CN209841763U - Preparation device of free chlorine microelectrode - Google Patents
Preparation device of free chlorine microelectrode Download PDFInfo
- Publication number
- CN209841763U CN209841763U CN201920324437.9U CN201920324437U CN209841763U CN 209841763 U CN209841763 U CN 209841763U CN 201920324437 U CN201920324437 U CN 201920324437U CN 209841763 U CN209841763 U CN 209841763U
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- CN
- China
- Prior art keywords
- lead glass
- glass micropipette
- microelectrode
- bismuth alloy
- free chlorine
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a preparation facilities of free chlorine microelectrode, lead glass micropipette (1) including toper structure, its characterized in that: lead glass micropipette (1) in be equipped with bismuth alloy (2), bismuth alloy (2) top connect and be equipped with copper line (3), copper line (3) extend to lead glass micropipette (1) outside, bismuth alloy (2) below connect and be equipped with platinum silk (4), platinum silk (4) end sealed with the pointed end of lead glass micropipette (1) to be equipped with recess (5), recess (5) in-connection be equipped with cellulose acetate membrane (6). Compared with the prior art, the utility model the advantage lie in: the utility model can measure in the biological membrane without destroying the structure of the biological membrane, and is helpful to better understand the application of the chlorine microelectrode in situ.
Description
Technical Field
The utility model relates to a microelectrode technical field specifically indicates a preparation facilities of free chlorine microelectrode.
Background
The microelectrode refers to an electrode with a small working area, and the limitation of the area size of the electrode is not very strict. Microelectrodes include two meanings: first, miniaturization of electrodes is pointed out. Such as miniaturized ion selective electrodes, for direct observation of activity changes in body fluids and even certain important ions within cells. Glass capillary (tip diameter below 1 μm) electrodes, under micromanipulator control, were placed near the cell surface or inserted into the cell to observe the electrical activity of individual cells. Microelectrodes are a tool in medicine for the study of cells. ② refers to a kind of electrode in which the electrode area is small but the whole electrode is not miniaturized in electrochemical analysis. Such as an indicator electrode, a mercury drop electrode, a mercury-suspended electrode used in polarography and voltammetry, an indicator electrode in coulometry, a micro-platinum electrode, and the like are also referred to as microelectrodes.
An ion selective electrode is an electrochemical sensor whose potential is linear with the logarithm of the activity of a given ion in solution. Ion selective electrodes comprise a sensitive membrane composed of a specific electroactive species that is selectively responsive to the activity of certain ions in solution to produce a certain equilibrium potential. The potential difference from the reference electrode can be measured to calculate the activity of the particular ion.
Ion selective electrodes are a new analytical tool for analyzing ionic activity tension in solutions, and are one of the most rapidly developing analytical techniques over the past thirty years. Compared with other methods, the ion selective electrode method has the advantages of convenient operation, high analysis speed, wide measurement range, high sensitivity, non-destructive property and the like.
The brief structure of the microelectrode is that the process of developing the ion selective glass microelectrode is divided into 2 parts, the first part is the drawing, silanization and filling of the glass microelectrode, and the second part is the performance test of the ion selective glass microelectrode.
The method has the following defects: compared with hypochlorous acid, monochloramine has high sensitivity to anodic reaction with gold or platinum at +800mV (saturated calomel electrode (SCE) reference) applied potential. However, these electrodes are too large, for example platinum wires of 0.41mm diameter, to be applied on a small scale of 10 μm, and there are currently no commercial or developed chloramine microelectrodes for biological analysis, including direct measurement of biofilm monochloramine penetration.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the utility model is to overcome the technical defects and provide a preparation device of a free chlorine microelectrode.
In order to solve the technical problem, the utility model provides a technical scheme does: the preparation device of the free chlorine microelectrode comprises a lead glass micropipette with a conical structure, and is characterized in that: lead glass micropipette in be equipped with the bismuth alloy, bismuth alloy top connect and be equipped with the copper line, the copper line extend to the lead glass micropipette outside, bismuth alloy below connect and be equipped with the platinum silk, the tip of platinum silk end and lead glass micropipette sealed to be equipped with the recess, recess in-connection be equipped with the cellulose acetate membrane.
As an improvement, the groove is recessed by 5-6 μm.
As an improvement, the diameter of the tip of the lead glass micropipette is 5-15 μm.
Compared with the prior art, the utility model the advantage lie in: the chlorine microelectrode has a small tip of 5-15 μm, can be measured in a biological membrane without damaging the structure of the biological membrane, is favorable for better understanding the in-situ application of the chlorine microelectrode, is used for measuring monochloramine in the biological membrane of a chlorinated drinking water system, and other microelectrodes such as ammonia, pH and DO, and is favorable for explaining the nitrification phenomenon of the biological membrane.
Drawings
FIG. 1 is a schematic diagram of the structure of a free chlorine microelectrode manufacturing device of the present invention.
As shown in the figure: 1. lead glass micropipette, 2, bismuth alloy, 3, copper wire, 4, platinum wire, 5, recess, 6, cellulose acetate membrane.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
With reference to fig. 1, a device for preparing a free chlorine microelectrode comprises a lead glass micropipette 1 with a conical structure, and is characterized in that: lead glass micropipette 1 in be equipped with bismuth alloy 2, 2 tops of bismuth alloy connect and be equipped with copper line 3, copper line 3 extend to the 1 outside of lead glass micropipette, 2 below connections of bismuth alloy be equipped with platinum silk 4, 4 terminal tip sealings with lead glass micropipette 1 of platinum silk to be equipped with recess 5, 5 in-connection of recess be equipped with cellulose acetate membrane 6.
The groove 5 is sunk by 5-6 μm, and the diameter of the tip of the lead glass micropipette 1 is 5-15 μm.
The utility model discloses when the concrete implementation, cut into 4-5cm long sections with platinum silk (0.127mm diameter, 99.99% purity, Aldrich chemical Co.). The tip of the platinum wire was etched in a 6M potassium cyanide (KCN) solution with a potential of + 5V. After pulling the lead glass micropipette (o.d.: 1.5mm, i.d.: 0.75mm, 15cm length, world precision instrument), the etched platinum wire was sealed in the lead glass micropipette. Subsequently, the sealed tip was tilted to expose the platinum wire surface and resealed by heating the tip. Finally, the platinum wire tip was recessed 5-6 μm by etching in a 6MKCN solution for 1-2 seconds, and then a film was applied to the tip by dipping the tip into 10% (wt/vol) cellulose acetate in acetone for 30 seconds of the solution to prevent the stirring effect. When the microelectrode penetrates the structure of the biological film, the film provides a boundary layer of fixed thickness and also protects the clogging of the grooves, the tip diameter of the resulting microelectrode being 5-15 μm.
The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should understand that they should not be limited to the embodiments described above, and that they can design the similar structure and embodiments without departing from the spirit of the invention.
Claims (3)
1. A preparation device of free chlorine microelectrode comprises a lead glass micropipette (1) with a conical structure, and is characterized in that: lead glass micropipette (1) in be equipped with bismuth alloy (2), bismuth alloy (2) top connect and be equipped with copper line (3), copper line (3) extend to lead glass micropipette (1) outside, bismuth alloy (2) below connect and be equipped with platinum silk (4), platinum silk (4) end sealed with the pointed end of lead glass micropipette (1) to be equipped with recess (5), recess (5) in-connection be equipped with cellulose acetate membrane (6).
2. The apparatus for preparing free chlorine microelectrodes according to claim 1, wherein: the groove (5) is sunken by 5-6 μm.
3. The apparatus for preparing free chlorine microelectrodes according to claim 1, wherein: the diameter of the tip of the lead glass micropipette (1) is 5-15 mu m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920324437.9U CN209841763U (en) | 2019-03-14 | 2019-03-14 | Preparation device of free chlorine microelectrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920324437.9U CN209841763U (en) | 2019-03-14 | 2019-03-14 | Preparation device of free chlorine microelectrode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209841763U true CN209841763U (en) | 2019-12-24 |
Family
ID=68906421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920324437.9U Expired - Fee Related CN209841763U (en) | 2019-03-14 | 2019-03-14 | Preparation device of free chlorine microelectrode |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209841763U (en) |
-
2019
- 2019-03-14 CN CN201920324437.9U patent/CN209841763U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191224 |