CN209660270U - It is a kind of for saving the cryopreservation device of cell and tissue - Google Patents
It is a kind of for saving the cryopreservation device of cell and tissue Download PDFInfo
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- CN209660270U CN209660270U CN201822238797.5U CN201822238797U CN209660270U CN 209660270 U CN209660270 U CN 209660270U CN 201822238797 U CN201822238797 U CN 201822238797U CN 209660270 U CN209660270 U CN 209660270U
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Abstract
The utility model provides a kind of for saving the cryopreservation device of cell and tissue, comprising: the soaker for freezing bag, freezing frame and being used to exclude to freeze gas in bag after tytosis and tissue；Bag side bottom that freezes is provided with the base opening with turncap, and seal is arranged in top；The frame that freezes includes braced frame and the several layers support plate that horizontal interval is arranged on the support frame, and described freeze after bag is vented is laid on support plate.The cryopreservation device of the utility model has good thermal conductivity, it is easy to use, the characteristics of flexible operation has and realizes fast cooling, rewarming, saves tissue and cell activity, tissue or cell can reach vitrifying with sufficiently fast cooling rate, it can also realize that carrying out rewarming with sufficiently fast speed is very suitable for glass frozen preservation so that glass frozen preservation becomes a reality when recovery, it recovers more convenient, sample easily takes out.
Description
Technical field
The utility model relates to cell and tissue freezing fields, in particular to one kind for saving cell and tissue
Cryopreservation device.
Background technique
Cell cryopreservation is that cell is placed on to low temperature environment, cell metabolism is reduced, so as to a kind of technology of long term storage.Cell
Freezing is one of main method that cell saves, plays the role of cell conservation.
Cell cryopreservation is one of the main method that cell saves, and cell can be made to be temporarily disengaged from growth shape using Cryopreservation Technology
State and its cell characteristics is saved, so when needed again recovery cell for testing.
During cell cryopreservation, especially during glass frozen preservation, mating corresponding cryopreservation device is needed, is adopted at present
Cryopreservation device heating conduction is bad, cannot reach vitrifying with sufficiently fast cooling rate, and when recovery can not be with enough
Fast speed carries out rewarming, is not achieved and preferably freezes effect, is unfavorable for the subsequent applications of cell.Furthermore current cryopreservation device storage
Deposit it is small in size, it is inconvenient when largely freezing, need a large amount of memory spaces, cause storage inconvenient.
In view of this, special propose the utility model.
Utility model content
The purpose of this utility model is to provide a kind of for saving the cryopreservation device of cell and tissue, cryopreservation device tool
There is good thermal conductivity, easy to use, flexible operation has and realizes fast cooling, rewarming, saves the spy of tissue and cell activity
Point, tissue or cell can reach vitrifying with sufficiently fast cooling rate, and when recovery can also be realized with sufficiently fast speed progress
Rewarming is very suitable for glass frozen preservation so that glass frozen preservation becomes a reality, and recovers more convenient, sample easily takes out.
In order to realize the above-mentioned purpose of the utility model, the following technical scheme is adopted:
The utility model provides a kind of for saving the cryopreservation device of cell and tissue, comprising: freeze bag, freeze frame with
And it is used to exclude to freeze the soaker of gas in bag after tytosis and tissue；
Bag side bottom that freezes is provided with the base opening with turncap, and seal is arranged in top；
It is described freeze frame include braced frame and on the support frame horizontal interval arrangement several layers support plate, it is described to freeze
It is laid on support plate after bag exhaust.
Preferably as further enforceable scheme, the seal is made of sack tooth and two sealing strips.
Preferably as further enforceable scheme, the side for freezing bag be provided with frosted information flag window with
And sample-adding mark dotted line.
Preferably as further enforceable scheme, the soaker includes the rubber folder and fastener of closed loop, fastening
Device is made of the folding square plate being connected as one.
Preferably as further enforceable scheme, the rubber folder is constituted by two identical rubber strip folders are folded.
Preferably as further enforceable scheme, the braced frame by bottom level support plate and propping up
On fagging circumferential several support bar groups arranged vertically at.
Preferably as further enforceable scheme, the support plate is rectangular.
Preferably as further enforceable scheme, the support plate is the multilayer of equidistant interval, the support plate with it is described
Support plate is overlapped in the section of vertical direction.
Preferably as further enforceable scheme, it is provided on the support rod and is supporting the support plate socket joint
Accommodation groove on bar.
Preferably as further enforceable scheme, several through-holes have been evenly arranged on the support plate.
Compared with prior art, it is the advantages of the utility model embodiment:
The cryopreservation device provided by the utility model for being used to save cell and tissue, analyzing above-mentioned primary structure can know
Dawn, mainly by freezing bag, freezing frame and three parts of soaker form, this freezes and is provided with seal at the top of bag, bottom
It is provided with base opening, when storing cell, cell solution is poured by bottom, and when storing tissue, tissue is filled by the seal at top
Enter, and be placed on to freeze after being vented by soaker and carry out glass frozen preservation on frame, easy to operate, structure is simple, has and realizes
The characteristics of fast cooling, rewarming, preservation tissue and cell activity, and sample freezes in bag after placement flattening at this, surface area
Greatly, thickness is small, and material thermal conductivity is good, can ensure it is actual freeze effect, the cell after not interfering with rewarming more is subsequent to answer
With being the cryopreservation device for being highly suitable for glass frozen preservation.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
The cryopreservation device that Fig. 1 provides for the utility model embodiment one freezes a bag structural schematic diagram；
Fig. 2 is the soaker structural schematic diagram for the cryopreservation device that the utility model embodiment one provides；
Fig. 3 is the fastener structural schematic diagram in the soaker for the cryopreservation device that the utility model embodiment one provides；
The cryopreservation device that Fig. 4 provides for the utility model embodiment one freezes frame overall structure diagram；
Fig. 5 is the structural schematic diagram for freezing the accommodation groove in frame for the cryopreservation device that the utility model embodiment one provides；
Appended drawing reference:
1- freezes bag；2- soaker；
3- freezes frame；11- base opening；
12- turncap；13- sack tooth；
14- sealing strip；15- information flag window；
16- sample-adding mark dotted line；21- rubber folder；
22- fastener；211- rubber strip；
221- square plate；31- braced frame；
32- support plate；311- support rod；
312- support plate；313- accommodation groove；
321- through-hole.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in the present invention.
The specific structure to cryopreservation device provided by the embodiment of the utility model and particular technique effect are done below
It is described in detail:
The utility model provides a kind of for saving the cryopreservation device of cell and tissue, comprising: freeze bag, freeze frame with
And it is used to exclude to freeze the soaker of gas in bag after tytosis and tissue；
Bag side bottom that freezes is provided with the base opening with turncap, and seal is arranged in top；
It is described freeze frame include braced frame and on the support frame horizontal interval arrangement several layers support plate, it is described to freeze
It is laid on support plate after bag exhaust.
Glass frozen preservation is a kind of mode frozen, has that freeze speed fast, and cell and tissue activity are good etc. after recovery
Feature.However realize that glass frozen preservation needs the fast cooling of cell or tissue.Wherein an important ring for influence cooling rate is
Place the carrier of cell or tissue.
Carrier is frozen used at present as cryopreservation tube or freezes bag, and material is usually PVC material.Material itself it is thermally conductive compared with
Slowly, the thickness of tissue or cell inside it is also typically larger than 1cm, and structure is complicated, and volume is big, and operation is also inconvenient, this makes
Tissue or cell generally can not reach vitrifying with sufficiently fast cooling rate, and when recovery can not be carried out with sufficiently fast speed
Rewarming, so that glass frozen preservation development itself is relatively more limited.
The utility model provides a kind of cryopreservation device, and the cryopreservation device structure is simple, and small in size, flexible operation has
Thermal conductive resin, thickness will not be very thick and heavy inside it for tissue, freezes excellent effect, realizes fast cooling, rewarming to have,
Save tissue and the characteristics of cell activity, be highly suitable for the cryopreservation device of glass frozen preservation, and can take into account cell with
Tissue freezes, and in order to meet the demand for storing different samples, top is provided with seal, bottom is provided with base opening, bottom
There is turncap on mouthful, such structure has fully ensured that certain leakproofness, also ensures and freezes going on smoothly for operating process.
When actual design, freezing bag can be designed as bilayer, and bag outer diameter is respectively as follows: long 19cm [range 18-20cm], wide
16cm [range 15-17cm], internal diameter are long 15cm [range 12-18cm], wide 15cm [range 12-18cm], in the surrounding of bag body
It is additionally provided with sealing edge, sealing edge is 0.5cm [range 0.5-1cm].
Preferably as further enforceable scheme, the seal is made of sack tooth and two sealing strips.
Preferably as further enforceable scheme, the side for freezing bag be provided with frosted information flag window with
And sample-adding mark dotted line, frosted information flag window can be convenient the information of record sample, sample-adding mark dotted line can be convenient instruction
Operator is loaded the position reached.
Preferably as further enforceable scheme, the soaker includes the rubber folder and fastener of closed loop, fastening
Device is made of the folding square plate being connected as one.
Preferably as further enforceable scheme, the rubber folder is constituted by two identical rubber strip folders are folded.
It before it will freeze bag and be placed on and freeze and saved on frame, to be first exhausted, be vented used to bag is frozen
It is the soaker of this specific structure of the utility model.
The operating process being specifically vented are as follows: freeze it is packed enter sample after, sealing strip sealing, base opening is upward, supplement cell
(after the tissue or liquid that are loaded into sack is upright upward, liquid falls into bottom, upper limb to dotted line for solution or semisolid tissue
To dotted line).It freezes bag to be put into soaker, i.e., fills being partially disposed in fastener of liquid or tissue below dotted line, fold
The fastener for the muti-piece square plate composition being connected as one, generally three pieces of square plates can meet requirement, make to freeze adding for bag
At the upper end opening of sample mark dotted line face fastener.Rubber collet is crossed and freezes bag, after being placed in the upper limb of fastener, merges two
Slat gum band is upward, excludes air from bottom to top, finally tightens turncap, will freeze bag and takes out from fastener, is laid flat and freezes bag, will
Cell suspension or group are woven in its interior finger and softly squeeze, pave.It is placed on support plate, insertion freezes in frame, is placed in liquid nitrogen and freezes
It deposits.
Bag will be frozen when recovery and immerses 37 DEG C of water-bath recoveries, and taking out tissue or cell can also take from seal from base opening
Out.
Preferably as further enforceable scheme, the braced frame by bottom level support plate and propping up
On fagging circumferential several support bar groups arranged vertically at.
Preferably as further enforceable scheme, the support plate be it is rectangular, rectangular comparison is firm.
Preferably as further enforceable scheme, the support plate is the multilayer of equidistant interval, the support plate with it is described
Support plate is overlapped in the section of vertical direction, and setting multilayer support plate, which can be stored, more freezes bag.
Preferably as further enforceable scheme, it is provided on the support rod and is supporting the support plate socket joint
Firmly multilayer support plate can be fixed in this way for accommodation groove on bar.
Preferably as further enforceable scheme, several through-holes have been evenly arranged on the support plate.
So-called glass frozen preservation refers to be protected using the glass freezing that the cryoprotector of a variety of high concentrations is jointly formed
Liquid protects suspension cell, and the method that direct plunge into Liquid Nitrogen is frozen does not have ice crystal with the cell suspension that this kind of method is freezed
It is formed, therefore due to being evenly arranged on support plate using liquid nitrogen in order to facilitate liquid nitrogen circulation during glass frozen preservation
There are several through-holes.
The utility model is further described in detail below through specific implementation examples and in conjunction with the accompanying drawings.
Referring specifically to Fig. 1, as specific embodiment, the bottom for freezing 1 side of bag is provided with the base opening with turncap 12
11, seal is arranged in top, and seal is made of sack tooth 13 and two sealing strips 14, if what is saved is that tissue can be from top
Seal be put into, cell suspension is then passed through by the base opening of bottom, is additionally provided with frosted information flag in the side for freezing bag 1
Window 15 and sample-adding mark dotted line 16, to facilitate record information and instruction load position.
Referring to figure 2-3, soaker includes the rubber folder 21 and fastener 22 of closed loop to the specific structure of soaker, wherein rubber
Folder 21 is constituted by two identical folders of rubber strip 211 are folded, and fastener 22 is made of the folding square plate 221 being connected as one,
Preferably 221 numbers of square plate are 3 pieces, after square plate is folded into equilateral triangle by when use, will freeze bag 1 and are put into fastener 22,
The top edge of sample-adding mark 16 face fastener of dotted line, then using rubber press from both sides 21 sets freeze bag 1, merge two slat gum bands
211 upwards, exclude air from bottom to top, finally tighten turncap, will freeze bag 1 and take out from fastener 22, pave and be placed on after exhaust
It freezes on frame 3.
Referring to fig. 4-5, freeze frame 3 includes braced frame 31 and the level in braced frame 31 to the specific structure for freezing frame 3
The several layers support plate 32 being intervally arranged, freeze bag 1 be vented after be laid on support plate 32, braced frame 31 by bottom level support
Plate 312 and on the supporting plate circumferential direction erect straight arranged several support rods 311 composition, and support plate is preferably rectangular, support plate 32
It is just overlapped in the section of vertical direction with support plate 31.
It is provided with the accommodation groove 313 by 32 socket joint of support plate on support rod 311 on support rod 311, such support plate 32 can be with
It is firmly fixed to freeze on frame 1, is subsequently used for placing and freezes bag 1 and frozen.
In addition, being evenly arranged several through-holes 321 on support plate 32, through-hole 321 is evenly distributed, to facilitate glass frozen preservation mistake
The circulation of liquid nitrogen in journey.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, but those skilled in the art answer
Work as understanding: it is still possible to modify the technical solutions described in the foregoing embodiments, either to part of or complete
Portion's technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical
The range of novel each embodiment technical solution.
Claims (10)
1. a kind of for saving the cryopreservation device of cell and tissue characterized by comprising freeze bag, freeze frame and filling out
It fills cell and is used to exclude to freeze the soaker of gas in bag after organizing；
Bag side bottom that freezes is provided with the base opening with turncap, and seal is arranged in top；
The frame that freezes includes braced frame and the several layers support plate that horizontal interval is arranged on the support frame, and the bag that freezes is arranged
It is laid on support plate after gas.
2. cryopreservation device according to claim 1, which is characterized in that the seal is by sack tooth and two sealing strip structures
At.
3. cryopreservation device according to claim 1, which is characterized in that the side for freezing bag is provided with frosted information mark
Remember window and sample-adding mark dotted line.
4. cryopreservation device according to claim 1, which is characterized in that the soaker includes rubber folder and the fastening of closed loop
Device, fastener are made of the folding square plate being connected as one.
5. cryopreservation device according to claim 4, which is characterized in that the rubber folder is pressed from both sides folded by two identical rubber strips
It constitutes.
6. cryopreservation device according to claim 1, which is characterized in that the braced frame by the support plate of bottom level with
And on the supporting plate it is circumferential erect several straight arranged support bar groups at.
7. cryopreservation device according to claim 6, which is characterized in that the support plate is rectangular.
8. cryopreservation device according to claim 6, which is characterized in that the support plate is the multilayer of equidistant interval, the load
Plate is overlapped with the support plate in the section of vertical direction.
9. cryopreservation device according to claim 6, which is characterized in that be provided on the support rod by the support plate socket joint
Accommodation groove on support rod.
10. cryopreservation device according to claim 1, which is characterized in that be evenly arranged several through-holes on the support plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822238797.5U CN209660270U (en) | 2018-12-28 | 2018-12-28 | It is a kind of for saving the cryopreservation device of cell and tissue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822238797.5U CN209660270U (en) | 2018-12-28 | 2018-12-28 | It is a kind of for saving the cryopreservation device of cell and tissue |
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| CN209660270U true CN209660270U (en) | 2019-11-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
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2018
- 2018-12-28 CN CN201822238797.5U patent/CN209660270U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
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