CN209707517U - A kind of platelet typing kit convenient for storage - Google Patents

Abstract

The utility model discloses a kind of platelet typing kits convenient for storage, bottle is added dropwise including low ionic medium, bottle is added dropwise in indicator cells, bottle is added dropwise in negative control sera, bottle and reaction component is added dropwise in positive control serum, the platelet typing kit convenient for storage further includes box body, box cover and internal partition, the box body internal layer, which stacks, is equipped with reactive multilayer component, multiple reaction micropores are provided on the reaction component, the position that the bottom surface of the reaction component corresponds to reaction micropore is provided with closure plug, the closure plug is for blocking the reaction micropore on lower layer's reaction component, closure corresponding with the reaction position of micropore is also equipped on the lower surface of the internal partition to fill in, the reaction micropore on reaction component for blocking top layer.This kind of kit can be stored simultaneously and largely have already passed through the processed reaction microplate of packet, stable storing, need using when need not wait coating platelet-specific antibody, shorten detection time.

Description

A kind of platelet typing kit convenient for storage

Technical field

The utility model belongs to biological detection reagent kit technical field, is related to a kind of platelet typing kit, specially A kind of platelet typing kit convenient for storage.

Background technique

Platelet transfusion is widely applied as a kind of effective treatment means in clinic.But due to long-term transfusion, The influence of drug therapy and disease itself causes patient's body to be easy to produce the allo-antibody for platelet antigen, these are anti- After body acts on human body platelet, purple defect etc. after Inefficacy of Platelets Transfusion disease, blood transfusion often can lead to, to jeopardize the life of patient Safety.It is provided in consideration of it, country defends planning commission in " clinical blood transfusion technical specification ", before platelet transfusion, it is necessary to carry out handing over adopted distribution type Experiment, to promote the blood transfusion quality of patient, guarantees the life security of patient.

The method of blood platelet crossmatch successively experienced blood platelet immunofluorescence experiment (PIFT), flow cytometer intersects The stages such as distribution type (FCM), micro-column gel distribution type.Above-mentioned matching method, which needs to realize by special installation, to be detected, and detection is not simple enough Just, quickly, and sensitivity is poor.Solid agglutination method (SPRCA) is that plate is added in blood platelet using packet processed lath has been done Centrifugation forms blood platelet molecular monolayer in item, and patients serum is then added, and after incubation and washing, instruction red blood cell is added, And interpretation result.The method sensitivity is good, high specificity, and it is easy, quickly, do not need special installation and can be completed, be current blood The mainstream technology in platelet distribution type field.

Platelet typing kit is the tool that solid agglutination method (SPRCA) must use, and is examined in some hospitals or blood Measured center requires largely to use platelet typing kit, but general platelet typing kit exists to increase blood platelet Coating rate on microplate requires to coat one layer of coating matrix on microplate in advance, but coats coating matrix and be also required to the time, If be coated with again when needing using platelet typing kit, entire detection time will necessarily be extended, but if thing First a large amount of microplate is coated in advance, then these will become a problem by the storage of coated microplate in advance, because This develop a kind of platelet typing kit convenient for storage very it is necessary to.

Utility model content

The utility model discloses a kind of platelet typing kit convenient for storage, this kind of kit can be stored simultaneously Largely have already passed through the processed reaction microplate of packet, stable storing, need using when coating blood-platelet specific need not be waited anti- Body shortens detection time.

In order to achieve the above object, the utility model adopts the following technical solution:

A kind of platelet typing kit convenient for storage, including bottle is added dropwise in low ionic medium, bottle, yin is added dropwise in indicator cells Property bottle is added dropwise in control serum, bottle and reaction component is added dropwise in positive control serum, the platelet typing kit convenient for storage It further include box body, box cover and internal partition, the box body internal layer, which stacks, is equipped with reactive multilayer component, is provided on the reaction component Multiple reaction micropores, the position that the bottom surface of the reaction component corresponds to reaction micropore are provided with closure plug, and the closure plug is used In blocking the reaction micropore on lower layer's reaction component, it is also equipped on the lower surface of the internal partition and the position pair of reacting micropore The closure plug answered, the reaction micropore on reaction component for blocking top layer.

Further, the reaction component includes outline border and at least two microplates, and the upper side frame of the outline border is under The upper surface of frame is provided with several mounting grooves, and the upper and lower ends of the microplate are engaged cooperation with mounting groove, so that instead Microplate is answered to be removably attached on outline border by mounting groove, it is equally spaced on each described reaction microplate to be equipped with several instead Answer micropore.

Further, the outline border is by upper and lower two the first frames disposed in parallel and left and right two disposed in parallel second Frame splicing fixation forms.

Further, the mounting groove is equally spaced in the upper surface of the first frame, each the first frame it is upper The quantity of mounting groove on surface is 12, and the quantity of the reaction microplate is 12, on reaction microplate described in each etc. Compartment of terrain is equipped with 8 reaction micropores.

Further, the material of the reaction microplate is polystyrene.

Further, label layer is additionally provided on the outline border.

Further, the reaction micropore is in U-shape groove-like.

Further, the space on the internal partition top is used to place low ionic medium dropwise addition bottle, bottle is added dropwise in indicator cells, Bottle is added dropwise in negative control sera and bottle is added dropwise in positive control serum.

Further, the upper surface of the internal partition is provided with spongy layer, is provided on the spongy layer low for placing Bottle is added dropwise in ionic medium, bottle is added dropwise in indicator cells, bottle is added dropwise in negative control sera and the groove of bottle is added dropwise in positive control serum.

Further, the box cover is fastened on the oral area of box body, is formed and is sealed to tray interior.

Compared with prior art, the utility model has the following beneficial effects:

(1) the platelet typing kit convenient for storage of the utility model, further includes box body, box cover and internal partition, box It can be laminated in vivo and place multiple reaction components, can be placed on multiple in box body by coated reaction component, not only save About parking space, and since the position that the lower surface of each reaction component corresponds to reaction micropore is provided with closure plug, When placement is laminated, a plugging action can be formed to the oral area for the reaction micropore for being placed on lower layer by blocking plug, therefore deposited Dust is not had during putting to enter in reaction micropore, the air reacted in micropore is also seldom, reduces the substance pair in air The influence of the coating substance in micropore is reacted, will not cause to be coated with substance failure and influence detection effect due to storage.Therefore, should Kind of kit can be stored simultaneously and largely have already passed through the processed reaction microplate of packet, stable storing, need using when need not wait Platelet-specific antibody to be coated, shortens detection time.

(2) the platelet typing kit convenient for storage of the utility model, two the first frames up and down of the kit On be equipped with mounting groove, react the upper and lower ends of microplate and mounting groove be engaged cooperation so that reaction microplate be removably mounted on In mounting groove.The kit platelet typing method of the utility model is simple and quick, and does not need special installation, can single part inspection Survey, can also scale detection, to reduce testing cost, provide convenience for clinical detection, have preferable clinical value.

Detailed description of the invention

Fig. 1 be the utility model proposes convenient for storage platelet typing kit diagrammatic cross-section；

Fig. 2 is the bottom view of reaction component；

Fig. 3 is the top view of reaction component.

Description of symbols: 1- box body, 2- box cover, 3- internal partition, 4- react micropore, and 5-, which is blocked, to be filled in, 6- spongy layer, outside 7- Frame, the first frame of 701-, the second frame of 702-, 8- microplate, 9- mounting groove.

Specific embodiment

The present invention will be further described for embodiment with reference to the accompanying drawing.

A kind of platelet typing kit convenient for storage, including bottle is added dropwise in low ionic medium, bottle, yin is added dropwise in indicator cells Property bottle is added dropwise in control serum, bottle and reaction component is added dropwise in positive control serum.

Referring to attached drawing 1 and attached drawing 2, this kind further includes box body 1, box cover 2 and interior convenient for the platelet typing kit of storage Partition 3, box cover 2 are fastened on the oral area of box body 1, seal to being formed inside box body 1.1 internal layer of box body, which stacks, is equipped with reactive multilayer group Part can disposably store multiple reaction components, take up little area；It is provided with multiple reaction micropores 4 on reaction component, reacts micropore 4 Endoperidium platelet-specific antibody, such as monoclonal platelet antibody；In order to guarantee will not to pollute brokenly during storage The bad reaction micropore 4 being coated, the position that the bottom surface of reaction component corresponds to reaction micropore 4 are provided with closure plug 5, block plug 5 can be elastic rubber plug, block plug 5 for blocking reaction micropore 4 on lower layer's reaction component, on the lower surface of internal partition 3 It is also equipped with and fills in 5 with corresponding closures in the reaction position of micropore 4, the reaction micropore 4 on reaction component for blocking top layer, Due to the presence of internal partition 3, the number of plies for the reaction component stored in box body 1 can be arranged according to demand, each layer of reaction group Reaction micropore 4 on part can be blocked plug 5 and be blocked.

For placing, bottle is added dropwise in low ionic medium, bottle, negative control sera is added dropwise in indicator cells in the space on 3 top of internal partition Bottle is added dropwise and bottle is added dropwise in positive control serum.Preferably, the upper surface of internal partition 3 is provided with spongy layer 6, is arranged on spongy layer 6 Have and bottle, indicator cells dropwise addition bottle, negative control sera dropwise addition bottle and positive control serum drop is added dropwise for placing low ionic medium Add the groove (not shown) of bottle.

It is further used as being preferably carried out mode, referring to attached drawing 3, reaction component includes outline border 7 and at least two microwell plates Item 8, the material for reacting microplate is polystyrene, is not involved in detection reaction.Outline border 7 is by upper and lower two the first frames disposed in parallel 701 and the second frames 702 disposed in parallel splicing fixation of left and right two form.The upper side frame of outline border 7 and the upper surface of lower frame Several mounting grooves 9 are provided with, are additionally provided with label layer on outline border 7, for identifying each reaction micropore 4.Above and below microplate 8 Both ends are engaged cooperation with mounting groove 9, so that reaction microplate is removably attached on outline border 7 by mounting groove 9, each reaction Equally spaced on microplate to be equipped with several reaction micropores 4, reaction micropore 4 is in U-shape groove-like.Preferably, mounting groove 9 is equally spaced The upper surface of first frame 701 is set, the quantity of the mounting groove 9 on the upper surface of each the first frame 701 is 12, The quantity for reacting microplate is 12, and number 1~12 is also marked on at least one the first frames 701, corresponds respectively to each peace The position of tankage 9；Each, which is reacted, is equally spaced equipped with 8 reaction micropores 4 on microplate, on at least one the second frames 702 and also Equipped with alphabetical A~H, for marking the coordinate of each reaction micropore 4.

The foregoing is merely the better embodiment of the utility model, the utility model is not limited to above-mentioned embodiment party Formula, there may be the structural modifications that part is small in implementation process, if various changes or modifications to the utility model are not The spirit and scope of the utility model are detached from, and are belonged within the scope of the claims and equivalents of the utility model, then originally Utility model is also intended to encompass these modification and variations.

Claims (10)

1. a kind of platelet typing kit convenient for storage, including bottle is added dropwise in low ionic medium, bottle, feminine gender is added dropwise in indicator cells Bottle is added dropwise in control serum, bottle and reaction component is added dropwise in positive control serum, which is characterized in that the blood platelet convenient for storage is matched Type kit further includes box body, box cover and internal partition, and the box body internal layer, which stacks, is equipped with reactive multilayer component, the reaction component On be provided with multiple reaction micropores, the position that the bottom surface of the reaction component corresponds to reaction micropore is provided with closure plug, described Plug is blocked for blocking the reaction micropore on lower layer's reaction component, is also equipped on the lower surface of the internal partition and reacts micropore The corresponding closure in position plug, the reaction micropore on reaction component for blocking top layer.

2. the platelet typing kit according to claim 1 convenient for storage, which is characterized in that the reaction component packet Outline border and at least two microplates are included, the upper side frame of the outline border and the upper surface of lower frame are provided with several mounting grooves, The upper and lower ends of the microplate are engaged cooperation with mounting groove, so that reaction microplate is removably attached to outside by mounting groove It is equally spaced on each described reaction microplate to be equipped with several reaction micropores on frame.

3. the platelet typing kit according to claim 2 convenient for storage, which is characterized in that the outline border is by upper and lower The the second frame splicing fixation disposed in parallel of two the first frames disposed in parallel and left and right two forms.

4. the platelet typing kit according to claim 3 convenient for storage, which is characterized in that between described mounting groove etc. The upper surface of the first frame is set every ground, the quantity of the mounting groove on the upper surface of each the first frame is 12, institute The quantity for stating reaction microplate is 12, is equally spaced equipped with 8 reaction micropores on reaction microplate described in each.

5. the platelet typing kit according to claim 4 convenient for storage, which is characterized in that the reaction microplate Material is polystyrene.

6. convenient for the platelet typing kit of storage according to any one of claim 2~5, which is characterized in that institute It states and is additionally provided with label layer on outline border.

7. the platelet typing kit according to any one of claims 1 to 5 convenient for storage, which is characterized in that institute Reaction micropore is stated to be in U-shape groove-like.

8. the platelet typing kit according to any one of claims 1 to 5 convenient for storage, which is characterized in that institute Bottle is added dropwise for placing low ionic medium in the space for stating internal partition top, bottle is added dropwise in indicator cells, bottle is added dropwise in negative control sera Bottle is added dropwise with positive control serum.

9. it is according to claim 8 convenient for storage platelet typing kit, which is characterized in that the internal partition it is upper Surface is provided with spongy layer, is provided on the spongy layer and bottle, indicator cells dropwise addition bottle, yin is added dropwise for placing low ionic medium Property control serum the groove that bottle is added dropwise in bottle and positive control serum is added dropwise.

10. the platelet typing kit according to claim 9 convenient for storage, which is characterized in that the box cover fastens In the oral area of box body, tray interior is formed and is sealed.