bar (19), bracing piece (20) screw thread respectively, one side that hollow nut (18) were kept away from in depression bar (19), bracing piece (20) link up respectively and offer and be used for bolt (23) to insert hole (21).

7. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: and scales are carved on the opposite side edges of the upper clamp (4) and the lower clamp (5) along the length direction of the upper clamp.

8. A mechanical loading device for cyclically alternating strain a cell according to claim 1 wherein: one side of the supporting column (2) close to the base is provided with a sliding groove (24) along the length direction, a ball screw (25) arranged in parallel with the sliding groove (24) is rotationally arranged in the sliding groove (24), and two ends of the movable cross beam (3) are respectively connected with a driving block (26) in threaded fit with the ball screw (25).

9. A mechanical loading device for cyclically alternating strain a cell according to claim 8, wherein: the automatic ball screw driving mechanism is characterized in that a direct current servo control motor for driving two ball screws (25) to synchronously rotate is arranged in the base (1), the direct current servo control motor is in transmission connection with the ball screws through a gear transmission mechanism, the gear transmission mechanism comprises a driving gear assembled on a motor shaft of the direct current servo control motor and driven gears assembled on the lower portions of the ball screws respectively, and the driving gear is in transmission connection with the driven gears through a transmission rack ring.