CN209784373U - Detect Alzheimer's syndrome NCAM2 detection box - Google Patents

Abstract

A NCAM2 detection box for detecting Alzheimer's disease relates to the field of instant examination equipment, and comprises a detection card and a packaging box, wherein the detection card comprises a card box and detection test paper, the card box is made of transparent plastic and is provided with a drip opening, the bottom edge of a shell is fixedly provided with the detection test paper, and the detection test paper comprises a PVC bottom plate, a fluorescence combination pad, an NC membrane, a sample pad and a water absorption pad; the fluorescent binding pad is provided with a coated mouse anti-human NCAM2 monoclonal antibody 1-nano fluorescent microsphere compound and a chicken IgY-nano fluorescent microsphere compound, and the NC membrane is provided with a detection line formed by a mouse anti-human NCAM2 monoclonal antibody 2 and a quality control line formed by an anti-chicken IgY antibody; the packaging box comprises an upper cover, a side wall and a base, wherein a detection card is arranged on the upper side of the base, a thermometer and a hygrometer are arranged on the inner side of the upper cover, a drying cavity is formed in the packaging box, and a drying agent is arranged in the drying cavity; the application provides a detect Alzheimer's syndrome NCAM2 detection box, the dismouting of being convenient for of detection card simple structure in it, convenient operation, and avoid environmental impact.

Description

detect Alzheimer's syndrome NCAM2 detection box

Technical Field

The utility model relates to an immediate check equipment field, concretely relates to detect Alzheimer's syndrome NCAM2 detection box.

background

Alzheimer's syndrome (AD) is a progressive degenerative disease of the nervous system with occult onset. AD is one of the most common diseases in the elderly, and the World Health Organization (WHO) estimates that the prevalence rate of AD in the elderly population over 65 years old is 4-7% worldwide. The prevalence of AD is closely related to age, with an average increase in age of 1-fold for every 6.1 years.

nerve Cell Adhesion Molecules (NCAMs) are members of a large family of cell surface glycoproteins, which are expressed in the development process of the nervous system and play an important role in regulating the growth of axons, neurons also undergo synaptic plasticity change process in the process of learning and memory, and related experiments show that the NCAMs have important influence on the maintenance of long-term memory. NCAMs are selectively spliced by a single copy gene containing 26 exons to produce at least 20-30 peers. NCAMs contain structures related to immunoglobulin (Ig) and fibronectin III (FNIII) determinants and can be classified by the number of each determinant they contain, e.g., NCAM has five immunoglobulins and two FNIII determinants, while L1 glycoprotein contains six immunoglobulins and five FNIII determinants. There is a high homology between NCAMs in different species of animals, and structurally homologous molecules of NCAM and L1 have been identified in drosophila. In addition to structural changes resulting from selective splicing, NCAMs can also undergo post-translational modification processes, such as glycosylation. There are a large number of contact points between nerve cells, called synapses, which are important structures connecting brain nerve cells. During learning and memory, the neural connections of the brain need to be constantly reorganized. Loss of synapses is one of the earliest hallmark events in alzheimer's disease, and synapses are required for all brain functions, especially learning and memory formation. Synaptic loss in alzheimer's disease occurs very early (at the time of mild cognitive dysfunction in the patient), much earlier than the death of nerve cells.

The neural cell adhesion molecule 2(NCAM2) is a protein that connects synapses and stabilizes long-term synaptic connections between neural cells. Researchers have studied this protein in alzheimer's disease and normal brain tissue. Studies have shown that synaptic levels of NCAM2 are low in hippocampus of alzheimer's patients. Researchers have also shown, through mouse and in vitro experiments, that β -amyloid is able to disrupt NCAM2, resulting in synaptic loss. The level of NCAM2 protein can reveal the risk of Alzheimer's disease of individuals, can clarify the development of mild cognitive impairment of individuals, and is also important for developing effective intervention measures. The existing detection card for detecting the Alzheimer's disease NCAM2 is of an integrated structure, is inconvenient to disassemble and replace detection test paper, can be used after being disassembled from a packaging box, and is easy to be influenced by temperature and humidity.

SUMMERY OF THE UTILITY MODEL

In order to solve the not enough among the prior art, the application provides a detect Alzheimer's syndrome NCAM2 detection box, the simple dismouting of being convenient for of detection card simple structure in it, convenient operation, and avoid environmental impact.

In order to realize the technical effect, the utility model discloses a concrete technical scheme as follows:

the NCAM2 detection box for detecting the Alzheimer's disease comprises a detection card and a packaging box, wherein the detection card comprises a card box and detection test paper, the card box comprises a box cover and a box bottom which are mutually overlapped, the box bottom comprises a shell with a closed bottom edge and a closed side edge and an open top end, a concave step part is arranged on the inner side of the top end of the side edge of the shell, a notch is formed in the right side of the shell, the bottom end of the notch is lower than the horizontal plane of the step part, an overlapping part matched with the step part is arranged on the edge of the bottom surface of the box cover, a convex block matched with the notch is arranged on the right side of the box cover, the box cover is made of transparent plastic and provided with a drip opening, the bottom edge of the shell is fixedly provided with the detection test paper, and the detection test paper comprises a PVC bottom plate, a fluorescent; the sample pad, the fluorescence combination pad, the NC membrane and the water absorption pad are fixed on the PVC bottom plate in the horizontal direction, the fluorescence combination pad is coated with a mouse anti-human NCAM2 monoclonal antibody 1-nano fluorescent microsphere compound and a chicken IgY-nano fluorescent microsphere compound, and the NC membrane is provided with a detection line formed by a mouse anti-human NCAM2 monoclonal antibody 2 and a quality control line formed by an anti-chicken IgY antibody; when the box cover is lapped on the upper end of the box bottom, the liquid dropping port is positioned right above the sample pad; the packing carton includes upper cover, lateral wall and base, and the base upside is equipped with the detection card, and the upper cover inboard is equipped with thermometer and hygrometer, and the packing carton is equipped with the drying chamber, and the drying intracavity is equipped with the drier.

furthermore, a plurality of positioning seats are arranged on the bottom edge of the shell, positioning holes are formed in each positioning seat, positioning columns corresponding to the positioning seats in number are arranged on the bottom surface of the box cover, and the positioning columns are matched with the positioning holes in shape and size and can be inserted into the positioning holes in a one-to-one correspondence mode. Through the design, the positioning column is inserted into the positioning seat, so that the lap joint mode of the box cover and the box bottom is quick and stable, and the box cover is supported.

Furthermore, the detection card is of a structure with one end being rectangular and the other end being circular arc-shaped, and the notch and the bump are arranged at one circular arc-shaped end of the detection card. Breach, lug setting are in the convex one end of short-term test card, the convex structure is smooth and smooth, does not have sharp-pointed edges and corners, when separating the lid from the box bottom with the help of the lug with the finger, the finger is felt rather than the experience of contact, also can not take place probably by the potential danger of edges and corners cut, fish tail.

Furthermore, a sealing gasket is arranged between the upper cover and the side wall.

further, the liquid dropping port is of an inwards concave bowl-shaped structure. When the sample drips on the test paper through the drip mouth, the drip mouth of bowl-shaped structure plays temporary storage, memory function, and on the pore of sample through drip mouth bottom slowly permeated test paper, the sample also was difficult for overflowing outside the drip mouth.

According to the above technical scheme, compared with the prior art, the utility model, have following advantage:

1. The utility model is provided with the detection box with the drying cavity, thereby solving the technical problem that the detection result can be influenced when the detection card is placed after being opened, and preventing the detection card from being influenced by the moisture in the air and the pH value of the air;

2. The detection card needs to be used at normal temperature, and a thermometer in the detection box is convenient for reading the temperature of the detection card; the thermometer and the hygrometer are arranged in the utility model, so that the temperature and the humidity of the test can be conveniently recorded;

3. The utility model can realize rapid detection for detecting the Alzheimer's disease NCAM2 through the specially arranged detection test paper, and has convenient and rapid operation;

4. the box cover in the utility model is made of transparent plastic, only a drip opening is needed to be arranged, the whole detection process and the detection result can be directly observed through the box cover without additionally arranging an observation opening, the manufacturing process and time of the box cover are reduced, and the integral strength is better;

5. The box bottom of the utility model is provided with a step part, the box cover is provided with a lap joint part matched with the step part, when the box cover is lapped on the box bottom, the edge of the box cover is flush with the top end of the side edge of the box bottom, on one hand, the thickness of the whole box body is reduced, the volume is reduced, on the other hand, the whole box cover is surrounded by the side edge of the box bottom, and the lap joint of the box cover and the box bottom is more stable;

6. The box bottom of the utility model is provided with a gap, the box cover is provided with a lug which is matched with the gap, and when the box cover is disassembled from the box bottom, the disassembly is more convenient by means of the lug;

7. The utility model discloses well box body through reference column on the lid, the locating hole on the box bottom, realizes the high-speed joint, convenient and fast, the equipment of the detection card of being convenient for, dismantlement.

Drawings

the present invention will be described in further detail with reference to the following detailed description and accompanying drawings.

Fig. 1 is a top view of the present invention;

Fig. 2 is a side view of the present invention;

FIG. 3 is a schematic view of the structure of the detection card of the present invention;

FIG. 4 is a schematic view of the bottom structure of the detection card of the present invention;

FIG. 5 is a schematic view of a structure of a cover of a detection card of the present invention;

FIG. 6 is a schematic view of the test paper of the present invention;

wherein, 1, a box cover; 2. a box bottom; 3. a housing; 4. a step portion; 5. a notch; 6. a bump; 7. positioning seats; 8. positioning holes; 9. a lap joint section; 10. a positioning column; 11. a drip port; 12. detecting test paper; 121. A PVC base plate; 122. a fluorescent binding pad; 123. NC film; 124. a sample pad; 125. a water absorbent pad; 126. mouse anti-human NCAM2 monoclonal antibody 1-nano fluorescent microsphere compound; 127. chicken IgY-nano fluorescent microsphere compound; 128. detecting lines; 129. a quality control line; 13. detecting the card; 14. packaging boxes; 141. an upper cover; 142. a side wall; 143. a base; 15. a thermometer; 16. a hygrometer; 17. and a drying cavity.

Detailed Description

In order to make the objects, technical solutions and advantages of the present embodiments more clear, the technical solutions in the present embodiments will be clearly and completely described below with reference to the drawings in the present embodiments, and it is obvious that the described embodiments are some, but not all embodiments. All other embodiments that can be derived by a person skilled in the art from the embodiments given herein without making any creative effort fall within the scope of the present protection.

In the description herein, it is to be understood that the terms "bottom", "side", "top", "right", "left", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be construed as limiting the present invention.

In the present application, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, releasably connected, or integral; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present context can be understood by those of ordinary skill in the art as appropriate.

examples

Referring to fig. 1 and 2, a detection box for detecting the alzheimer's disease NCAM2 comprises a detection card 13 and a packaging box 14, as shown in fig. 3, the detection card 13 comprises a card box and a detection test paper 12, the card box comprises a box cover 1 and a box bottom 2 which are overlapped with each other, as shown in fig. 4, the box bottom 2 comprises a shell 3 with a closed bottom edge and a closed side edge and an open top end, a concave step part 4 is arranged on the inner side of the top end of the side edge of the shell 3, a notch 5 is arranged on the right side of the shell 3, the bottom end of the notch 5 is lower than the level of the step part 4, so that when the protrusion 6 is overlapped on the notch 5, a slit is left between the protrusions, which is convenient for inserting finger nails or other tools when the box cover 1 is detached from the box bottom 2, as shown in fig. 5, the bottom edge of the box cover 1 is provided with an overlapping part 9 which is matched with the step part 4, and the right side of the protrusion, the box cover 1 is made of transparent plastic and is provided with a dropping opening 11, the dropping opening 11 is of an inwards concave bowl-shaped structure, the detection card 13 is of a structure with one end being rectangular and the other end being circular arc-shaped, and the notch 5 and the lug 6 are arranged at one circular arc-shaped end of the detection card. The packing box 14 comprises an upper cover 141, a side wall 142 and a base 143, a sealing gasket (not shown) is arranged between the upper cover 141 and the side wall 142, the upper side of the base 143 is provided with a detection card 13, the inner side of the upper cover 141 is provided with a thermometer 15 and a hygrometer 16, the packing box 14 is provided with a drying cavity 17, and a drying agent (not shown) is arranged in the drying cavity 17.

a detection test paper 12 is fixed on the bottom edge of the housing 3, as shown in fig. 6, the detection test paper 12 includes a PVC base plate 121, a fluorescent binding pad 122, an NC film 123, a sample pad 124 and a water absorption pad 125; the sample pad 124, the fluorescence binding pad 122, the NC membrane 123 and the water absorption pad 125 are fixed on the PVC bottom plate 121 in the horizontal direction, the fluorescence binding pad 122 is coated with a mouse anti-human NCAM2 monoclonal antibody 1-nano fluorescent microsphere compound 126 and a chicken IgY-nano fluorescent microsphere compound 127, and the NC membrane is provided with a detection line 128 formed by a mouse anti-human NCAM2 monoclonal antibody 2 and a quality control line 129 formed by an anti-chicken IgY antibody; when the box cover 1 is lapped on the upper end of the box bottom 2, the dropping hole 11 is positioned right above the sample pad 124.

Three groups of positioning seats 7 are symmetrically arranged on the bottom edge of the shell 3, positioning holes 8 are arranged in each positioning seat 7, positioning columns 10 corresponding to the positioning seats 7 in number are arranged on the bottom surface of the box cover 1, and the positioning columns 10 are matched with the positioning holes 8 in shape and size and can be inserted into the positioning holes 8 in a one-to-one correspondence mode.

The above description is provided by way of example only to aid understanding of the present invention, and is not intended to limit the present invention. For a person skilled in the art, several simple deductions, modifications or substitutions may be made according to the present idea.

Claims (5)

1. The NCAM2 detection box for detecting the Alzheimer's disease is characterized by comprising a detection card and a packaging box, wherein the detection card comprises a card box and detection test paper, the card box comprises a box cover and a box bottom which are mutually overlapped, the box bottom comprises a shell, the bottom edge and the side edge of the shell are both closed, the top end of the shell is provided with an opening, the inner side of the top end of the side edge of the shell is provided with a downward-concave step part, the right side of the shell is provided with a notch, the bottom end of the notch is lower than the horizontal plane of the step part, the edge of the bottom surface of the box cover is provided with an overlapping part matched with the step part, the right side of the box cover is provided with a convex block matched with the notch, the box cover is made of transparent plastic and provided with a drip opening, the bottom edge of the shell is fixedly provided with the detection test paper, and; the sample pad, the fluorescence combination pad, the NC membrane and the water absorption pad are fixed on the PVC bottom plate in the horizontal direction, the fluorescence combination pad is coated with a mouse anti-human NCAM2 monoclonal antibody 1-nano fluorescent microsphere compound and a chicken IgY-nano fluorescent microsphere compound, and the NC membrane is provided with a detection line formed by a mouse anti-human NCAM2 monoclonal antibody 2 and a quality control line formed by an anti-chicken IgY antibody; when the box cover is lapped on the upper end of the box bottom, the liquid dropping port is positioned right above the sample pad; the packing carton includes upper cover, lateral wall and base, and the base upside is equipped with the detection card, and the upper cover inboard is equipped with thermometer and hygrometer, and the packing carton is equipped with the drying chamber, and the drying intracavity is equipped with the drier.

2. The NCAM2 testing cassette for testing Alzheimer's disease according to claim 1, wherein a plurality of positioning seats are disposed on the bottom edge of the housing, each positioning seat has a positioning hole therein, the bottom surface of the cassette cover has a number of positioning posts corresponding to the positioning seats, the positioning posts match with the positioning holes in shape and size and can be inserted into the positioning holes in a one-to-one correspondence manner.

3. The NCAM2 detection kit for detecting Alzheimer's disease according to claim 2, wherein said detection card has a rectangular shape at one end and a circular arc shape at the other end, and said notch and protrusion are disposed at one end of the circular arc shape.

4. The kit for detecting the Alzheimer's disease NCAM2 of claim 3, wherein a sealing gasket is disposed between the upper cover and the sidewall.

5. The NCAM2 test kit for detecting Alzheimer's disease according to claim 4, wherein the drip opening is a concave bowl-shaped structure.