CN209193943U - The extraction element of two-phase fermentation liquid under laboratory condition - Google Patents
The extraction element of two-phase fermentation liquid under laboratory condition Download PDFInfo
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- CN209193943U CN209193943U CN201821631282.5U CN201821631282U CN209193943U CN 209193943 U CN209193943 U CN 209193943U CN 201821631282 U CN201821631282 U CN 201821631282U CN 209193943 U CN209193943 U CN 209193943U
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- fermentation liquid
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Abstract
The extraction element of two-phase fermentation liquid under laboratory condition, including temperature hydraulic environment regulation unit, two-phase laminated flow unit, liquid matter extraction unit and cleaning unit.Entire installation for fermenting is located in constant incubator, is equipped with temperature sensor, hydraulic regulating system and constant current hytor in box house.Two-phase laminated flow unit includes producing acid mutually with methanogenic phase device, produces acid mutually in the left side of methanogenic phase, hytor is connect by pumping pressure pipe with methanogenic phase device.It is equipped with miniature aperture at the top of two-phase laminated flow unit, biological selectivity between catheter, extracts fermentation liquid using piston type quantitative extraction tube, cleaning unit is connected with methanogenic phase device through film.Utility model device craftsmanship is strong, easy to operate, low in cost, is fermentation gas, two-phase laminated flow and subsequent metabolic pathway mechanism study is provided convenience and analysis foundation under laboratory condition.
Description
Technical field
The utility model belongs to coal seam bioenergy development field, and in particular to difference produces the reality that gas stage fermentation liquid extracts
Experiment device.
Background technique
Formally effective from 2016 " Paris agreement ", the task of energy-saving and emission-reduction is gradually brought into schedule by countries in the world, I
State has also taken on the emission reduction responsibility of a part.Coal resources are arranged without the unreasonable exploitation of limitation and non-treated burning, discharge
It applies, results in serious environmental pollution and climate change.Utilizing to the exploitation of preservation gas in coal seam is to solve the problems, such as this
Important measures also bring new turn to energy-saving and emission-reduction, environmental protection while reducing ventilation cost and obtaining clean energy resource
Machine.And bury shallowly, the low biogenesis coal bed gas of development cost is the following important channel for realizing gas utilization,
The Basic quota of secondary biogenic gas coal bed gas and exploration and development at present has become one of the hot spot of coal bed gas circle research.Coal
The research and development of layer gas bioengineering are to realization energy-saving and emission-reduction, increase stock number and improve coal seam reservoirs permeability with very important
Meaning.Domestic and foreign scholars have done the formation mechanism of coal biological methane, formation condition, environmental factor and Bacterial community etc.
A large amount of research, but with regard to present analysis status from the point of view of, new drop is not fully clearly also likely to be present to methane constructive ways
Solution produces gas approach, while the variation of product shifts to new management mechanisms that there is also traditional defects among it.In consideration of it, designing newly
Experimental method goes to inquire into new degradation production gas approach that may be present, this is the supplement and bioengineering to traditional zymotic theory
Indispensable one of the step of scale application.
Utility model content
The utility model provides a kind of manufacture craft for the shortcoming shortcoming for the theoretical research that solves to ferment at this stage
Simply, compact-sized, cost is reasonable, experimental performance is strong, can be real on the basis of ensuring the coal anaerobic fermentation environmental optima factor
A kind of experimental determination device of the now separation of phase, the extraction of fermentation liquid and preservation.
To solve the above-mentioned problems, the utility model adopts the following technical solution: two-phase fermentation liquid under laboratory condition
Extraction element, including temperature hydraulic environment regulation unit, two-phase laminated flow unit, liquid matter extraction unit and cleaning unit；Control
Unit includes temperature, hydraulic and environment conditioning, adjusts device using intelligent constant-temperature, is equipped with temperature sensor in box house,
It can consider oneself as when box interior temperature is higher or lower than former set temperature value and carry out cooling or warming temperature, it is constant to reach temperature
Purpose, while box house have thermal insulation layer, be maintained after temperature being made to reach preset value, so that microbial fermentation institute
The temperature factor needed keeps best.Constant current hytor is located at incubator bottom, and two-phase laminated flow device is placed in inside incubator.
Constant current hytor is connect by taking out pressure pipe with methanogenic phase device, and starting voltage indicating device is being pressed at the end of producing sour phase
To produce the liquid matter in sour phase under the action of power can smoothly enter into methanogenic phase.
Two-phase laminated flow device includes producing acid phase and two reaction units of methanogenic phase, and the two reaction is mutually indepedent, mutually not
It influences.It is connected between stream oriented device by corrosion resistance catheter.Catheter interposition is equipped with one layer of biological selectivity through film, and film is adopted
With disposable material and it can extract out and place manually.
Temperature and hydraulic control button are located at outside incubator, are convenient for real-time operation.
It is equipped with miniature aperture (influence for avoiding outside air) at the top of two-phase installation for fermenting, acid-base property can be added manually
Reagent regulates and controls the variation of pH in fermentor.By adjusting the environmental factors such as pH, hydraulic detention time, nutriment element come maximum
The optimum level of the guarantee two-phase yeasting of degree and the separation for indirectly realizing phase.Due to producing micro- life of acid and methanogenic phase
Species group has larger difference to the adaptation range of pH, is producing the variation in sour phase by regulation pH, is obtaining methanogen activity
It is obvious to inhibit, otherwise within the scope of the optimum activity for making pH value be maintained at methanogen in methanogenic phase fermentor, and it is sour to producing
Bacterium generates a degree of inhibiting effect, and then realizes the separation of two-phase relative maximum degree.Divide producing the big of acid phase generation
Son and small-molecule substance enter in catheter under the control of hydraulic device, when penetrating film by biological selectivity, due to big
Molecular substance can not pass through, so that the substance that methanogen is utilized is to be produced acid phase and hydrolyze generated can directly utilize
Small-molecule substance.
Biological selectivity manually adjusts a little through film and miniature aperture, in the collective effect of cooperation hydraulic device, Ji Keshi
The relative separation of two-phase under existing laboratory.It produces acid phase and the separation of methanogenic phase and the extraction of fermentation liquid is not interfere with each other, can synchronize
It carries out.
Fermentation liquid is extracted using piston type quantitative extraction tube, extraction tube bottom end is located at the position in fermentor close to bottom,
Top is connected with hydrops bottle, and extract bottom of the tube position is equipped with Unidirectional water permeable valve, is back to reaction again after preventing lifting liquid
In device.Piston is pulled manually, and the liquid in reaction unit is promoted to tubular body under the effect of the pressure, then depresses work again
Plug, fermentation liquid can be flow in hydrops bottle.Every 3 days extraction one time fermentation liquid.
Hydrops bottle can manually take out, convenient for the timely preservation and processing to fermentation liquid.
After testing, selectivity is transferred through film and uses common domestic water under the drive of hydraulic pressure unit
Achieve the purpose that experimental provision is cleaned and flowed out with leakage fluid dram.
Leakage fluid dram is located at methanogenic phase device bottommost, and is furnished with valve switch.Fermentation liquid and rinse water can be in gravity
Under the action of take advantage of a situation discharge.
Above technical scheme is used, the utility model has the following beneficial effects: the utility model includes four units: temperature
Spend hydraulic environment regulation unit, two-phase laminated flow unit, liquid matter extraction unit and cleaning unit.Control unit includes temperature, liquid
Pressure and environment conditioning adjust device using intelligent constant-temperature, are equipped with temperature sensor when box interior temperature is high in box house
It can consider oneself as when former set temperature value and carry out cooling or warming temperature, to achieve the purpose that temperature is constant, while cabinet
Inside has thermal insulation layer, is maintained after temperature being made to reach preset value, so that temperature factor needed for microbial fermentation is protected
It holds best.Constant current hytor is connect by taking out pressure pipe with methanogenic phase device, is started voltage indicating device at the end of producing sour phase, is being pressed
To produce the liquid matter in sour phase under the action of power can smoothly enter into methanogenic phase.Two-phase laminated flow device includes producing acid phase and methane phase
Two reaction units of phase, and the two reaction is mutually indepedent, is independent of each other.It is connected between stream oriented device by corrosion resistance catheter.It leads
Liquid pipe interposition is equipped with one layer of biological selectivity through film, and film is using disposable material and can transfer and place manually.?
The top of two-phase installation for fermenting is equipped with miniature aperture (influence for avoiding outside air), can add acid-base property reagent regulation hair manually
The variation of pH in fermentation tank.Guaranteed to the greatest extent by adjusting the environmental factors such as pH, hydraulic detention time, nutriment element
The optimum level of two-phase yeasting and the separation for indirectly realizing phase.Due to producing the microbe groups of acid and methanogenic phase to pH
Adaptation range have larger difference, producing in sour phase through the variation of regulation pH, be significantly suppressed methanogen activity, instead
The optimum activity for making pH value be maintained at methanogen in methanogenic phase fermentor within the scope of, and certain journey is generated to acid-producing bacteria
The inhibiting effect of degree, and then realize the separation of two-phase relative maximum degree.Producing the macromolecular and small molecule object that acid phase generates
Matter enters in catheter under the control of hydraulic device, when penetrating film by biological selectivity, since macromolecular substances can not
Pass through, so that the substance that methanogen is utilized is to be produced acid phase to hydrolyze the small-molecule substance generated that can directly utilize.
Fermentation liquid is extracted using piston type quantitative extraction tube, extraction tube bottom end is located at the position in fermentor close to bottom, top and product
Liquid bottle is connected, and extract bottom of the tube position is equipped with Unidirectional water permeable valve, is back in reactor again after preventing lifting liquid.Manually
Piston is pulled, the liquid in reaction unit is promoted to tubular body under the effect of the pressure, then depresses piston, fermentation liquid again
It can flow in hydrops bottle.Every 3 days extraction one time fermentation liquid.Hydrops bottle can manually take out, convenient for the timely guarantor to fermentation liquid
It deposits and handles.Leakage fluid dram is located at methanogenic phase device bottommost, and is furnished with valve switch.After testing, selectivity is transferred
Through film, under the drive of hydraulic pressure unit, using common domestic water be can reach experimental provision cleaning purpose, fermentation liquid and
Rinse water can be flowed out with leakage fluid dram under gravity.
The ingenious difference that optimum PH range is needed according to different fermentations stage Institute of Micro-biology of the utility model, utilizes bioselection
Property through film realize the relative separation that degree is maximized to the liquid matter in different fermentations stage so that two-phase fermentation stage is not done mutually
Disturb, Each performs its own functions, while produce it is sour required small organic molecule mutually can be also provided for methanogenic phase, both improve fermentation efficiency,
The stability of system operation is strengthened again.In addition, further to probe into fermentation, intermediate product is shifted to new management mechanisms in the hope of learning on the way
To the supplement and improvement of traditional zymotic theory, the device can the fermentation liquid of manual withdrawal different phase carry out analysis measurement.It reduces
Experimental cost realizes the initial purpose of design.
In conclusion the utility model manufacture craft is simple, compact-sized, cost is reasonable, experimental performance is strong, it can be true
It protects on the basis of the coal anaerobic fermentation environmental optima factor, realizes the separation of phase, a kind of laboratory survey of broth extraction and preservation
Determine device.Scale application for supplement and bioengineering to traditional zymotic theory provides basic research.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model, and Fig. 2 is the enlarged drawing of hydrops bottle in Fig. 1.
Specific embodiment
As shown in Figure 1, under the laboratory condition of the utility model two-phase fermentation liquid extraction element, including the hydraulic ring of temperature
Border regulates and controls unit, two-phase laminated flow unit, liquid matter extraction unit and cleaning unit；Using Intelligent constant-temperature culture box device 1, in case
Internal portion is equipped with temperature sensor 2, can consider oneself as and cool down when box interior temperature is higher or lower than former set temperature value
Or warming temperature, to achieve the purpose that temperature is constant, while box house has thermal insulation layer 3, after temperature being made to reach preset value
It is maintained, so that temperature factor needed for microbial fermentation keeps best.Constant current hytor 4 is located at incubator bottom 1, and two
Phase-separating device is located inside incubator 1.
Constant current hytor 4 is connect by taking out pressure pipe 5 with methanogenic phase device 6, presses hydraulic button at the end of producing sour phase 7
Switch 8 starts constant current hytor 4, the liquid matter produced in sour phase 7 is made to can smoothly enter into methanogenic phase device 6 under the effect of the pressure
In.
Two-phase laminated flow device includes producing 6 two reaction units of sour phase 7 and methanogenic phase, and the two reaction is mutually indepedent, mutually
It does not influence.It is connected between stream oriented device by corrosion resistance catheter 9.9 interposition of catheter is equipped with one layer of biological selectivity through film
10, film is using disposable material and can extract out and place manually.
Temperature 11 and hydraulic 8 control button are located at outside incubator 1, are convenient for real-time operation.
It is equipped with miniature aperture 12 at the top of two-phase installation for fermenting, pH in acid-base property reagent regulation fermentor can be added manually
Variation.Glass switch 13 is housed, twist switch can add pH adjusting agent, can be to avoid the external world after closing inside miniature aperture
The interference of environment.Guarantee two-phase to the greatest extent by adjusting the environmental factors such as pH, hydraulic detention time, nutriment element
The optimum level of yeasting and the separation for realizing phase indirectly.Microbe groups due to producing acid and methanogenic phase fit pH
It answers range to have larger difference, is producing the variation in sour phase by regulation pH, be significantly suppressed methanogen activity, otherwise
PH value is maintained within the scope of the optimum activity of methanogen in methanogenic phase fermentor, and acid-producing bacteria is generated a degree of
Inhibiting effect, and then realize the maximum relative separation of two-phase.The macromolecular and small-molecule substance that acid phase generates are being produced,
Enter in catheter 9 under the control of constant current hytor 4, by biological selectivity penetrate film 10 when, due to macromolecular substances without
Method passes through, so that the substance that methanogen is utilized is to be produced acid phase to hydrolyze the small molecule object generated that can directly utilize
Matter.
Biological selectivity manually adjusts a little through film 10 and miniature aperture 12, in the collective effect of cooperation constant current hytor 4,
The relative separation of two-phase under laboratory can be realized.It produces sour phase 7 and the separation of methanogenic phase 6 and the extraction of fermentation liquid is not done mutually
It disturbs, progress can be synchronized.
Fermentation liquid is extracted using piston type quantitative extraction tube 14,14 bottom end of extraction tube, which is located in methanogenic phase fermentor 15, to be leaned on
The position of nearly bottom, top are connected with hydrops bottle 16, and 14 bottom position of extract pipe is equipped with Unidirectional water permeable valve 17, prevent liquid
It is back in reactor 15 again after promotion.Piston is pulled manually, and the liquid in reaction unit is promoted to pipe under the effect of the pressure
Internal portion, then depresses piston again, and fermentation liquid can be flow in hydrops bottle 16.Produce the extraction and above-mentioned step of fermentation liquid in sour phase
It is rapid the same.
Hydrops bottle 16 can manually take out, convenient for the timely preservation and processing to fermentation liquid.
After testing, selectivity is transferred through film 10 and uses common domestic water under the drive of hydraulic pressure unit 8
It can reach the purpose of experimental provision cleaning and flowed out with leakage fluid dram 18.
Leakage fluid dram 18 is located at 6 bottommost of methanogenic phase device, and is furnished with valve switch 19.Fermentation liquid and rinse water can be
It takes advantage of a situation under the action of gravity discharge.
Claims (8)
1. the extraction element of two-phase fermentation liquid under laboratory condition, it is characterised in that: regulate and control unit, two including temperature hydraulic environment
Separation units, liquid matter extraction unit and cleaning unit；Using Intelligent constant-temperature culture box device, temperature is installed in box house
Sensor is spent, can consider oneself as when box interior temperature is higher or lower than former set temperature value and carry out cooling or warming temperature, to reach
The purpose constant to temperature increases so that temperature factor needed for microbial fermentation keeps best in the bottom of incubator
One constant current hytor is connect with methanogenic phase device by taking out pressure pipe, starts constant current hytor at the end of producing sour phase, pressing
To produce the liquid matter in sour phase under the action of power can smoothly enter into methanogenic phase, and temperature and hydraulic control button are located at outside incubator
Real-time operation is convenient in portion.
2. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 1, it is characterised in that: box house
With thermal insulation layer, it is maintained after temperature being made to reach preset value.
3. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 2, it is characterised in that: sent out in two-phase
The top of ferment device is equipped with miniature aperture, produces the substance that acid phase generates, and enters methane phase after biological selectivity is through film
In phase device.
4. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 3, it is characterised in that: bioselection
Property through film inside the catheter between two-phase device.
5. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 4, it is characterised in that: use piston
Formula quantifies extraction tube and extracts fermentation liquid, and extraction tube bottom end is located at the position in fermentor close to bottom, and top is connected with hydrops bottle
It connects.
6. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 5, it is characterised in that: hydrops bottle makes
With common triangular flask.
7. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 1, it is characterised in that: experimental provision
Interior remaining fermentation liquid and rinse water can be discharged through leakage fluid dram.
8. the extraction element of two-phase fermentation liquid under laboratory condition according to claim 7, it is characterised in that: leakage fluid dram position
In methanogenic phase device bottommost, and it is furnished with valve switch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821631282.5U CN209193943U (en) | 2018-10-09 | 2018-10-09 | The extraction element of two-phase fermentation liquid under laboratory condition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821631282.5U CN209193943U (en) | 2018-10-09 | 2018-10-09 | The extraction element of two-phase fermentation liquid under laboratory condition |
Publications (1)
| Publication Number | Publication Date |
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| CN209193943U true CN209193943U (en) | 2019-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821631282.5U Expired - Fee Related CN209193943U (en) | 2018-10-09 | 2018-10-09 | The extraction element of two-phase fermentation liquid under laboratory condition |
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| Country | Link |
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| CN (1) | CN209193943U (en) |
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2018
- 2018-10-09 CN CN201821631282.5U patent/CN209193943U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190802 Termination date: 20201009 |