CN209685806U - Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs - Google Patents
Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs Download PDFInfo
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- CN209685806U CN209685806U CN201920163796.0U CN201920163796U CN209685806U CN 209685806 U CN209685806 U CN 209685806U CN 201920163796 U CN201920163796 U CN 201920163796U CN 209685806 U CN209685806 U CN 209685806U
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Abstract
The utility model discloses a kind of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs.The three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE includes the bottom plate, orifice plate and lid successively cooperated；Bottom plate is a groove body；Bottom plate and orifice plate are cooperated by groove；The side wall of bottom plate forms the groove；The side wall that the upper and lower surface of orifice plate extends can be inserted into groove.The utility model cell culture plate structure has three-decker, i.e. in one layer of floor structure of orifice plate following settings, the bottom plate is a groove body, it can be filled in its cavity moisturizer (such as water, buffer), it can play the role of moisturizing cell culture plate, the volatilization for slowing down the reagents such as the culture medium in cell cultivation process, is more advantageous to cell culture.Moreover, the side wall that the upper and lower surface of the orifice plate extends can be inserted into the groove, i.e., the obverse and reverse of the described orifice plate can be buckled into the groove of the bottom plate, so as to make the face-up or reverse side of the orifice plate upward.
Description
Technical field
The utility model relates to a kind of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs.
Background technique
Existing cell culture plate is double-layer structure, is mainly made of porous mainboard and lid, so that operating micro liquid
When body, due to lacking effectively moist keeping measures, it is easy because liquid evaporation causes experiment inaccurate, unsuccessfully etc..Existing 96 hole is more
There is diamond shape gap that the moistening effect that moisturizer reaches certain can be added between Kong Yukong on the mainboard of hole, but due to each gap
Be it is independent, be all added that moisturizer are more time-consuming and laborious, and orifice plate is generally homochromatic or transparent toward the gap between 96 holes,
The more difficult gap being clear that between micropore and micropore is easy for moisturizer not to be added in micropore.And the hole of 384 orifice plates with
Gap is not present between hole, therefore more difficult when micro liquid is manually operated on 384 orifice plates.In addition, Chinese invention is special
Benefit application (201310359407.9) discloses a kind of biological reaction apparatus, is a kind of novel three-dimensional cell culture plate, special
Point is that its mainboard is transparent micropore, therefore is only capable of preventing by lid using existing cell culture plate double-layer structure
Only pollutant enters above mainboard and liquid is inhibited to volatilize from above, but due to mainboard be it is transparent, pollutant also may be used
With from bottom surface enter and liquid can also volatilize from ground.Therefore either traditional real bottom micropore and novel transparent micropore
It requires three completely new confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs of one kind porous mainboard is clipped in the middle, provides moistening effect under from bottom
Layer protection, and lid provides the loss that can inhibit the liquid up to volatilize while the protection of upper layer.
Utility model content
The purpose of the utility model is to provide a kind of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs, have a bottom plate, the bottom plate
Cavity in can be filled moisturizer (such as water, buffer), moisturizing cell culture plate can be played the role of, slow down cell training
The volatilization of the reagents such as the culture medium during supporting, is more advantageous to cell culture.
Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE provided by the utility model, it includes the bottom plate, orifice plate and lid successively cooperated
Son；
The bottom plate is a groove body, and moisturizer can be filled in the groove body；
The bottom plate and the orifice plate are cooperated by groove；
The side wall of the bottom plate forms the groove, and the side wall of the bottom plate is inserted into the groove, to realize institute
State the cooperation of bottom plate Yu the orifice plate.
Specifically, the side wall that the upper and lower surface of the orifice plate extends can be inserted into the groove, i.e., the described orifice plate
Obverse and reverse can be buckled into the groove of the bottom plate, so as to make the face-up or reverse side of the orifice plate
Upward；For edition type of transparent micropore, especially 384, since the hole location arrangement of the microwell plate of 384 editions types is intensive, and hole
It is deeper, it is more difficult to liquid is precisely added to hole location center, is often easy for be even suspended on hole wall the corner that liquid is added to hole,
Therefore by the utility model, i.e., orifice plate is bottom-up, transparent micropore bottom may be implemented in orifice plate left-hand thread does not have
The interference of hole wall facilitates user that can see hole location center clearly, realizes and liquid is added dropwise in center, and will not wall built-up.
In the three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs, it is equipped with bracket in the cavity of the bottom plate, such as can be cross branch
Frame can pollute into the orifice plate to avoid the liquid splash in the bottom plate
In the three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs, the orifice plate includes the mainboard in outline border and the outline border.
The mainboard is equipped with several cell culture wells, and property can be round, rectangular, polygon, irregular shape；Length and width
Full-size≤3.5mm, thickness are less than or equal to 0.1~20mm；The arrangement of the cell culture well can be according to ANSI SLAS1-
2004 (R2012) international standard cell culture plate specifications, i.e., 3 × 26 orifice plates, 4 × 3 12 orifice plates, 6 × 4 24 orifice plates, 8
× 6 48 orifice plates, 12 × 8 96 orifice plates, 24 × 16 384 orifice plates or 48 × 32 1536 orifice plates etc..
In the three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs, the bottom plate, the orifice plate and the lid, which are equipped with, distinguishes that position is cut
Angle, with facilitate user identify the orifice plate hole location arrangement sequence (such as it is considered that ranking sequence can from no unfilled corner to
Having unfilled corner is positive sequence).
Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE of the utility model has the advantages that
The utility model cell culture plate structure has three-decker, i.e., in one layer of floor structure of orifice plate following settings,
The bottom plate is a groove body, can fill moisturizer (such as water, buffer) in its cavity, can play moisturizing cell culture well
The effect of plate slows down the volatilization of the reagents such as the culture medium in cell cultivation process, is more advantageous to cell culture.Moreover, the hole
The side wall that the upper and lower surface of plate extends can be inserted into the groove, i.e., the obverse and reverse of the described orifice plate can buckle into
In the groove of the bottom plate, so as to make the face-up or reverse side of the orifice plate upward.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE of the utility model.
Sectional view when Fig. 2 is three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE insole board of the utility model and orifice plate cooperation.
Fig. 3 is the structural schematic diagram of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE insole board of the utility model.
It is respectively marked in figure as follows:
A corner cut, 6 cross brackets, 7 groove bodies are distinguished in 1 bottom plate, 2 orifice plates, 3 lids, 4 grooves, 5.
Specific embodiment
The utility model is described further with reference to the accompanying drawing, but the utility model is not limited to following implementation
Example.
As shown in Figure 1, being the structural schematic diagram (when unmated) of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE of the utility model, it is wrapped
Include the bottom plate 1, orifice plate 2 and lid 3 successively cooperated.Wherein, bottom plate 1 is a groove body 7, and moisturizer can be filled (such as in the groove body
Water, buffer etc.), moisturizing cell culture plate can be played the role of.The side wall of bottom plate 1 is a groove 4, the side wall of orifice plate 2
It is inserted into groove 4, to realize the cooperation of bottom plate 1 Yu orifice plate 2, as shown in Figure 2.Bottom plate 1, orifice plate 2 and lid 3 are equipped with
Distinguish a corner cut 5, (for example it is considered that ranking sequence can be from intact to facilitate user to identify the sequence of hole location arrangement of orifice plate 2
Angle to have unfilled corner be positive sequence).In addition, the side wall that the upper and lower surface of orifice plate 2 extends can be inserted into groove 4, i.e., orifice plate 2 is being just
Face and reverse side can be buckled into the groove 4 of bottom plate 1, so as to make the face-up or reverse side of orifice plate upward.
Furthermore it is also possible to multiple cross brackets 6 are set in the cavity of bottom plate 1, it, can be to avoid in bottom plate 1 if Fig. 3 shows
Liquid splash polluted into the orifice plate.
In the utility model, orifice plate 2 may include the mainboard (not shown) in outline border (not shown) and outline border, on mainboard
Equipped with several cell culture wells, property can be round, rectangular, polygon, irregular shape；Length and width full-size≤3.5mm is thick
Degree is less than or equal to 0.1~20mm；The arrangement of the cell culture well can be according to ANSI SLAS1-2004 (R2012) international standard
Cell culture plate specification, i.e., 3 × 26 orifice plates, 4 × 3 12 orifice plates, 6 × 4 24 orifice plates, 8 × 6 48 orifice plates, 12 × 8
96 orifice plates, 24 × 16 384 orifice plates or 48 × 32 1536 orifice plates etc..
When using three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE of the utility model, suitable guarantor is filled in the cavity of bottom plate 1 first
Orifice plate 2, is then buckled on bottom plate 1, the side wall of orifice plate 2 is specifically inserted into the recessed of bottom plate 2 by humectant (such as water, buffer)
In slot 4, the cell and culture medium that need to cultivate then are added into cell culture well, closes the lid and is cultivated after 3.
Claims (5)
1. a kind of three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs, it is characterised in that: the three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE includes successively matching
Bottom plate, orifice plate and the lid of conjunction；
The bottom plate is a groove body；
The bottom plate and the orifice plate are cooperated by groove；
The side wall of the bottom plate forms the groove.
2. three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE according to claim 1, it is characterised in that: the upper and lower surface of the orifice plate is prolonged
The side wall stretched can be inserted into the groove.
3. three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE according to claim 1 or 2, it is characterised in that: in the cavity of the bottom plate
Equipped with bracket.
4. three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE according to claim 1 or 2, it is characterised in that: the orifice plate includes outline border
With the mainboard in the outline border.
5. three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTURE according to claim 1 or 2, it is characterised in that: the bottom plate, the orifice plate
It is equipped with the lid and distinguishes a corner cut.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920163796.0U CN209685806U (en) | 2019-01-30 | 2019-01-30 | Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920163796.0U CN209685806U (en) | 2019-01-30 | 2019-01-30 | Three confluent monolayer cells culture AND DEWATERING FOR ORIFICE STRUCTUREs |
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| Publication Number | Publication Date |
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| CN209685806U true CN209685806U (en) | 2019-11-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111349546A (en) * | 2020-03-18 | 2020-06-30 | 江苏支点生物科技有限公司 | Cell-free protein synthesis method and porous plate |
| CN113388514A (en) * | 2020-03-12 | 2021-09-14 | 株式会社理光 | Drying inhibition device and cell culture kit |
-
2019
- 2019-01-30 CN CN201920163796.0U patent/CN209685806U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388514A (en) * | 2020-03-12 | 2021-09-14 | 株式会社理光 | Drying inhibition device and cell culture kit |
| CN111349546A (en) * | 2020-03-18 | 2020-06-30 | 江苏支点生物科技有限公司 | Cell-free protein synthesis method and porous plate |
| CN111349546B (en) * | 2020-03-18 | 2020-09-15 | 江苏支点生物科技有限公司 | Cell-free protein synthesis method and porous plate |
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