CN209652339U - A kind of CAR-T Tissue Culture Dish - Google Patents
A kind of CAR-T Tissue Culture Dish Download PDFInfo
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- CN209652339U CN209652339U CN201822277773.0U CN201822277773U CN209652339U CN 209652339 U CN209652339 U CN 209652339U CN 201822277773 U CN201822277773 U CN 201822277773U CN 209652339 U CN209652339 U CN 209652339U
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Abstract
The utility model discloses a kind of CAR-T Tissue Culture Dish, including Tissue Culture Dish ontology, Tissue Culture Dish ontology includes interior ware, outer ware and ware lid, it is connected between interior ware and outer ware by post-filter drainage device, including the first leakage fluid dram and the second leakage fluid dram, the upper end of first leakage fluid dram is provided with filter screen, agitating device is provided on outer ware, agitating device includes pedestal, cylinder and spring, the lower section of outer ware is arranged in pedestal, the output end of cylinder is connected with elevating lever, the bottom surface of the other end of elevating lever and outer ware is articulated and connected, spring is uniformly distributed along the circumferencial direction of pedestal, electromagnet is provided on the side wall of outer ware, one, which is constituted, by the cooperation of the first leakage fluid dram and the second leakage fluid dram dumps culture medium waste fluid channel, culture medium replacement is quick, it is convenient, cylinder pushes elevating lever to rise or fall, rock Tissue Culture Dish ontology, Spring provides buffering, slows down and rocks intensity, T cell is made to come into full contact with magnetic bead to adhere to combination.
Description
Technical field
The utility model relates to field of cell culture, in particular to a kind of CAR-T Tissue Culture Dish.
Background technique
CAR-T, full name are Chimeric Antigen Receptor T-Cell Immunotherapy, chimeric antigen by
Body T cell immunotherapy, this is one and many years occurs, but is just modified use novel cell clinically in recent years
Therapy has significant curative effect in the treatment of acute leukemia and non-Hodgkin lymphoma, it is considered to be most promising swollen
One of tumor therapeutic modality, technology as in all is the same, and CAR-T technology also undergoes a very long evolutionary process, exactly at this
In a series of evolutionary process, CAR-T technology gradually moves to maturity, and needs to use CAR-T cell in CAR-T cell culture
Culture dish.
Tissue Culture Dish needs the replacement of cleaning position progress culture medium during cultivating CAR-T cell, rather than closed
Environment be easy to cause pollution, cause the replacement of culture medium inconvenient, there is the magnetic bead of CD3 or CD28 antibody in when CAR-T cell culture
Specific adsorption T lymphocyte needs manually to shake container to realize sufficiently connecing for T cell and magnetic bead in this step of magnetic bead surfaces
Touching, but manual operation is time-consuming and laborious, and be easy to cause adsorption rate low, influences the culture effect of CAR-T cell
Summary of the invention
For the deficiencies in the prior art, the purpose of this utility model is to provide a kind of replacement culture mediums conveniently,
Time saving and energy saving and T cell and the higher CAR-T Tissue Culture Dish of the adsorption rate of magnetic bead.
The technical issues of the utility model solves can be realized using following technical scheme:
A kind of CAR-T Tissue Culture Dish, including Tissue Culture Dish ontology；The Tissue Culture Dish ontology includes interior ware, outer
Ware and ware lid, the rounded structure in the cross section of interior ware, outer ware and ware lid, interior ware are socketed in the inside of outer ware, interior ware and ware lid
Between form a closed accommodating cavity, and the upper end of interior ware extends outwardly and is provided with baffle, and the bottom of ware lid is provided with card
The opening inside diameter of plate, snap-gauge and interior ware matches, and the outer diameter of interior ware and the internal diameter of outer ware match.
It is connected between the interior ware and outer ware by post-filter drainage device.
The post-filter drainage device includes the first leakage fluid dram for being provided with interior ware bottom, and the first leakage fluid dram is quadrant
Sector structure, and the center line of ware is center of circle setting within the first leakage fluid dram, and the upper end of the first leakage fluid dram is provided with filter screen,
Post-filter drainage device further includes the second leakage fluid dram that outer ware bottom is arranged in, and the center of circle of the second leakage fluid dram and the first leakage fluid dram
The center of circle is overlapped, and the lower end of the second leakage fluid dram is communicated with Drainage pipe, and Drainage pipe extends the outer wall of outer ware, passes through the first drain
Mouth and second row
The cooperation of liquid mouth may be constructed the switch for dumping culture medium waste fluid channel, i.e., when the first leakage fluid dram and second
When leakage fluid dram is connected to, removal waste fluid channel is opened, and when the first leakage fluid dram and the second leakage fluid dram misplace, removal waste fluid channel is closed, culture
Base replacement quickly, conveniently, improves the reproductive efficiency of CAR-T cell.
Ware in rotating first, is subjected to displacement interior ware relative to outer ware, until when the first leakage fluid dram and the second leakage fluid dram are connected to,
Removal waste fluid channel is opened, and culture medium waste liquid is flowed out from interior ware, successively passes through the first leakage fluid dram, the second leakage fluid dram and Drainage pipe
After be discharged, continue to rotate interior ware, until the first leakage fluid dram and the second leakage fluid dram misplace, removal waste fluid channel is closed, and completes drain, then
The inlet in ware lid is opened, new culture medium is passed through by inlet.
Agitating device is provided on the outer ware, agitating device includes pedestal, cylinder and spring, and outer ware is arranged in pedestal
Lower section, and the center of circle direction for deviateing outer ware in pedestal inlays and is provided with cylinder, the output end of cylinder is connected with elevating lever, elevating lever
The other end and the bottom surface of outer ware be articulated and connected, one end of spring is fixedly connected with outer ware, and the other end is fixedly connected with pedestal, and
It is uniformly distributed along the circumferencial direction of pedestal, electromagnet is provided on the side wall of outer ware, cylinder is accessed into external power supply first, so
Afterwards open cylinder switch, cylinder push elevating lever rise or fall, telescopic rod push upper end Tissue Culture Dish ontology along
Hinged direction is rocked, and the buffering for being set as cylinder and pushing offer certain of peripheral spring slows down what Tissue Culture Dish ontology rocked
Intensity enables T cell to come into full contact with magnetic bead and obtains longer attachment binding time, improves the culture efficiency of CAR-T cell.
Above-mentioned CAR-T Tissue Culture Dish is fixed between the interior ware and snap-gauge by helicitic texture, and helicitic texture includes
The internal screw thread being arranged on the inner wall of the opening of interior ware and the external screw thread being arranged on the outer wall of snap-gauge lead between interior ware and ware lid
It crosses helicitic texture to be fixedly connected, realizes Singapore being bonded between the two, it is empty to improve receiving internal between interior ware and ware lid
Between leakproofness.
Above-mentioned CAR-T Tissue Culture Dish, is provided with position limiting structure between the interior ware and outer ware, position limiting structure includes setting
It sets symmetrical in the movable block on the bottom surface that baffle stretches out outer ware one end and the limited block on the outer wall that outer ware is arranged in, limited block
The outer wall both ends of outer ware are set, by the cooperation in position limiting structure between movable block and limited block, may be implemented primary complete
The first leakage fluid dram and the second leakage fluid dram from each other be connected to the shifting process to misplace completely completely.
Above-mentioned CAR-T Tissue Culture Dish, one end that the Drainage pipe stretches out the outer wall of outer ware are connect with tapping valve.
The radius of above-mentioned CAR-T Tissue Culture Dish, second leakage fluid dram is equal to or less than the first leakage fluid dram.
The beneficial effects of the utility model are: passing through the cooperation of the first leakage fluid dram and the second leakage fluid dram, one may be constructed
Dump the switch of culture medium waste fluid channel, i.e., when the first leakage fluid dram is connected to the second leakage fluid dram, removal waste fluid channel is opened, and first
When leakage fluid dram and the second leakage fluid dram misplace, removal waste fluid channel is closed, and culture medium replacement quickly, conveniently, improves CAR-T cell
Reproductive efficiency, cylinder push elevating lever to rise or fall, and telescopic rod pushes the Tissue Culture Dish ontology of upper end along hinged direction
It rocks, the buffering for being set as cylinder and pushing offer certain of peripheral spring slows down the intensity that Tissue Culture Dish ontology rocks, so that
T cell can come into full contact with magnetic bead and obtain longer attachment binding time, improve the culture efficiency of CAR-T cell.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
By make the structure feature to the utility model and the effect of being reached have it is further understand and understanding, to compared with
Good examples and drawings cooperate detailed description, are described as follows:
As shown in Figure 1, a kind of CAR-T Tissue Culture Dish, including Tissue Culture Dish ontology.
The Tissue Culture Dish ontology includes interior ware 1, outer ware 2 and ware lid 3, and the cross section of interior ware, outer ware and ware lid is in
Circular configuration, interior ware are socketed in the inside of outer ware, a closed accommodating cavity are formed between interior ware and ware lid, and interior ware is upper
End, which extends outwardly, is provided with baffle 11, and the bottom of ware lid is provided with snap-gauge 31, and the opening inside diameter of snap-gauge and interior ware matches, interior ware
The internal diameter of outer diameter and outer ware match.
It is connected between the interior ware and outer ware by post-filter drainage device.
The post-filter drainage device includes the first leakage fluid dram 41 for being provided with interior ware bottom, and the first leakage fluid dram is a quarter
Round sector structure, and the center line of ware is center of circle setting within the first leakage fluid dram, the upper end of the first leakage fluid dram is provided with filtering
Net, post-filter drainage device further include the second leakage fluid dram 42 that outer ware bottom is arranged in, and the center of circle of the second leakage fluid dram and first row
The center of circle of liquid mouth is overlapped, and the lower end of the second leakage fluid dram is communicated with Drainage pipe 43, and Drainage pipe extends the outer wall of outer ware, passes through
The cooperation of first leakage fluid dram and the second leakage fluid dram may be constructed the switch for dumping culture medium waste fluid channel, that is, work as first row
When liquid mouth is connected to the second leakage fluid dram, removal waste fluid channel is opened, when the first leakage fluid dram and the second leakage fluid dram misplace, removal waste fluid channel
It closes, culture medium replacement quickly, conveniently, improves the reproductive efficiency of CAR-T cell.
Ware in rotating first, is subjected to displacement interior ware relative to outer ware, until when the first leakage fluid dram and the second leakage fluid dram are connected to,
Removal waste fluid channel is opened, and culture medium waste liquid is flowed out from interior ware, successively passes through the first leakage fluid dram, the second leakage fluid dram and Drainage pipe
After be discharged, continue to rotate interior ware, until the first leakage fluid dram and the second leakage fluid dram misplace, removal waste fluid channel is closed, and completes drain, then
The inlet in ware lid is opened, new culture medium is passed through by inlet.
Agitating device is provided on the outer ware, agitating device includes pedestal 51, cylinder 52 and spring 53, and pedestal setting exists
The lower section of outer ware, and the center of circle direction for deviateing outer ware in pedestal inlays and is provided with cylinder, the output end of cylinder is connected with elevating lever
54, the bottom surface articulated connection of the other end of elevating lever and outer ware, one end of spring is fixedly connected with outer ware, and the other end and pedestal are solid
Fixed connection, and be uniformly distributed along the circumferencial direction of pedestal, electromagnet 55 is provided on the side wall of outer ware, first by cylinder and electricity
Magnet accesses external power supply, then opens the switch of cylinder, and cylinder pushes elevating lever to rise or fall, and telescopic rod pushes upper end
Tissue Culture Dish ontology is rocked along hinged direction, and the buffering for being set as cylinder and pushing offer certain of peripheral spring slows down thin
The intensity that born of the same parents' culture dish ontology rocks enables T cell to come into full contact with magnetic bead and obtains longer attachment binding time, improves
The culture efficiency of CAR-T cell.
In the present invention, it is fixed between the interior ware and snap-gauge by helicitic texture, helicitic texture includes that setting exists
Internal screw thread on the inner wall of the opening of interior ware and the external screw thread being arranged on the outer wall of snap-gauge, pass through screw thread between interior ware and ware lid
Structure is fixedly connected, and realizes Singapore being bonded between the two, improves the close of accommodation space internal between interior ware and ware lid
Feng Xing.
Position limiting structure is provided between the interior ware and outer ware, position limiting structure includes that baffle is arranged in stretch out outer ware one end
Movable block on bottom surface and the limited block 32 being arranged on the outer wall of outer ware, limited block are symmetrical arranged the outer wall two in outer ware
Primary complete first leakage fluid dram and second may be implemented by the cooperation in position limiting structure between movable block and limited block in end
Leakage fluid dram from each other be connected to the shifting process to misplace completely completely.
The Drainage pipe stretches out one end of the outer wall of outer ware as a further improvement of the utility model, and tapping valve connects
It connects.
The radius of second leakage fluid dram is equal to or less than the first leakage fluid dram.
By the cooperation of the first leakage fluid dram and the second leakage fluid dram, it may be constructed one and dump opening for culture medium waste fluid channel
It closes, i.e., when the first leakage fluid dram is connected to the second leakage fluid dram, removal waste fluid channel is opened, the first leakage fluid dram and the dislocation of the second leakage fluid dram
When, removal waste fluid channel is closed, and the reproductive efficiency of CAR-T cell is improved, and cylinder pushes elevating lever to rise or fall, and telescopic rod pushes away
The Tissue Culture Dish ontology of dynamic upper end is rocked along hinged direction, and the cylinder that is set as of peripheral spring pushes offer is certain to delay
Punching, slows down the intensity that Tissue Culture Dish ontology rocks, when enabling T cell to come into full contact with magnetic bead and obtaining longer attachment combination
Between, improve the culture efficiency of CAR-T cell.
Basic principles, main features, and advantages of the present invention has been shown and described above.Current row
The technical staff of industry is described in above embodiments and description it should be appreciated that the present utility model is not limited to the above embodiments
Only the principles of the present invention, the utility model also has respectively on the premise of not departing from the spirit and scope of the utility model
Kind changes and improvements, these changes and improvements are both fallen in the range of claimed invention.The requires of the utility model
Protection scope defined by appended claims and its equivalent.
Claims (5)
1. a kind of CAR-T Tissue Culture Dish, including Tissue Culture Dish ontology；
It is characterized in that,
The Tissue Culture Dish ontology includes interior ware, outer ware and ware lid, the rounded knot in the cross section of interior ware, outer ware and ware lid
Structure, interior ware are socketed in the inside of outer ware, a closed accommodating cavity are formed between interior ware and ware lid, and the upper end of interior ware is outside
Be extended have baffle, the bottom of ware lid is provided with snap-gauge, and the opening inside diameter of snap-gauge and interior ware matches, the outer diameter of interior ware with outside
The internal diameter of ware matches；
It is connected between the interior ware and outer ware by post-filter drainage device；
The post-filter drainage device includes the first leakage fluid dram for being provided with interior ware bottom, and the first leakage fluid dram is the fan of quadrant
Shape structure, and the center line of ware is center of circle setting within the first leakage fluid dram, the upper end of the first leakage fluid dram is provided with filter screen, filters
Pumping equipment further includes the second leakage fluid dram that outer ware bottom is arranged in, and the center of circle in the center of circle of the second leakage fluid dram and the first leakage fluid dram
It is overlapped, the lower end of the second leakage fluid dram is communicated with Drainage pipe, and Drainage pipe extends the outer wall of outer ware；
Agitating device is provided on the outer ware, agitating device includes pedestal, cylinder and spring, and pedestal is arranged under outer ware
Side, and the center of circle direction for deviateing outer ware in pedestal inlays and is provided with cylinder, the output end of cylinder is connected with elevating lever, elevating lever
The bottom surface of the other end and outer ware is articulated and connected, and one end of spring is fixedly connected with outer ware, and the other end is fixedly connected with pedestal, and edge
The circumferencial direction of pedestal be uniformly distributed, be provided with electromagnet on the side wall of outer ware.
2. CAR-T Tissue Culture Dish according to claim 1, which is characterized in that pass through spiral shell between the interior ware and snap-gauge
Line structure is fixed, and helicitic texture includes internal screw thread on the inner wall for the opening that interior ware is arranged in and is arranged on the outer wall of snap-gauge
External screw thread.
3. CAR-T Tissue Culture Dish according to claim 1, which is characterized in that be provided between the interior ware and outer ware
Position limiting structure, position limiting structure include that the movable block and the outer of outer ware is arranged in that baffle stretches out on the bottom surface of outer ware one end is arranged in
Limited block on wall, limited block are symmetrical arranged at the outer wall both ends of outer ware.
4. CAR-T Tissue Culture Dish according to claim 1, which is characterized in that the Drainage pipe stretches out the outer of outer ware
One end of wall is connect with tapping valve.
5. CAR-T Tissue Culture Dish according to claim 1, which is characterized in that the radius of second leakage fluid dram is equal to
Or less than the first leakage fluid dram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822277773.0U CN209652339U (en) | 2018-12-29 | 2018-12-29 | A kind of CAR-T Tissue Culture Dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822277773.0U CN209652339U (en) | 2018-12-29 | 2018-12-29 | A kind of CAR-T Tissue Culture Dish |
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| Publication Number | Publication Date |
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| CN209652339U true CN209652339U (en) | 2019-11-19 |
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| CN201822277773.0U Active CN209652339U (en) | 2018-12-29 | 2018-12-29 | A kind of CAR-T Tissue Culture Dish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
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2018
- 2018-12-29 CN CN201822277773.0U patent/CN209652339U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
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