CN209559811U - A kind of multi-parameter electrochemical detection electrode piece - Google Patents
A kind of multi-parameter electrochemical detection electrode piece Download PDFInfo
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- CN209559811U CN209559811U CN201821983716.8U CN201821983716U CN209559811U CN 209559811 U CN209559811 U CN 209559811U CN 201821983716 U CN201821983716 U CN 201821983716U CN 209559811 U CN209559811 U CN 209559811U
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Abstract
The utility model provide it is a kind of it is easy to operate, prepare liquid can be prevented contaminated, and compact-sized multi-parameter electrochemical detection electrode piece.The electrode slice, including substrate, reference electrode and multiple working electrodes, each working electrode have respective syphon runner, and electrode slice further includes pipette, and pipette is fixedly connected with substrate, and pipette is connected to multiple syphon runners.Prepare liquid is sucked into pipette, by syphon runner is guided to reference electrode and each working electrode again.Syphon runner has siphon port, and siphon port is located at the long side of substrate, and pipette is fixedly connected on the substrate long side, and pipette is connected to by siphon port with multiple syphon runners.
Description
Technical field
The utility model relates to field of electrochemical detection, and in particular to a kind of multi-parameter electrochemical detection electrode piece.
Background technique
Following background technique is used to help reader and understands the utility model, and is not construed as the prior art.
It is widely used using electrochemica biological sensor as the screen printing electrode of principle in detection field (POCT) immediately.It is raw
Liquid is managed, as contained the interested detection parameters of many clinical labororatories in blood, urine, sweat, saliva.Such as in blood
Glucose, cholesterol, glycosylated hemoglobin, blood ketone, the uric acid in urine, the lactic acid etc. in sweat.The testing principle of electrode is
By on enzyme modification to working electrode corresponding to target analytes, pass through the enzymatic reaction electric current realization pair of detection target analytes
The fast quantitative analysis of target analytes.
Detection while in order to realize multi-parameter, Chinese patent application CN103048462A disclose a kind of based on electrode array
The multi-parameter electrochemical immunosensor of column, the multi-parameter electrochemical immunosensor have detecting electrode array, each detection
Electrode have it is matched to electrode and reference electrode, using more set detecting electrodes, to electrode and reference electrode come to multiple tumours
The content of marker is detected.When detection, sensor electrode is placed in prepare liquid and is immunoreacted.The patent application
Disadvantage is: 1, all electrodes could be infiltrated by needing to collect more prepare liquid；2, prepare liquid is conductive, in the detection process
Electric signal between each detecting electrode is easy to interfere with each other by prepare liquid conduction, influences the accuracy of detection；3, structure is not
It is enough compact, when needing detection parameters to increase, it is necessary to widen the size of electrode slice, to accommodate more detecting electrodes.At other
In the prior art, it when carrying out multi-parameter detection, after sampling, needs to be added in sample drop using dropper or other liquid-transfering devices
Adding mouth, prepare liquid are split to each working electrode from adding mouth, generate electric signal.Sampling, transfer, the whole process being loaded
It is cumbersome, and in transfer process, prepare liquid is easily contaminated.
Utility model content
The purpose of this utility model is to provide it is a kind of it is easy to operate, prepare liquid can be prevented contaminated, and structure is tight
The multi-parameter electrochemical detection electrode piece to gather.
A kind of multi-parameter electrochemical detection electrode piece, including substrate, reference electrode and multiple working electrodes, feature exist
In: each working electrode has respective syphon runner, and the electrode slice further includes pipette, pipette and the fixed company of substrate
It connects, pipette is connected to multiple syphon runners.Prepare liquid be sucked into pipette, again by syphon runner be guided to reference electricity
Pole and each working electrode.
Further, syphon runner has siphon port, and siphon port is located at the long side of substrate, and pipette is fixedly connected on described
Substrate long side, pipette are connected to by siphon port with multiple syphon runners.Preferably, the axis of pipette is perpendicular to siphon jet
The axis in road.
Further, pipette is capillary, and imbibition pipe internal surface is coated with hydrophilic film.Preferably, pipette has and is used for
The suction nozzle in prepare liquid to pipette is sucked, suction nozzle is located at one end of pipette, sets absorbent wool in the other end of pipette.It is preferred that
, suction nozzle is flushed with substrate one end, alternatively, suction nozzle protrudes from substrate.
Further, multiple partitions are equipped in syphon runner, syphon runner is separated into multiple subflow roads by partition.Preferably,
Subflow road is parallel with the axis of syphon runner.
Further, multiple syphon runners connect a reference electrode jointly.That is, multiple working electrodes share one
Reference electrode.Preferably, reference electrode runs through all syphon runners, and reference electrode is vertical with the axis of syphon runner.
Further, reference electrode is with respect to working electrode close to siphon port.
Further, working electrode has a junction with syphon runner, the junction of multiple working electrodes and corresponding rainbow
The distance between suction inlet is equal.
Further, it is equipped in syphon runner when being flowed through for prepare liquid, issues the pressure sensor of electric signal and for connecing
When receiving electric signal, the signal lamp of flashing, for pressure sensor with respect to working electrode far from siphon port, signal lamp and pressure sensor are electric
Connection.When prepare liquid is by flowing into syphon runner, when flowing through working electrode, reaching pressure sensor, pressure sensor generates electricity
Signal, and electric signal is sent to signal lamp, signal lamp flashes after receiving electric signal, prompts prepare liquid electric with work
Pole contact.
Further, each working electrode is modified with the biological chemical reagent for detecting target analytes, multiple work electricity
Pole is modified with identical biological chemical reagent, alternatively, multiple working electrodes modify different biological chemical reagents.Multiple work electricity
Pole is modified different biological chemical reagents and be can be, and each working electrode is modified with different biological chemical reagents, work electricity
The species number that the quantity of pole is greater than or equal to biological chemical reagent is equal.
Further, biological chemical reagent is glucose oxidase, mutarotase, glucose dehydrogenase, lactate oxidase, uric acid
Oxidizing ferment, cholesterol oxidase, phosphatidase, aminoacidase, horseradish peroxidase or beta-hydroxybutyric dehydrogenase.
The utility model has the beneficial effects that
1, pipette directly draws prepare liquid, and prepare liquid is guided to working electrode by syphon runner, save sampling,
The step of transfer, drop sample, it is more convenient to operate, and prepare liquid is prevented to be contaminated in transfer process；Pipette is capillary
Pipe, a small amount of prepare liquid can form capillarity in pipette, it made to be guided to working electrode, fill prepare liquid
Divide and utilizes.
2, each working electrode has respective syphon runner, when detecting multiple parameters at the same time, on each working electrode
The electric signal of generation will not interfere with each other, and improve the accuracy of detection.
3, pressure sensor and signal lamp is equipped in syphon runner to keep away for prompting whether prepare liquid flows through working electrode
Exempt from detection error caused by syphon runner or imbibition blockage.
4, multiple parallel subflow roads are set in syphon runner, can be accelerated prepare liquid by syphon runner and be flow to working electrode
Speed.
5, multiple siphon ports are respectively positioned on the same long side of substrate, and substrate long side size is larger, can tie up in substrate
While holding original size, increase working electrode and syphon runner quantity, keep structure more compact, saves space, saves material.
6, it is connect due to syphon runner with the same reference electrode, that is, multiple working electrodes share a reference electricity
Material is saved in pole；In addition, reference electrode with respect to working electrode close to siphon port, so that prepare liquid is passed through reference electrode first and flow again
To each working electrode, the enzymatic reaction on each working electrode is avoided to have an impact the potential of reference electrode, improves detection
Accuracy.
Detailed description of the invention
Fig. 1 is the appearance diagram of multi-parameter electrochemical detection electrode piece in the utility model one embodiment.
Fig. 2 is the schematic diagram of electrode layer in the utility model one embodiment.
Fig. 3 is the schematic diagram of guide layer in the utility model one embodiment.
Fig. 4 is the schematic diagram of the utility model one embodiment cover plate.
Specific embodiment
The utility model is described in further detail combined with specific embodiments below.
A kind of multi-parameter electrochemical detection electrode piece, as shown in Figure 1, including substrate, reference electrode 104 and multiple work electricity
Pole 101, working electrode 102 and working electrode 103, each working electrode have respective syphon runner 301, and the electrode slice is also
Including pipette 2, pipette 2 is fixedly connected with substrate, and pipette 2 is connected to multiple syphon runners 301.Prepare liquid is sucked into
Pipette by syphon runner is guided to reference electrode and each working electrode again.Pipette can be by plastics, glass, gold
Belong to or alloy is made.As shown in Figures 2 and 3, substrate includes the electrode layer 1 and guide layer 3 mutually to splice, 104 He of reference electrode
Working electrode 101, working electrode 102 and working electrode 103 are set to electrode layer 1；Guide layer 3 is equipped with notch, notch and electrode
Syphon runner is collectively formed in layer 1.As shown in figure 4, electrode slice further includes cover board 4, guide layer 3 be located at cover board 4 and electrode layer 1 it
Between.Cover board 4 is located at 3 upper surface of guide layer, electrode layer 1 is located at 3 lower surface of guide layer.
As shown in Figure 1, syphon runner 301 has siphon port 3011, siphon port 3011 is located at the long side of substrate, pipette
2 are fixedly connected on the substrate long side, and pipette 2 is connected to by siphon port 3011 with multiple syphon runners 301.Pipette 2
Axis perpendicular to syphon runner 301 axis.The long side size of substrate is bigger, can accommodate more syphon runners, can
In the case where guarantee electrode slice original size, the quantity of increase working electrode and syphon runner.
Pipette is capillary, and imbibition pipe internal surface is coated with hydrophilic film.As shown in Figure 1, pipette 2 has for sucking
Suction nozzle 201 in prepare liquid to pipette, suction nozzle 201 are located at one end of pipette, set absorbent wool in the other end of pipette
202.The anti-Hemostatic Oral Liquid of absorbent wool is excessive, overflows from the other end of pipette.Pipette both ends are flushed with substrate long side both ends.It inhales
Mouth is flushed with substrate one end.
In some embodiments, suction nozzle can protrude from substrate.
Electrochemical detection electrode piece is usually used in detecting the content of target analytes in blood or urine.It is to detect blood
The suction nozzle of pipette is directed at drop of blood, drop of blood is under capillary action by water conservancy diversion with puncturing after device punctures skin, squeezes out drop of blood by example
To pipette.When blood flows through each siphon port, reference electrode and working electrode are guided to by syphon runner.Need how many
How much blood just squeezed out for detecting for blood, is avoided taking out extra blood and is caused to waste, but also without turning to blood
It moves, anti-Hemostatic Oral Liquid is contaminated, and improves accuracy in detection.
In some embodiments, multiple partitions are equipped in syphon runner, syphon runner is separated into multiple subflow roads by partition.
Subflow road is parallel with the axis of syphon runner.As shown in figure 3, partition 303 can be a part of guide layer 3, partition, which is located at, is led
Fluid layer notch is interior, vertical with notch axis, and partition makes notch in comb teeth-shaped.In some embodiments, the side of partition and electrode
Layer is fixedly connected, and the other side of partition is flushed with syphon runner upper surface.Syphon runner upper surface refers to syphon runner direction
The one side of cover board.When need working electrode and prepare liquid have biggish contact area, to increase electric signal when, need to increase siphon
The lateral dimension of runner can reduce prepare liquid in the flow velocity of syphon runner at this time, and the setting in subflow road can accelerate prepare liquid and exist
The flow velocity of syphon runner.In addition, being stamped cover board on syphon runner, partition can prevent cover board recessed under external force and rainbow
The fitting of runner bottom surface is inhaled, the blocking of syphon runner is caused.
In the present embodiment, all syphon runners share a cover board, and the cover board back side is equipped with hydrophilic film.The cover board back side refers to
Be cover plate lid when on substrate towards the one side of syphon runner.In some embodiments, cover board can have multiple, each siphon
Runner has respective cover board.
As shown in Figure 1, multiple syphon runners 301 connect a reference electrode 104 jointly.That is, multiple work electricity
Extremely share a reference electrode.Reference electrode 104 runs through all syphon runners 301, reference electrode 104 and syphon runner 301
Axis it is vertical.Reference electrode 104 is with respect to working electrode 101, working electrode 102 and working electrode 103 close to siphon port 3011.
In each syphon runner, prepare liquid is successively drained to reference electrode and working electrode.
Working electrode and syphon runner 301 have a junction, the junction of multiple working electrodes and corresponding siphon port 301
The distance between it is equal.As depicted in figs. 1 and 2, working electrode includes sequentially connected first segment, second segment and third section, and
One section parallel with third section, and second segment is vertical with first segment, third section, and third section and syphon runner have junction, third section
It is vertical with the axis of syphon runner.Each working electrode has corresponding working electrode terminal 105, and working electrode terminal 105 is used
In connection electrochemical workstation, electrochemical workstation is used to detect the electric signal of working electrode.The suction nozzle of pipette is back to work
Electrode terminal, working electrode terminal 105 are located at one end of substrate, and the suction nozzle 201 of pipette is flushed with the other end of substrate.
It is equipped in syphon runner 301 when being flowed through for prepare liquid, issues the pressure sensor 3021 of electric signal and for electricity
When signal, the signal lamp 3022 of flashing, pressure sensor 3021 with respect to working electrode far from siphon port 3011, signal lamp 3022 with
Pressure sensor 3021 is electrically connected.When prepare liquid is by flowing into syphon runner, when flowing through working electrode, reaching pressure sensor,
Pressure sensor generates electric signal, and electric signal is sent to signal lamp, and signal lamp flashes after receiving electric signal, prompt to
Survey liquid with working electrode contact.
Each working electrode is modified with the biological chemical reagent for detecting target analytes, and multiple working electrodes are modified with
Identical biological chemical reagent, alternatively, multiple working electrodes modify different biological chemical reagents.Biological chemical reagent is grape
Carbohydrate oxidase, mutarotase, glucose dehydrogenase, lactate oxidase, urate oxidase, cholesterol oxidase, phosphatidase, amino acid
Enzyme, horseradish peroxidase or beta-hydroxybutyric dehydrogenase.
When multiple working electrodes are modified with identical biological chemical reagent, it can be used for verifying the accuracy of testing result.
For example, modifying the Portugal for detecting Glucose in Blood by Cyclic on working electrode 101, working electrode 102 and working electrode 103
Grape carbohydrate oxidase, electrochemical workstation detect that the electric current of working electrode 101 is I1, working electrode 102 electric current be I2, work
The electric current of electrode 102 is I3, calculating current absolute value of the difference I1=|I1-I2|、∆I2=|I1-I3|、∆I3=|I2-I3|。∆I1、
∆I2And I3The correctness of testing result is represent, value is smaller, and testing result accuracy is higher.Pass through electric current I1、I2And I3
The concentration for calculating glucose in test sample can be corresponded to.
Multiple working electrodes are modified different biological chemical reagents and be can be, and each working electrode is modified with different lifes
Object chemical reagent, the quantity of working electrode are greater than or equal to the species number of biological chemical reagent.The quantity of working electrode is equal to life
The species number of object chemical reagent, each working electrode can be used for detecting a kind of substance, how many working electrode can detect
How many kinds of substance.For example, glucose oxidase, urine are modified on working electrode 101, working electrode 102 and working electrode 103 respectively
Acid oxidase and lactate oxidase, electrochemical workstation detect that the electric current of working electrode 101 is I4, working electrode 102 electricity
Stream is I5, working electrode 102 electric current be I6, pass through I4、I5With I6Value can calculate separately out glucose in test sample
The concentration of concentration, the concentration of uric acid and lactic acid.
In some embodiments, the quantity of working electrode is greater than the species number of biological chemical reagent.For example, one shares 6
Working electrode, working electrode A1、A2Upper modification glucose oxidase, for detecting the glucose content in prepare liquid, B1、B2、B3
Upper modification urate oxidase, for detecting the uric acid content in prepare liquid, C1Upper modification lactate oxidase, for detecting prepare liquid
Middle lactic acid content.
Content described in this specification embodiment is only an enumeration of the implementation forms of the utility model concept, this is practical new
The protection scope of type should not be construed as being limited to the specific forms stated in the embodiments, the protection scope of the utility model also and
In those skilled in the art according to the utility model design it is conceivable that equivalent technologies mean.
Claims (8)
1. a kind of multi-parameter electrochemical detection electrode piece, including substrate, reference electrode and multiple working electrodes, it is characterised in that:
Each working electrode has respective syphon runner, and the electrode slice further includes pipette, and pipette is fixedly connected with substrate, inhales
Liquid pipe is connected to multiple syphon runners.
2. multi-parameter electrochemical detection electrode piece as described in claim 1, it is characterised in that: syphon runner has siphon port,
Siphon port is located at the long side of substrate, and pipette is fixedly connected on the substrate long side, pipette by siphon port with it is multiple
Syphon runner connection.
3. multi-parameter electrochemical detection electrode piece as claimed in claim 2, it is characterised in that: pipette is capillary, imbibition
Pipe internal surface is coated with hydrophilic film.
4. multi-parameter electrochemical detection electrode piece as claimed in claim 3, it is characterised in that: pipette have for suck to
The suction nozzle in liquid to pipette is surveyed, suction nozzle is located at one end of pipette, sets absorbent wool in the other end of pipette.
5. multi-parameter electrochemical detection electrode piece as claimed in claim 4, it is characterised in that: be equipped in syphon runner it is multiple every
Syphon runner is separated into multiple subflow roads by plate, partition, and subflow road is parallel with the axis of syphon runner.
6. multi-parameter electrochemical detection electrode piece as claimed in claim 5, it is characterised in that: multiple syphon runners connect jointly
One reference electrode；Reference electrode is with respect to working electrode close to siphon port.
7. multi-parameter electrochemical detection electrode piece as claimed in claim 6, it is characterised in that: working electrode has and siphon jet
The junction in road, the junction of multiple working electrodes are equal with the distance between corresponding siphon port.
8. multi-parameter electrochemical detection electrode piece as claimed in claim 7, it is characterised in that: be equipped in syphon runner for
Survey liquid stream through when, issue the pressure sensor of electric signal and when for receiving electric signal, the signal lamp of flashing, pressure sensor phase
To working electrode far from siphon port, signal lamp is electrically connected with pressure sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201821983716.8U CN209559811U (en) | 2018-11-29 | 2018-11-29 | A kind of multi-parameter electrochemical detection electrode piece |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201821983716.8U CN209559811U (en) | 2018-11-29 | 2018-11-29 | A kind of multi-parameter electrochemical detection electrode piece |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146568A (en) * | 2018-11-29 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of multi-parameter electrochemical detection electrode piece |
| CN114839241A (en) * | 2022-02-28 | 2022-08-02 | 京东方科技集团股份有限公司 | Detection substrate, detection method thereof and detection device |
-
2018
- 2018-11-29 CN CN201821983716.8U patent/CN209559811U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146568A (en) * | 2018-11-29 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of multi-parameter electrochemical detection electrode piece |
| CN114839241A (en) * | 2022-02-28 | 2022-08-02 | 京东方科技集团股份有限公司 | Detection substrate, detection method thereof and detection device |
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