CN209476307U - A kind of micro-fluidic chip for stem cell excretion body separation and concentration - Google Patents
A kind of micro-fluidic chip for stem cell excretion body separation and concentration Download PDFInfo
- Publication number
- CN209476307U CN209476307U CN201920181739.5U CN201920181739U CN209476307U CN 209476307 U CN209476307 U CN 209476307U CN 201920181739 U CN201920181739 U CN 201920181739U CN 209476307 U CN209476307 U CN 209476307U
- Authority
- CN
- China
- Prior art keywords
- interface channel
- entrance
- feed pathway
- outlet
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000029142 excretion Effects 0.000 title claims abstract description 25
- 238000000926 separation method Methods 0.000 title claims abstract description 16
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 13
- 230000037361 pathway Effects 0.000 claims description 75
- 239000007788 liquid Substances 0.000 claims description 24
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000002159 nanocrystal Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 description 24
- 239000007853 buffer solution Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001808 exosome Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000003312 immunocapture Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008816 organ damage Effects 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 210000004738 parenchymal cell Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
Abstract
The utility model relates to a kind of micro-fluidic chips for stem cell excretion body separation and concentration, belong to medical domain.Micro-fluidic chip described in the utility model includes mixing pit I；The mixing pit I passes sequentially through collecting pit I, interface channel I, mixing pit II, interface channel II and is connected to collecting pit II；And the collecting pit I can have magnetic field or without magnetic field, I two sides of interface channel are respectively equipped with the entrance III of the entrance II of interface channel I, interface channel I on corresponding position, II two sides of interface channel are respectively equipped with the entrance III of the entrance II of interface channel II, interface channel II on corresponding position, and the collecting pit II can have magnetic field or without magnetic field.The utility model has the beneficial effect that the excretion nanocrystal composition of available high-purity.
Description
Technical field
The utility model relates to a kind of micro-fluidic chips for stem cell excretion body separation and concentration, belong to medical domain.
Background technique
Mescenchymal stem cell is a kind of multipotential stem cell, has multinomial differentiation potential, reparation and regeneration to body tissue
It is of great significance.Recently, a large number of studies show that in stem-cell therapy, effect of the transplanted cells in tissue repair, mainly
The activity that damage location surrounding material cell is stimulated by paracrine mechanism, is rather than directly to specific parenchymal cell transformation and replaces
For damaged tissues.Excretion body is the extracellular vesicles (Extracellular vesicles or EV) that most cells can all be secreted,
Diameter is 20-140nm.It contains derived cell nucleic acid abundant and protein, and is widely present among various body fluid.In the recent period
Research also indicates that mescenchymal stem cell excretion body plays the role of the regeneration of a variety of organ damage models, dry thin with mesenchyma
Born of the same parents' role is consistent, and more and more datas highlight potentiality of this kind of excretion body in reparation and regeneration.
The excretion body of source for mesenchymal stem cells can be shifted by the albumen of its content, RNA ingredient intercellular, play and
Mesenchymal stem cells similar tissue repair, adjusts the effects of immune function at immunosupress.And compared with simple cell treatment
Compared with excretion volume property is more stable, saves convenient transportation, forms wind without transplanted cells bring immunological rejection and tumour
Danger.Therefore more and more evidences show that new cell therapy-mescenchymal stem cell secretion excretion body (Exosomes) can be made
For a kind of more noticeable alternative medicine, because advantages possessed by them are greater than corresponding mescenchymal stem cell.
However, current existing excretion body separation method, such as: there are still such as supercentrifugation, immunomagnetic beads method etc.
Consuming time is long, the expensive limitations such as low with separation purity of complicated, equipment complex for operation step, be not possible to fully meet scientific research and
Demand of the clinic to high-purity excretion body.So having become at present in the world to quick, the separation of high-purity excretion body research
The research hotspot of related fields and the task of top priority.
It is micro-fluidic to refer to using microchannel (having a size of a few micrometers to hundreds of microns) processing or manipulation minute fluid (volume
For nanoliter arrive A Sheng) system involved in Science and Technology, be one be related to chemistry, fluid physics, microelectronics, new material,
The emerging cross discipline of biology and biomedical engineering.Microfluidic system fast, separating sample with separation biologic grain speed
Purity is high saves the unique advantages such as sample, integrated level height.Therefore, although application of the microflow control technique in terms of excretion body separation
It is still at an early stage, but have become the hot spot of current research.
Summary of the invention
The utility model, come separation and concentration stem cell excretion body, solves above-mentioned ask by the micro-fluidic chip of new construction
Topic.
The utility model provides a kind of micro-fluidic chip for stem cell excretion body separation and concentration, the micro-fluidic core
Piece includes mixing pit I, collecting pit I, interface channel I, mixing pit II, interface channel II, collecting pit II；The mixing pit I goes out
Mouth is connected to the entrance of collecting pit I；The collecting pit I can have magnetic field or without magnetic field；Described I one end of interface channel, which is equipped with, to be connected
The entrance I in road I is connected, I side of interface channel is equipped with the entrance II of interface channel I, and I other side of interface channel is corresponding
The position of I entrance II of interface channel is equipped with the entrance III of interface channel I, and I other end of interface channel is equipped with interface channel I
Outlet；The outlet of the collecting pit I is connected to the entrance I of interface channel I；The outlet of the interface channel I and mixing pit II
Entrance connection；Described II one end of interface channel is equipped with the entrance I of interface channel II, and II side of interface channel is equipped with connection
The entrance II in channel II, the position that II other side of interface channel is correspondingly connected with II entrance II of channel are equipped with interface channel II
Entrance III, II other end of interface channel be equipped with interface channel II outlet；The outlet of the mixing pit II with connect it is logical
The entrance I in road II is connected to；The outlet of the interface channel II is connected to the entrance of collecting pit II；The collecting pit II can have magnetic
Or without magnetic field.
The utility model is preferably that the mixing pit I is sinuous structure.
The utility model is preferably that the mixing pit II is sinuous structure.
The utility model is preferably that the micro-fluidic chip includes inlet I, feed pathway I, inlet II, feed pathway
Ⅱ；The entrance of the feed pathway I is connected to inlet I, and the outlet of the feed pathway I is connected to the entrance of mixing pit I；Institute
The entrance for stating feed pathway II is connected to inlet II, and the outlet of the feed pathway II is connected to the entrance of mixing pit I；It is described
Feed pathway I and II mirror symmetry of feed pathway.
The utility model is preferably that the micro-fluidic chip includes inlet III, feed pathway III, feed pathway IV；It is described
The entrance of feed pathway III is connected to inlet III, and the outlet of the feed pathway III is connected to the entrance II of interface channel I；Institute
The entrance for stating feed pathway IV is connected to inlet III, and the outlet of the feed pathway IV is connected to the entrance III of interface channel I；
The feed pathway III and IV mirror symmetry of feed pathway.
The utility model is preferably that the micro-fluidic chip includes inlet IV, feed pathway V, feed pathway VI；It is described
The entrance of feed pathway V is connected to inlet IV, and the outlet of the feed pathway V is connected to the entrance II of interface channel II；
The entrance of the feed pathway VI is connected to inlet IV, and the outlet of the feed pathway VI and the entrance III of interface channel II connect
It is logical；The feed pathway V and VI mirror symmetry of feed pathway.
The utility model is preferably that the micro-fluidic chip includes liquid outlet；The outlet of the collecting pit II and liquid outlet connect
It is logical.
The application method of micro-fluidic chip described in the utility model includes the following steps:
1. the anti-X antibody pre-coated with magnetic bead (Mag) of the sample containing excretion body is mixed, it is compound to form Mag-X-Exo
Object；
2. Mag-X-Exo compound is added to inlet I, anti-X antibody (Ab1) is added to inlet II, Mag-X-
Exo compound enters mixing pit I simultaneously with anti-X antibody (Ab1) and mixes in mixing pit I, and it is compound to form Mag-X-Exo-Ab1
Object；
3. first making collecting pit I have magnetic field close to collecting pit I magnet, Mag-X-Exo-Ab1 compound is divided by magnetic field
From being enriched in collecting pit I, then PBS buffer solution is added to inlet II and cleans Mag-X-Exo-Ab1 compound；
4. removing the magnet of collecting pit I, make collecting pit I without magnetic field, fluorescence secondary antibody (Ab2) is added to inlet III, with
Mag-X-Exo-Ab1 compound mixes in mixing pit II, forms Mag-X-Exo-Ab1-Ab2 compound；
5. first making collecting pit II have magnetic field close to collecting pit II magnet, Mag-X-Exo-Ab1-Ab2 is answered by magnetic field
Close object separation and concentration in collecting pit II, then by PBS buffer solution be added to inlet IV clean Mag-X-Exo-Ab1-Ab2 it is compound
Object.
The utility model has the beneficial effect that
1. it is logical that fluorescence secondary antibody (Ab2) is injected into connection by the entrance II of interface channel I, the entrance III of interface channel I simultaneously
In road I, two side impact Mag-X-Exo-Ab1 compounds of fluorescence secondary antibody (Ab2) liquid stream from Mag-X-Exo-Ab1 compound liquid stream
Liquid stream makes fluorescence secondary antibody (Ab2) liquid stream and Mag-X-Exo-Ab1 compound liquid stream form whirlpool in interface channel I, is conducive to
Further in mixing pit II of fluorescence secondary antibody (Ab2) and Mag-X-Exo-Ab1 compound mixes.
2. PBS buffer solution is injected into interface channel by the entrance II of interface channel II, the entrance III of interface channel II simultaneously
In II, PBS buffer solution liquid stream is entered in interface channel II from the two sides of interface channel II and is impacted mutually, makes PBS buffer solution
Liquid stream forms whirlpool in interface channel II, is conducive to PBS buffer solution and cleans the Mag-X-Exo-Ab1-Ab2 in collecting pit II
Compound.
Detailed description of the invention
1 width of the utility model attached drawing,
Fig. 1 is the structural schematic diagram of micro-fluidic chip described in embodiment 1；
Wherein: 1, inlet I, 2, feed pathway I, 3, inlet II, 4, feed pathway II, 5, mixing pit I, 6, collecting pit
I, 7, interface channel I, 8, inlet III, 9, feed pathway III, 10, feed pathway IV, 11, mixing pit II, 12, interface channel
II, 13, inlet IV, 14, feed pathway V, 15, feed pathway VI, 16, collecting pit II, 17, liquid outlet.
Specific embodiment
Following non-limiting embodiments can make those skilled in the art that the utility model be more fully understood, but
The utility model is not limited in any way.
Embodiment 1
A kind of micro-fluidic chip for stem cell excretion body separation and concentration, as shown in Figure 1, the micro-fluidic chip includes
Inlet I 1, inlet II 3, feed pathway II 4, the mixing pit I 5 of sinuous structure, collecting pit I 6, connects feed pathway I 2
Connect road I 7, inlet III 8, feed pathway III 9, feed pathway IV 10, sinuous structure mixing pit II 11, interface channel
II 12, inlet IV 13, feed pathway V 14, feed pathway VI 15, collecting pit II 16, liquid outlet 17；
The entrance of the feed pathway I 2 is connected to inlet I 1, the outlet of the feed pathway I 2 and entering for mixing pit I 5
Mouth connection；
The entrance of the feed pathway II 4 is connected to inlet II 3, the outlet of the feed pathway II 4 and mixing pit I 5
Entrance connection；
And the feed pathway I 2 and II 4 mirror symmetry of feed pathway；
The outlet of the mixing pit I 5 is connected to the entrance of collecting pit I 6；
When magnet is close to collecting pit I 6, the collecting pit I 6 has magnetic field；
When removing the magnet of the collecting pit I 6, the collecting pit I 6 is without magnetic field；
Described I 7 one end of interface channel is equipped with the entrance I of interface channel I 7, and it is logical that I 7 side of interface channel is equipped with connection
The entrance II in road I 7, the position that I 7 other side of interface channel is correspondingly connected with I 7 entrance II of channel are equipped with interface channel I 7
Entrance III, I 7 other end of interface channel are equipped with the outlet of interface channel I 7；
The outlet of the collecting pit I 6 is connected to the entrance I of interface channel I 7；
The entrance of the feed pathway III 9 is connected to inlet III 8, the outlet of the feed pathway III 9 and interface channel I
7 entrance II is connected to；
The entrance of the feed pathway IV 10 is connected to inlet III 8, the outlet of the feed pathway IV 10 with connect it is logical
The entrance III in road I 7 is connected to；
And the feed pathway III 9 and IV 10 mirror symmetry of feed pathway；
The outlet of the interface channel I 7 is connected to the entrance of mixing pit II 11；
Described II 12 one end of interface channel is equipped with the entrance I of interface channel II 12, and II 12 side of interface channel is equipped with
The entrance II of interface channel II 12, the position that II 12 other side of interface channel is correspondingly connected with II 12 entrance II of channel are equipped with
The entrance III of interface channel II 12, II 12 other end of interface channel are equipped with the outlet of interface channel II 12；
The outlet of the mixing pit II 11 is connected to the entrance I of interface channel II 12；
The entrance of the feed pathway V 14 is connected to inlet IV 13, the outlet of the feed pathway V 14 with connect it is logical
The entrance II in road II 12 is connected to；
The entrance of the feed pathway VI 15 is connected to inlet IV 13, the outlet of the feed pathway VI 15 with connect it is logical
The entrance III in road II 12 is connected to；
And the feed pathway V 14 and VI 15 mirror symmetry of feed pathway；
The outlet of the interface channel II 12 is connected to the entrance of collecting pit II 16；
When magnet is close to collecting pit II 16, the collecting pit II 16 has magnetic field；
When removing the magnet of the collecting pit II 16, the collecting pit II 16 is without magnetic field；
The outlet of the collecting pit II 16 is connected to liquid outlet 17.
Embodiment 2
A method of it being coated with the excretion body of anti-X antibody capture expression X using magnetic bead, described method includes following steps:
1. the anti-X antibody pre-coated with magnetic bead (Mag) of the blood plasma containing excretion body is mixed, it is compound to form Mag-X-Exo
Object；
2. Mag-X-Exo compound to be injected to the inlet I 1 of micro-fluidic chip described in embodiment 1, by anti-X antibody (Ab1)
Inlet II 3 is injected, Mag-X-Exo compound and anti-X antibody (Ab1) are simultaneously into mixing pit I 5 and abundant in mixing pit I 5
Reaction forms Mag-X-Exo-Ab1 compound；
3. first making collecting pit I 6 have magnetic field close to collecting pit I 6 magnet, by magnetic field by Mag-X-Exo-Ab1 compound
Separation and concentration cleans Mag-X-Exo-Ab1 compound in collecting pit I 6, then by PBS buffer solution injection inlet II 3, to improve
The purity of Mag-X-Exo-Ab1 compound；
4. removing the magnet of collecting pit I 6, make collecting pit I 6 without magnetic field, fluorescence secondary antibody (Ab2) is injected into inlet III 8, it is glimmering
Light secondary antibody (Ab2) is injected into interface channel I 7 by the entrance II of interface channel I 7, the entrance III of interface channel I 7 simultaneously, fluorescence
Secondary antibody (Ab2) liquid stream makes glimmering from two side impact Mag-X-Exo-Ab1 compound liquid streams of Mag-X-Exo-Ab1 compound liquid stream
Light secondary antibody (Ab2) liquid stream and Mag-X-Exo-Ab1 compound liquid stream form whirlpool in interface channel I 7, are conducive to fluorescence secondary antibody
(Ab2) it is sufficiently reacted in mixing pit II 11 with Mag-X-Exo-Ab1 compound, forms Mag-X-Exo-Ab1-Ab2 compound；
5. first making collecting pit II 16 have magnetic field close to collecting pit II 16 magnet, by magnetic field by Mag-X-Exo-Ab1-
Ab2 compound separation and concentration injects inlet IV 13 in collecting pit II 16, then by PBS buffer solution, and PBS buffer solution is simultaneously by even
The entrance III of the entrance II, interface channel II 12 of connecting road II 12 is injected into interface channel II 12, and PBS buffer solution liquid stream is from even
The two sides for connecting road II 12 enter in interface channel II 12 and impact mutually, make PBS buffer solution liquid stream in interface channel II 12
Interior formation whirlpool is conducive to PBS buffer solution and cleans the Mag-X-Exo-Ab1-Ab2 compound in collecting pit II 16, to improve
The purity of Mag-X-Exo-Ab1-Ab2 compound；
6. directly utilizing the expression of X in fluorescence microscope detection excretion body in collecting pit II 16；Or remove collecting pit
II 16 magnet makes collecting pit II 16 without magnetic field, the excretion body of immunocapture is collected by liquid outlet 17, recycles fluorescence microscope
Detect the expression of X in excretion body.
Claims (7)
1. a kind of micro-fluidic chip for stem cell excretion body separation and concentration, it is characterised in that: the micro-fluidic chip includes
Mixing pit I, collecting pit I, interface channel I, mixing pit II, interface channel II, collecting pit II；
The outlet of the mixing pit I is connected to the entrance of collecting pit I；
The collecting pit I can have magnetic field or without magnetic field；
Described I one end of interface channel is equipped with the entrance I of interface channel I, and I side of interface channel is equipped with entering for interface channel I
Mouth II, the position that I other side of interface channel is correspondingly connected with I entrance II of channel is equipped with the entrance III of interface channel I, described
I other end of interface channel is equipped with the outlet of interface channel I；
The outlet of the collecting pit I is connected to the entrance I of interface channel I；
The outlet of the interface channel I is connected to the entrance of mixing pit II；
Described II one end of interface channel is equipped with the entrance I of interface channel II, and II side of interface channel is equipped with interface channel II
Entrance II, II other side of interface channel be correspondingly connected with II entrance II of channel position be equipped with interface channel II entrance
III, II other end of interface channel is equipped with the outlet of interface channel II；
The outlet of the mixing pit II is connected to the entrance I of interface channel II；
The outlet of the interface channel II is connected to the entrance of collecting pit II；
The collecting pit II can have magnetic field or without magnetic field.
2. micro-fluidic chip according to claim 1, it is characterised in that: the mixing pit I is sinuous structure.
3. micro-fluidic chip according to claim 2, it is characterised in that: the mixing pit II is sinuous structure.
4. micro-fluidic chip according to claim 3, it is characterised in that: the micro-fluidic chip includes inlet I, feed liquor
Channel I, inlet II, feed pathway II；
The entrance of the feed pathway I is connected to inlet I, and the outlet of the feed pathway I is connected to the entrance of mixing pit I；
The entrance of the feed pathway II is connected to inlet II, and the outlet of the feed pathway II and the entrance of mixing pit I connect
It is logical；
The feed pathway I and II mirror symmetry of feed pathway.
5. micro-fluidic chip according to claim 4, it is characterised in that: the micro-fluidic chip include inlet III, into
Liquid channel III, feed pathway IV；
The entrance of the feed pathway III is connected to inlet III, the outlet of the feed pathway III and the entrance of interface channel I
II connection；
The entrance of the feed pathway IV is connected to inlet III, the outlet of the feed pathway IV and the entrance of interface channel I
III connection；
The feed pathway III and IV mirror symmetry of feed pathway.
6. micro-fluidic chip according to claim 5, it is characterised in that: the micro-fluidic chip include inlet IV, into
Liquid channel V, feed pathway VI；
The entrance of the feed pathway V is connected to inlet IV, the outlet of the feed pathway V and the entrance of interface channel II
II connection；
The entrance of the feed pathway VI is connected to inlet IV, the outlet of the feed pathway VI and the entrance of interface channel II
III connection；
The feed pathway V and VI mirror symmetry of feed pathway.
7. micro-fluidic chip according to claim 6, it is characterised in that: the micro-fluidic chip includes liquid outlet；
The outlet of the collecting pit II is connected to liquid outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920181739.5U CN209476307U (en) | 2019-01-31 | 2019-01-31 | A kind of micro-fluidic chip for stem cell excretion body separation and concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920181739.5U CN209476307U (en) | 2019-01-31 | 2019-01-31 | A kind of micro-fluidic chip for stem cell excretion body separation and concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209476307U true CN209476307U (en) | 2019-10-11 |
Family
ID=68133557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920181739.5U Expired - Fee Related CN209476307U (en) | 2019-01-31 | 2019-01-31 | A kind of micro-fluidic chip for stem cell excretion body separation and concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209476307U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021175158A1 (en) * | 2020-03-05 | 2021-09-10 | 清华大学 | Microfluidic channel, microfluidic chip, and method for preparing vesicles |
-
2019
- 2019-01-31 CN CN201920181739.5U patent/CN209476307U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021175158A1 (en) * | 2020-03-05 | 2021-09-10 | 清华大学 | Microfluidic channel, microfluidic chip, and method for preparing vesicles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1324100C (en) | Multiple stage affinity process for isolation of specific cells from a cell mixture | |
| JP6405304B2 (en) | Adipose-derived stem cell production system and method | |
| CN103266050B (en) | For micro-fluidic chip of sorting and application thereof | |
| CN105331580B (en) | Enhance mescenchymal stem cell chemotactic ability and chemokine CCL5 expression | |
| KR101636316B1 (en) | Microfluidic apparatus for isolation, method for isolation using the same, and isolation kit for circulating rare cells using the same | |
| WO2011069117A1 (en) | Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation) | |
| CN108004206A (en) | It is a kind of from the preparation method of people's olfactory mucosa mescenchymal stem cell excretion body and the application of excretion body | |
| CN101638637A (en) | Kit for processing human marrow, cord blood and peripheral blood cells and cell processing method | |
| CN209476307U (en) | A kind of micro-fluidic chip for stem cell excretion body separation and concentration | |
| CN106754344B (en) | The combined sorting purification devices and sorting detection method of circulating tumour cell | |
| Lee et al. | Label-free mesenchymal stem cell enrichment from bone marrow samples by inertial microfluidics | |
| CN113564045A (en) | Micro-fluidic chip and application thereof in tumor stem cell sorting and enrichment | |
| CN110317784A (en) | A kind of preparation method of umbilical cord mesenchymal stem cells excretion body and stem cell beauty, it is anti-ageing, repair and disease treatment in application | |
| CN111220800A (en) | Exosome detection chip based on micro-fluidic droplet technology | |
| CN205687938U (en) | A kind of separation device for adipose-derived stem cells | |
| CN105505858A (en) | Separation and purification method for very small embryonic-like stem cells (VSELs) of pigs | |
| CN101469321A (en) | Method for rapidly filtering and screening medulla ossium substrate stem cell | |
| CN105441386A (en) | Culture and identification method for very small porcine embryonic-like stem cells | |
| CN210193892U (en) | Micro-fluidic chip | |
| CN110004062B (en) | Device and method for sorting and enriching rare circulating tumor cells | |
| CN111893086B (en) | Fetal nucleated red blood cell capture carrier, extraction device and method | |
| CN108977404A (en) | Sizing screening method based on immunomagnetic beads label cell | |
| CN206570309U (en) | A kind of circulating tumor cell sorts enriching apparatus | |
| CN108220233A (en) | Cell separation set surface treatment method, related utensil, peripheral blood rare cell or circulating tumor cell rapidly and efficiently separation method | |
| CN209048767U (en) | A kind of stem cell in vitro enrichment isolation system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191011 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |