CN209148700U - Kit is used in a kind of detection of lactic dehydrogenase - Google Patents
Kit is used in a kind of detection of lactic dehydrogenase Download PDFInfo
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- CN209148700U CN209148700U CN201821726766.8U CN201821726766U CN209148700U CN 209148700 U CN209148700 U CN 209148700U CN 201821726766 U CN201821726766 U CN 201821726766U CN 209148700 U CN209148700 U CN 209148700U
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- lactic dehydrogenase
- upper cover
- detection
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Abstract
The utility model discloses a kind of lactic dehydrogenase detection kits, including shell, the upper end of the shell is equipped with upper cover, hinge is provided between the shell and upper cover, the side of the upper cover upper end is provided with detection slot, the side of the detection slot is provided with colour atla, the side of the colour atla is provided with protective film, the side of the enclosure interior is provided with reagent placed cavity, side in the reagent placed cavity is placed with oxidation state nicotinamide adenine dinucleotide, the side of the oxidation state nicotinamide adenine dinucleotide is placed with measurement buffer and lactic dehydrogenase enzymatic protective reagent, and measurement buffer is placed on the protectant front end of lactic dehydrogenase, the other side of the enclosure interior is provided with testing cavity, the side of the testing cavity upper end is provided with guide rail, magnetic stripe is provided on rear side of the testing cavity upper end.The utility model, which solves layman, can not carry out rough detection, and additional the problem of preparing container is needed when detection.
Description
Technical field
The utility model relates to reagent detection field, kit is used in specially a kind of lactic dehydrogenase detection.
Background technique
Lactic dehydrogenase is a kind of glycolytic ferment, is the enzyme that can be catalyzed lactic dehydrogenase and generate pyruvic acid, is present in body institute
In the cytoplasm of organized cell, wherein higher with kidney content, in mainly existing in the form of five kinds in animal body, i.e. LDH1,
LDH2, LDH3, LDH4 and LDH5, the lactic dehydrogenase of this five kinds of forms content in different tissues is different, has bright
Aobvious tissue specificity, such as: it is more with LDH1 and LDH2 in the cardiac muscle of the mankind, kidney and red blood cell, in skeletal muscle and liver with
LDH4 and LDH5 are more, and more with LDH3 in the tissue such as lung, spleen, pancreas, thyroid gland, adrenal gland and lymph node, so, it can be by
Initial indication by its tissue specificity as medical diagnosis on disease.Work as mankind or animal according to studies have shown that and receive infection, in vivo
Dehydrogenase Content will rise, such as: when the mankind are with hepatitis, acute hepatocellular injury and when Skeletal muscle injury,
LDH5 will be increased；When with acute viral myocarditis, hemolytic anemia or renal necrosis, LDH1 will be increased.And with dynamic
For object, when cow or sheep only suffer from non-clinical property mazoitis, i.e., when vision or tactile obviously can not discover inflammation symptom, cream
Dehydrogenase Content in the milk of ox or sheep only can rise, and therefore, measure the Dehydrogenase Content in milk, to belong to
A kind of mode of the mazoitis of tentative diagnosis cow or sheep only.
Detection for lactic dehydrogenase is not that the personnel of profession can not carry out detection operation, highly professional characteristic
It is not suitable for self diagnosis or whether illness diagnoses to animal, even is still needed when professional detects additional quasi-
The container of standby detection, it is not convenient enough；Therefore market in urgent need develops a kind of lactic dehydrogenase detection and helps people to solve with kit
Certainly existing problem.
Utility model content
The purpose of this utility model is to provide a kind of lactic dehydrogenase detection kits, to solve above-mentioned background technique
The layman of middle proposition can not carry out rough detection, and additional the problem of preparing container is needed when detection.
To achieve the above object, the utility model provides the following technical solutions: a kind of lactic dehydrogenase detection kit,
Including shell, the upper end of the shell is equipped with upper cover, and hinge is provided between the shell and upper cover, the upper cover upper end
Side is provided with detection slot, and the side of the detection slot is provided with colour atla, and the side of the colour atla is provided with protective film, the shell
The side in internal portion is provided with reagent placed cavity, and the side in the reagent placed cavity is placed with oxidation state nicotinamide adenine two
Nucleotide, the side of the oxidation state nicotinamide adenine dinucleotide are placed with measurement buffer and lactic dehydrogenase enzyme protection
Agent, and measure buffer and be placed on the protectant front end of lactic dehydrogenase, the other side of the enclosure interior is provided with testing cavity,
The side of the testing cavity upper end is provided with guide rail, and magnetic stripe, the upper end of the magnetic stripe are provided on rear side of the testing cavity upper end
It is provided with hinge, the upper end of the hinge is provided with reel, and spring bearing, the reel are provided between the reel and hinge
Two sides be provided with bracket, the middle position inside the upper cover is placed with clamping forceps, and the side inside the upper cover is set
It is equipped with storing chamber, the storing is intracavitary to be placed with reaction medicine film.
Preferably, it the detection slot and puts the height of chamber and is respectively less than the height of upper cover, and the height of detection slot is less than and sets
The height of chamber is put, the size of the protective film is greater than the sectional dimension for putting chamber, and the top of clamping forceps is arranged in the colour atla.
Preferably, sealing joint strip is provided between the upper cover and shell, there are two the hinge settings, and hinge passes through
Fixed screw is connect with upper cover and shell respectively, and the height of the shell is greater than the height of upper cover.
Preferably, there are two the oxidation state nicotinamide adenine dinucleotide settings, the section of the reagent placed cavity
Size is greater than the sectional dimension of testing cavity, is provided with reaction tube in the testing cavity, and reaction tube is provided with 18.
Preferably, the thickness of the hinge is set as half millimeter, and hinge it is adjacent between be provided with connecting line, the magnetic stripe
It is fixedly connected with hinge, the inside of shell is arranged in the bracket, and the length of the reel is greater than the length of hinge, the hinge
Length and magnetic stripe equal length.
Preferably, the bottom end of the clamping forceps is set as arc structure, and the upper end of clamping forceps is set on mouth structure, institute
It states reaction medicine film and is provided with several, be fitted and connected between the reaction medicine film is adjacent.
Compared with prior art, the utility model has the beneficial effects that
1, the utility model reacts medicine film consolidating by lactic dehydrogenase enzyme specificity binding antibody by the setting of reaction medicine film
To determine agent and color developing agent is constituted, the sample drop that layman will test is compareed according to colour developing situation with colour atla in reaction medicine film,
The activity of lactic dehydrogenase in judgement sample that can be rough, and then recognize that the individual of sample whether there is problem, although colour atla
The result precision of comparison is not high enough, but is able to satisfy the needs of detection, and react medicine film be placed on put it is intracavitary, be not contaminated, make
Used time, which is placed in detection slot i.e. using clamping forceps, can be carried out detection, in addition to blood sample, not need to prepare other stage properties.
2, the utility model passes through measurement buffer, lactic dehydrogenase enzymatic protective reagent and oxidation state two nucleoside of nicotinamide adenine
The setting of acid, can complete detection work with these reagents and blood sample can directly wherein by the setting of testing cavity
Detection, does not need to prepare additional detection container.
3, the utility model is by the setting of hinge, detection or it is idle when manufacture closed space, avoid generating dirty
Dye, and by the setting of spring bearing and reel, can drawing, be easy to use, by the setting of magnetic stripe, pull on or altogether
When can firmly suck shell, do not generate big gap.
Detailed description of the invention
Fig. 1 is a kind of lactic dehydrogenase detection of the utility model main view of kit；
Fig. 2 is the internal structure chart of the shell of the utility model；
Fig. 3 is the structural schematic diagram of the hinge of the utility model；
Fig. 4 is the cross-sectional view of the upper cover of the utility model.
In figure: 1, detection slot；2, colour atla；3, protective film；4, upper cover；5, hinge；6, shell；7, buffer is measured；8, newborn
Acidohydrogenase protective agent；9, oxidation state nicotinamide adenine dinucleotide；10, reagent placed cavity；11, guide rail；12, testing cavity；
13, magnetic stripe；14, bracket；15, spring bearing；16, reel；17, hinge；18, clamping forceps；19, medicine film is reacted；20, chamber is put.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.
Fig. 1-4 is please referred to, a kind of embodiment provided by the utility model: a kind of lactic dehydrogenase detection kit, packet
Shell 6 is included, the upper end of shell 6 is equipped with upper cover 4, and hinge 5 is provided between shell 6 and upper cover 4, and the side of 4 upper end of upper cover is set
It is equipped with detection slot 1, carries out simply detected place, the side of detection slot 1 is provided with colour atla 2, and the side of colour atla 2 is provided with protection
Film 3 does not allow dust pollution internal, and the side inside shell 6 is provided with reagent placed cavity 10, and the side in reagent placed cavity 10 is put
It is equipped with oxidation state nicotinamide adenine dinucleotide 9, it is slow that the side of oxidation state nicotinamide adenine dinucleotide 9 is placed with measurement
Fliud flushing 7 and lactic dehydrogenase enzymatic protective reagent 8, and the front end that buffer 7 is placed on lactic dehydrogenase enzymatic protective reagent 8 is measured, these are
The raw material of lactic dehydrogenase detection liquid is made, the other side inside shell 6 is provided with testing cavity 12, the side of 12 upper end of testing cavity
It is provided with guide rail 11, is slided, testing cavity is provided with magnetic stripe 13 on rear side of 12 upper end, can pull on when not in use, with shell 6
Fixed, the upper end of magnetic stripe 13 is provided with hinge 17, and the upper end of hinge 17 is provided with reel 16, is arranged between reel 16 and hinge 17
There is spring bearing 15,17 convolution of hinge is stored, the two sides of reel 16 are provided with bracket 14, the middle position inside upper cover 4
Place is placed with clamping forceps 18, and the side inside upper cover 4, which is provided with, puts chamber 20, puts and is placed with reaction medicine film 19 in chamber 20, right
In not needing accurately to detect Dehydrogenase Content, it is only necessary to which the crowd for understanding lactic dehydrogenase variation tendency uses, unprofessional
Personnel oneself can also compare out as a result, and expend the time it is short, it is easy to operate.
Further, it detection slot 1 and puts the height of chamber 20 and is respectively less than the height of upper cover 4, and the height of detection slot 1 is less than and sets
The height of chamber 20 is put, the size of protective film 3 is greater than the sectional dimension for putting chamber 20, and the top of clamping forceps 18 is arranged in colour atla 2, saves
Save space.
Further, upper cover 4 and shell 6 are made of plastics, and sealing joint strip is provided between upper cover 4 and shell 6, are guaranteed close
Feng Xing, there are two the settings of hinge 5, and hinge 5 is connect with upper cover 4 and shell 6 respectively by fixed screw, and the height of shell 6 is big
In the height of upper cover 4.
Further, there are two the settings of oxidation state nicotinamide adenine dinucleotide 9, the sectional dimension of reagent placed cavity 10 is big
In being provided with reaction tube in the sectional dimension of testing cavity 12, testing cavity 12, when reaction, directly reacts in reaction tube, is not required to
Prepare test tube, and this material can reuse, test tube can be taken down when detecting absorbance using machine, and reaction tube is set
It is equipped with 18.
Further, the thickness of hinge 17 is set as half millimeter, after hinge 17 is less than shell 6 by the diameter after 16 convolution of reel
The thickness at end, and hinge 17 it is adjacent between be provided with connecting line, play the role of being fixedly connected, magnetic stripe 13 and hinge 17 are fixed to be connected
It connects, magnetic stripe 13 can block shell 6 after 17 convolution of hinge, play fixed effect, and the inside of shell 6 is arranged in bracket 14, volume
The length of cylinder 16 is greater than the length of hinge 17, facilitates convolution, the length of hinge 17 and the equal length of magnetic stripe 13.
Further, the bottom end of clamping forceps 18 is set as arc structure, facilitates clamping reaction medicine film 19, and be not easy to reaction
Medicine film 19 generates destruction, and the upper end of clamping forceps 18 is set on mouth structure, can open and take out clamping forceps 18, and upper end is lucky
Colour atla 2 can be bonded, reaction medicine film 19 is provided with several, is fitted and connected between reaction medicine film 19 is adjacent.
Working principle: in use, the reaction medicine film 19 in a piece of storing chamber 20 is taken with clamping forceps 18, reaction medicine film 19 is flat
It is layered in detection slot 1, takes blood sample drop in reaction medicine film 19, since reaction medicine film 19 is combined by lactic dehydrogenase enzyme specificity
The fixative and color developing agent of antibody are constituted, all to have certain chromogenic reaction, are compared according to this colour developing with colour atla 2, are substantially judged
The activity of lactic dehydrogenase in blood sample uses oxygen when needing accurately to measure the activity of lactic dehydrogenase in blood sample
Change state nicotinamide adenine dinucleotide 9 to react with blood sample, and reaction solution can be placed in testing cavity 12, wherein blood
Sample is preferably the serum after being centrifuged, wherein lactate dehydrogenase catalyzed lactic acid and oxidation state nicotinamide adenine dinucleotide 9
Reaction generates pyruvic acid, nicotinamide adenine dinucleotide and hydrogen ion, lactic acid and is oxidized to pyruvic acid, simultaneous oxidation state nicotinoyl
Amine adenine-dinucleotide 9 is oxidized to nicotinamide adenine dinucleotide, causes the raising of absorbance at 340nm, increases speed
Rate is proportional to lactic acid dehydrogenase activity in sample, detects extinction at 340nm by spectrophotometer or automatic analyzer
Increase rate is spent, the activity of lactic dehydrogenase is obtained by calculating.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
Claims (6)
1. a kind of lactic dehydrogenase detection kit, including shell (6), it is characterised in that: install the upper end of the shell (6)
Have upper cover (4), be provided with hinge (5) between the shell (6) and upper cover (4), the side of upper cover (4) upper end is provided with inspection
It surveys slot (1), the side of the detection slot (1) is provided with colour atla (2), and the side of the colour atla (2) is provided with protective film (3), institute
It states the internal side of shell (6) to be provided with reagent placed cavity (10), the side in the reagent placed cavity (10) is placed with oxidation
State nicotinamide adenine dinucleotide (9), it is slow that the side of the oxidation state nicotinamide adenine dinucleotide (9) is placed with measurement
Fliud flushing (7) and lactic dehydrogenase enzymatic protective reagent (8), and the front end that buffer (7) are placed on lactic dehydrogenase enzymatic protective reagent (8) is measured,
The internal other side of the shell (6) is provided with testing cavity (12), and the side of testing cavity (12) upper end is provided with guide rail
(11), it being provided with magnetic stripe (13) on rear side of testing cavity (12) upper end, the upper end of the magnetic stripe (13) is provided with hinge (17),
The upper end of the hinge (17) is provided with reel (16), is provided with spring bearing between the reel (16) and hinge (17)
(15), the two sides of the reel (16) are provided with bracket (14), and the internal middle position of the upper cover (4) is placed with clamping
Tweezer (18), the internal side of the upper cover (4), which is provided with, puts chamber (20), is placed with reaction medicine film in the storing chamber (20)
(19).
2. a kind of lactic dehydrogenase detection kit according to claim 1, it is characterised in that: the detection slot (1)
The height of upper cover (4) is respectively less than with the height for putting chamber (20), and the height of detection slot (1) is less than the height for putting chamber (20),
The size of the protective film (3) is greater than the sectional dimension for putting chamber (20), and the colour atla (2) is arranged in the upper of clamping forceps (18)
Side.
3. a kind of lactic dehydrogenase detection kit according to claim 1, it is characterised in that: the upper cover (4) and
Be provided with sealing joint strip between shell (6), there are two hinge (5) settings, and hinge (5) by fixed screw respectively with it is upper
(4) and shell (6) connection are covered, the height of the shell (6) is greater than the height of upper cover (4).
4. a kind of lactic dehydrogenase detection kit according to claim 1, it is characterised in that: the oxidation state nicotinoyl
There are two amine adenine-dinucleotide (9) settings, and the sectional dimension of the reagent placed cavity (10) is greater than cutting for testing cavity (12)
Face size, the testing cavity (12) is interior to be provided with reaction tube, and reaction tube is provided with 18.
5. a kind of lactic dehydrogenase detection kit according to claim 1, it is characterised in that: the hinge (17)
Thickness is set as half millimeter, and hinge (17) it is adjacent between be provided with connecting line, the magnetic stripe (13) and hinge (17) are fixed connects
It connects, in the inside of shell (6), the length of the reel (16) is greater than the length of hinge (17), described for bracket (14) setting
The length of hinge (17) and the equal length of magnetic stripe (13).
6. a kind of lactic dehydrogenase detection kit according to claim 1, it is characterised in that: the clamping forceps (18)
Bottom end be set as arc structure, and the upper end of clamping forceps (18) is set on mouth structure, and the reaction medicine film (19) is provided with
Several, are fitted and connected between the reaction medicine film (19) is adjacent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821726766.8U CN209148700U (en) | 2018-10-24 | 2018-10-24 | Kit is used in a kind of detection of lactic dehydrogenase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821726766.8U CN209148700U (en) | 2018-10-24 | 2018-10-24 | Kit is used in a kind of detection of lactic dehydrogenase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209148700U true CN209148700U (en) | 2019-07-23 |
Family
ID=67270771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821726766.8U Active CN209148700U (en) | 2018-10-24 | 2018-10-24 | Kit is used in a kind of detection of lactic dehydrogenase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209148700U (en) |
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2018
- 2018-10-24 CN CN201821726766.8U patent/CN209148700U/en active Active
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