CN209555227U - The quantitative micro inoculator of Micro biological Tests - Google Patents

Abstract

The utility model discloses a kind of quantitative micro inoculators of Micro biological Tests comprising: its front end is for contacting the capillary to be tested with liquid；The overstriking pipe for being set to capillary tail end and being connected to the lumen of capillary；Being mounted on overstriking pipe is used to make overstriking pipe to generate deformation so that capillary draws liquid or the tubular change driving assembly of overstriking for the liquid drawn is discharged；Wherein, the outer diameter and inner diameter of the overstriking pipe is respectively greater than the outer diameter and inner diameter of the capillary；Wherein, the outer wall of the capillary is provided with the scale for identifying liquid capacity in it, and the capillary is transparent plastic tube, and the overstriking pipe is the transparent plastic flexible pipe that can produce deformation.The quantitative micro inoculator of the utility model, structure is simple, and manufacturing cost is low; no cross contamination, pipettes liquid capacity accuracy height, and manual operations is easy; it is suitable for that vast basic hospital laboratory routinely uses, it can be achieved that large-scale production, makes up the deficiency of existing operating method.

Description

The quantitative micro inoculator of Micro biological Tests

Technical field

The utility model relates to field of medical technology more particularly to a kind of quantitative micro inoculators of Micro biological Tests.

Background technique

Often it is related to the operation of micro, quantitative seed sample in clinical microbiology laboratory technique, such as urine specimen is inoculated in Culture medium carries out urine count of bacteria, or bronchoalveolar lavage fluid sample is inoculated in culture medium and carries out count of bacteria etc., according to quantitative Bacterium colony growth population after inoculation can converse the bacterial concentration in sample, with " Colony Forming Unit (Colony Forming Unit, cfu)/milliliter " it indicates, the bacterial concentration height in sample can distinguish pathogenic bacteria or contaminated bacteria and pathogenic bacteria Quantity is to aid in the important indicator of the urinary infection of clinician's diagnosis and differential diagnosis or lower respiratory tract infection.

It is currently used when needing to carry out count of bacteria to certain body fluid sample such as urine in clinical microbiology laboratory technique Way is to take urine (taking a ring amount of urine is about 10 microlitres) to be inoculated in culture medium with oese as shown in FIG. 6, grows bacterium colony It is converted into concentration (cfu/ml) counting again afterwards.However 10 microlitres of oese factory calibrations of import are error ± 20% at present, and are passed through After a period of time after use, surface tension is caused to change due to high-temperature sterilization and sample component residual etc., urine etc. connects Kind amount error can be even higher up to 40%.Inoculum concentration inaccuracy directly affects examining as a result, being particularly in for urine count of bacteria (10 when near the critical value of disconnected urinary infection⁴Cfu/ml, the bacterium less than this concentration are commonly referred to be during sample leaves and takes Contaminated bacteria caused by being not thorough because of the cleaning of Periurethral skin, without considering to be urinary infection), clinician can be interfered to secreting The diagnosing and treating of urine system infection.

Inoculation is replaced using the pipettor of 10 microlitres (μ l) adjustable ranges currently, there are also some conditions preferable laboratories Ring carries out quantitative inoculation, but 10 microlitres of pipettors are at high cost, and needs year calibration to ensure its accuracy, is not suitable for vast base Layer laboratory routinely uses.In addition, there are also using disposably with the suction pipe of sacculus, though have at low cost, no cross contamination Advantage, but existing Dispette range is not measured at least at 1 milliliter or more dedicated for urinating 10 microlitres of count of bacteria Journey, and the amount of liquid drawn is more rough, is unable to accurately control, and is also not used to urine count of bacteria.

Summary of the invention

The purpose of this utility model is exactly to solve the above-mentioned problems, to provide a kind of the quantitative of Micro biological Tests and micro connect Kind device.

For the above-mentioned purpose for realizing the utility model, the utility model provides a kind of the quantitative of Micro biological Tests and micro connects Kind device comprising: its front end is for contacting the capillary to be tested with liquid；It is set to capillary tail end and the pipe with capillary The overstriking pipe of chamber connection；Being mounted on overstriking pipe is used to make overstriking pipe to generate deformation so that capillary has drawn liquid or discharge The tubular change driving assembly of the overstriking of the liquid of absorption；Wherein, the outer diameter and inner diameter of the overstriking pipe is respectively greater than the capillary Outer diameter and inner diameter；Wherein, the outer wall of the capillary is provided with the scale for identifying liquid capacity in it, and the capillary Pipe is transparent plastic tube, and the overstriking pipe is the transparent plastic flexible pipe that can produce deformation.

Wherein, the tubular driving assembly that becomes of the overstriking includes: with the fixing seat for being set with the inner cavity of the overstriking pipe； The open slot for being opened in fixing seat side and being connected with its inner cavity；It is separately positioned on a pair of of relative inner of fixing seat open slot A pair of of card slot on wall；It is placed in open slot and its both ends is slidably connected by connecting shaft and a pair of of card slot so as to along fixing seat The mobile idler wheel of length extending direction；Wherein, the card slot extends along the length direction of the fixing seat；Wherein, described solid The both ends of reservation are separately provided for limiting the first limiting card and the second limiting card of the rolling stroke of idler wheel.

Wherein, first limiting card and second limiting card are located at the both ends of the open slot.

Wherein, the extended line of the card slot and the central axis of the fixing seat inner cavity intersect at a point.

Alternatively, the tubular driving assembly that becomes of the overstriking includes: with the fixing seat for being set with the inner cavity of the overstriking pipe； The open slot for being opened in fixing seat side and being connected with its inner cavity；Card on one inner sidewall of fixing seat open slot is set Slot；It is placed in open slot and one end is slidably connected to move along the length extending direction of fixing seat by connecting shaft and card slot The sliding block of the surface sliding for being located at another inner sidewall place of open slot side along fixing seat is arranged in dynamic idler wheel, the other end；Its In, the card slot extends along the length direction of the fixing seat；Wherein, the both ends of the fixing seat are separately provided for limiting The first limiting card and the second limiting card of the rolling stroke of idler wheel.

Wherein, the extended line of the card slot and the central axis of the fixing seat inner cavity intersect at a point.

Further, the tubular change driving assembly of the overstriking further includes being mounted on the idler wheel and one end is overlapped in institute State the passage frame on the surface of fixing seat.

Preferably, the internal diameter of the capillary is 0.5mm-1.5mm, and the internal diameter of the overstriking pipe is 8mm-12mm.

Compared with prior art, the quantitative micro inoculator of the Micro biological Tests of the utility model has the advantages that

The quantitative micro inoculator of the Micro biological Tests of the utility model, structure is simple, and manufacturing cost is low, and no intersection is dirty Dye pipettes liquid capacity accuracy height, and manual operations is easy, is suitable for that vast basic hospital laboratory routinely uses, it can be achieved that advising Modelling production, makes up the deficiency of existing operating method.

The utility model is described in detail with reference to the accompanying drawing.

Detailed description of the invention

Fig. 1 is that the quantitative micro inoculator of the utility model Micro biological Tests is driven using the tubular change of first embodiment overstriking The structural schematic diagram of dynamic component；

Fig. 2 is the structural schematic diagram of the utility model embodiment capillary Yu overstriking pipe；

Fig. 3 a is the tubular structural schematic diagram (not setting cue button) for becoming driving assembly of the utility model first embodiment overstriking；

Fig. 3 b is the tubular structural schematic diagram (setting cue button) for becoming driving assembly of the utility model first embodiment overstriking；

Fig. 4 is the tubular structural schematic diagram for becoming driving assembly of the utility model second embodiment overstriking；

Fig. 5 is the tubular structural schematic diagram for becoming driving assembly of the utility model 3rd embodiment overstriking；

Fig. 6 is the structure diagram of prior art oese.

Specific embodiment

As shown in Figure 1, the quantitative micro inoculator for the utility model Micro biological Tests uses first embodiment overstriking The tubular structural schematic diagram for becoming driving assembly, as shown in Figure 1, the quantitative micro inoculator packet of the Micro biological Tests of the present embodiment Include: its front end is for contacting the capillary 1 to be tested with liquid；It is set to 1 tail end of capillary and is connected to the lumen of capillary 1 Overstriking pipe 2；Being mounted on overstriking pipe 2 is used to make overstriking pipe 2 to generate deformation so that capillary 1 draws liquid or discharge has been inhaled The tubular change driving assembly 3 of the overstriking of the liquid taken；Wherein, the outer diameter and inner diameter of overstriking pipe 2 be respectively greater than capillary 1 outer diameter and Internal diameter；Wherein, the outer wall of capillary 1 is provided with the scale for identifying liquid capacity in it, and capillary 1 is transparent plastics Pipe, overstriking pipe 2 are the transparent plastic flexible pipe that can produce deformation.

Specifically, as shown in Fig. 2, 12 very little of internal diameter of capillary 1, and the outer wall of capillary 1 is provided with for indicating it The scale 11 of interior liquid capacity, when manufacture, capillary 1 is fabricated using transparent medical plastic hose.

It, will this be practical due to for the body fluid sample of inoculation being 10 microlitres when carrying out Micro biological Tests, therefore in design The maximum liquid capacity total amount that novel capillary 1 can be drawn is set as 20 microlitres, and the internal diameter of capillary can be taken as 1mm- 1.5mm, be computed can obtain corresponding length can for 25.5mm-11.3mm, and the wall thickness of plastic tube can determines according to actual conditions, It such as can be 1mm-6mm.

After the capillary 1 of the utility model draws liquid to be detected, for the liquid quantitative row convenient for drawing capillary 1 Out, that is, the liquid quantitative in capillary 1 is discharged 10 microlitres, tail end setting and capillary 1 of the utility model in capillary 1 The overstriking pipe 2 that is connected of lumen, with will pass through the tubular change driving assembly 3 of overstriking squeeze overstriking pipe 2, make capillary 1 draw to The liquid that detection liquid or discharge have been drawn.

The overstriking pipe 2 of the utility model is fabricated by the transparent plastic flexible pipe that can produce deformation, outer diameter and inner diameter The respectively greater than outer diameter and inner diameter of capillary 1.When manufacture, same material is can be used in the capillary 1 and overstriking pipe 2 of the utility model Expect that integrally formed method is fabricated, the material that prior art perfusion tube such as can be used is fabricated, and plastic flexible pipe has one Fixed elasticity, allows overstriking pipe to generate deformation under external force, such as under the action of overstriking tubular change driving assembly 3, adds Extra heavy pipe bore channels become larger or become smaller, so that the total measurement (volume) for the lumen that overstriking pipe 2 is connected to capillary 1 becomes larger or becomes smaller, thus It becomes smaller or larger the pressure in capillary 1 accordingly, and then draws or be discharged liquid.

When design, it is contemplated that convenient for disposing the tubular change driving assembly 3 of overstriking outside overstriking pipe 2 and being conveniently adjusted capillary In 1 the problem of pressure size, the internal diameter of the overstriking pipe 2 of the utility model can between 8mm-12mm, length 25mm-30mm it Between, wall thickness can be 1mm-6mm.

It wherein, is the volume convenient for manufacturing and drawing convenient for observation capillary 1 liquid, the internal diameter of the present embodiment capillary 1 It can be taken as 1.0mm, wall thickness 1mm, length 26mm, the internal diameter of overstriking pipe 2 can be taken as 8mm, wall thickness 1mm, length 25mm. It is of course also possible to determine the internal diameter of capillary and overstriking pipe, wall thickness and length according to actual operation requirements.

Wherein, the utility model makes overstriking pipe 2 generate deformation to change bore channels by the tubular change driving assembly 3 of overstriking Size, the tubular change driving assembly 3 of the overstriking can use structure as shown in Figure 3a, comprising: have for being set with overstriking pipe 2 Inner cavity fixing seat 31, cavity shape is adapted to overstriking pipe, and size does not generate original outer diameter phase when deformation with overstriking pipe When, that is, be slightly larger than or equal to overstriking pipe original outer diameter；The open slot for being opened in 31 side of fixing seat and being connected with its inner cavity 33, the width of open slot can be suitable with intracavity diameter；It is separately positioned on a pair of of relative inner wall of the open slot 33 of fixing seat 31 On a pair of of card slot 34 (card slot on the inner sidewall of side is only shown in Fig. 3 a)；It is placed in open slot 33 and its both ends passes through Connecting shaft and a pair of of card slot 34 are slidably connected the idler wheel 35 moved so as to the length extending direction along fixing seat 31.

Wherein, card slot 34 extends along the length direction of fixing seat 31, but development length is less than the length of fixing seat, and card slot 34 extended line can intersect at a point with the central axis of 31 inner cavity of fixing seat, so that idler wheel 35 is in Manual-pushing and along open slot 33 When mobile, the caliber position that the outer rim of idler wheel 35 protruded into open slot squeezes overstriking pipe 2 changes, to make overstriking pipe 2 The volume in the channel being connected to capillary 1 changes, and then capillary 1 is made to draw or be discharged liquid.

When manufacture, the fixing seat 31 of rectangular taper type (or wedge type) as shown in Figure 3a is can be used in fixing seat 31, can also To use the fixing seat in truncated cone as shown in Figure 4 (side external surface of the frustum of a cone is plane).In order in pushing rolling wheels The liquid that the stroke for rolling idler wheel 35 just makes capillary 1 draw or be discharged meets setting value, and the utility model exists The both ends of fixing seat 31 are separately provided for limiting maximum the first limiting card 32 for rolling stroke of idler wheel 35 and the second limit Card 36, the first limiting card 32 and the second limiting card 36 can be located at the both ends of open slot 33, and protrude from fixing seat outwardly Outer surface.It preferably, can also be in some position between the first limiting card 32 and the second limiting card 36 of fixing seat 31 The capacity of the liquid that capillary 1 is discharged when the place's of setting setting is for prompting idler wheel 35 to roll at the position is just 10 microlitres and mentions Show button 37 (as shown in Figure 3b), it, can be in the convex of the upper surface setting protrusion positioned at 33 side of open slot of fixing seat 31 when manufacture Block can also print graduation mark in upper surface or mark is used as cue button as cue button.It the position of cue button 37 can basis It calculates and determines.

For liquid capacity in clear mark capillary, the utility model on the outer wall of capillary along capillary it is axial according to The secondary mark scale for being provided with 5 μ l, 10 μ l, 15 μ l and 20 μ l allows operator accurately to observe capillary and draws and arrange Amount of liquid out.It and is convenient for pushing rolling wheels, in also settable screw thread or the rolling for being used to increase frictional force of the peripheral surface of idler wheel Knife mark.

Wherein, the tubular structure for becoming driving assembly 3 of the overstriking of the present embodiment can borrow the use used in prior art infusion In the flow regulator for adjusting transfusion speed, but flow regulator is compared, the first limiting card 32, second limit is set in fixing seat Position card 36, or even also set up cue button 37.It should be noted that, although the tubular structure for becoming driving assembly 3 of overstriking and flow velocity tune It is similar to save device, but its role in this application is different from the effect of the flow regulator of the prior art, structure also has It is different.

Alternatively, the tubular change driving assembly 3 of the overstriking of the utility model can also use structure as shown in Figure 5, comprising: tool There is the fixing seat 31a of the inner cavity for being set with overstriking pipe 2；The opening for being opened in the side fixing seat 31a and being connected with its inner cavity Slot 33a；Card slot 34a on an inner sidewall of the open slot 33a of fixing seat 31 is set；Be placed in open slot 33a and its One end is slidably connected the idler wheel 35a moved so as to the length extending direction along fixing seat 31a by connecting shaft and card slot 34a, The sliding block of the surface sliding for being located at another inner sidewall place open slot 33a side along fixing seat 31a is arranged in the other end.With above-mentioned figure The structure of 3a is identical, and the card slot 34a of this structure extends along the length direction of fixing seat 31a, and the extended line of card slot 34a and fixation The central axis of the inner cavity seat 31a intersects at a point, and is respectively arranged with the first limiting card 32a and second at the both ends of fixing seat 31a Limiting card 36a.Equally, cue button can also be set between the first limiting card 32a and the second limiting card 36a.

Fixing seat 31a uses taper type shown in fig. 5, and the side of taper type have relatively fixed seat inner cavity it is symmetrical in Heart identity distance is from two different planes, that is, the relatively fixed seat inner cavity of the plane positioned at the two sides open slot 33a of fixing seat 31a It is differed along the distance between symmetrical centre face that fixing seat length direction extends, one where relatively large plane 37a Card slot 34a is set on the inner sidewall of the open slot of side, the connecting shaft (not shown) connecting with the one end idler wheel 35a center is pacified It sets in card slot 34a, so that the connecting shaft can move in card slot 34a when operator pushing rolling wheels 35a.And with idler wheel 35a The sliding block of other end center connection is placed on relatively small plane 38a, and can be along the planar slide.For the ease of pushing away Dynamic idler wheel 35a simultaneously deviate from idler wheel 35a will not out of open slot, and the present embodiment is installed one end on idler wheel 35a and is overlapped in admittedly Passage frame 39a on the relatively small plane 38a of the distance of reservation, passage frame 39a are in rectangle frame shape, dew of the frame in idler wheel 35a On part outside for open slot, elapses frame a part and the sliding block of the idler wheel 35a other end is connected as one, another part holding On the inner sidewall of the open slot apart from relatively large plane 37a side, correspondingly, apart from relatively large plane In addition to being arranged other than the card slot for being clamped connecting shaft on the inner sidewall of the open slot of 37a side, it is additionally provided with passage frame card Slot, when to push passage frame, a part for elapsing frame is slided along apart from relatively small plane 38a, elapses another portion of frame Point moved along passage frame card slot apart from the side relatively large plane 37a, when design, passage frame card slot with apart from relatively small Plane 38a elevation it is corresponding.

In actual use, the quantitative micro inoculator of the utility model can be packed using independent, aseptic package bag, and It is disposable, to avoid the cross contamination in use process between different liquids sample.

In the following, description the utility model is quantitatively micro to be connect for using the tubular change driving assembly 3 of overstriking shown in Fig. 3 The use process of kind device.

The tubular change driving assembly of assembled overstriking is sleeved on outside overstriking pipe, it is when suit, the size of fixing seat is larger End is placed in below overstriking pipe, size small end is placed in (as shown in Figure 1) above.

Before use, the tubular idler wheel for becoming driving assembly of overstriking to be placed on to first limit of open slot one end (i.e. lower section) Ka Chu, idler wheel squeeze overstriking pipe outer wall, so that the tubular change of overstriking is minimum, (i.e. overstriking tube cavity channel is maximum, and internal diameter is first at this time The internal diameter of beginning state), capillary is then inserted perpendicularly into sample liquid level to be tested hereinafter, to the open slot other end is located at Shifting roller at second limiting card of (i.e. top), in the process, deformation occurs is gradually increased for overstriking pipe, i.e. overstriking tube cavity Channel gradually becomes smaller, so that the air pressure in the channel that overstriking pipe is connected to capillary becomes larger, so that the portion inside overstriking pipe be discharged Divide air.Then it is moved down at idler wheel to the first limiting card along open slot, so that the tubular change of overstriking is gradually reduced, i.e. overstriking pipe Bore passages become larger, and air pressure becomes smaller in the channel that overstriking pipe is connected to capillary, the air pressure in external atmosphere pressure and channel Under the action of fine difference, liquid is drawn in capillary by capillary, when liquid is drawn to 20 μ l graduation mark of capillary, by capillary Pipe is separated with liquid to stop drawing liquid, inoculator is transferred to culture medium, then move up at idler wheel to cue button, passed through Squeezing overstriking pipe makes at capillary discharge sample (liquid drawn) to 10 μ l graduation marks, so that 10 μ l of accurate inoculation can be realized Sample is to culture medium.

Using the quantitative micro inoculator seed sample of the utility model, other methods with the prior art are (such as with 10 μ l Pipettor, the import oese etc. of adjustable range) seed sample compares, have at low cost, no cross contamination, accuracy it is high, Easy advantage hand-manipulated, and can realize large-scale production, it is especially suitable for vast basic hospital laboratory and routinely uses, it can Make up the deficiency of existing operating method.

Although being explained in detail above to the utility model, the utility model is without being limited thereto, the skill of the art Art personnel can be modified with principle according to the present utility model, therefore, all according to the various of the principles of the present invention progress Modification all should be understood as falling into the protection scope of the utility model.

Claims (8)

1. a kind of quantitative micro inoculator of Micro biological Tests characterized by comprising

Its front end is for contacting the capillary to be tested with liquid；

The overstriking pipe for being set to capillary tail end and being connected to the lumen of capillary；

Being mounted on overstriking pipe is used to make overstriking pipe to generate deformation so that the liquid that capillary draws liquid or discharge has been drawn The tubular change driving assembly of overstriking；

Wherein, the outer diameter and inner diameter of the overstriking pipe is respectively greater than the outer diameter and inner diameter of the capillary；

Wherein, the outer wall of the capillary is provided with the scale for identifying liquid capacity in it, and the capillary is transparent Plastic tube, the overstriking pipe be the transparent plastic flexible pipe that can produce deformation.

2. quantitative micro inoculator according to claim 1, which is characterized in that the tubular change driving assembly packet of overstriking It includes:

Fixing seat with the inner cavity for being set with the overstriking pipe；

The open slot for being opened in fixing seat side and being connected with its inner cavity；

A pair of of the card slot being separately positioned on a pair of of relative inner wall of fixing seat open slot；

It is placed in open slot and its both ends is slidably connected to extend along the length of fixing seat by connecting shaft and a pair of of card slot The mobile idler wheel in direction；

Wherein, the card slot extends along the length direction of the fixing seat；

Wherein, the both ends of the fixing seat are separately provided for limiting the first limiting card and the second limit of the rolling stroke of idler wheel Position card.

3. quantitative micro inoculator according to claim 2, which is characterized in that first limiting card and second limit Position card is located at the both ends of the open slot.

4. quantitative micro inoculator according to claim 2 or 3, which is characterized in that the extended line of the card slot with it is described The central axis of fixing seat inner cavity intersects at a point.

5. quantitative micro inoculator according to claim 1, which is characterized in that the tubular change driving assembly packet of overstriking It includes:

Fixing seat with the inner cavity for being set with the overstriking pipe；

The open slot for being opened in fixing seat side and being connected with its inner cavity；

Card slot on one inner sidewall of fixing seat open slot is set；

It is placed in open slot and one end is slidably connected by connecting shaft and card slot so as to along the length extending direction of fixing seat The sliding block of the surface sliding for being located at another inner sidewall place of open slot side along fixing seat is arranged in mobile idler wheel, the other end；

Wherein, the card slot extends along the length direction of the fixing seat；

Wherein, the both ends of the fixing seat are separately provided for limiting the first limiting card and the second limit of the rolling stroke of idler wheel Position card.

6. quantitative micro inoculator according to claim 5, which is characterized in that the extended line of the card slot and the fixation The central axis of seat inner cavity intersects at a point.

7. quantitative micro inoculator according to claim 6, which is characterized in that the tubular change driving assembly of overstriking also wraps It includes and is mounted on the idler wheel and one end is overlapped in passage frame on the surface of the fixing seat.

8. quantitative micro inoculator according to claim 1, which is characterized in that the internal diameter of the capillary is 0.5mm- 1.5mm, the internal diameter of the overstriking pipe are 8mm-12mm.