CN209564438U - A kind of magnesium wire silk composite braided structures nerve trachea - Google Patents
A kind of magnesium wire silk composite braided structures nerve trachea Download PDFInfo
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- CN209564438U CN209564438U CN201822252819.3U CN201822252819U CN209564438U CN 209564438 U CN209564438 U CN 209564438U CN 201822252819 U CN201822252819 U CN 201822252819U CN 209564438 U CN209564438 U CN 209564438U
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- nerve trachea
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- Media Introduction/Drainage Providing Device (AREA)
- Materials For Medical Uses (AREA)
Abstract
The utility model discloses a kind of magnesium wire silk composite braided structures nerve tracheas, the medical metal magnesium wire with stronger mechanical property is used to be added in silk braiding structure for axis yarn, both play the role of longitudinal guide, the radial crush resistance energy of braiding skeleton can be improved again, simultaneously, magnesium wire can slowly degrade, and promote the growth and proliferation of schwann cell, be conducive to the recovery of injuring nerve；Braiding yarn is boiled silk, has good biocompatibility, while guaranteeing good mechanical properties, forms the biological microenvironment for promoting nerve regneration.
Description
Technical field
The utility model belongs to biomedical material technology, and in particular to a kind of magnesium wire silk composite braided structures mind
Through conduit.
Background technique
Peripheral nerve injury is clinically common illness, promotes defect nerve using biomaterial and tissue engineering technique
It repairs and regeneration is the research hotspot of neuroscience field.Ideal biomaterial, suitable physical mechanics guide and are suitable for
Biological microenvironment is that injured nerve is promoted to restore the key factor of biological functions such as to feel, move.Nerve trachea is studied at present
More be using model casting method, electrostatic nanometer spin processes, 3D biometric print molding etc. technologies, and using fibroin albumen,
The biodegradable materials such as chitosan and collagen produce the hollow conduit with physical guide effect, but these technologies
The Mechanics of Machinery and flexibility of the nerve trachea prepared are poor.Such as a kind of nanofiber directional guide function using fibroin
Nerve trachea uses condensation induction silk fibroin solution to form nanometer micropore as physical guide, but formed in the utility model
Micropore is difficult to control shape size and direction, and the radial crush resistance of conduit can be general, and bending suppleness is not considered；For another example
The nerve trachea of tubular structure, such nerve trachea are first then rolled into the film that different degradation concentration are made in nanometer orientation fiber
It is excellent to the physics guiding performance of cell, but mechanical performance itself is poor, and the internal space for nerve regneration is less；For another example 3D is used
Biometric print has biggish limitation to material, and resolution ratio is lower at present, is probably 1 ~ 300 mPa/s.
The study found that magnesium elements are one of important elements needed by human, egg is participated in brain and central nervous system
The synthesis of white matter activates a variety of enzymes in vivo, adjusts nervimuscular activity.Under microenvironment, metal magnesium ion has anti-inflammatory, anti-
The multiple biological functions such as oxidation, anti-apoptotic and adjusting mitochondrial calcium buffering.It recent studies have shown that magnesium ion is micro- be suitable for
It can promote the proliferation of schwann cell under the culture of environment, such as secrete growth factor, extracellular matrix (Extracellular
Matrix, ECM) etc., play the role of support and protection to neuron.Domestic silkworm silk is the natural animal synthesized by silkworm with secretion
Albumen, from a wealth of sources, fibroin albumen is main component part in silk, accounts for about the 70%-80% of silk quality, remaining mainly at
It is divided into sericin and carbohydrate etc..Fibroin albumen contains there are many amino acid, wherein relatively common alanine (Ala),
Glycine (Gly), serine (Ser) account for about the 85% of total composition.Fibroin itself has good physicochemical property, air-moisture-permeable, life
Object compatibility, energy degradation in vivo are that polypeptide and free amino acid can be absorbed by organisms to promote the growth of nerve cell.
Inflammatory reaction is low in nerve trachea body made of fibroin albumen, biodegradable, can promote the functionality of peripheral nerve injury
Restore.Chitosan is also known as chitosan, is that the chitin (chitin) being widely present by nature passes through deacetylation
It obtains, structural property and glycosaminoglycan in ECM are very much like, and adsorptivity is good, and degradation speed is adjustable, in degradation
Between product chitosan oligosaccharide can inhibit Neuron Apoptosis, sertoli cell is sticked, and injured axon regeneration is promoted.
The common technique for being used to prepare nerve trachea has: (1) casting-infusion process, this method is although easy to operate, but props up
Toxic solvent, and the size and distribution of hole not easy to control are easily remained on frame；(2) conventional electrostatic spining technology can form high hole
The nanofiber of gap rate and specific surface area can produce the microenvironment of similar cell epimatrix reticular structure, be conducive to nerve again
Growth, but the nerve trachea mechanical strength that conventional electrostatic spinning is produced is insufficient, and stable mechanics can not be provided for injured nerve
Environment；(3) knitting skill, the technology use spindle braider, so that yarn is woven into tubing, it is possible to increase the table of catheter interior
Area, mechanical property are preferable.But in terms of inducing cell directional migration and regeneration, still there is shortcoming.
In response to the above problems, the present invention proposes a kind of magnesium wire silk composite braided structures nerve tracheas.
Utility model content
The utility model aim is: providing a kind of magnesium wire silk composite braided structures nerve trachea, can improve nerve regneration
Order and nerve trachea mechanical strength, to solve the above problems.
A kind of technical solution of the utility model is:
A kind of magnesium wire silk composite braided structures nerve trachea, including nerve trachea outer layer, nerve trachea middle layer and mind
Through conduit internal layer, the nerve trachea middle layer is coated on the outside of the nerve trachea internal layer, the nerve trachea outer layer packet
It is overlying on the outside of the nerve trachea middle layer, the nerve trachea outer layer is mandruka structure, among the nerve trachea
Layer is three-dimensional braided structure, and the nerve trachea internal layer is porous gel structure.
Further, the nerve trachea outer layer with a thickness of 0.5-1.5mm, aperture is 20-150 μm, and porosity is
75%-90%。
Further, the three-dimensional braided structure is diamond weave structure, regular braiding structure and He Gelisi braiding knot
Any one in structure.
Further, the nerve trachea middle layer with a thickness of 0.3-1mm, the braid angle of the three-dimensional braided structure is
45-60°。
Further, the three-dimensional braided structure be take axis yarn, boiled silk as the composite braided molding of knitting yarn of magnesium wire
Three-dimensional braided structure conduit skeleton.
Further, the diameter of the magnesium wire is 0.1-0.25mm, and radical is 4-16 root, between adjacent two magnesium wires between
Away from being 200-2000 μm, the line density of the boiled silk is 20-120D.
Further, titanium, nickel alloy wire are symmetrically arranged in the knitting yarn.
Further, the diameter of the nerve trachea internal layer is 1.8-5mm.
Further, the porous gel structure is Silk fibroin gel, schwann cell and nerve growth factor composition
Plural gel layer, the Silk fibroin gel freeze to form reticular fibre, and the schwann cell and nerve growth factor are set to
In the reticular fibre.
Further, the diameter of the reticular fibre is 10-1000nm, and aperture is 10-500 μm.
The utility model provides a kind of magnesium wire silk composite braided structures nerve trachea, its advantage is that:
(1) mechanical performances such as radial resistance to compression and the stretching of conduit are increased, while increasing the flexible curved of nerve trachea
Performance more adapts to complicated internal force environment;
(2) conduit biocompatibility, surface-active and permeability are good, can promote neuronal cell adherency, growth and
Proliferation, accelerates the reproduction speed of injured nerve;
(3) degradation rate of conduit can be regulated and controled, thus for the regeneration of nerve by selecting different material and modified technique
Suitable growing space is provided;
(4) since the magnesium wire in conduit is conductive, the magnesium generated when can stimulate and guide nerve growth, while degrade from
Son helps to improve the activity of neuronal cell.
Detailed description of the invention
It, below will be to required in embodiment description in order to illustrate more clearly of the technical solution of the utility model embodiment
The attached drawing used is briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the utility model
Example, for those of ordinary skill in the art, without any creative labor, can also be according to these attached drawings
Obtain other attached drawings.Wherein,
Fig. 1 is a kind of knot of magnesium wire silk composite braided structures nerve trachea described in the utility model in embodiment 1
Structure schematic diagram；
Fig. 2 is a kind of knot of magnesium wire silk composite braided structures nerve trachea described in the utility model in example 2
Structure schematic diagram；
Fig. 3 is a kind of knot of magnesium wire silk composite braided structures nerve trachea described in the utility model in embodiment 1
Structure schematic diagram；
Fig. 4 be the utility model embodiment 1 described in using different parameters preparation internal diameter be respectively 2 mm and 3 mm magnesium
The braid angle of silk silk composite braided structures nerve trachea；
Fig. 5 be the utility model embodiment 1 described in using different parameters preparation internal diameter be respectively 2 mm and 3 mm magnesium
Load-elongation strain curve of silk silk composite braided structures nerve trachea.
Wherein: 1 being nerve trachea outer layer, 2 be nerve trachea middle layer, 3 be nerve trachea internal layer, 4 be axis yarn, 5 be volume
Weaving yarn, 6 be Silk fibroin gel, 7 be schwann cell, 8 be nerve growth factor, 9 be titanium alloy wire.
Specific embodiment
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below with reference to specific implementation
Mode and attached drawing are described in further detail the utility model.
The utility model provides a kind of magnesium wire silk composite braided structures nerve trachea, as shown in Figure 1, the conduit is divided into three
Layer, comprising: nerve trachea outer layer 1 is the mandruka structure that is formed after silk fibroin protein solution and chitosan solution mixing, thickness
For 0.5-1.5mm, aperture is at 20-150 μm, and for porosity in 75%-90%, nerve trachea middle layer 2 is three-dimensional braided structure, can be with
It is any in three kinds of diamond weave (1/1 interweaves), rule braiding (2/2 interweaves) and He Gelisi (3/3 interweaves) braiding structures
A kind of braiding structure, thickness is in 0.3-1mm, and braid angle is 45-60 °, and nerve trachea internal layer 3 is porous gel structure, and diameter is
1.8-5mm, degradation time are 1-5 days.Nerve trachea middle layer 2 be by magnesium wire be axis yarn 4, a diameter of 0.1-0.25mm,
Radical is 4-16 root, and the spacing between magnesium wire is 200-2000 μm, and boiled silk is knitting yarn 5, line density 20-120D,
Composite braided molding three-dimensional structure conduit skeleton；Nerve trachea internal layer 3 is by Silk fibroin gel 6, schwann cell 7 and nerve
The plural gel layer that growth factor 8 forms has the effect of guiding nerve cell oriented growth.Nerve growth factor 8 is nerve
Member growth and necessary protein molecule of surviving, the reticular fibre diameter that freeze-drying is formed is 10-1000nm, and aperture exists
10-500μm.Guide-tube structure has excellent flexibility and bendability, can be well adapted for the force environment of internal complexity.
The preparation method of above-mentioned magnesium wire silk composite braided structures nerve trachea the following steps are included:
(1) nerve trachea middle layer 2 is prepared, nerve trachea skeleton knot is prepared using the vertical ingot formula braider of belt carcass function
Structure, magnesium wire are axis yarn 4, and boiled silk is knitting yarn 5, composite braided molding.
(2) preparation of silk fibroin protein solution: the Na that 30g silkworm raw silk boils in 12L is weighed2CO3(0.02 M) aqueous solution
Middle degumming 30 minutes is stirred with glass bar, to guarantee the completeness of degumming, then spends and repeatedly rush from water for every 10 minutes
It washes, washed fibroin fiber is placed in draught cupboard natural air drying overnight.25 g bombyx mori silk fibroin fibers after weighing drying
It is dissolved in 100 mL LiBr(9.3M) in solution, dissolved 4 hours in 60 DEG C of baking oven, slight wobble is per hour to guarantee to dissolve
Completely, the first mixed solution is obtained.It takes out the first mixed solution to pour into bag filter (molecular cut off 3500D), in deionization
It dialyses 36 hours in water, changes a water within every 4 hours.Solution after dialysis is in supercentrifuge with 9000 r/min centrifugation two
It is secondary, 20 minutes every time, to remove impurity, silk fibroin protein solution is finally obtained, is placed in 4 DEG C of refrigerators and places for use.
(3) it prepares nerve trachea outer layer 1: weighing the chitosan that 2-8g deacetylation is 80-95%, be dissolved in the vinegar of 0.5-4%
It in acid solution, stirs 4-12 hours, the chitosan solution that mass percent is 1-5% is made.According to silk fibroin protein solution: shell is poly-
Sugar juice=1:1 ~ 1:5, silk fibroin solution is slowly added into the chitosan solution prepared, is stirred 6-12 hours, is obtained
Second mixed solution.Boiled silk braiding layer (nerve trachea middle layer 2) is fixed in a mold, then the second mixed solution is poured
Enter in mold, be then placed in -20 DEG C of refrigerator freezings 4-12 hours, places into freeze drier after being freeze-dried 12-36 hours
It takes out, obtains the sponge cladding material with porous structure.
(4) nerve trachea internal layer 3 is prepared:
1) concentration is that 0.01-0.5% culture has the silk fibroin protein solution of schwann cell to inject by the conduit after sealing freezing
Into conduit hollow structure, is placed 12 hours at 37 DEG C and induce its gelation；2) conduit is perpendicularly inserted into liquid nitrogen container, edge is led
Pipe is axially slowly freezed downwards with the speed of 0.1-1 mm/s, is placed into freeze drier and is freeze-dried 24-48 hours.
It is with reference to the accompanying drawing and real to keep the above objects, features, and advantages of the utility model more obvious and easy to understand
It applies example and further illustrates the technical solution of the utility model.But the utility model is not limited to listed embodiment, should also wrap
Include other any well known changes in the interest field required by the utility model.
Firstly, " one embodiment " or " embodiment " referred to herein, which refers to, may be included at least one reality of the utility model
A particular feature, structure, or characteristic in existing mode." in one embodiment " that different places occur in the present specification is not
The same embodiment is referred both to, nor the individual or selective embodiment mutually exclusive with other embodiments.
Secondly, the utility model is described in detail using structural schematic diagram etc., when the utility model embodiment is described in detail,
For purposes of illustration only, schematic diagram, which can disobey general proportion, makees partial enlargement, and the schematic diagram is example, should not be limited herein
The range of the utility model protection processed.In addition, the three-dimensional space of length, width and depth should be included in actual fabrication.
Embodiment 1
The implementation case shows a kind of magnesium wire silk composite braided structures nerve trachea structure and preparation method, such as Fig. 1 institute
Show, which shares three layers, wherein the axis yarn 4 of nerve trachea middle layer 2 is magnesium wire, the braiding of nerve trachea middle layer 2
Yarn 5 is boiled silk, and nerve trachea outer layer 1 is the mandruka layer that fibroin albumen and chitosan solution are mixed, fibroin egg
White and chitosan mass ratio is 10:1, and chitosan solution concentration is 3-6 mg/mL, silk fibroin water solution concentration 5-10%(w/
V).It is porous fibroin gel layer made of 5-10%(w/v) silk fibroin water solution that nerve trachea internal layer 3, which is concentration,.Prepare work
Skill process is as follows:
(1) use weaving method to prepare nerve trachea middle layer 2: raw material exist for 20-100 D boiled silk, diameter
The magnesium wire of 0.05-0.2 mm, weaving use 8-32 ingot vertical braider, and diamond weave (1/1 interweaves), knitting angle is
45-60 °, gear ratio (gear wheel: pinion gear=80-120:18-60), revolving speed is set as 5-100 rpm.
(2) woven nerve trachea middle layer 2 is fixed in tubulose Teflon mould, then molten by what is prepared
Liquid pours into mold, and forming temperature is 60 ± 2 DEG C, molding time 15-30 min.It is put into -20 DEG C of refrigerator freezings 4-12 hours, then
It is put into after being taken out after being freeze-dried 12-36 hours in freeze drier, steam treatment, steam treatment 5-10 is carried out to conduit
Hour, treatment temperature is 50-70 DEG C, obtains the nerve trachea with mandruka layer.
(3) conduit after sealing freezing injects the silk fibroin protein solution that the concentration containing schwann cell is 0.01-0.5%
Into conduit hollow structure, is placed 12 hours at 37 DEG C and induce its gelation；Conduit is perpendicularly inserted into liquid nitrogen container again, edge is led
Pipe is axial slowly to carry out downwards liquid nitrogen frozen with the speed of 0.1-1mm/s, is freeze-dried finally, being placed in freeze drier
24-48 hours, obtain the silk extract gel layer containing schwann cell.
The performance of magnesium wire silk composite braided structures nerve trachea obtained by the above method please refers to Fig. 4-Fig. 5, such as Fig. 4
It is found that the braid angle of magnesium wire silk composite braided structures nerve trachea changes between 40-55 °, braid angle is stablized；As Fig. 5 can
Know, first peak indicates that knitting yarn is broken peak, and elongation strain at this time is 8%, and load is up to 35N, and second peak indicates the disconnected of axis yarn
Peak is split, load is greater than 65N at this time, shows that the addition of axis yarn greatly strengthens the tensile property of nerve trachea.
Embodiment 2
The implementation case show a kind of magnesium wire silk composite braided structures nerve trachea structure and preparation method, such as Fig. 2
Shown, which shares three layers, wherein the axis yarn 4 of nerve trachea middle layer 2 is magnesium wire, is symmetrically added four in knitting yarn 5
For the degradable titanium alloy wire 9 of root to enhance the radial crushing strength of nerve trachea, it is 3-6 that nerve trachea outer layer 1, which is by concentration,
Mandruka layer made of the chitosan solution of mg/mL, solution concentration are 3-6 mg/mL, and nerve trachea internal layer 3 is by concentration
For silk extract gel made of 5-10%(w/v) silk fibroin water solution.Preparation process flow is as follows:
(1) use weaving method to prepare nerve trachea middle layer 2: raw material exist for 20-100 D boiled silk, diameter
The magnesium wire and titanium alloy wire of 0.05-0.2 mm, weaving use 8-32 ingot vertical braider, and rule weaves (2/2 interweaves), on
Machine braid angle is 45-60 °, and gear ratio (gear wheel: pinion gear=80-120:18-60), revolving speed is set as 5-100 rpm.
(2) nerve trachea middle layer is fixed in tubulose Teflon mould, then the solution prepared is poured into mould
In tool, forming temperature is 60 ± 2 DEG C, molding time 15-30 min.It is put into -20 DEG C of refrigerator freezings 4-12 hours, then is placed in freezing
After being taken out after being freeze-dried 12-36 hours in drying machine, steam treatment is carried out to conduit, steam treatment 5-10 hours, is located
Managing temperature is 50-70 DEG C, obtains the mandruka layer of nerve trachea.
(3) conduit after sealing freezing injects the silk fibroin protein solution that the concentration containing schwann cell is 0.01-0.5%
Into conduit hollow structure, is placed 12 hours at 37 DEG C and induce its gelation；Conduit is perpendicularly inserted into liquid nitrogen container again, edge is led
Pipe is axially slowly freezed downwards with the speed of 0.1-1mm/s, finally, being placed in freeze drier carries out freeze-drying 24-48
Hour, obtain the silk extract gel layer containing schwann cell.
Embodiment 3
The implementation case show a kind of magnesium wire silk composite braided structures nerve trachea structure and preparation method, such as Fig. 3
Shown, which shares three layers, wherein the axis yarn 4 of nerve trachea middle layer 2 is that magnesium wire and boiled silk interweave, braiding
Yarn 5 is boiled silk, and nerve trachea outer layer 1 is the mandruka by concentration for the silk fibroin water solution preparation of 5-10%(w/v)
Layer；It is porous fibroin gel layer made of 5-10%(w/v) silk fibroin water solution that nerve trachea internal layer 3, which is concentration,.Prepare work
Skill process is as follows:
(1) use weaving method to prepare nerve trachea middle layer 2: raw material are for 20-100 D boiled silk, diameter
The magnesium wire of 0.05-0.2 mm, weaving use 8-32 ingot vertical braider, and He Gelisi (3/3 interweaves), knitting angle is
45-60 °, gear ratio (gear wheel: pinion gear=80-120:18-60), revolving speed is set as 5-100 rpm.
(2) nerve trachea middle layer is fixed in tubulose Teflon mould, then the solution prepared is poured into mould
In tool, forming temperature is 60 ± 2 DEG C, molding time 15-30 min.It is put into -20 DEG C of refrigerator freezings 4-12 hours, then is placed in freezing
After being taken out after being freeze-dried 12-36 hours in drying machine, conduit is carried out steam treatment 5-10 hours, treatment temperature 50-
70 DEG C, obtain the nerve trachea that outer layer 1 is mandruka layer.
(3) conduit after sealing freezing injects the silk fibroin protein solution that the concentration containing schwann cell is 0.01-0.5%
Into conduit hollow structure, is placed 12 hours at 37 DEG C and induce its gelation；Conduit is perpendicularly inserted into liquid nitrogen container again, edge is led
Pipe axially is slowly freezed downwards with the speed of 0.1-1 mm/s, finally, be placed in refrigerator carry out freeze-drying 24-48 it is small
When, obtain the silk extract gel layer containing schwann cell.
In conclusion the utility model discloses a kind of magnesium wire silk composite braided structures nerve trachea, using have compared with
The medical metal magnesium wire of strong mechanical property is that axis yarn is added in silk braiding structure, not only plays the role of longitudinal guide, but also can mention
The radial crush resistance energy of height braiding skeleton, meanwhile, magnesium wire can slowly degrade, and promote the growth and proliferation of schwann cell, be conducive to
The recovery of injuring nerve；Braiding yarn is boiled silk, has good biocompatibility, is guaranteeing the same of good mechanical properties
When, generate the biological microenvironment for promoting nerve regneration.
It should be noted that above embodiments are merely intended for describing the technical solutions of the present application, but not for limiting the present application, although reference
The utility model is described in detail in preferred embodiment, those skilled in the art should understand that, it can be to this reality
It is modified or replaced equivalently with novel technical solution, without departing from the spirit and scope of the technical scheme of the present invention,
It should all cover in the scope of the claims of the utility model.
Claims (8)
1. a kind of magnesium wire silk composite braided structures nerve trachea, it is characterised in that: including in nerve trachea outer layer, nerve trachea
Interbed and nerve trachea internal layer, the nerve trachea middle layer are coated on the outside of the nerve trachea internal layer, and the nerve is led
Outer tube layer is coated on the outside of the nerve trachea middle layer, and the nerve trachea outer layer is mandruka structure, the nerve
Conduit middle layer is three-dimensional braided structure, and the nerve trachea internal layer is porous gel structure.
2. magnesium wire silk composite braided structures nerve trachea according to claim 1, it is characterised in that: the nerve trachea
Outer layer with a thickness of 0.5-1.5mm, aperture is 20-150 μm, porosity 75%-90%.
3. magnesium wire silk composite braided structures nerve trachea according to claim 1, it is characterised in that: the 3 D weaving
Structure is any one in diamond weave structure, regular braiding structure and He Gelisi braiding structure.
4. magnesium wire silk composite braided structures nerve trachea according to claim 1, it is characterised in that: the nerve trachea
Middle layer with a thickness of 0.3-1mm, the braid angle of the three-dimensional braided structure is 45-60 °.
5. magnesium wire silk composite braided structures nerve trachea according to claim 1, it is characterised in that: the 3 D weaving
It is the composite braided molding three-dimensional structure conduit skeleton of knitting yarn that structure, which is by axis yarn, boiled silk of magnesium wire,.
6. magnesium wire silk composite braided structures nerve trachea according to claim 5, it is characterised in that: the magnesium wire it is straight
Diameter is 0.1-0.25mm, and radical is 4-16 root, and the spacing between adjacent two magnesium wires is 200-2000 μm, the boiled silk
Line density is 20-120D.
7. magnesium wire silk composite braided structures nerve trachea according to claim 5, it is characterised in that: in the knitting yarn
It is symmetrically arranged with titanium, nickel alloy wire.
8. magnesium wire silk composite braided structures nerve trachea according to claim 1, it is characterised in that: the nerve trachea
The diameter of internal layer is 1.8-5mm.
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