CN209412222U - A kind of thermal cycle fluorescence detector - Google Patents
A kind of thermal cycle fluorescence detector Download PDFInfo
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- CN209412222U CN209412222U CN201821974473.1U CN201821974473U CN209412222U CN 209412222 U CN209412222 U CN 209412222U CN 201821974473 U CN201821974473 U CN 201821974473U CN 209412222 U CN209412222 U CN 209412222U
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Abstract
The utility model relates to technical field of fluorescence detection, disclose a kind of thermal cycle fluorescence detector.Including reaction chamber and fluorescence detecting system, reaction chamber is for placing the PCR reaction tube for filling DNA fragmentation to be detected, fluorescence detecting system is used to acquire the fluorescence that liquid is released in PCR reaction tube, fluorescence signal is converted into electric signal output, fluorescence detecting system includes illuminating source, the first interferometric filter, the second interferometric filter, detector lens and face array photoelectric sensor, and the second interferometric filter is set between detector lens and face array photoelectric sensor.First interferometric filter is set between illuminating source and PCR pipe to be detected.The utility model can shorten light path compared with prior art, simplify fluorescence detecting system structure, small in size, light-weight, easy to carry and use.Fluorescence detecting system has high throughput, can be completed at the same time the PCR reaction and fluorescence detection of 96 or 384 or more DNA samples, be not necessarily to optical fiber light-guiding, periodic maintenance is removed in movement-less part abrasion from.
Description
Technical field
The utility model relates to technical field of fluorescence detection, more particularly to a kind of design of thermal cycle fluorescence detector.
Background technique
Thermal cycler is the instrument for making reactant automatic cycle between specified denaturation temperature, renaturation temperature and elongating temperature
Device.When the PCR reaction tube for filling PCR reaction solution and DNA sample is put into thermal cycler, after temperature cycles, sample DNA segment
Million times of amplification.If can be obtained by fluorescence signal in reaction solution added with fluorescein such as SYBR or relevant fluorescent DNA probe
The quantity of DNA fragmentation, by the variation of fluorescence signal in monitoring reaction tube come real-time detection pcr amplification product.Based on face battle array light
The thermal cycler of the fluorescence detecting system of electric transducer imaging has high throughput, can once simultaneously using the QPCR reagent of profession
Complete the PCR reaction and fluorescence detection of 96 or 384 or more DNA samples.But volume is big, it is difficult to carry, basic reason is
Optical length, structure is complicated for fluorescence detecting system, as the light path of Roche LC480 thermal cycle fluorescence detector reaches 800mm.
The fluorescence that PCR reaction tube emits when detection reaches face array photoelectric sensor surface after interferometric filter filters,
The filter effect of interferometric filter is very sensitive to the incident angle of light, and vertical incidence is best, and the angle of off-normal increases,
Central wavelength can be caused mobile to shortwave direction, bandwidth increases, and transmissivity is reduced.If therefore shortening light path merely, will increase
The incidence angle of light, influences testing result.Publication No. CN1309766A's enters the state of China on November 15th, 2000
Border patent discloses the instrument for monitoring of DNA polymerase chain reaction, including a light source, and it is glimmering that transmitting issues basic dyes
The basic excitation frequency of light;The first component, for receiving the light beam of light source as excitation beam with stimulating frequency;Substantially
Focusing block, for focusing on excitation beam in each suspension, so that basic dyes issue the transmitting with tranmitting frequency
Light beam, the basic focusing block can receive and through transmitting light beams;Second component, for receiving from the basic focusing block
Emit light beam;Transmitting focusing component is used to focus emission light beam;Detector receives and comes from second component and transmitting focusing component
Transmitting light beam, generate the elementary data signal for representing the DNA concentration in corresponding each bottle;Processing component utilizes reception
To elementary data signal calculate baseband signal data and corresponding DNA concentration.The first component also includes exciter filter, the
Two components also include launching filter, and the first and second components share an optical splitter, and exciter filter is placed on light source and divides
Between light device, launching filter is placed between optical splitter and detector, and exciter filter makes the light with stimulating frequency logical
It crosses and blocks the light with tranmitting frequency, launching filter then allows the light with tranmitting frequency by blocking with stimulating frequency
Light, exciter filter and optical splitter receive light beam of light source jointly and produce excitation beam, and launching filter and optical splitter are total
Emit light beam so that the transmitting light beam with tranmitting frequency penetrates and reaches detector with receiving.It is needed in the patent using light splitting
Device is divided, while in order to meet the incident corner condition of optical filter, optical splitter need to be placed at an angle, and preferably 45 °,
Instrument internal optical length at this time, the space that optical splitter occupies is big, to cause equipment instrument big, carries and uses inconvenient ask
Topic.
Utility model content
The utility model is directed to the long disadvantage of fluorescence light path in the prior art, provides a kind of thermal cycle fluorescence detector.
The utility model solves the problem of fluorescence light path is long, and shortening optical path will affect interferometric filter effect, the design
Scheme is by changing interferometric filter position, using the optical imaging lens formed by several lens combinations, reaches and is shortening light
Road does not influence the effect of interferometric filter effect simultaneously.
In order to solve the above-mentioned technical problem, the utility model is addressed by following technical proposals:
A kind of thermal cycle fluorescence detector, including reaction chamber and fluorescence detecting system, reaction chamber fill to be checked for placement
The reaction tube of the DNA fragmentation of survey, fluorescence detecting system are used to acquire the fluorescence that liquid is released in reaction tube, fluorescence signal are converted
It is exported at electric signal, fluorescence detecting system includes illuminating source, the first interferometric filter, detector lens, the second interferometric filter
And face array photoelectric sensor, the second interferometric filter are set between detector lens and face array photoelectric sensor;Detector lens include
Object lens and optical imaging lens, object lens be set between reaction tube and optical imaging lens, the second interferometric filter be set to optics at
As between camera lens and face array photoelectric sensor.Object lens can be one group, or multiple groups, multiple groups object lens either simultaneously or alternately work
Make.Optical imaging lens can be one group, or multiple groups, multiple groups optical imaging lens either simultaneously or alternately work.Face battle array light
Electric transducer can be one group, or multiple groups, multiple groups face array photoelectric sensor either simultaneously or alternately work.
When detection, the fluorescence detecting system of thermal cycler needs the light that by excitation light path, light source is issued to pass through first
Interferometric filter filters, and obtains the exciting light of specific wavelength, and be oriented to the PCR reaction tube for needing exciting light to irradiate.Exposure
Group launches specific fluorescence, and the fluorescence of transmitting reaches face array photoelectric sensor surface after collecting.Before arrival, need
To pass through the fluorescence of the second interferometric filter leaching specific wavelength.The thermal cycle fluorescence that tradition is imaged based on face array photoelectric sensor
Detector is that the second interferometric filter is placed on before optical imaging lens, between PCR reaction tube and optical imaging lens, face
Array photoelectric sensor is placed on behind optical imaging lens.The second interferometric filter is placed in detector lens and face battle array light in the application
Between electric transducer, detector lens bring together the fluorescence for entering camera lens, and fluorescence is almost to filter perpendicular through the second interference
Mating plate.Control incident angle of the light by interferometric filter is had to using interferometric filter filtering fluorescence, makes incident angle
Less than 25 °, more preferably less than 12 °.The incidence angle of this practical middle fluorescence is almost 0 °, and can satisfy interferometric filter uses item
Part reaches preferable filter effect.The biggish wide-angle lens of field angle can be used at this time, while shortening object lens and optical imaging lens
The distance between head, achievees the purpose that not influence testing result while shortening light path.
Preferably, further including electronic controller, electronic controller controls reaction chamber and fluorescence detecting system work, receives
The detected value of fluorescence detecting system, outside output test result.Electronic controller will test value and be sent to information processing system.Letter
Breath processing system can be a part of instrument, also may not be that can be localization, is also possible to long-range host computer.
Preferably, optical imaging lens are formed by several lens combinations, field angle is 2 ° -180 ° after combination.Lens it
Between orderly form a line by straight line.
Preferably, optical imaging lens are the wide-angle optical lens of 60 ° of -180 ° of field angles.Using the optical imaging lens
Head, the light path from object lens to face array photoelectric sensor surface can achieve only 100mm or be less than 100mm.Tradition is based on relatively
The thermal cycle fluorescence detector of face array photoelectric sensor imaging, the application can effectively shorten light path.
Preferably, optical imaging lens are formed by four sheet glass lens and two plastic lens compositions.
Preferably, the first interferometric filter is set between illuminating source and the reaction tube for filling DNA fragmentation to be detected.
Preferably, the second interferometric filter and optical imaging lens assemble to form one, or with face battle array photoelectric sensing
Device assembles to form one.
Preferably, being equipped with dichronic mirror or reflective mirror between object lens and optical imaging lens.
Preferably, reaction chamber includes temperature cycles unit, temperature cycles unit includes semiconductor cooler;Or including
Refrigerator and heater, refrigerator are fan or water cooling plant, and heater is heating wire or electromagnetic wave heating device.
Preferably, illuminating source is light emitting diode, Organic Light Emitting Diode, halogen lamp, xenon lamp, one in laser
Kind or several combinations.
The utility model has significant technical effect: can shorten light path, simplify due to using above technical scheme
Fluorescence detecting system structure, it is small in size, it is light-weight, it is easy to carry and use.Fluorescence detecting system can simultaneously amount detection it is more
Sample, or even can be completed at the same time 96 or 384 or more DNA samples PCR reaction and fluorescence detection, it is widely applicable, can
To meet the use demand of a variety of occasions.Without optical fiber light-guiding, periodic maintenance is removed in movement-less part abrasion from.
Detailed description of the invention
Fig. 1 is prior-art illustration.
Fig. 2 is the structural schematic diagram of the utility model embodiment 1.
Fig. 3 is the optical imaging lens structural schematic diagram of the utility model embodiment 1.
Fig. 4 is the structural schematic diagram of the utility model embodiment 2.
Fig. 5 is the optical imaging lens structural schematic diagram of the utility model embodiment 2.
Fig. 6 is the optical imaging lens structural schematic diagram of the utility model embodiment 3.
Fig. 7 is the structural schematic diagram of the utility model embodiment 11.
Fig. 8 is the structural schematic diagram of the utility model embodiment 12.
Fig. 9 is the structural schematic diagram of the utility model embodiment 23.
The toponym that each number designation is referred in the figures above is as follows: where 1-illuminating source, the 2-the first interference
Optical filter, 3-detector lens, the 4-the second interferometric filter, 5-face array photoelectric sensors, 8-reaction tubes, 9-reaction chambers,
31-object lens, 32-optical imaging lens.
Specific embodiment
The utility model is described in further detail with embodiment with reference to the accompanying drawing.
Embodiment 1
As shown in Figure 1, in the prior art, the patent of invention of Publication No. CN1309766A discloses poly- for monitoring of DNA
The instrument of polymerase chain reaction.The instrument includes a light source, and transmitting makes the basic excitation frequency of basic dyes sending fluorescence;
The first component, for receiving the light beam of light source as excitation beam with stimulating frequency;Basic focusing block, for making to swash
Luminous beam focuses in each suspension, so that basic dyes issue the transmitting light beam with tranmitting frequency, the basic focus portion
Part can receive and through transmitting light beam;Second component, for receiving the transmitting light beam from the basic focusing block;Transmitting focusing
Component is used to focus emission light beam;Detector receives the transmitting light beam from second component and transmitting focusing component, generates generation
The elementary data signal of the table DNA concentration in each bottle accordingly;Processing component utilizes the elementary data signal meter received
Calculate baseband signal data and corresponding DNA concentration.
The first component also includes exciter filter, and second component also includes launching filter, and the first and second components are shared
One optical splitter, exciter filter are placed between light source and optical splitter, and launching filter is placed on optical splitter and detector
Between, exciter filter allows the light with stimulating frequency by blocking the light with tranmitting frequency, and launching filter then allows tool
Have the light of tranmitting frequency by blocking the light with stimulating frequency, exciter filter and optical splitter receive light beam of light source jointly and
Excitation beam is produced, launching filter receives transmitting light beam so that the transmitting light beam with tranmitting frequency is saturating with optical splitter jointly
It crosses and reaches detector.
A kind of thermal cycle fluorescence detector disclosed by the utility model, as shown in Figures 2 and 3, including reaction chamber 9 and fluorescence
Detection system, reaction chamber 9 is for placing the reaction tube 8 for filling DNA fragmentation to be detected, and fluorescence detecting system is for acquiring reaction
Fluorescence signal is converted into electric signal output by the fluorescence that liquid is released in pipe 8, and fluorescence detecting system includes illuminating source 1, first
Interferometric filter 2, the second interferometric filter 4, detector lens 3 and face array photoelectric sensor 5, the second interferometric filter 4 are set to inspection
It surveys between camera lens 3 and face array photoelectric sensor 5;Detector lens 3 include object lens 31 and optical imaging lens 32, and object lens 31 are set to anti-
Should be between pipe 8 and optical imaging lens 32, the second interferometric filter 4 is set to optical imaging lens 32 and face array photoelectric sensor 5
Between.
It further include electronic controller, electronic controller controls reaction chamber 9 and fluorescence detecting system work, adjusts reaction chamber 9
Temperature, receive the detected value of fluorescence detecting system, outside output test result.
Optical imaging lens 32 are formed by several lens combinations.Optical imaging lens 32 are moulded by four sheet glass lens and two
Material lens combination is formed, and four sheet glass lens and two plastic lens orderly form a line by straight line, and optical imaging lens 32 are burnt
Away from for 2.8mm, field angle is 110 °.
Reaction chamber 9 includes temperature cycles unit, and temperature cycles unit is metal alloy material specimen holder, specimen holder upper surface
Tapered blind hole is dug to insert PCR reaction tube 8, specimen holder bottom laminated semiconductor refrigerator.Electronic controller is by adjusting half
The size of current of conductor refrigerator and direction adjust the temperature of specimen holder, to adjust the temperature of reaction tube 8.Illuminating source 1
For light emitting diode, face array photoelectric sensor is Sony Corporation's cmos imaging chip I MX274.
In the present embodiment, 32 chief ray angle of optical imaging lens is less than 15 °, apart from 5 surface of face array photoelectric sensor
3.6mm can place the second interferometric filter 4 of thickness 3.6mm particular range of wavelengths below, 32 overall length of optical imaging lens
18mm.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens is 234mm, at this time from object lens
The light path on 31 to 5 surface of face array photoelectric sensor only has 100mm light path.Tradition is imaged based on face array photoelectric sensor 5 relatively
The fluorescence detecting system of the 800mm light path of thermal cycle fluorescence detector, the short light path of 100mm of the invention has structure simple, body
The small outstanding advantages of product.
Embodiment 2
A kind of thermal cycle fluorescence detector, as shown in Figure 4 and Figure 5, detector lens 3 include object lens 31 and light in the present embodiment
Imaging lens 32 are learned, optical imaging lens 32 are formed by 5 lens combinations, and field angle is 60 °.Using the optical imaging lens 32
Thermal cycle fluorescence detector, it is assumed that 31 diameter of object lens be 234mm, at this time from object lens 31 to 5 surface of face array photoelectric sensor
Light path only has 220mm.Light source 1 is set to 3 two sides of detector lens in the present embodiment.Temperature cycles unit includes refrigerator and heating
Device, refrigerator are fan, and heater is heating wire.Illuminating source 1 is the combination of Organic Light Emitting Diode, halogen lamp and xenon lamp.
Remaining is all same as Example 1.
Embodiment 3
A kind of thermal cycle fluorescence detector, as shown in fig. 6, in the present embodiment detector lens 3 include object lens 31 and optics at
As camera lens 32, optical imaging lens 32 are formed by 7 lens combinations, and field angle is 70 °.Using the heat of the optical imaging lens 32
Recycle fluorescence detector, it is assumed that 31 diameter of object lens is 234mm, at this time from object lens 31 to the light path on 5 surface of face array photoelectric sensor
Only 185mm.Temperature cycling element includes refrigerator and heater in the present embodiment, and refrigerator is water cooling plant, and heater is
Electromagnetic wave heating device.Illuminating source 1 is laser.Remaining is all same as Example 1.
Embodiment 4
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 80 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 158mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining is all same as Example 1.
Embodiment 5
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 90 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 135mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining is all same as Example 1.
Embodiment 6
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 100 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that object lens 31 are straight
Diameter is 234mm, there was only 116mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 7
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 120 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that object lens 31 are straight
Diameter is 234mm, there was only 86mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 8
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 140 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that object lens 31 are straight
Diameter is 234mm, there was only 61mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 9
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 160 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that object lens 31 are straight
Diameter is 234mm, there was only 39mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 10
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 180 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that object lens 31 are straight
Diameter is 234mm, there was only 19mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 11
As shown in fig. 7, a kind of thermal cycle fluorescence detector, in the present embodiment, the second interferometric filter 4 and optical imaging lens
First 32 assembling forms one, remaining is all same as Example 1.
Embodiment 12
As shown in figure 8, a kind of thermal cycle fluorescence detector, in the present embodiment, the second interferometric filter 4 and face battle array photoelectric transfer
The assembling of sensor 5 forms one, remaining is all same as Example 1.
Embodiment 13
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 50 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 269mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining is all same as Example 1.
Embodiment 14
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 40 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 339.5mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 15
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 30 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 455mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining is all same as Example 1.
Embodiment 16
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 20 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 681.5mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining all with 1 phase of embodiment
Together.
Embodiment 17
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 17 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, it is assumed that 31 diameter of object lens
For 234mm, there was only 801mm from object lens 31 to the light path on 5 surface of face array photoelectric sensor at this time.Remaining is all same as Example 1.
Embodiment 18
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 15 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, object lens in the prior art
31 diameter minimum can reach 33mm.Assuming that 31 diameter of object lens is 33mm, at this time from object lens 31 to 5 surface of face array photoelectric sensor
Light path there was only 143mm.Remaining is all same as Example 1.
Embodiment 19
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 10 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, object lens in the prior art
31 diameter minimum can reach 33mm.Assuming that 31 diameter of object lens is 33mm, at this time from object lens 31 to 5 surface of face array photoelectric sensor
Light path there was only 207mm.Remaining is all same as Example 1.
Embodiment 20
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 5 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, object lens in the prior art
31 diameter minimum can reach 33mm.Assuming that 31 diameter of object lens is 33mm, at this time from object lens 31 to 5 surface of face array photoelectric sensor
Light path there was only 396mm.Remaining is all same as Example 1.
Embodiment 21
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 2.5 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, object in the prior art
The diameter minimum of mirror 31 can reach 33mm.Assuming that 31 diameter of object lens is 33mm, at this time from object lens 31 to 5 table of face array photoelectric sensor
The light path in face only has 774mm.Remaining is all same as Example 1.
Embodiment 22
A kind of thermal cycle fluorescence detector, detector lens 3 include object lens 31 and optical imaging lens 32, light in the present embodiment
Learning 32 field angle of imaging lens is 2 °.Using the thermal cycle fluorescence detector of the optical imaging lens 32, object lens in the prior art
31 diameter minimum can reach 33mm.Assuming that 31 diameter of object lens is 33mm, at this time from object lens 31 to 5 surface of face array photoelectric sensor
Light path there was only 963mm.Remaining is all same as Example 1.
Embodiment 23
A kind of thermal cycle fluorescence detector is equipped with reflective mirror in the present embodiment between object lens 31 and optical imaging lens 32.
Remaining is all same as Example 1.
In short, the above is only the preferred embodiment of the present invention, it is all according to present utility model application the scope of the patents institute
The equivalent changes and modifications of work should all belong to the covering scope of the utility model patent.
Claims (10)
1. a kind of thermal cycle fluorescence detector, including reaction chamber (9) and fluorescence detecting system, reaction chamber (9) is filled for placing
The reaction tube (8) of DNA fragmentation to be detected, fluorescence detecting system are used to acquire the fluorescence that liquid is released in reaction tube (8), will be glimmering
Optical signal is converted into electric signal output, it is characterised in that: fluorescence detecting system includes illuminating source (1), the first interferometric filter
(2), detector lens (3), the second interferometric filter (4) and face array photoelectric sensor (5), the second interferometric filter (4) are set to inspection
It surveys between camera lens (3) and face array photoelectric sensor (5);Detector lens (3) include object lens (31) and optical imaging lens (32), object
Mirror (31) is set between reaction tube (8) and optical imaging lens (32), and the second interferometric filter (4) is set to optical imaging lens
(32) between face array photoelectric sensor (5).
2. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: it further include electronic controller, electricity
Sub-controller controls reaction chamber (9) and fluorescence detecting system work, receives the detected value of fluorescence detecting system, outward output detection
As a result.
3. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: optical imaging lens (32) if by
Dry lens combines to be formed, and field angle is 2 ° -180 ° after combination.
4. a kind of thermal cycle fluorescence detector according to claim 3, it is characterised in that: optical imaging lens (32) are
The wide-angle optical lens of 60 ° of -180 ° of field angles.
5. a kind of thermal cycle fluorescence detector according to claim 3, it is characterised in that: optical imaging lens (32) are by four
Sheet glass lens and two plastic lens compositions are formed.
6. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: the first interferometric filter (2) is set
Between illuminating source (1) and the reaction tube (8) for filling DNA fragmentation to be detected.
7. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: the second interferometric filter (4) with
Optical imaging lens (32) assembling forms one, or assembles with face array photoelectric sensor (5) and form one.
8. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: object lens (31) and optical imaging lens
Dichronic mirror or reflective mirror are equipped between head (32).
9. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: reaction chamber (9) includes that temperature is followed
Ring element, temperature cycles unit include semiconductor cooler, or including refrigerator and heater;Refrigerator is fan or water cooling
Device, heater are heating wire or electromagnetic wave heating device.
10. a kind of thermal cycle fluorescence detector according to claim 1, it is characterised in that: illuminating source (1) is luminous two
The combination of one or more of pole pipe, Organic Light Emitting Diode, halogen lamp, xenon lamp, laser.
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