CN209113923U - A kind of collagen coating plate for hepatocyte cultures - Google Patents

Abstract

The utility model discloses a kind of collagens for hepatocyte cultures to be coated with plate, including holder, the upper end of holder is equipped with matched box cover, the lower end of box cover is fixedly connected with two piece of first magnet, the upper end of holder is equipped with multiple culture mechanisms, the upper end of holder is correspondingly provided with multiple open-and-close mechanisms for being used to be opened and closed culture mechanism, is equipped with supporting mechanism on the left and right ends side wall of holder, two supporting mechanisms are connect with two piece of first magnet magnetism respectively.The utility model can protect each culture cup when carrying out culture or transfer operation, other foreign matters has been prevented to be splashed into culture cup, while can provide support when operator's operation for its hand, take and put convenient for it.

Description

A kind of collagen coating plate for hepatocyte cultures

Technical field

The utility model relates to be coated with plate technique field more particularly to a kind of collagen for hepatocyte cultures to be coated with plate.

Background technique

Liver is the vitals of human body, metabolism, digestion, removing toxic substances, blood coagulation, it is immune, in terms of play very Important role facilitates the in vitro studies such as pharma-toxicology, immunology, cell biology from the isolated liver cell of body, And the application with bioartificial liver in treatment hepatic failure field, it has increasing need for a large amount of, high activity primary hepatocyte and makees For biomaterial.

But existing hepatocyte cultures collagen coating plate is Integral opening-closing type, easily occurs splashing down contamination when being operated Equal maloperations, cause experiment easily error or other accidents occur, are not easy to experimenter's operation, while making carrying out culture operation When, the hand inconvenience of operator is put, it is made easily to occur shaking or the phenomenon of taking and placing shakiness, is not easy to that experimenter is daily to be made With.

Utility model content

Purpose of the utility model is to solve disadvantages existing in the prior art, and the one kind proposed is used for liver cell The collagen of culture is coated with plate.

To achieve the goals above, the utility model adopts the technical scheme that

A kind of collagen coating plate for hepatocyte cultures, including holder, the upper end of the holder are equipped with phase therewith Matched box cover, the lower end of the box cover are fixedly connected with two piece of first magnet, and the upper end of the holder is equipped with multiple cultures Mechanism, the upper end of the holder are correspondingly provided with multiple for being opened and closed the open-and-close mechanism of culture mechanism, the left and right of the holder Supporting mechanism is equipped on the side wall of both ends, two supporting mechanisms are connect with two piece of first magnet magnetism respectively.

Preferably, the culture mechanism includes the culture tank for being set to holder upper end, is equipped with therewith in the culture tank The bottom of the culture cup to match, the culture cup is coated with collagen layer.

Preferably, the open-and-close mechanism includes the first rotating shaft for being rotationally connected with holder upper end, the first rotating shaft The switchboard to match with culture tank is socketed on side wall, the switchboard is located at the surface of culture tank, the switchboard Lower end is equipped with clamping mechanism, and the upper end of the holder is equipped with hemispherical card slot corresponding with clamping mechanism.

Preferably, the clamping mechanism includes the groove for being set to switchboard lower end, and the top surface of the groove is fixedly connected There are more telescopic rods, the more telescopic rods are fixedly connected with same hemispherical fixture block far from one end of groove top surface, described Hemispherical fixture block and hemispherical card slot are cooperatively connected, and are arranged with spring on the telescopic rod.

Preferably, the upper end of the switchboard is equipped with burr layer.

Preferably, the supporting mechanism includes the opening being set on holder side wall, is equipped with second turn in the opening Axis, the rear and front end of second shaft are rotatablely connected with the rear and front end inner wall of opening respectively, the side wall of second shaft On be fixedly connected with support plate, the upper end of the support plate is fixedly connected with foam-rubber cushion, and the lower end of the support plate is fixedly connected There is support column, the lower end of the support column is fixedly connected with the second magnet, and second magnet is connect with the first magnet magnetism.

Compared with prior art, the utility model has the following beneficial effects:

1, by the cooperation of the switchboard of setting and clamping mechanism and hemispherical card slot, with reach can be by individually rotating out The mode of plate is closed to protect to each culture cup, prevents having foreign matter to fall pollution culture medium when carrying out batch operation, And culture cup can be drawn off shifting at any time convenient for experimenter, while its switch operation is convenient that experimenter is facilitated to use.

2, by the first magnet of setting and the cooperation of supporting mechanism, to reach when in use, experimenter can will be supported Plate rotating opening simultaneously simply supports it by support column, convenient for keeping the stabilization of hand, while sea in operation Comfort when hand relies on can be improved in silk floss pad, and obtaining magnet can cooperate the first magnet firmly to adsorb box cover, improves Its tautness when closing holder.

Detailed description of the invention

Fig. 1 be the utility model proposes a kind of collagen for hepatocyte cultures be coated with the overlooking structure diagram of plate；

Fig. 2 is the elevational perspective view of Fig. 1；

Fig. 3 is the partial enlarged view in Fig. 2 at A.

In figure: 1 holder, 2 box covers, 3 first magnet, 4 culture mechanisms, 5 open-and-close mechanisms, 6 supporting mechanisms, 7 culture tanks, 8 Culture cup, 9 collagen layers, 10 first rotating shafts, 11 switchboards, 12 clamping mechanisms, 13 telescopic rods, 14 hemispherical fixture blocks, 15 springs, 16 Second shaft, 17 support plates, 18 foam-rubber cushions, 19 support columns, 20 second magnet.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.

Referring to Fig.1-3, a kind of collagen for hepatocyte cultures is coated with plate, including holder 1, the upper end of holder 1 are set There is matched box cover 2, the lower end of box cover 2 is fixedly connected with two piece of first magnet 3, and the upper end of holder 1 is equipped with multiple Mechanism 4 is cultivated, culture mechanism 4 includes the culture tank 7 for being set to 1 upper end of holder, multiple 7 side by side parallel of culture tank settings, training It supports in slot 7 and is placed with matched culture cup 8, the bottom of culture cup 8 is coated with collagen layer 9.

The upper end of holder 1 is correspondingly provided with multiple open-and-close mechanisms 5 for being used to be opened and closed culture mechanism 4, and open-and-close mechanism 5 includes turning The first rotating shaft 10 for being connected to 1 upper end of holder is moved, is socketed with the switch to match with culture tank 7 on the side wall of first rotating shaft 10 Plate 11, the upper end of switchboard 11 are equipped with burr layer in order to increase the frictional force with operator's hand, convenient for opening it, open The surface that plate 11 is located at culture tank 7 is closed, the lower end of switchboard 11 is equipped with clamping mechanism 12, and the upper end of holder 1 is equipped with and card The corresponding hemispherical card slot of tight mechanism 12, clamping mechanism 12 include the groove for being set to 11 lower end of switchboard, the top surface of groove More telescopic rods 13 are fixedly connected with, more telescopic rods 13 are fixedly connected with same hemispherical card far from one end of groove top surface Block 14, hemispherical fixture block 14 and hemispherical card slot are cooperatively connected, and are arranged with spring 15 on telescopic rod 13, telescopic rod 13 can be to spring 15 are protected, and upper end of the upper and lower ends of spring 15 respectively with the top surface of groove and hemispherical fixture block 14 abuts, spring 15 are used for hemispherical fixture block 14 and the clamping of hemispherical card slot.

Be equipped with supporting mechanism 6 on the left and right ends side wall of holder 1, two supporting mechanisms 6 respectively with two piece of first magnetic The magnetic connection of iron 3, supporting mechanism 6 include the opening being set on 1 side wall of holder, are equipped with the second shaft 16 in opening, and second The rear and front end of shaft 16 is rotatablely connected with the rear and front end inner wall of opening respectively, is fixedly connected on the side wall of the second shaft 16 Support plate 17, the upper end of support plate 17 are fixedly connected with foam-rubber cushion 18, and foam-rubber cushion 18 can be improved when operator's hand relies on Comfort, the lower end of support plate 17 are fixedly connected with support column 19, and the lower end of support column 19 is fixedly connected with the second magnet 20, the Two magnet 20 are connect with 3 magnetism of the first magnet, and the second magnet 20 can improve it with the cooperation of the first magnet 3 when box cover 2 is closed and close The tautness of conjunction.

The utility model, operator is first placed on holder 1 is safe on testing stand when in use, then by box cover 2 upward pick up are removed, and two piece of second magnet 20 is separated with two piece of first magnet 3 during taking, and operator is rotated further by Support plate 17 makes it lay flat and support using the second magnet 20 and support column 19, during operation, operation The hand of personnel can be prevented to keep firm on foam-rubber cushion 18, operator can by way of rotating switch plate 11 by Multiple culture tanks 7 are opened, and experiment is transferred on the collagen layer 9 in culture cup with object, during opening, hemispherical fixture block 14 extruding springs 15 make its contraction, when closed, under the action of spring 15, hemispherical fixture block 14 again with hemispherical card slot card It connects to close culture tank 7 and be protected to internal collagen layer 9, operator can will pass through culture when being shifted Cup 8 completes transfer tasks, and after the completion of experiment, the rotation of support plate 17 is retracted in holder 1 by operator again, by box cover 2 cover, so that the second magnet 20 is connect with the first magnet 3 again magnetism, to improve its tautness when closing holder 1.

The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model Within enclosing.

Claims (6)

1. a kind of collagen for hepatocyte cultures is coated with plate, including holder (1), which is characterized in that the holder (1) Upper end is equipped with matched box cover (2), and the lower end of the box cover (2) is fixedly connected with two piece of first magnet (3), described to put The upper end for setting box (1) is equipped with multiple culture mechanisms (4), and the upper end of the holder (1) is correspondingly provided with multiple for being opened and closed culture The open-and-close mechanism (5) of mechanism (4) is equipped with supporting mechanism (6) on the left and right ends side wall of the holder (1), described in two Supporting mechanism (6) is connect with two piece of first magnet (3) magnetism respectively.

2. a kind of collagen for hepatocyte cultures according to claim 1 is coated with plate, which is characterized in that the culture machine Structure (4) includes the culture tank (7) for being set to holder (1) upper end, is equipped with matched culture cup in the culture tank (7) (8), the bottom of the culture cup (8) is coated with collagen layer (9).

3. a kind of collagen for hepatocyte cultures according to claim 2 is coated with plate, which is characterized in that the opening-closing machine Structure (5) includes the first rotating shaft (10) for being rotationally connected with holder (1) upper end, is socketed on the side wall of the first rotating shaft (10) The switchboard (11) to match with culture tank (7), the switchboard (11) are located at the surface of culture tank (7), the switchboard (11) lower end is equipped with clamping mechanism (12), and the upper end of the holder (1) is equipped with hemisphere corresponding with clamping mechanism (12) Shape card slot.

4. a kind of collagen for hepatocyte cultures according to claim 3 is coated with plate, which is characterized in that the clamping machine Structure (12) includes the groove for being set to switchboard (11) lower end, and the top surface of the groove is fixedly connected with more telescopic rods (13), The more telescopic rods (13) are fixedly connected with same hemispherical fixture block (14), the hemispherical far from one end of groove top surface Fixture block (14) and hemispherical card slot are cooperatively connected, and are arranged with spring (15) on the telescopic rod (13).

5. a kind of collagen for hepatocyte cultures according to claim 3 is coated with plate, which is characterized in that the switchboard (11) upper end is equipped with burr layer.

6. a kind of collagen for hepatocyte cultures according to claim 1 is coated with plate, which is characterized in that the support machine Structure (6) includes the opening being set on holder (1) side wall, is equipped with the second shaft (16) in the opening, second shaft (16) rear and front end is rotatablely connected with the rear and front end inner wall of opening respectively, fixed on the side wall of second shaft (16) to connect It is connected to support plate (17), the upper end of the support plate (17) is fixedly connected with foam-rubber cushion (18), the lower end of the support plate (17) It is fixedly connected with support column (19), the lower end of the support column (19) is fixedly connected with the second magnet (20), second magnet (20) it is connect with the first magnet (3) magnetism.