CN209669259U - A kind of canine coronavirus quick detection kit - Google Patents
A kind of canine coronavirus quick detection kit Download PDFInfo
- Publication number
- CN209669259U CN209669259U CN201920129822.8U CN201920129822U CN209669259U CN 209669259 U CN209669259 U CN 209669259U CN 201920129822 U CN201920129822 U CN 201920129822U CN 209669259 U CN209669259 U CN 209669259U
- Authority
- CN
- China
- Prior art keywords
- micro
- fluidic chip
- chamber
- reagent
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model provides a kind of canine coronavirus quick detection kit, including packing box, partition, micro-fluidic chip A, micro-fluidic chip B, reagent pedestal tube, Reagent Tube, Rule plug, specification, packing box is divided into the chamber of left, center, right three by partition, and it is several to be placed in parallel micro-fluidic chip A along the vertical direction in first chamber；It is several that micro-fluidic chip B is placed in parallel in second chamber along the vertical direction；Well, fluid channel, DNA separation chamber, waste liquid chamber, triangle vavle, PCR reaction zone and/or fluorescence detection room are equipped on micro-fluidic chip；Third chamber is divided into upper layer and lower layer by reagent pedestal tube, and the Reagent Tube for holding DNA separation magnetic bead is placed in the hole of reagent pedestal tube, and Rule plug is placed in reagent pedestal tube lower part.The utility model can a step realize the highly sensitive, quick of canine coronavirus, fluorescence quantitative PCR detection, it is simple and efficient to handle.
Description
Technical field
The utility model relates to animal epidemic detection technique fields, and in particular to a kind of canine coronavirus quick detection reagent
Box.
Background technique
Canine coronavirus disease (ccv) belongs to coronaviridae coronavirus genus member.Virus has coronavirus really general
Morphological feature, rounded or oval, 80~120nm of major diameter, wide diameter are 75~80nm, have cyst membrane, there is petal-shaped on cyst membrane surface
Fibre is prominent, is about 20nm, freeze thawing easily falls off, and is infectivity.Nucleocapsid is in the shape of a spiral.Virogene type is single-stranded RNA.Virus
Buoyant density in CsCL is 1.15~1.16 grams/cc.Virus is to chloroform, ether, deoxycholic acid salt density value.To heat
It is sensitive.It can be inactivated with formaldehyde, ultraviolet light.There is resistance to trypsase and acid.Virus is survived 6~9 days in excrement.
Canine coronavirus incubation period 1~5 day, clinical features were different.It is mainly shown as vomiting and diarrhea, serious disease dog
Lassitude is in drowsiness shape, poor appetite or abolish, and majority is without Temperature changing.Thirsty, asoscope drying, vomiting, last from days go out
Existing diarrhea.Excrement is in athero- or water sample, red or crineous or yellow green, stench, is mixed with mucus or a small amount of blood.Leucocyte
It counts normally, the course of disease 7~10 days, dead in 1~2 day after the onset of some sick dog especially puppies, adult dogs are seldom dead.At present originally
Disease there is no effective vaccine prevention and specific short.Therefore, it can be diagnosed to be the virus infection as early as possible in pet epidemic prevention and control
Seem more important.
Currently, canine coronavirus detection main method has virus purification, experimental animal infection, inclusion body inspection, Electronic Speculum to see
It examines, the examination of the neutralization test of CAV, enzyme-linked immunosorbent assay (ELISA), colloid gold test paper, enzymic change foci decoration method, immunofluorescence
It tests, the methods of chain polymerization enzyme reaction.
But these methods are each defective, Virus Isolation and neutralization test process are cumbersome, take long time；Electron microscopy
It is difficult in base's popularization and application；ELISA only can qualitative detection virus, and needed in operating process for a long time be incubated for and rinse, consumption
When it is longer, these detection methods are difficult in veterinary clinic field application；Though colloid gold test paper can be realized quick detection, can not
Realize the quantitative detection of virus.
Utility model content
The technical problem to be solved by the utility model is to how quick and quantitative detection canine coronavirus, optimize beast
Cure clinical sites application.
To solve the above problems, inventor is based on quantitative fluorescent PCR and microflow control technique principle, it is coronal to design a kind of dog
Virus rapid detecting kit can operate rapid and convenient with the rapid quantitative detection of realization canine coronavirus in the short time.
The utility model provides a kind of canine coronavirus quick detection kit, including packing box, partition, micro-fluidic core
Piece A, micro-fluidic chip B, reagent pedestal tube, Reagent Tube, Rule plug, specification, it is characterised in that: the partition is by packing box area
It is divided into the chamber of left, center, right three, it is several to be placed in parallel micro-fluidic chip A in first chamber along the vertical direction, the micro-fluidic chip
A is rectangular configuration, and micro-fluidic chip A is equipped with 2 sample holes, 1 DNA separation chamber, 1 triangle vavle, 1 waste liquid chamber, 1
Mixing chamber, 1 S-shaped fluid channel PCR reaction zone, 1 fluorescence detection room and the fluid channel for connecting each room；Along vertical in second chamber
It is several that direction is placed in parallel micro-fluidic chip B；The micro-fluidic chip B be rectangular configuration, micro-fluidic chip B be equipped with 2 into
Sample hole, 1 DNA separation chamber, 1 triangle vavle, 1 waste liquid chamber, the fluid channel of 1 PCR reaction chamber and each room of connection；Third chamber
1 reagent pedestal tube of interior setting, the Reagent Tube for holding DNA separation magnetic bead is placed in the hole of reagent pedestal tube, under reagent pedestal tube
Place Rule plug in side.
The micro-fluidic chip material can be selected from glass, PMMA, PC, COC or COP.The specification of micro-fluidic chip A is 102
×76 mm；The specification of micro-fluidic chip B is preferably 76 × 26mm.Micro-fluidic reaction chamber bottom surface, fluid channel inner surface can carry out parent
Water or hydrophobic treatment.
The utility model has an advantage that compared with prior art
1, the utility model being capable of step realization dog DNA separation and the highly sensitive, quick of coronavirus, quantitative detection.
2, the utility model operating process is simple and efficient, different detection reagents of arranging in pairs or groups, and it is fixed can to carry out single fluorescence respectively
Amount analysis, or multiple fluorescence quantitative PCR analysis is carried out simultaneously.
3, the utility model provides two different micro-fluidic chips, selects according to demand for user, easy spirit
It is living.
Detailed description of the invention
The structural schematic diagram of Fig. 1 the utility model canine coronavirus quick detection kit, marked in the figure: 1- packing box,
2- partition, 3- micro-fluidic chip A, 4- micro-fluidic chip B, 5- reagent pedestal tube, 6- Reagent Tube, 7- Rule plug, 8- specification.
The structural schematic diagram of micro-fluidic chip A in Fig. 2 the utility model canine coronavirus quick detection kit, in figure
Label: 3- micro-fluidic chip, 31- sample holes, the separation chamber 32-DNA, 33- triangle vavle, 34- waste liquid chamber, 35- mixing chamber, 36-
PCR reaction zone, 37- fluorescence detection room.
The structural schematic diagram of micro-fluidic chip B in Fig. 3 the utility model canine coronavirus quick detection kit, in figure
Label: 4- micro-fluidic chip, 41- sample holes, the separation chamber 42-DNA, 43- triangle vavle, 44- waste liquid chamber, 45-PCR reaction chamber.
Specific embodiment
The utility model is further described with reference to the accompanying drawings and embodiments.It should be noted that in embodiment not in detail
The normal condition and method of explanation, the experiment condition and method routinely used according to fields personnel carry out.
Embodiment:
As shown in Figure 1-Figure 3, a kind of canine coronavirus quick detection kit, including packing box (1), partition (2), miniflow
Control chip A3, micro-fluidic chip B4, reagent pedestal tube 5, Reagent Tube 6, Rule plug 7, specification 8；The partition (2) is by packing box
(1) chamber of left, center, right three is divided into, it is several to be placed in parallel micro-fluidic chip A3 in first chamber along the vertical direction, the miniflow
Control chip A3 is rectangular configuration, and micro-fluidic chip A3 is equipped with 2 triangle vavles 33,1 of DNA separation chamber 32,1 of sample holes 31,1
36, the 1 fluorescence detection rooms 37 of the S-shaped fluid channel PCR of mixing chamber 35,1 reaction zone of a waste liquid chamber 34,1 and each room of connection it is micro-
Runner；It is several that micro-fluidic chip B4 is placed in parallel in second chamber along the vertical direction；The micro-fluidic chip B4 is rectangular configuration,
It is anti-that micro-fluidic chip B4 is equipped with 2 waste liquid chamber 44,1 of triangle vavle 43,1 of DNA separation chamber 42,1 of sample holes 41,1 PCR
It answers room 45 and connects the fluid channel of each room；1 reagent pedestal tube 5 is set in third chamber, holds the Reagent Tube of DNA separation magnetic bead
6 are placed in the hole of reagent pedestal tube 5, and it is several that Rule plug 7 is placed below reagent pedestal tube 5.The specification of the micro-fluidic chip A3
For 102 × 76mm；The specification of micro-fluidic chip B4 is 76 × 26mm.
The material of the micro-fluidic chip A3 and micro-fluidic chip B (4) can be selected from glass, PMMA, PC, COC or COP.
The application method of canine coronavirus quick detection kit described in the utility model:
1, micro-fluidic chip A:
1) protective film at sample holes A (sample holes being directly connected to DNA separation chamber) is torn, DNA is separated into magnetic bead 2-
5 μ l inject DNA separation chamber；
2) samples to be tested such as animal blood serum, saliva, urine 50-100 μ l is injected into DNA separation chamber, reaction chamber from sample holes A
Interior air surveys through-hole discharge from triangle vavle, avoids blowing out bubble in reaction chamber as far as possible, closes sample introduction using Rule plug or plug
Hole, control triangle vavle close the through-hole for connecting DNA separation chamber；
3) micro-fluidic chip is placed on magnetic force blending instrument, opens switch, drive DNA to separate magnetic bead in sample by magnetic force
In flow repeatedly, DNA is adsorbed on magnetic bead, this step about 5-10 minutes；
4) DNA separation chamber is connected to by control triangle vavle with waste liquid chamber, buffer is injected from sample holes A, by remaining sample
It is discharged into waste liquid chamber, closing the interior through-hole for connecting DNA separation chamber of triangle vavle, (magnetic force blending instrument is always on during this, guarantees to inhale
The magnetic bead of attached DNA will not be flushed away)；
5) magnetic bead 30-60s is rinsed using buffer, DNA separation chamber is connected to waste liquid chamber, waste liquid is arranged by control triangle vavle
Enter waste liquid chamber；It repeats this flushing process 1-3 times, waste liquid is all discharged using air pump；
6) DNA eluent is injected from sample holes A, it is identical as above-mentioned rinsing step, DNA is eluted；
7) DNA separation chamber is connected to by control triangle vavle with mixing chamber, is injected the DNA of elution in mixing chamber using air pump,
PCR reaction reagent mixture is injected into mixing chamber from sample holes B (sample holes being directly connected to mixing chamber), can be shaken manually mixed
Close, can also preset magnetic rotor in chip, mixed using magnetic force blending instrument；
8) mixing chamber is connected to by control triangle vavle with PCR reaction zone, micro- in reaction zone using air pump control PCR reaction system
Each heating module is flowed through in runner, completes DNA unwinding, is annealed, and is extended, it is final to import fluorescence detection room.Air pump can control liquid
The flowing velocity of body different zones in fluid channel guarantees the accurate completion of PCR program；Different heating modules is arranged different
Temperature, corresponding PCR reaction process demand.
9) by fluorescence detection room, instrument readings calculate viral load according to standard curve.
2, micro-fluidic chip B:
Step 1-6 is identical as micro-fluidic chip A.
7, DNA separation chamber is connected to by control triangle vavle with PCR reaction chamber, is reacted the DNA injection PCR of elution using air pump
PCR reaction reagent mixture is injected PCR reaction chamber from sample holes B (sample holes being directly connected to PCR reaction chamber) by interior,
Mixing can be shaken manually, can also preset magnetic rotor in chip, mixed using magnetic force blending instrument；
8, PCR program is set, control PCR instrument heating module corresponds to PCR reaction process demand.
9, single or multi-fluorescence is monitored by PCR reaction chamber, instrument readings calculate viral load according to standard curve.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection scope within.
Claims (3)
1. a kind of canine coronavirus quick detection kit, including packing box (1), partition (2), micro-fluidic chip A (3), miniflow
Control chip B (4), reagent pedestal tube (5), Reagent Tube (6), Rule plug (7), specification (8), it is characterised in that: the partition (2)
Packing box (1) is divided into the chamber of left, center, right three, be placed in parallel micro-fluidic chip A (3) along the vertical direction in first chamber if
Dry, the micro-fluidic chip A (3) is rectangular configuration, and micro-fluidic chip A (3) is equipped with 2 sample holes (31), 1 DNA separation
Room (32), 1 triangle vavle (33), 1 waste liquid chamber (34), 1 mixing chamber (35), 1 S-shaped fluid channel PCR reaction zone (36), 1
The fluid channel of a fluorescence detection room (37) and each room of connection；Micro-fluidic chip B (4) are placed in parallel in second chamber along the vertical direction
It is several；The micro-fluidic chip B (4) is rectangular configuration, and micro-fluidic chip B (4) is equipped with 2 sample holes (41), 1 DNA points
From room (42), the fluid channel of 1 triangle vavle (43), 1 waste liquid chamber (44), 1 PCR reaction chamber (45) and each room of connection；Third
1 reagent pedestal tube (5) is set in chamber, the Reagent Tube (6) for holding DNA separation magnetic bead is placed in the hole of reagent pedestal tube (5),
Rule plug (7) are placed below reagent pedestal tube (5).
2. canine coronavirus quick detection kit according to claim 1, which is characterized in that the micro-fluidic chip A
(3) specification is 102 × 76mm；The specification of micro-fluidic chip B (4) is 76 × 26mm.
3. canine coronavirus quick detection kit according to claim 1, which is characterized in that the micro-fluidic chip A
(3) and the material of micro-fluidic chip B (4) can be selected from glass, PMMA, PC, COC or COP.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920129822.8U CN209669259U (en) | 2019-01-25 | 2019-01-25 | A kind of canine coronavirus quick detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920129822.8U CN209669259U (en) | 2019-01-25 | 2019-01-25 | A kind of canine coronavirus quick detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209669259U true CN209669259U (en) | 2019-11-22 |
Family
ID=68567139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920129822.8U Expired - Fee Related CN209669259U (en) | 2019-01-25 | 2019-01-25 | A kind of canine coronavirus quick detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209669259U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115193358A (en) * | 2022-07-21 | 2022-10-18 | 扬州大学 | Test tube-shaped multifunctional microfluidic reaction device |
-
2019
- 2019-01-25 CN CN201920129822.8U patent/CN209669259U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115193358A (en) * | 2022-07-21 | 2022-10-18 | 扬州大学 | Test tube-shaped multifunctional microfluidic reaction device |
| CN115193358B (en) * | 2022-07-21 | 2024-05-28 | 扬州大学 | Test tube-shaped multifunctional microfluidic reaction device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107012067B (en) | A kind of high-throughput pairing captures micro-fluidic chip and its application of unicellular/individual particle | |
| US9468894B2 (en) | Microfluidic mixing and analytical apparatus | |
| CN103055967B (en) | A kind of preparation of simple to operate, low cost, multichannel micro-fluidic chemiluminescence paper chip and the application in detecting at the scene | |
| ES2298244T5 (en) | Method for the detection of influenza A / B viruses in saliva | |
| ES2461242T3 (en) | Magnetic material to collect and use magnetic particles | |
| CN107930710A (en) | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system and their application | |
| CN1234117A (en) | Use of anti-embryonic hemoglobin antibodies to identify fetal cells | |
| CN110496655A (en) | A kind of tumour cell detection chip based on microflow control technique | |
| CN101568636A (en) | Cartridge for conducting diagnostic assays | |
| CN207786624U (en) | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system | |
| CN207851087U (en) | A kind of canine virus bigeminy Quantitative detection micro-fluidic chip | |
| BRPI0615678A2 (en) | method to diagnose infections | |
| CN111458504A (en) | Kit for detecting IgG antibody against novel coronavirus SARS-COV-2 in sample | |
| CA3185631A1 (en) | Kits and methods for the enrichment and detection of rna viruses of the coronaviridae family | |
| CN109295183A (en) | A kind of method and system of quick detection sample of nucleic acid | |
| CN209669259U (en) | A kind of canine coronavirus quick detection kit | |
| CN103981285B (en) | A kind of detect the method for bovine viral diarrhea virus in aerosol | |
| CN104726606B (en) | A kind of enzyme-linked double cross method detection the pathogenic microorganism examination methods of PCR | |
| CN209669228U (en) | A kind of canine virus multiple fluorescence quantitative PCR detection micro-fluidic chip | |
| CN207793229U (en) | Molecule diagnoses micro-fluidic chip and molecule diagnoses micro-fluidic chip system | |
| Song et al. | Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip | |
| CN110023758A (en) | For separating equipment, system, method and the external member of analyte from body fluid sample | |
| Grimes et al. | Application of a microtiter cell-culture method to characterization of avian adenoviruses | |
| CN109752541A (en) | A kind of tumor stem cell parting quantitative test card and preparation method thereof and application method | |
| Bedeković et al. | Development of an indirect immunofluorescence assay for diagnosis of bovine viral diarrhoea virus on ear notch tissue samples in cattle infected persistently |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191122 Termination date: 20210125 |