CN209311402U - Aflatoxin detection device in a kind of liquid - Google Patents
Aflatoxin detection device in a kind of liquid Download PDFInfo
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- CN209311402U CN209311402U CN201821916527.9U CN201821916527U CN209311402U CN 209311402 U CN209311402 U CN 209311402U CN 201821916527 U CN201821916527 U CN 201821916527U CN 209311402 U CN209311402 U CN 209311402U
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- raman
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- sampling valve
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Abstract
The utility model relates to aflatoxin detection devices in a kind of liquid, including feed liquor bottle, infusion pump, sampling valve, column oven, temperature control plate, refrigeration backing, fan, laser, glass box, SERS substrate, Raman probe, Raman spectrometer, waste liquid bottle, STM32 single-chip microcontroller, host computer and liquid crystal display.Infusion pump work pressurization after instrument powers on, AFT sample to be measured is added by sampling valve, and enter in the C18 chromatographic column in column oven with mobile phase, each AFT is separated under affinity chromatography effect, the SERS substrate in glass box is sequentially entered, the raman spectral signal that Raman probe test sample generates utilizes the vibration frequency of various AFT molecules and the corresponding relationship of its Raman scattering property, determine the type and concentration of AFT, last result is transferred to liquid crystal display by serial ports and host computer is shown.Liquid-phase chromatographic column is combined with SERS detection technique, there is the features such as sample is high without preparation, selectivity and sensitivity, and analysis speed is fast.
Description
Technical field
The utility model relates to technical field of optical detection, it particularly relates to which aflatoxin detects in a kind of liquid
Device.
Background technique
Aflatoxin AFT (Aflatoxin) is toxic by one kind of Mycophyta such as Aspergillus flavus, aspergillus parasiticus bacterium generation
Secondary metabolite, mainly include this 6 class of AFB1, AFB2, AFG1, AFG2, AFM1, AFM2.Aflatoxin is defended by the world
It is 1 class carcinogenic substance that raw tissue, which delimited, and toxicity is 68 times bigger than arsenic, is only second to meat poisoning mycin, is to be currently known mycetism most
Strong.It is reported that the harmfulness of aflatoxin is to be woven with destruction to people and animal's liver group, liver cancer can lead to when serious
It is even dead.Not only toxicity is big for aflatoxin, content is low, and pollution range is wide, in grain, oil, tea, dry fruit, cream, feed, sauce
Discovery has aflatoxin contamination in more than the 100 kinds of agricultural product such as oil and food.Therefore, carry out Determination Technology of Aft to grind
Study carefully particularly significant.
It is many about the detection method of aflatoxin at present, mainly there are thin layer chromatography (TLC), fluorimetry, height
Effect liquid phase chromatogram method (HPLC) and high performance liquid chromatography-tandem mass method (HPLC-MS/MS).TLC method is interfered vulnerable to impurity,
Sensitivity, precision and reproduction degree are bad.Fluorimetry has high sensitivity, the good, amount of samples of selectivity few and operation letter
Just the advantages that.A kind of Huang of the patent disclosure for having patent disclosure 206862897U of aspergillus flavus detection is carried out currently with this technology
Aspertoxin detection device.But the fluorescence of burst of ultraviolel is very weak, and interferes vulnerable to other fluorescent materials, leads to intensity image
Resolution ratio is lower and noise is poor, will affect the accuracy of detection.HPLC method has good analytical effect, high sensitivity, divides
Analyse that speed is fast, good selective, HPLC method is most widely used in the detection of aflatoxin.Currently with this
Technology carries out the aflatoxin liquid chromatographic detection of the patent disclosure for having patent publication No. 205484221U of aspergillus flavus detection
Device.But HPLC method is usually using fluorescence detector (FLD), and carbon-carbon double bond electrophilic lures in two furan structure of B1 and G1 molecule
Effect is led, molecular fluorescence remitted its fury is caused, influences method sensitivity.Although fluorescence intensity can be enhanced by deriving, but still
There are deriveding analysis time length, step is more, interference is more, the deficiency of false positive easily occurs.HPLC-MS/MS method is that a kind of collection is high
Effect separation and multicomponent are qualitative, quantitative in integrated joint technology, have destructiveness to sample, carry out purification pretreatment etc.,
Detection time is long, is not suitable for the industrial on-line checking of high-volume.And sample is analyzed using Raman spectrum detection technique, more
The shortcoming of the above detection technique is mended.Raman spectrum detection technique is in addition to, non-destructive untouchable with test sample
Outside, also have the characteristics that detection rapidly, result is accurate, needs that sample size is few, sample is without preparing.
Summary of the invention
For the above-mentioned problems in the prior art, the utility model proposes aflatoxin detection dresses in a kind of liquid
It sets, its object is to optimizing detection steps, shorten detection time, improve detection efficiency, while keeping testing result more accurate.
In order to solve the above problem, the utility model adopts the following technical solution:
Aflatoxin detection device in a kind of liquid, by separation system (1), thermostatic control system (2), SERS detection system
It unites (3), waste liquid bottle (4), STM32 single-chip microcontroller (5), host computer (6) and liquid crystal display (7) form；Separation system (1) is located at constant temperature
On the left of control system (2), SERS detection system (3) is located on the right side of thermostatic control system (2), and waste liquid bottle (4) is located at SERS detection
The lower section of system (3), STM32 single-chip microcontroller (5) are located at the left side of thermostatic control system (2) and the lower section of separation system (1), liquid crystal
Screen (7) is fixed on STM32 single-chip microcontroller (5) by I/O port, and host computer (6) is located at below STM32 single-chip microcontroller (5), and the two passes through
Data line is connected；Wherein separation system (1) includes feed liquor bottle (101), infusion pump (102) and sampling valve (103), the feed liquor bottle
(101) it is located at the left side of sampling valve (103), and is connected to sampling valve (103) by infusion pump (102)；Thermostatic control system (2)
Including column oven (201), temperature control plate (202) and refrigeration backing and fan (203), the column oven (201) is a column
Closed aluminium alloy box, C18 chromatographic column, temperature control plate (202) and refrigeration backing are placed in inside and fan (203) is located at column oven
(201) lower section；SERS detection system (3) includes laser (301), glass box (302), SERS substrate (303), Raman probe
(304) and Raman spectrometer (305)；The laser (301) is located at the left side of glass box (302), and SERS substrate (303) is attached
The silicon wafer of nano-Ag particles, be located in glass box (302), the optical path of excitation light source is in 90 ° and vertical with the optical path of Raman signal
The detection mouth of Raman probe (304) is injected, Raman probe (304) is connected to Raman spectrometer (305).
The utility model has the following beneficial effects:
Aflatoxin detection device uses separation system in a kind of liquid of the utility model, can accurately separate
Different types of aflatoxins (such as: AFB1, AFB2, AFG1, AFG2 etc.)；
It controls C18 chromatographic column by chromatogram column temperature control at 25 DEG C (± 1 DEG C) using thermostatic control system and AFT is divided
From effect, the accuracy of testing result is promoted；
Using the vibration frequency of various aflatoxin molecules and the corresponding relationship of its Raman scattering property, greatly improve
The accuracy of detection efficiency and testing result；
Using the STM32F103RCT6 single-chip microcontroller of ST company, its maximum functional clock is 72MHz, possesses 256K
FLASH, 48K SRAM, 5 serial ports, 8 16 bit timing devices, SPI communication function.It can receive and send instructions under host computer, on
Conduction pump pressure, the data such as column oven temperature；Receive control instruction, the synchronizing information of sampling valve of host computer, control pump, laser light
The Push And Release in source sets the flow of pump, the temperature of column oven.
Detailed description of the invention
With reference to the accompanying drawing and embodiment is described further the utility model.
Fig. 1 is the structural schematic diagram of aflatoxin detection device in a kind of liquid of the utility model.
Fig. 2 is the structural schematic diagram of the SERS detection system of the utility model.
It is thermostatic control system, (3) be SERS detection system, (4) is waste liquid bottle, (5) that (1), which is separation system, (2), in figure
It is STM32 single-chip microcontroller, (6) be host computer, (7) be liquid crystal display, (101) be feed liquor bottle, (102) be infusion pump, (103) is sample introduction
Valve, (201) are that column oven, (202) are that temperature control plate, (203) are refrigeration backings and fan, (301) be laser, (302) are glass
It is Raman probe, (305) is Raman spectrometer that box, (303), which are SERS substrate, (304),.
Specific embodiment
As shown in Figure 1, 2, aflatoxin detection device in a kind of liquid, by separation system (1), thermostatic control system
(2), SERS detection system (3), waste liquid bottle (4), STM32 single-chip microcontroller (5), host computer (6) and liquid crystal display (7) composition；Separation
System (1) is located on the left of thermostatic control system (2), and SERS detection system (3) is located on the right side of thermostatic control system (2), waste liquid bottle
(4) it is located at the lower section of SERS detection system (3), STM32 single-chip microcontroller (5) is located at left side and the segregative line of thermostatic control system (2)
The lower section of system (1), liquid crystal display (7) are fixed on STM32 single-chip microcontroller (5) by I/O port, and host computer (6) is located at STM32 single-chip microcontroller
(5) lower section, the two are connected by data line；Wherein separation system (1) includes feed liquor bottle (101), infusion pump (102) and sampling valve
(103), the feed liquor bottle (101) is located at the left side of sampling valve (103), and is connected to sampling valve by infusion pump (102)
(103)；Thermostatic control system (2) includes column oven (201), temperature control plate (202) and refrigeration backing and fan (203), described
Column oven (201) is a closed aluminium alloy box of column, and C18 chromatographic column, temperature control plate (202) and refrigeration backing are placed in inside
It is located at the lower section of column oven (201) with fan (203)；SERS detection system (3) include laser (301), glass box (302),
SERS substrate (303), Raman probe (304) and Raman spectrometer (305)；The laser (301) is located at glass box (302)
Left side, SERS substrate (303) are to adhere to the silicon wafer of nano-Ag particles, are located in glass box (302), the optical path and Raman of excitation light source
The optical path of signal is in 90 ° and vertically injects the detection mouth of Raman probe (304), and Raman probe (304) is connected to Raman spectrometer
(305)。
The working principle of aflatoxin detection device in a kind of liquid: sample is added in feed liquor bottle (101), on instrument
It is received after electricity by STM32 single-chip microcontroller (5) and is sent instructions under host computer (6), instruction control infusion pump (102) work is sent by serial ports
Make.Infusion pump (102) work pressurization, AFT sample to be measured are added by sampling valve (103), and period STM32 single-chip microcontroller (5) passes through
The pressure value of serial ports timing inquiry pump, and pressure value is transmitted to host computer (6) and liquid crystal display (7) display by serial ports, and
Single-chip microcontroller (5) receives the synchronization signal of sampling valve (103) by serial ports, controls the work of thermostatic control system (2), controls column temperature
Chromatogram column temperature in case (201) is at 25 DEG C (± 1 DEG C).Single-chip microcontroller (5) inquires the temperature of column oven (201) by serial ports timing
Degree, and temperature is transmitted to host computer (6) and liquid crystal display (7) display by serial ports.Subsequent AFT sample to be measured passes through sampling valve
(103) enter in the C18 chromatographic column in column oven (201) with mobile phase, each AFT is separated under affinity chromatography effect, successively
Into in the SERS substrate (303) in the glass box (302) built in detector.At the same time, single-chip microcontroller (5) is controlled by IO level
Laser (301) emits laser, and surface-enhanced Raman effects occur for laser irradiation sample, and Raman probe (304) detection generates
It Raman signal and is transferred to Raman spectrometer (304), Raman spectrometer (304) is further analyzed raman spectral signal
And processing, analysis determinand is measured using the vibration frequency of aflatoxin molecule and the corresponding relationship of its Raman scattering property
Content is further determined that the type and distribution of aspergillus flavus in sample, will be located by individual features peak in analysis substance Raman spectrum
It manages result and single-chip microcontroller (5) is passed to afterwards by liquid crystal display (7) display by serial ports, sample to be tested is directly discharged into waste liquid bottle after having surveyed
(4)。
Claims (1)
1. aflatoxin detection device in a kind of liquid, by separation system (1), thermostatic control system (2), SERS detection system
(3), waste liquid bottle (4), STM32 single-chip microcontroller (5), host computer (6) and liquid crystal display (7) composition；It is characterized by: separation system
(1) it is located on the left of thermostatic control system (2), SERS detection system (3) is located on the right side of thermostatic control system (2), waste liquid bottle (4) position
In the lower section of SERS detection system (3), STM32 single-chip microcontroller (5) is located at left side and the separation system (1) of thermostatic control system (2)
Lower section, liquid crystal display (7) is fixed on STM32 single-chip microcontroller (5) by I/O port, and host computer (6) is located under STM32 single-chip microcontroller (5)
Side, the two are connected by data line；Wherein separation system (1) includes feed liquor bottle (101), infusion pump (102) and sampling valve
(103), the feed liquor bottle (101) is located at the left side of sampling valve (103), and is connected to sampling valve by infusion pump (102)
(103)；Thermostatic control system (2) includes column oven (201), temperature control plate (202) and refrigeration backing and fan (203), described
Column oven (201) is a closed aluminium alloy box of column, and C18 chromatographic column, temperature control plate (202) and refrigeration heat are placed in inside
Piece and fan (203) are located at the lower section of column oven (201)；SERS detection system (3) include laser (301), glass box (302),
SERS substrate (303), Raman probe (304) and Raman spectrometer (305)；The laser (301) is located at glass box (302)
Left side, SERS substrate (303) are to adhere to the silicon wafer of nano-Ag particles, are located in glass box (302), the optical path and Raman of excitation light source
The optical path of signal is in 90 ° and vertically injects the detection mouth of Raman probe (304), and Raman probe (304) is connected to Raman spectrometer
(305).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821916527.9U CN209311402U (en) | 2018-11-21 | 2018-11-21 | Aflatoxin detection device in a kind of liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821916527.9U CN209311402U (en) | 2018-11-21 | 2018-11-21 | Aflatoxin detection device in a kind of liquid |
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| Publication Number | Publication Date |
|---|---|
| CN209311402U true CN209311402U (en) | 2019-08-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821916527.9U Expired - Fee Related CN209311402U (en) | 2018-11-21 | 2018-11-21 | Aflatoxin detection device in a kind of liquid |
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| CN (1) | CN209311402U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342602A (en) * | 2018-11-21 | 2019-02-15 | 中国计量大学 | Aflatoxin detection device in a kind of liquid |
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2018
- 2018-11-21 CN CN201821916527.9U patent/CN209311402U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342602A (en) * | 2018-11-21 | 2019-02-15 | 中国计量大学 | Aflatoxin detection device in a kind of liquid |
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