CN209727957U - A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit - Google Patents

A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit Download PDF

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Publication number
CN209727957U
CN209727957U CN201920438230.4U CN201920438230U CN209727957U CN 209727957 U CN209727957 U CN 209727957U CN 201920438230 U CN201920438230 U CN 201920438230U CN 209727957 U CN209727957 U CN 209727957U
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reagent
bottle
calculi
reagent bottle
qualitative analysis
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CN201920438230.4U
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黄克和
王育林
袁征
孙嘉豪
潘翠玲
陈兴祥
甘芳
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Nanjing Agricultural University
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Nanjing Agricultural University
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Abstract

The utility model discloses a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kits, including box body and box cover;The box body includes: a liner, is arranged in the box body, is provided with gasket groove on the liner;At least one reagent bottle, the reagent bottle are adapted to the gasket groove;One accommodating chamber, enclosing are arranged between the liner and the medial surface of box body;One suction pipe is placed in the accommodating chamber;One sample spoon is placed in the accommodating chamber;One or six hole porcelain drip plate, are placed in the accommodating chamber.In this dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit based on chemical drop methods, it is sufficiently used the inner space of box body, all medicaments that need to be used and tool are focused in single cartridge bodies, no longer need to operate using external equipment and professional technician, carry with it is easy to use.

Description

A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit
Technical field
The utility model relates to Medical pet apparatus field, specially a kind of dog cat calculi in urinary system ingredient fast qualitative Assay kit.
Background technique
Dog cat urolithiasis is a kind of clinically common systemic nutrition metabolic diseases.The disease incidence of dog cat urolithiasis accounts for about dog 30% or so of cat disease in the urological system, clinical symptoms are frequent micturition, urgent urination, and when urination exerts duty, blood urine etc. occur, can give illness Lead for animal carrys out greatly pain.It is named as different types of urinary calculus according to the difference of urinary calculus ingredient, wherein only urinating The urinary calculus of hydrochlorate and cystine ingredient can carry out conservative therapy by drug, other are such as struvite calculus (guanite), grass Sour calcium calculus etc. largely needs to take out by operation method.
Dog cat urolithiasis has higher recurrence rate simultaneously, so infected animal needs to continue to take medicine after surgery and take The measure of some anti-recurrences, and according to the difference of stone composition, anti-recurrence measure has huge difference.Therefore, if doctor Can apparent calculus after surgery main component, targeted measure can be taken, obtain that better effect, to reduce urolithiasis multiple A possibility that hair.
Currently, what the chemical component of canine cat urinary calculi detected the most commonly used method is infrared spectroscopy, but this method needs Will be by large-scale instrument, and most pet clinic will not purchase the equipment, need sample carrying out laboratory inspection, this Three to five days time is needed, the process for the treatment of is also objectively affected.And this kit is matched based on chemical drop methods Chemical analysis reagent processed, and determine detection program engaged therewith, it is equipped with corresponding reaction vessel, so that kit is had reaction fast Speed, it is easy to operate, it is low in cost, it is easy to carry the advantages that, effectively to meet pet doctor for the fast qualitative point of urinary calculus The demand of analysis provides help for clinically prevention and treatment dog cat urolithiasis.
Utility model content
The utility model in order to solve defect existing in the prior art, provide a kind of at low cost, detection quickly, operation letter Just, the dog cat calculi in urinary system of qualitative analysis detection can be carried out to the urinary calculus chemical component of frequent species in pet clinic Ingredient fast qualitative analysis utilizing kit.
In order to achieve the above object, the utility model provides a kind of dog cat calculi in urinary system ingredient fast qualitative point first Analyse kit, including box body and box cover;Wherein, the box body includes:
One liner, is arranged in the box body, is provided with gasket groove on the liner;
At least one reagent bottle, the reagent bottle are adapted to the gasket groove;
One accommodating chamber, enclosing are arranged between the liner and the medial surface of box body;
One suction pipe is placed in the accommodating chamber;
One sample spoon is placed in the accommodating chamber;
One or six hole porcelain drip plate, are placed in the accommodating chamber.
The utility model also provides following optimisation technique scheme:
Preferably, the reagent bottle include uric acid reagent bottle I, uric acid reagent bottle II, ammonium reagent bottle, cystine reagent bottle, Phosphoric Acid bottle, hydrochloric acid reagent bottle, oxalic acid reagent bottle, calmagite bottle, azoviolet bottle, teat glass one or more of them.
Preferably, the engaging for further including the two engaging items being symmetricly set on right above liner and being adapted to the engaging item Plate;
The engaging item includes Cross slat and the support column for being connected to Cross slat bottom surface;The Cross slat side is provided with card Close slot;The connecting hole being arranged on the support column and liner, which is adapted to, to be plugged together;
The fastening plate includes the convex block and intermediate plate of two sides;The convex block and engaging slot sliding adaptation;In described Between board bottom face be provided with reagent bottle card slot.
It is furthermore preferred that be provided with two limited blocks on the box cover, the limited block lid plugged together when closing fastening plate two sides with Limit the lateral displacement of the fastening plate.
It is furthermore preferred that being additionally provided with pressing plate on the box cover, it is arranged between limited block, lid presses the engaging when lid closes Plate.
It is furthermore preferred that the limited block is set as rectangular or arc.
It is furthermore preferred that the pressing plate is set as rectangular.
It is furthermore preferred that the liner is paper liner.
Preferably, reagent is sodium carbonate liquor in the uric acid reagent bottle I, and reagent is that phosphotungstic acid is molten in uric acid reagent bottle II Liquid, reagent is Nessler's reagent in ammonium reagent bottle, and reagent is ethanol solution of ninhydrin, examination in Phosphoric Acid bottle in cystine reagent bottle Agent is ammonium molybdate-nitric acid solution, and examination is is hydrochloric acid solution in hydrochloric acid reagent bottle, and reagent is sodium acetate solution in oxalic acid reagent bottle, Reagent is sodium hydroxide solution in calmagite bottle, and reagent is azo violet in azoviolet bottle.
Preferably, six hole porcelain drop plate is provided with two rows of each three recessed grooves for waiting gaps;The teat glass specification For 5ml, quantity is four.
The beneficial effects of the utility model are:
In this dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit based on chemical drop methods, fully utilize The inner space of box body, all medicaments that need to be used and tool is focused in single cartridge bodies, it is no longer necessary to be set using outside Standby and professional technician operates, carry with it is easy to use.Kit has low in cost, detection calculus A wide selection of colours and designs, Sample requirements are few, and one-time detection total only needs 30mg or so, rapid reaction and naked eyes as it can be seen that it is easy to operate the advantages that, Be conducive to the fast qualitative analysis utilizing for completing urinary calculus chemical component in dog cat urolithiasis at lower cost.And then effectively meet pet Doctor provides help for the demand of the fast qualitative analysis utilizing of urinary calculus for clinically prevention and treatment dog cat urolithiasis.This kit energy Fast qualitative analysis utilizing rapidly and accurately is carried out to the main component of canine cat urinary calculi, is suitable for each pet clinic to dog cat urinary calculi Disease carries out Precise Diagnosis.Since the calculus of heterogeneity has different control methods, thus the testing result of this kit can be Doctor issues the anti-calculus diet offer important evidence of prevention and treatment prescription and pet owner preparation for specific calculus.This kit can It is high depending on change degree, can with naked eyes directly discriminatory analysis as a result, have the characteristics that low in cost, easy to operate, the reaction time is short, There is good market application prospect.And this dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit can solve original examination Reagent bottle pours down or forth hidden danger in agent box, can by reagent bottle it is stable a possibility that be fastened on fixed position, reduce its shaking.
Detailed description of the invention
Fig. 1 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The perspective view of box;
Fig. 2 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The perspective view of box;
Fig. 3 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The front elevation of box;
Fig. 4 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The perspective view of box;
Fig. 5 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The side view of box;
Fig. 6 is a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing reagent of preferred embodiment of the utility model The top view of box;
Fig. 7 is a kind of perspective view of the local box body of preferred embodiment of the utility model;
Fig. 8 is a kind of front view of the local box body of preferred embodiment of the utility model;
Fig. 9 is a kind of side view of the local box body of preferred embodiment of the utility model;
Figure 10 is a kind of perspective view of the local box body of preferred embodiment of the utility model;
Specific appended drawing reference are as follows:
1 box body;2 box covers;11 liners;12 gasket grooves;13 reagent bottles;14 accommodating chambers;15 suction pipes;16 sample spoons;17 6 Hole porcelain drips plate;18 engaging items;19 fastening plates;21 limited blocks;22 pressing plates;111 connecting holes;181 Cross slats;182 support columns;183 Engaging slot;191 convex blocks;192 intermediate plates;193 reagent bottle card slots.
Specific embodiment
In order to make those skilled in the art more fully understand the technical solution of utility model, below with reference to specific embodiment party The utility model is described in further detail for formula.
A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit, including box body 1 and box cover 2;Wherein, described Box body 1 includes:
One liner 11, is arranged in the box body 1, is provided with gasket groove 12 on the liner 11;The liner 11 is set It sets on the bottom surface of 1 inside of box body;
At least one reagent bottle 13, the reagent bottle 13 are adapted to the gasket groove 12;
One accommodating chamber 14, enclosing are arranged between the liner 11 and the medial surface of box body 1;Before the left and right of the liner 11 It is not directly coupled afterwards with box body 1, thus forms the accommodating chamber 14 of a recess;
One suction pipe 15 is placed in the accommodating chamber 14;
One sample spoon 16 is placed in the accommodating chamber 14;
One or six hole porcelain drip plate 17, are placed in the accommodating chamber 14.
The reagent bottle 13 includes uric acid reagent bottle I, uric acid reagent bottle II, ammonium reagent bottle, cystine reagent bottle, phosphoric acid examination Agent bottle, hydrochloric acid reagent bottle, oxalic acid reagent bottle, calmagite bottle, azoviolet bottle, teat glass one or more of them.Wherein, it urinates The reagent loaded in acid reagent bottle I is sodium carbonate liquor, and the reagent loaded in uric acid reagent bottle II is Salkowski's solution, ammonium reagent The reagent loaded in bottle is Nessler's reagent, and the reagent loaded in cystine reagent bottle is ethanol solution of ninhydrin, Phosphoric Acid bottle The reagent of interior loading is ammonium molybdate-nitric acid solution, and the reagent loaded in hydrochloric acid reagent bottle is hydrochloric acid solution, and oxalic acid reagent bottle is built-in The reagent of load is sodium acetate solution, and the reagent loaded in calmagite bottle is sodium hydroxide solution, the examination loaded in azoviolet bottle Agent is azo violet.
The fastening plate for further including the two engaging items 18 being symmetricly set on right above liner 11 and being adapted to the engaging item 18 19;
The engaging item 18 includes Cross slat 181 and the support column 182 for being connected to 181 bottom surface of Cross slat;The Cross slat 181 sides are provided with engaging slot 183;The connecting hole 111 being arranged on the support column 182 and liner 11, which is adapted to, to be plugged together;
The Cross slat 181 is preferably arranged to extend with the length direction of liner 11, and keeps one with the length of liner 11 It causes, the medial surface of Cross slat 181 is arranged in the engaging slot 183, and is higher than the height of box body 1, in order to inserting for fastening plate 19 Enter, support column 182 is preferably provided at four corner locations of liner 11;
The fastening plate 19 includes the convex block 191 and intermediate plate 192 of two sides;The convex block 191 and the engaging slot 183 are sliding Dynamic adaptation;192 bottom surface of intermediate plate is provided with reagent bottle card slot 193;
The convex block 191 of 19 two sides of fastening plate is consistent with the length of engaging slot 183, fastening plate 19 be preferable to provide for The length of liner 11 is consistent.
Two limited blocks 21 are provided on the box cover 2, the lid of limited block 21 is plugged together in 19 two sides of fastening plate when closing to limit Make the lateral displacement of the fastening plate 19.The limited block 21 is arranged in 2 position of box cover corresponding with the accommodating chamber 14, this Sample is slidably inserted into accommodating chamber 14 after so that limited block 21 is overturn, and keeps off in the two sides of fastening plate 19.
It is additionally provided with pressing plate 22 on the box cover 2, is arranged between limited block 21, lid presses the fastening plate 19 when lid closes.
The limited block 21 is set as rectangular or arc.
The pressing plate 22 is set as rectangular.
The liner 11 is paper liner.
Reagent is sodium carbonate liquor in the uric acid reagent bottle 13 I, and reagent is Salkowski's solution in uric acid reagent bottle 13 II, Reagent is Nessler's reagent in ammonium reagent bottle 13, and reagent is ethanol solution of ninhydrin, Phosphoric Acid bottle 13 in cystine reagent bottle 13 Interior reagent is ammonium molybdate-nitric acid solution, and examination is is hydrochloric acid solution in hydrochloric acid reagent bottle 13, and reagent is acetic acid in oxalic acid reagent bottle 13 Sodium solution, reagent is sodium hydroxide solution in calmagite bottle 13, and reagent is p-nitrophenyl azo isophthalic two in azoviolet bottle 13 Phenol.
Porcelain drop plate 17 in six hole is provided with two rows of each three recessed grooves for waiting gaps;The teat glass specification is 5ml, Quantity is four.
The application method of the dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit of the utility model is:
After first reagent bottle 13 is totally placed in gasket groove 12, suction pipe 15, sample spoon 16, six hole porcelain drop plate 17 are put It sets in accommodating chamber 14, then support column 182 is inserted into connecting hole 111, symmetrically insertion pads 11 by two engaging items 18 On, then the convex block 191 of fastening plate 19 is inserted into, and slide into the position of reagent bottle 13 always from engaging slot 183, is stuck in reagent The two sides of the bottle cap of bottle 13, prevent it from shaking, then along the length direction of liner 11 by fastening plate 19 from two engaging items 18 Engaging slot 183 between slide into and engage it is good after, box cover 2 is closed toward lower cover, limited block 21 first can be along the left and right of liner 11 In the space insertion box body 1 of the accommodating chamber 14 of two sides, and fastening plate 19 is limited in fixed position from the two sides of fastening plate 19, And continuing to be inserted into during lid closes into accommodating chamber 14, pressing plate 22 is also contacted with the upper surface of fastening plate 19, makes it from upper Side and after the left and right sides completely fixes, so that making the displacement possibility of reagent bottle 13 also significantly reduces, reduce its because It shakes the inclination generated and reveals and occur.
The detection program of this kit are as follows:
1, sample treatment: the calculus that operation is taken out is cleaned completely, then with after distilled water repeated flushing, is put into baking oven 50 DEG C dry 3h, complete it is dry after take out, cut fraction, mortar wears into fine powder, and remaining calculus can be packed into valve bag preservation.If When dividing calculus, section is well arranged, and layering detects respectively.Note: a calculus sample can only use a set of mortar.
2, analytical procedure:
1) calculus powder is taken with curetteearpick, every secondary control sample size is about 5mg, and 3 for being loaded onto white porcelain drop plate respectively are recessed In shape hole and 3 small test tubes, number is hole 1,2,3 and test tube 1,2,3 respectively, is strictly operated by following process sequence.
2) in hole 1, each 2 drop of uric acid reagent I and II is instilled, blue occur proves that result is the positive.
3) it in test tube 1, instills cystine reagent 3 and drips, boiling water bath 2 minutes, bluish violet occur for the positive.
4) in test tube 2, instill Phosphoric Acid 3 drip, gently shake, solution turned yellow or have yellow mercury oxide occur prove result For the positive.
5) it instills hydrochloric acid reagent 2 in test tube 3 to drip, having bubble to generate proves result for the positive.
6) in the test tube 3 used in step 5), continue to be slowly added into the drop of oxalic acid reagent 5, there is white precipitate to occur proving knot Fruit is the positive.
7) drop of hydrochloric acid reagent 1 is successively instilled in test tube 4, calmagite 3 drips, and has white precipitate to occur proving result for sun Property.
8) in the test tube 4 used in step 7), the drop of azoviolet 2 is continuously added, is rocked uniformly, precipitating becomes the bright result of blue-card as sun Property.
3, result judgement: reference table 1, wherein gauge outfit is the number of operating process, and "+" indicates the knot that the step operational observations arrive Fruit is the positive, and "-" indicates the result observed for feminine gender.Such as calculus sample detection result phosphate, ammonium salt, magnesium ion are sun Property, other detection projects are feminine gender, i.e. the class urinary calculus that is determined as ammonium magnesium phosphate ingredient;If calculus sample detection result oxalic acid Salt, calcium ion are the positive, other are feminine gender, then can determine that as the urinary calculus of oxalic acid calcium component.It can be with reference table in practical operation 1, wherein gauge outfit is the number of operating process, and "+" indicates the result that the step operational observations arrive as the positive, and "-" expression is observed It as a result is feminine gender.There is multilayer if it is calculus, suspected of the calculus of blending constituent, then need to carry out detection and interpretation respectively.
The 1 inspection result table of comparisons of table
The above is the utility model preferred embodiment, is not constituted to scope of protection of the utility model Limitation.
The utility model relates to testing principle be summarized as follows:
The main foundation of the detection method of this kit is drop method.Drop method is effect of the style powder in detection reagent Under, it is allowed to form the sediment of special crystallization or different colours, according to these crystallizations or the peculiar sign of sediment, determines knot Stone ingredient.
The testing principle of uric acid or uric acid salt component: uric acid can be oxidized to allantoin and two in alkaline environment by phosphotungstic acid Carbonoxide, phosphotungstic acid are reduced into tungsten blue.
The testing principle of ammonium salt ingredient: Nessler's reagent can react in alkaline solution with ammonium ion, and increase with its content Solution will appear yellow or even red-brown precipitation.
Cystine composition detection principle: under heating condition and weakly acidic condition, the calculus and ninhydrin of cystine ingredient are anti- Hyacinthine compound should be generated.
Phosphate component testing principle: phosphate anion generates the molybdenum ammonia-sinking of yellow phosphorus with ammonium molybdate reaction in the presence of phosphoric acid It forms sediment.
Carbonate components testing principle: the calculus of carbonate containing composition can generate titanium dioxide during diluted hydrochloric acid dissolution Carbon bubble.
Oxalates composition detection principle: when pH is 3, it is heavy that calcium ion can be combined into white calcium oxalate with oxalic ion It forms sediment.
Calcium ion and magnesium ion composition detection principle: stone composition dissolves after dilute hydrochloric acid is added, and discharges calcium ion or magnesium Ion, adds sodium hydroxide formation calcium hydroxide or magnesium hydroxide, the two are slightly soluble in water, can form precipitating;P-nitrophenyl nitrogen Resorcinol, can be reacted with magnesium hydroxide generate blue precipitate, without with calcium hydroxide reaction, to distinguish magnesium ion and calcium Ion.
Above are merely preferred embodiments of the utility model, it is noted that above-mentioned preferred embodiment should not regard For limitations of the present invention, the protection scope of the utility model should be defined by the scope defined by the claims..For For those skilled in the art, without departing from the spirit and scope of the utility model, it can also make several Improvements and modifications, these improvements and modifications also should be regarded as the protection scope of the utility model.

Claims (10)

1. a kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit, it is characterised in that: including box body and box cover;Its In, the box body includes:
One liner, is arranged in the box body, is provided with gasket groove on the liner;
At least one reagent bottle, the reagent bottle are adapted to the gasket groove;
One accommodating chamber, enclosing are arranged between the liner and the medial surface of box body;
One suction pipe is placed in the accommodating chamber;
One sample spoon is placed in the accommodating chamber;
One or six hole porcelain drip plate, are placed in the accommodating chamber.
2. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 1, it is characterised in that: institute Stating reagent bottle includes uric acid reagent bottle I, uric acid reagent bottle II, ammonium reagent bottle, cystine reagent bottle, Phosphoric Acid bottle, hydrochloric acid examination Agent bottle, oxalic acid reagent bottle, calmagite bottle, azoviolet bottle, teat glass one or more of them.
3. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 1, it is characterised in that: also The fastening plate for being symmetricly set on the engaging item right above liner including two and being adapted to the engaging item;
The engaging item includes Cross slat and the support column for being connected to Cross slat bottom surface;The Cross slat side is provided with engaging Slot;The connecting hole being arranged on the support column and liner, which is adapted to, to be plugged together;
The fastening plate includes the convex block and intermediate plate of two sides;The convex block and engaging slot sliding adaptation;The intermediate plate Bottom surface is provided with reagent bottle card slot.
4. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 3, it is characterised in that: institute It states and is provided with two limited blocks on box cover, the limited block lid plugs together the transverse direction for limiting the fastening plate in fastening plate two sides when closing Displacement.
5. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 4, it is characterised in that: institute It states and is additionally provided with pressing plate on box cover, be arranged between limited block, lid presses the fastening plate when lid closes.
6. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 5, it is characterised in that: institute It states limited block and is set as rectangular or arc.
7. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 5, it is characterised in that: institute It states pressing plate and is set as rectangular.
8. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 5, it is characterised in that: institute Stating liner is paper liner.
9. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 2, it is characterised in that: institute Stating reagent in uric acid reagent bottle I is sodium carbonate liquor, and reagent is Salkowski's solution, reagent in ammonium reagent bottle in uric acid reagent bottle II For Nessler's reagent, reagent is ethanol solution of ninhydrin in cystine reagent bottle, and reagent is that ammonium molybdate-nitric acid is molten in Phosphoric Acid bottle Liquid, the interior examination of hydrochloric acid reagent bottle is is hydrochloric acid solution, and reagent is sodium acetate solution in oxalic acid reagent bottle, and reagent is in calmagite bottle Sodium hydroxide solution, reagent is azo violet in azoviolet bottle.
10. dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit according to claim 2, it is characterised in that: Porcelain drop plate in six hole is provided with two rows of each three recessed grooves for waiting gaps;The teat glass specification is 5ml, quantity four It is a.
CN201920438230.4U 2019-04-02 2019-04-02 A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit Active CN209727957U (en)

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Application Number Priority Date Filing Date Title
CN201920438230.4U CN209727957U (en) 2019-04-02 2019-04-02 A kind of dog cat calculi in urinary system ingredient fast qualitative analysis utilizing kit

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