CN209559816U - PAGE gel is loaded swimming lane shaping jig - Google Patents
PAGE gel is loaded swimming lane shaping jig Download PDFInfo
- Publication number
- CN209559816U CN209559816U CN201920254346.2U CN201920254346U CN209559816U CN 209559816 U CN209559816 U CN 209559816U CN 201920254346 U CN201920254346 U CN 201920254346U CN 209559816 U CN209559816 U CN 209559816U
- Authority
- CN
- China
- Prior art keywords
- vertical plate
- plate
- forming part
- transversely mounted
- swimming lane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model provides a kind of PAGE gel sample-adding swimming lane shaping jig, belongs to the technical field of gel electrophoresis, including the first forming part and the second forming part；First forming part is transversely mounted plate and multiple first vertical plates including first；First is transversely mounted on plate and is equidistantly provided with multiple first square holes；The top setting of first vertical plate in, is provided with the card slot of recess in the first rectangular hole edge on inner sidewall；Second forming part is transversely mounted plate and gear shaping including second；Second is transversely mounted on plate and is provided with the second square hole；Gear shaping includes the second vertical plate, third vertical plate and the 4th vertical plate and bottom plate that the second rectangular hole edge is arranged in outside；Second vertical plate is opposite with the first vertical plate, and third vertical plate and the 4th vertical plate are located at the second vertical plate two sides, and are provided with fixture block corresponding with card slot in third vertical plate and the 4th vertical plate.After the tool, sample is not easy to be added to except sample-adding swimming lane, and rifle needle is also not easy to puncture swimming lane.
Description
Technical field
The utility model belongs to the technical field of gel electrophoresis, more particularly to a kind of PAGE gel sample-adding swimming lane at
Type tool.
Background technique
Western blot(immunoblotting) experiment in, prepare SDS-PAGE(dodecyl sodium sulfate-polyacrylamide
Amine) gel when generally comprise following step: firstly, by the alignment of two pieces of glass plate bottoms and fitting together, deposit therebetween
It is about 1-1.5 millimeters in gap；Then, a certain amount of separation gel is poured into gap, after its completely solidification, then will be dense
Contracting glue pours into and the gap top of going directly, and comb is inserted into concentration glue at once, obtains glass plastic plate.When concentration glue is completely solidifying
Gu after, after pulling out comb, sample-adding swimming lane is revealed.By glass plastic plate integral installation in electrophoresis tank, existed by rifle needle
Electrophoresis experiment is carried out after adding 20-30 microlitres of sample in sample-adding swimming lane.During the experiment, experimenter has found to exist and ask as follows
Topic: 1, after pulling out comb, sample-adding swimming lane does not see Chu, and sample-adding is easy to be added to except sample-adding swimming lane；2, rifle needle may pierce when being loaded
Broken swimming lane, sample are added in concentration glue；3, when being loaded, it is excessive that rifle needle withstands glass plate strength, separates glass plate, prepares
Glue is destroyed.
Utility model content
The purpose of this utility model is to provide a kind of PAGE gels to be loaded swimming lane shaping jig, to improve above-mentioned ask
Topic.
To achieve the above object, the utility model provides a kind of PAGE gel sample-adding swimming lane shaping jig, including the
One forming part and the second forming part；First forming part is transversely mounted plate and multiple first vertical plates including first；First laterally pacifies
Multiple first square holes are equidistantly provided in loading board；The top setting of first vertical plate is in the first rectangular hole edge edge, inside
The card slot of recess is provided on wall；Second forming part is transversely mounted plate including second and corresponds with first vertical plate
Gear shaping；Second is transversely mounted on plate and is provided with and one-to-one second square hole of first square hole；Gear shaping includes setting
Set the second vertical plate, third vertical plate and the 4th vertical plate in the second rectangular hole edge outside and setting the second vertical plate,
The bottom plate of third vertical plate and the 4th vertical plate bottom edge；Second vertical plate is opposite with the first vertical plate, third vertical plate and the 4th
Vertical plate is located at the second vertical plate two sides, and is provided with card corresponding with card slot in third vertical plate and the 4th vertical plate
Block；Gear shaping is inserted into the first vertical plate along card slot, and bottom plate is resisted against in the first vertical plate, the first vertical plate, the second vertical plate, the
Three vertical plates, the 4th vertical plate and bottom plate enclose open-topped cuboid cavity.
Further, the first forming part and the second forming part are integrally formed using medical plastic.
Further, first be transversely mounted plate and second be transversely mounted plate side rotation be provided with lifting ring；Lift ring
Plate is transversely mounted close to first or second is transversely mounted the end of plate.
The beneficial effects of the utility model are:
When being loaded swimming lane shaping jig using above-mentioned PAGE gel, first the gear shaping of the second forming part is inserted along card slot
Enter the first vertical plate, bottom plate is resisted against in the first vertical plate, and the first vertical plate, the second vertical plate, third vertical plate, the 4th are vertically
Plate and bottom plate enclose open-topped cuboid cavity, and the first forming part and the second forming part form comb, according to conventional side
Comb is inserted into concentration glue by formula, after glue to be concentrated solidifies completely, pulls out the second forming part, the first vertical plate of the first forming part
It stays in sample-adding swimming lane, sees sample-adding swimming lane clearly convenient for experimenter, when sample-adding, rifle needle withstands card slot, makes sample along card slot stream
Into sample-adding swimming lane, sample is not easy to be added to except sample-adding swimming lane, and rifle needle is also not easy to puncture swimming lane, since rifle needle is without withstanding glass
Plate avoids rifle aiming at the problem that glass plate applies active force and crosses the separation of ambassador's glass plate.After the completion of sample-adding, the first molding is pulled out
Portion can carry out electrophoresis experiment.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that PAGE gel provided by the embodiment of the utility model is loaded swimming lane shaping jig；
Fig. 2 is the installation diagram that PAGE gel shown in Fig. 1 is loaded swimming lane shaping jig；
Fig. 3 is the top view that PAGE gel shown in Fig. 2 is loaded swimming lane shaping jig；
Fig. 4 is the main view that PAGE gel shown in Fig. 1 is loaded the first forming part in swimming lane shaping jig；
Fig. 5 is the top view of the first forming part shown in Fig. 4；
Fig. 6 is the enlarged drawing of part A in Fig. 5；
Fig. 7 is the main view that PAGE gel shown in Fig. 1 is loaded the second forming part in swimming lane shaping jig；
Fig. 8 is the top view of the second forming part shown in Fig. 7；
Fig. 9 is the enlarged drawing of part B in Fig. 8.
In figure: the first forming part of 1-；1.1- first is transversely mounted plate；The first vertical plate of 1.2-；The first square hole of 1.3-；
1.4- card slot；The second forming part of 2-；2.1- second is transversely mounted plate；The second square hole of 2.2-；The second vertical plate of 2.3-；2.4- the
Three vertical plates；The 4th vertical plate of 2.5-；2.6- bottom plate；2.7- fixture block.
Specific embodiment
The present embodiment provides a kind of PAGE gels to be loaded swimming lane shaping jig, including the first forming part 1 and the second one-tenth
Type portion 2；First forming part 1 is transversely mounted plate 1.1 and multiple first vertical plates 1.2 including first；First is transversely mounted on plate 1.1
Spaced set has multiple first square holes 1.3；The top of first vertical plate 1.2 is arranged in 1.3 edge of the first square hole, interior
The card slot 1.4 of recess is provided on side wall；Second forming part 2 include second be transversely mounted plate 2.1 and with the first vertical plate 1.2
One-to-one gear shaping；Second is transversely mounted on plate 2.1 and is provided with and one-to-one second square hole of the first square hole 1.3
2.2；Gear shaping includes that the second vertical plate 2.3 being arranged in outside 2.2 edge of the second square hole, third vertical plate 2.4 and the 4th are vertical
Plate 2.5 and the bottom plate 2.6 that the second vertical plate 2.3,2.5 bottom edge of third vertical plate 2.4 and the 4th vertical plate are set；Second is perpendicular
Opposite to plate 2.3 and the first vertical plate 1.2, third vertical plate 2.4 and the 4th vertical plate 2.5 are located at the second vertical plate 2.3
Two sides, and fixture block 2.7 corresponding with card slot 1.4 is provided in third vertical plate 2.4 and the 4th vertical plate 2.5；Gear shaping is along card
Slot 1.4 is inserted into the first vertical plate 1.2, and bottom plate 2.6 is resisted against in the first vertical plate 1.2, the first vertical plate 1.2, the second vertical plate
2.3, third vertical plate 2.4, the 4th vertical plate 2.5 and bottom plate 2.6 enclose open-topped cuboid cavity.
When being loaded swimming lane shaping jig using above-mentioned PAGE gel, first by the gear shaping of the second forming part 2 along card slot
1.4 the first vertical plates 1.2 of insertion, bottom plate 2.6 are resisted against in the first vertical plate 1.2, the first vertical plate 1.2, the second vertical plate
2.3, third vertical plate 2.4, the 4th vertical plate 2.5 and bottom plate 2.6 enclose open-topped cuboid cavity, the first forming part
1 and second forming part 2 form comb, in a conventional manner by comb be inserted into concentration glue in, after glue to be concentrated solidifies completely, pull out
Second forming part 2, the first vertical plate 1.2 of the first forming part 1 stay in sample-adding swimming lane, see sample-adding swimming clearly convenient for experimenter
Road, when sample-adding, rifle needle withstands card slot 1.4, flows to sample in sample-adding swimming lane along card slot 1.4, and sample is not easy to be added to sample-adding swimming
Except road, rifle needle is also not easy to puncture swimming lane, since rifle needle is without withstanding glass plate, avoids rifle for glass plate and applies active force
The problem of crossing the separation of ambassador's glass plate.After the completion of sample-adding, electrophoresis experiment can be carried out by pulling out the first forming part 1.
Further, since sample-adding amount only has 20-30 microlitres, in order to avoid waste, guarantee sample-adding accuracy, the first molding
Portion 1 and the second forming part 2 are integrally formed using medical plastic, plastics not with albumen qualitative response, to the adhesiveness of protein also compared with
It is small.
Further, first be transversely mounted plate 1.1 and second be transversely mounted plate 2.1 side rotation be provided with lifting ring 3；
It lifts ring 3 and is transversely mounted the end that plate 1.1 or the second is transversely mounted plate 2.1 close to first.It can be by first by drawing lifting ring 3
Forming part 1 and the second forming part 2 are pulled out, and are lifted ring 3 and be rotatably arranged on first and be transversely mounted plate 1.1 and second and be transversely mounted plate
2.1 side will not influence the first forming part 1 and the assembly of the second forming part 2.
The above is only the embodiments of the present invention, and therefore it does not limit the scope of the patent of the utility model, all utilizations
Equivalent structure or equivalent flow shift made by the utility model description is applied directly or indirectly in other relevant
Technical field is also included in the patent protection scope of the utility model.
Claims (3)
1. a kind of PAGE gel is loaded swimming lane shaping jig, which is characterized in that including the first forming part and the second forming part；
First forming part is transversely mounted plate and multiple first vertical plates including first；
Described first is transversely mounted on plate and is equidistantly provided with multiple first square holes；
The top setting of first vertical plate in, is provided with the card slot of recess in the first rectangular hole edge on inner sidewall；
Second forming part include second be transversely mounted plate and with the one-to-one gear shaping of the first vertical plate；
Described second is transversely mounted on plate and is provided with and one-to-one second square hole of first square hole；
The gear shaping include the second vertical plate, third vertical plate and the 4th vertical plate being arranged in the second rectangular hole edge outside and
The bottom plate of second vertical plate, third vertical plate and the 4th vertical plate bottom edge is set；
Second vertical plate is opposite with the first vertical plate, and third vertical plate and the 4th vertical plate are located at the second vertical plate two
Side, and fixture block corresponding with card slot is provided in third vertical plate and the 4th vertical plate；
The gear shaping is inserted into the first vertical plate along card slot, and bottom plate is resisted against in the first vertical plate, and the first vertical plate, second are vertically
Plate, third vertical plate, the 4th vertical plate and bottom plate enclose open-topped cuboid cavity.
2. PAGE gel according to claim 1 is loaded swimming lane shaping jig, which is characterized in that first molding
Portion and the second forming part are integrally formed using medical plastic.
3. PAGE gel according to claim 1 is loaded swimming lane shaping jig, which is characterized in that described first laterally
Mounting plate and second be transversely mounted plate side rotation be provided with lifting ring；
The lifting ring is transversely mounted plate close to first or second is transversely mounted the end of plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920254346.2U CN209559816U (en) | 2019-02-28 | 2019-02-28 | PAGE gel is loaded swimming lane shaping jig |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920254346.2U CN209559816U (en) | 2019-02-28 | 2019-02-28 | PAGE gel is loaded swimming lane shaping jig |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209559816U true CN209559816U (en) | 2019-10-29 |
Family
ID=68311225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920254346.2U Expired - Fee Related CN209559816U (en) | 2019-02-28 | 2019-02-28 | PAGE gel is loaded swimming lane shaping jig |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209559816U (en) |
-
2019
- 2019-02-28 CN CN201920254346.2U patent/CN209559816U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191029 |