CN209584274U - A kind of clinical Endometrial stem cell is separately cultured device - Google Patents

Abstract

Device is separately cultured the utility model discloses clinical Endometrial stem cell a kind of in stem cell culturing apparatus technical field, including bottom chamber, motor and rotation axis etc., the culture solution of Endometrial stem cell is configured in culture groove body, after configuration, close big closing cap, it can make the continuous intermittent rotary of driven turntable by motor work, cultivate groove body intermittent rotary therewith on driven turntable, intermittent rotary can increase contact of the culture cell with culture solution, improve nutrition supply, under conditions of rotation, it can avoid the harmful substance that cell metabolism generates and murder by poisoning generated to cell itself in local accumulation, pass through carrying handle, piston rod is driven to move up, piston moves up therewith, the culture solution for the Endometrial stem cell cultivated in groove body is drawn in shell, separator is removed from opening portion.

Description

A kind of clinical Endometrial stem cell is separately cultured device

Technical field

The utility model relates to stem cell culturing apparatus technical field, specific field is a kind of clinical dry thin with endometrium Born of the same parents' is separately cultured device.

Background technique

Endometrial stem cell is palace hemocytoblast, and palace hemocytoblast is that self-replacation, directed differentiation ability are strongest Stem cell, it is derived from endometrium, is excreted by menses, its differentiation potential is close with embryonic stem cell, but also with exempting from Epidemic focus is low, and the heteroplastic transplantation characteristic weaker without immunological rejection or reaction can be used for treating a variety of diseases, palace hemocytoblast Acquisition is simple, is not required to distribution type, uses for the people of no blood source relationship, at present it can be confirmed that the medicable disease of palace hemocytoblast has Injury of lungs, cirrhosis, heart infarction and diabetes etc., pertinent art technically constantly explore, for convenient for dry to endometrium The correlative study of cell, propose a kind of clinical Endometrial stem cell is separately cultured device.

Utility model content

It is separately cultured device the purpose of this utility model is to provide a kind of clinical Endometrial stem cell, to solve The problems mentioned above in the background art.

To achieve the above object, the utility model provides the following technical solutions: a kind of clinical Endometrial stem cell It is separately cultured device, including bottom chamber, the inside of the bottom chamber is equipped with motor, and the output end axis connection of the motor has Rotation axis, the upper end of the rotation axis are fixedly connected with lower rotating disc, and the upper surface of the lower rotating disc is fixedly connected with rotation Turntable, the side of the upper rotating disk are equipped with upper fixed column, the fixed company in the upper surface of fixed column and the lower rotating disc Connect, the upper wall left end of the bottom chamber is equipped with fixing axle, and the upper end of the fixing axle is rotatably connected to driven turntable, it is described from The lateral wall of turn disk is equipped with strip slot uniformly integrally formed with flabellum between two adjacent flabellums, described from turn The upper surface of disk is fixedly connected with clamping groove, and culture groove body is connected in the clamping groove, is divided on the side wall of the culture groove body Not She You nitrogen inlet duct, nitrogen out of the trachea and permeability cell, the nitrogen inlet duct, the nitrogen out of the trachea and the permeability cell On be threaded with small closing cap, the upper end of the culture groove body is connected with big closing cap, and the big closing cap is equipped with and opens Oral area, through separator is equipped in the opening portion, the separator includes shell, and the inside of the shell, which is equipped with, lives Plug, the upper end of the piston are fixedly connected with piston rod, and the upper end of the piston rod is fixedly connected with handle, the shell it is upper End lateral wall is fixedly connected with limit handle, and the lower end of the shell, which is equipped with, draws tubule, and the absorption tubule is located at the culture The inner cavity lower part of groove body.

Preferably, the upper rotating disk is in 3/4ths circle handle.

Preferably, the lateral wall of the flabellum is in concave arc shape.

Preferably, the big closing cap is equipped with rubber seal.

Compared with prior art, the utility model has the beneficial effects that a kind of separation of clinical Endometrial stem cell Culture apparatus configures the culture solution of Endometrial stem cell in culture groove body, after configuration, closes big closing cap, pass through Motor work can drive rotation axis to rotate, and lower rotating disc and upper rotating disk rotate with it, and the side wall of upper rotating disk is located at from turn The side of disk rotates, and when its a quarter indentation, there rotates to the side of one of flabellum, fixed column enters strip slot In, with the rotation of upper rotating disk, fixed column can stir driven turntable and rotate in fixing axle upper end, as a result, can by motor work Make the continuous intermittent rotary of driven turntable, cultivating groove body, intermittent rotary, intermittent rotary can increase culture carefully therewith on driven turntable Contact of the born of the same parents with culture solution improves nutrition supply, under conditions of rotation, can avoid the harmful substance of cell metabolism generation in office Portion accumulates and generates murder by poisoning to cell itself, when needing the culture solution separation to Endometrial stem cell, needs to open ventilative Small closing cap on pipe drives piston rod to move up by carrying handle, and piston moves up therewith, will cultivate the intrauterine in groove body The culture solution of film stem cell is drawn in shell, and separator is removed from opening portion.

Detailed description of the invention

Fig. 1 is the main structure main view of the utility model；

Fig. 2 is the lower rotating disc, upper rotating disk, upper fixed column, fixing axle, driven turntable, flabellum and item of the utility model Shape slot top view；

Fig. 3 is the big closure cap structure schematic diagram of the utility model.

In figure: 1- bottom chamber, 2- motor, 3- rotation axis, 4- lower rotating disc, the upper rotating disk of 5-, the upper fixed column of 6-, 7- are solid Dead axle, 8- driven turntable, 9- flabellum, 10- strip slot, 11- clamping groove, 12- cultivate groove body, 13- nitrogen inlet duct, 14- nitrogen Escape pipe, 15- permeability cell, the small closing cap of 16-, the big closing cap of 17-, the opening portion 18-, 19- separator, 191- shell, 192- Piston, 193- piston rod, 194- handle, 195- limit handle, 196- draw tubule.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

Fig. 1-3 is please referred to, the utility model provides a kind of technical solution: a kind of separation of clinical Endometrial stem cell The inside of culture apparatus, including bottom chamber 1, the bottom chamber is equipped with motor 2, and the bottom chamber can be used as clinical use The pedestal for being separately cultured device of Endometrial stem cell, while can be used for placing the motor, the output end of the motor again Axis connection has rotation axis 3, and the upper end of the rotation axis is fixedly connected with lower rotating disc 4, and the upper surface of the lower rotating disc is fixed It is connected with rotating disk 5, the rotation axis can be driven to rotate by motor work, the lower rotating disc and the upper rotation Disk rotates with it, and the side of the upper rotating disk is equipped with upper fixed column 6, the upper surface of fixed column and the lower rotating disc It is fixedly connected, the upper wall left end of the bottom chamber is equipped with fixing axle 7, and the upper end of the fixing axle is rotatably connected to driven turntable 8, the lateral wall of the driven turntable is equipped with strip slot 10 uniformly integrally formed with flabellum 9 between two adjacent flabellums, The side wall of the upper rotating disk is located at the side rotation of the driven turntable, when its a quarter indentation, there rotates to one of them When the side of the flabellum, the fixed column is entered in the strip slot, with the rotation of the upper rotating disk, the fixed column The driven turntable can be stirred to rotate in the fixing axle upper end, the driven turntable can be made by motor work as a result, Continuous intermittent rotary, the upper surface of the driven turntable are fixedly connected with clamping groove 11, are connected with culture tank in the clamping groove Body 12, the clamping groove are used to for the culture groove body being fixed on the driven turntable, divide on the side wall of the culture groove body Not She You nitrogen inlet duct 13, nitrogen out of the trachea 14 and permeability cell 15, the nitrogen inlet duct, the nitrogen out of the trachea and described Small closing cap 16 is threaded on permeability cell, when needing oxygen-free environment in the culture groove body, the openable nitrogen Nitrogen source output terminal is connected to the nitrogen inlet duct end, thus by the small closing cap in air inlet pipe and the nitrogen out of the trachea The intracorporal air of the culture tank can be discharged, make in the culture tank body in anaerobic state, the upper end clamping of the culture groove body There is big closing cap 17, for the big closing cap for sealing the culture groove body, the big closing cap is equipped with opening portion 18, described Through separator 19 is equipped in opening portion, the separator is used to draw and shift the culture solution of Endometrial stem cell, The separator includes shell 191, and the inside of the shell is equipped with piston 192, and the upper end of the piston is fixedly connected with work Stopper rod 193, the upper end of the piston rod are fixedly connected with handle 194, and the upper end lateral wall of the shell is fixedly connected with limit Handle 195, for limiting the separator in the position of the opening portion, the lower end of the shell is equipped with draws the limit handle Tubule 196, the tubule of drawing are located at the inner cavity lower part for cultivating groove body, by the lifting handle, drive the piston Bar moves up, and the piston moves up therewith, the culture solution of the Endometrial stem cell in the culture groove body is drawn to described outer In shell, separator is removed from the opening portion.

Specifically, the upper rotating disk is worked by the motor in 3/4ths circle handle, drives the rotation axis Rotation, the lower rotating disc and the upper rotating disk rotate with it, and the side wall of the upper rotating disk is located at the driven turntable Side rotation, when its a quarter indentation, there rotates to the side of one of them flabellum, the fixed column enters institute It states in strip slot, with the rotation of the upper rotating disk, the fixed column can stir the driven turntable in the fixing axle upper end Rotation can make the continuous intermittent rotary of the driven turntable by motor work as a result,.

Specifically, the lateral wall of the flabellum is in concave arc shape, worked by the motor, the rotation axis is driven to revolve Turn, the lower rotating disc and the upper rotating disk rotate with it, and the side wall of the upper rotating disk is located at the one of the driven turntable Side rotation, when at the side concave arc that its a quarter indentation, there rotates to one of them flabellum, the fixed column enters Into the strip slot, with the rotation of the upper rotating disk, the fixed column can stir the driven turntable in the fixing axle Upper end rotation can make the continuous intermittent rotary of the driven turntable by motor work as a result,.

Specifically, the big closing cap is equipped with rubber seal, the sealing ring makes the big closing cap to described Cultivating groove body has preferable sealing function.

Working principle: configuring the culture solution of Endometrial stem cell in culture groove body first, and after configuration, closing is big Closing cap can drive rotation axis to rotate by motor work, and lower rotating disc and upper rotating disk rotate with it, the side wall of upper rotating disk Positioned at driven turntable side rotate, when its a quarter indentation, there rotates to the side of one of flabellum, fixed column into Enter into strip slot, with the rotation of upper rotating disk, fixed column can stir driven turntable and rotate in fixing axle upper end, pass through as a result, Motor work can make the continuous intermittent rotary of driven turntable, and intermittent rotary, intermittent rotary can therewith on driven turntable for culture groove body Increase contact of the culture cell with culture solution, improve nutrition supply, under conditions of rotation, can avoid having for cell metabolism generation Evil substance generates murder by poisoning to cell itself in local accumulation, when needing the culture solution separation to Endometrial stem cell, needs The small closing cap on permeability cell is opened, then by carrying handle, drives piston rod to move up, piston moves up therewith, will cultivate The culture solution of Endometrial stem cell in groove body is drawn in shell, and separator is removed from opening portion.

While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (4)

1. a kind of clinical Endometrial stem cell is separately cultured device, including bottom chamber (1), it is characterised in that: described The inside of bottom chamber (1) is equipped with motor (2), and the output end axis connection of the motor (2) has rotation axis (3), the rotation axis (3) upper end is fixedly connected with lower rotating disc (4), and the upper surface of the lower rotating disc (4) is fixedly connected with rotating disk (5), The side of the upper rotating disk (5) is equipped with upper fixed column (6), the upper surface of fixed column (6) and the lower rotating disc (4) It is fixedly connected, the upper wall left end of the bottom chamber (1) is equipped with fixing axle (7), and the upper end of the fixing axle (7) is rotatably connected to Driven turntable (8), the lateral wall of the driven turntable (8) is uniformly integrally formed with flabellum (9), two adjacent flabellums (9) strip slot (10) are equipped between, the upper surface of the driven turntable (8) is fixedly connected with clamping groove (11), the clamping groove (11) it is connected with culture groove body (12) in, is respectively equipped with nitrogen inlet duct (13), nitrogen on the side wall of culture groove body (12) Escape pipe (14) and permeability cell (15), the nitrogen inlet duct (13), the nitrogen out of the trachea (14) and the permeability cell (15) On be threaded with small closing cap (16), it is described culture groove body (12) upper end be connected with big closing cap (17), the big envelope Lid (17) are closed equipped with opening portion (18), the opening portion (18) is interior through equipped with separator (19), the separator It (19) include shell (191), the inside of the shell (191) is equipped with piston (192), and the upper end of the piston (192) is fixed to be connected It is connected to piston rod (193), the upper end of the piston rod (193) is fixedly connected with handle (194), the upper end of the shell (191) Lateral wall is fixedly connected with limit handle (195), and the lower end of the shell (191), which is equipped with, draws tubule (196), the absorption tubule (196) it is located at the inner cavity lower part of culture groove body (12).

2. a kind of clinical Endometrial stem cell according to claim 1 is separately cultured device, it is characterised in that: institute Rotating disk (5) are stated in 3/4ths circle handle.

3. a kind of clinical Endometrial stem cell according to claim 1 is separately cultured device, it is characterised in that: institute The lateral wall of flabellum (9) is stated in concave arc shape.

4. a kind of clinical Endometrial stem cell according to claim 1 is separately cultured device, it is characterised in that: institute Big closing cap (17) are stated equipped with rubber seal.