CN209493551U - A kind of DNA synthesis column - Google Patents
A kind of DNA synthesis column Download PDFInfo
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- CN209493551U CN209493551U CN201820110443.XU CN201820110443U CN209493551U CN 209493551 U CN209493551 U CN 209493551U CN 201820110443 U CN201820110443 U CN 201820110443U CN 209493551 U CN209493551 U CN 209493551U
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- solid phase
- liquid storage
- phase carrier
- storage chamber
- column
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Abstract
A kind of DNA synthesis column, solid phase carrier including column tube, in column tube, the solid phase carrier is divided into the first solid phase carrier and second solid phase carrier setting up and down, the inner cavity of the column tube is followed successively by entrance, the first liquid storage chamber, the first lock chamber, the second liquid storage chamber, the second lock chamber, liquid outlet from top to bottom, first solid phase carrier is stuck in the first lock chamber, the second solid phase support card is in the second lock chamber, the uniformly narrow contracting from top to bottom respectively of the internal diameter of first liquid storage chamber and the second liquid storage chamber.The utility model provides a kind of DNA synthesis column with two pieces of solid phase carriers, is applicable to use in the DNA technique of two synthesis, while passing through the setting of the first liquid storage chamber and the second liquid storage chamber, stablizes flow rate of liquid；Simultaneously synthesizing rod structure is reasonable, easy to assembly, and stability is good.
Description
Technical field
The utility model belongs to biological medicine experiment consumptive material field, and in particular to a kind of DNA synthesis column.
Background technique
With the progress of knowledge and the development of science and technology, molecular biotechnology development is getting faster, wherein medicament research and development, something lost
It passes and is required in the fields such as disease and infectious diseases diagnosis, gene studies, polymerase chain reaction (PCR) and cross experiment largely
DNA fragmentation, these DNA fragmentations require greatly artificial synthesized；The segment of DNA synthesis usually has different expression methods, such as
Oligonucleotides, PCR primer, connector, probe, short strand primer and long strand primer etc..
In laboratory, DNA synthesis is generally synthesized the equipment tools such as column and is completed by DNA synthesizer, DNA.Wherein, DNA is closed
Cheng Zhu is made of column tube and the solid phase carrier being located therein, and solid phase carrier is generally the bead (Controlled for controlling aperture
Pore Glass, abbreviation CPG), pore size can need to select according to experiment.
The existing solid phase carrier for DNA synthesis is divided into two kinds: the first is to load filter membrane up and down, in filter membrane
Between load onto CPG powder.Second is using supra polymer sintering technology that CPG is powder sintered in filter membrane.The first technology
Major defect be it is cumbersome, it is non-to often rely on manpower to complete, and column volume becomes larger after having loaded onto upper and lower filter membrane, if you need to
Obtaining higher condensation rate needs a large amount of excessive column volume reagent to be reacted, and being unfavorable for industrialized production reduces work effect
Rate and the raw material monomer reagent for largely wasting valuableness.Second of sintering CPG synthesis film improves the first CPG synthesis column
The volume of son is unfavorable for greatly the shortcomings that operation, and column volume reduces half compared to the first.For synthesis technology, second
Kind synthesis film reduces half than the first column volume, but column volume is still very big, has both wasted expensive raw material monomer
Reagent is again less than preferable product quality.
In addition, in DNA in the prior art synthesis column, column tube is the pipe fitting of internal diameter gradually narrow contracting, solid in the inner cavity of column tube
Surely there is solid phase carrier, usually a solid phase carrier, or the solid phase carrier for the multiple and different specifications being closely arranged, but thing
In reality, the synthesis column of DNA required for different DNA synthesis technologies also has a difference slightly, in some compound experiments, needs two pieces
Different size of solid phase carrier, at the same needed between two pieces of solid phase carriers spacing for buffer with homogeneous reaction liquid, for this
Problem, the utility model are further studied and improve to DNA in the prior art synthesis column.
Utility model content
For the above deficiency in the prior art, the utility model provides a kind of DNA synthesis column, structurally reasonable, wherein
Solid phase carrier fixation, and there are liquid storage spaces between two pieces solid phase carrier.
In order to solve the above-mentioned technical problem, the utility model is addressed by following technical proposals.
A kind of DNA synthesis column, the solid phase carrier including column tube, in column tube, the solid phase carrier is divided into setting up and down
The first solid phase carrier and second solid phase carrier, the inner cavity of the column tube is followed successively by entrance, the first liquid storage chamber, first from top to bottom
Lock chamber, the second liquid storage chamber, the second lock chamber, liquid outlet, first solid phase carrier are stuck in the first lock chamber, and described second
Solid phase carrier is stuck in the second lock chamber, and the internal diameter of first liquid storage chamber and the second liquid storage chamber is uniformly narrow from top to bottom respectively
Contracting.
Simple DNA in compared with the prior art synthesizes column, provides a kind of two pieces of solid phases of setting in the utility model
The DNA of carrier synthesizes column, while the second liquid storage chamber is equipped between this two pieces of solid phase carriers, carries for temporarily storing from the first solid phase
The liquid left in body is gradually being flowed through from second solid phase carrier after stable and uniform；Second liquid storage chamber has certain height
With liquid storage capacity, when the liquid minimal amount in the second liquid storage chamber, liquid gravity pressure is small, flows down slow, when in the second liquid storage chamber
When amount of liquid is big, liquid gravity pressure is big, and dirty speed is made to become faster, therefore has substantially no effect on whole flow rate of liquid.
Preferably, first solid phase carrier and the second solid phase carrier are cylindrical-shaped structure；Described first is solid
Determining chamber has the cylindrical lumens cooperated with the first solid phase carrier, and second lock chamber has and second solid phase carrier combination
Cylindrical lumens；First solid phase carrier and second solid phase carrier are stuck in the first lock chamber and the second lock chamber.
Preferably, the narrow shrinkage of the second liquid storage chamber internal diameter is greater than the narrow shrinkage of the first liquid storage chamber internal diameter, narrow shrinkage
Small capacity is big, and liquid bottom pressure is small, and flow velocity is relatively small；The big capacity of narrow shrinkage is small, and liquid bottom pressure is big, and flow velocity is opposite
Greatly.
Preferably, first solid phase carrier is the cylindrical-shaped structure of diameter 3mm, high 3mm；The second solid phase carrier
For the cylindrical-shaped structure of diameter 1.5mm, high 1mm, it is respectively provided with different aperture and specification, can be replaced.
Preferably, the height of first lock chamber is 3.5mm, the height of second lock chamber is 1.5mm, is all divided
Not Shao Changyu the first solid phase carrier and second solid phase carrier height, make assembly stablize.
Preferably, the top end diameter of first liquid storage chamber is 5.61mm, the bottom diameter of the first liquid storage chamber is 3mm,
The high 23mm of first liquid storage chamber；The top end diameter of second liquid storage chamber is 3mm, and the bottom diameter of the second liquid storage chamber is 1.5mm, the
The high 7.5mm of two liquid storage chambers.
Preferably, the upper end outer wall of the column tube is equipped with the first circular step and the second circular step, for clamping
Or it is fixed.
Preferably, the bottom end of the column tube is the outlet end that narrow contracting is formed, second lock chamber and liquid outlet are placed in
In the inner cavity of the outlet end.
Compared with prior art, the utility model has the following beneficial effects: providing a kind of with two pieces of solid phase carriers
DNA synthesize column, be applicable to two synthesis DNA technique in use, while pass through the first liquid storage chamber and the second liquid storage chamber
Setting, make flow rate of liquid stablize；Simultaneously synthesizing rod structure is reasonable, easy to assembly, and stability is good.
In addition, the utility model is the shortcomings that having evaded existing synthetic vectors, it is provided simultaneously with following characteristics: one
Pillar can satisfy the synthesis requirement of 0.1nmol-500nmol, and two rigging positions for synthesizing post design subtract to the full extent
Small column volume, can cooperate the synthesis column of industrialized production specification on the market.High-adaptability is also the utility model simultaneously
Feature can adapt to all synthetic instrument equipment uses using synthesis column on the market.
The utility model can be applicable in the oligonucleotide synthesis that a variety of different synthesis require on flow control, being capable of basis
Adjustment aperture size carries out accuracy controlling to flow velocity, and all having not found in presently commercially available all solid phase carriers has flow velocity tune
Control function.Meanwhile accurately control synthetic agent volume is to reach the central characteristics that optimum synthesis condition is the utility model, in the past
The reagent volume of many multiples is needed to can be only achieved the reaction condition of requirement since column volume is larger, however in the mistake of chemical reaction
Cheng Zhong, the synthetic agent of too excessive can cause the broken words of irreversible chemical bond to synthetic DNA chain, as depurination is existing
The pair of the irreversible breaking DNA chain structure as caused by a series of, excess due to reagent such as polybase base phenomenon, peroxidating phenomenon is anti-
It answers.The utility model can design suitable reaction reagent volume, the reaction item for being optimal according to the initial concentration of carrier
Part, on the one hand will not excessively destroy DNA chain structure, on the other hand will not measure and reaction is caused not exclusively to greatly improve and synthesize less
Rate and the condensation rate for improving the single base of DNA obtain the higher high-purity DNA chain structure of quality to reduce side reaction.
Detailed description of the invention
Fig. 1 is the schematic cross-section of the column tube in the utility model.
Fig. 2 is the perspective view of the first solid phase carrier in the utility model.
Fig. 3 is the perspective view of the second solid phase carrier in the utility model.
Fig. 4 is the schematic cross-section of the first solid phase carrier in the utility model.
Fig. 5 is the schematic cross-section of the second solid phase carrier in the utility model.
Fig. 6 is the schematic cross-section of the DNA synthesis column in the utility model.
Fig. 7 is the schematic cross-section of the first solid phase carrier of another embodiment in the utility model.
Fig. 8 is the schematic cross-section of the second solid phase carrier of another embodiment in the utility model.
Specific embodiment
The utility model is described in further detail with specific embodiment with reference to the accompanying drawing.
Referring to figs. 1 to Fig. 6, a kind of DNA involved in the utility model synthesizes column, and column tube 3 including tubulose is set to column tube
Solid phase carrier in 3, the solid phase carrier are divided into the first solid phase carrier 1 and second solid phase carrier 2 setting up and down, the column tube
3 inner cavity be followed successively by from top to bottom entrance 31, the first liquid storage chamber 33, the first lock chamber, the second liquid storage chamber 34, the second lock chamber,
Liquid outlet 32, first solid phase carrier 1 are stuck in the first lock chamber, and the second solid phase carrier 2 is stuck in the second lock chamber,
The uniformly narrow contracting from top to bottom respectively of the internal diameter of first liquid storage chamber 33 and the second liquid storage chamber 34.
In present embodiment, first solid phase carrier 1 and the second solid phase carrier 2 are cylindrical-shaped structure；It is described
First lock chamber has the cylindrical lumens cooperated with the first solid phase carrier 1, and second lock chamber has to be carried with second solid phase
The cylindrical lumens that body 2 cooperates, the first solid phase carrier 1 and second solid phase carrier 2 are stuck in the first lock chamber and second and fix
In chamber.The narrow shrinkage of second liquid storage chamber, 34 internal diameter is greater than the narrow shrinkage of 33 internal diameter of the first liquid storage chamber, for controlling and adjusting
Flow velocity.The upper end outer wall of the column tube 3 is equipped with the first circular step 35 and the second circular step 36, is used for gripping apparatus grips
Bottom end Deng, the column tube 3 is the outlet end 37 that narrow contracting is formed, and second lock chamber and liquid outlet 32 are placed in the outlet end 37
Inner cavity in；The outer diameter of outlet end 37 is 2.75mm, is facilitated insertion into container.
When specific to certain size, in present embodiment, first solid phase carrier 1 is the circle of diameter 3mm, high 3mm
Column structure；The second solid phase carrier 2 is the cylindrical-shaped structure of diameter 1.5mm, high 1mm.The height of first lock chamber
For 3.5mm, the height of second lock chamber is 1.5mm.The top end diameter of first liquid storage chamber 33 is 5.61mm, the first storage
The bottom diameter of sap cavity 33 is 3mm, the high 23mm of the first liquid storage chamber 33；The top end diameter of second liquid storage chamber 34 be 3mm, second
The bottom diameter of liquid storage chamber 34 is 1.5mm, the high 7.5mm of the second liquid storage chamber 34.The DNA that the structure is suitable for two synthesis technologies is closed
At middle use.
The first solid phase carrier 1 and second solid phase that Fig. 7 and Fig. 8 is shown in the utility model in another embodiment carry
Body 2, difference are circumferentially to be respectively equipped with the first flexible seal ring 11 up and down in the first solid phase carrier 1, carry in second solid phase
Body 2 is circumferentially respectively equipped with the second flexible seal ring 21 up and down, and the first flexible seal ring 11 and the second flexible seal ring 21 can
Thinking elastic rubber material or other deformable flexible materials, guaranteeing when for assembling close between solid phase carrier and lock chamber
Envelope, while improving Assembly stability.
DNA in the utility model synthesizes column, equipped with the two pieces of solid phase carriers designed, while this two pieces of solid phase carriers it
Between be equipped with the second liquid storage chamber 34, for temporarily storing the liquid left from the first solid phase carrier, after stable and uniform gradually from
It is flowed through in second solid phase carrier 2；Second liquid storage chamber 34 has certain height and liquid storage capacity, the liquid in the second liquid storage chamber
When minimal amount, liquid gravity pressure is small, flows down slow, when amount of liquid is big in the second liquid storage chamber 34, liquid gravity pressure is big, under making
The speed of stream becomes faster, therefore has substantially no effect on whole flow rate of liquid.
Specifically, the DNA in the utility model synthesizes column, pass through the interval of the first solid phase carrier 1 and second solid phase carrier 2
Setting, cooperates the solid phase carrier of different size, can be controlled in -10ml/ minutes 0.05ml/ minutes ranges with flow rate of liquid；This
In utility model, synthesize membrane aperture within the scope of 0.1nm-100nm, synthetic vectors specification within the scope of 0.1nmol-500nmol,
The optional different size of synthetic vectors volume size, wherein the first solid phase carrier, within the scope of 0.05ul-1.5ul, second solid phase carries
Body is within the scope of 0.7ul-25ul；Synthesis base range is 1bp-300bp, and mutation rate range 0.1 ‰ -0.001 ‰ is being tested
It is applied widely in research process.
The above, the DNA in the utility model synthesize column, are applicable to use in the DNA technique of two synthesis, together
When by the setting of the first liquid storage chamber 33 and the second liquid storage chamber 34, stablize flow rate of liquid；Simultaneously synthesizing rod structure is reasonable, assembly
Convenient, stability is good.
The protection scope of the utility model includes but is not limited to embodiment of above, and the protection scope of the utility model is to weigh
Subject to sharp claim, replacement, deformation, the improvement that those skilled in the art that any pair of this technology is made is readily apparent that are each fallen within
The protection scope of the utility model.
Claims (8)
1. a kind of DNA synthesizes column, including column tube (3), the solid phase carrier being set in column tube (3), which is characterized in that the solid phase carries
Body is divided into the first solid phase carrier (1) and second solid phase carrier (2) setting up and down, the inner cavity of the column tube (3) from top to bottom according to
It is secondary be entrance (31), the first liquid storage chamber (33), the first lock chamber, the second liquid storage chamber (34), the second lock chamber, liquid outlet (32),
First solid phase carrier (1) is stuck in the first lock chamber, and the second solid phase carrier (2) is stuck in the second lock chamber, described
The uniformly narrow contracting from top to bottom respectively of the internal diameter of first liquid storage chamber (33) and the second liquid storage chamber (34).
2. a kind of DNA according to claim 1 synthesizes column, which is characterized in that first solid phase carrier (1) and described the
Two solid phase carriers (2) are cylindrical-shaped structure；First lock chamber is cylindric interior with cooperating with the first solid phase carrier (1)
Chamber, second lock chamber have the cylindrical lumens cooperated with second solid phase carrier (2).
3. a kind of DNA according to claim 1 synthesizes column, which is characterized in that the second liquid storage chamber (34) internal diameter it is narrow
Shrinkage is greater than the narrow shrinkage of the first liquid storage chamber (33) internal diameter.
4. a kind of DNA according to any one of claims 1 to 3 synthesizes column, which is characterized in that first solid phase carrier
It (1) is diameter 3mm, the cylindrical-shaped structure of high 3mm；The second solid phase carrier (2) is the cylindric knot of diameter 1.5mm, high 1mm
Structure.
5. a kind of DNA according to claim 4 synthesizes column, which is characterized in that the height of first lock chamber is
3.5mm, the height of second lock chamber are 1.5mm.
6. a kind of DNA according to claim 5 synthesizes column, which is characterized in that the top of first liquid storage chamber (33) is straight
Diameter is 5.61mm, and the bottom diameter of the first liquid storage chamber (33) is 3mm, the first liquid storage chamber (33) high 23mm；Second liquid storage chamber
(34) top end diameter is 3mm, and the bottom diameter of the second liquid storage chamber (34) is 1.5mm, the second liquid storage chamber (34) high 7.5mm.
7. a kind of DNA according to claim 6 synthesizes column, which is characterized in that the upper end outer wall of the column tube (3) is equipped with
First circular step (35) and the second circular step (36).
8. a kind of DNA according to claim 6 synthesizes column, which is characterized in that the bottom end of the column tube (3) is narrow contracting formation
Outlet end (37), second lock chamber and liquid outlet (32) are placed in the inner cavity of the outlet end (37).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820110443.XU CN209493551U (en) | 2018-01-23 | 2018-01-23 | A kind of DNA synthesis column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820110443.XU CN209493551U (en) | 2018-01-23 | 2018-01-23 | A kind of DNA synthesis column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209493551U true CN209493551U (en) | 2019-10-15 |
Family
ID=68150446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820110443.XU Active CN209493551U (en) | 2018-01-23 | 2018-01-23 | A kind of DNA synthesis column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209493551U (en) |
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2018
- 2018-01-23 CN CN201820110443.XU patent/CN209493551U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A DNA synthesis column Effective date of registration: 20220707 Granted publication date: 20191015 Pledgee: Jiangsu Rugao Rural Commercial Bank Co.,Ltd. Jiang'an sub branch Pledgor: Nantong Heruiyuan Biotechnology Co.,Ltd. Registration number: Y2022320010346 |