CN209027889U - Gram's staining device - Google Patents
Gram's staining device Download PDFInfo
- Publication number
- CN209027889U CN209027889U CN201821388988.3U CN201821388988U CN209027889U CN 209027889 U CN209027889 U CN 209027889U CN 201821388988 U CN201821388988 U CN 201821388988U CN 209027889 U CN209027889 U CN 209027889U
- Authority
- CN
- China
- Prior art keywords
- staining
- dyeing
- gram
- zone
- rinsing zone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000010186 staining Methods 0.000 title claims abstract description 16
- 238000004043 dyeing Methods 0.000 claims abstract description 40
- 239000011521 glass Substances 0.000 claims abstract description 26
- 239000002699 waste material Substances 0.000 claims abstract description 15
- 238000005192 partition Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000005406 washing Methods 0.000 claims description 25
- 238000003794 Gram staining Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 125000006850 spacer group Chemical group 0.000 claims description 12
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000011010 flushing procedure Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000000975 dye Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- VBIXEXWLHSRNKB-UHFFFAOYSA-N ammonium oxalate Chemical compound [NH4+].[NH4+].[O-]C(=O)C([O-])=O VBIXEXWLHSRNKB-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000003235 crystal violet staining Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of Gram's staining devices, wherein, the Gram's staining device includes that upper surface is formed as open, and the rinsing area (1) of inner hollow, and at least one side wall from the rinsing area (1) extend outward to form at least one dyeing area of the trough body structure for surface open；Wherein, the rest area that can place glass slide (9) is formed in the rinsing area (1)；The inside in the dyeing area is horizontally disposed partition (8), so that the dyeing is distinguished every being formed as the upper half and lower half, and at least one socket (6) is provided on the partition (8) along the vertical direction, and glass slide (9) can be placed through the socket (6).Realize the use function including a variety of equipment, and waste liquid etc. is more convenient for collecting, at the same glass slide put it is more regular and convenient for operation so that its is easy to use while also saving the effect of the time before experiment.
Description
Technical Field
The utility model relates to a gram stain field specifically, relates to gram staining device.
Background
The gram staining method is the most basic and important differential staining technology in bacteriology, and is one of the most common methods used by vast microorganism researchers in microorganism basic experiments. The existing gram staining apparatus is suitable for single operation, and is difficult to realize or even incapable of realizing the operation of dyeing for multiple times under one device by one person, and the existing gram staining equipment is scattered and numerous, so that the apparatus is very inconvenient to use, and the apparatus is often required to be collected in a centralized manner and then stored during arrangement, so that the operator is very inconvenient to take and store, and the apparatus is easy to be omitted carelessly during use.
Therefore, provide a service function including multiple equipment, and waste liquid etc. are more convenient for collect, and putting of slide glass is more regular and the operation of being convenient for simultaneously for its convenient to use has also practiced thrift the gram staining device of the preparation time before the experiment simultaneously and is the utility model discloses the problem that needs solve urgently.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned prior art, an object of the utility model is to provide an in service function including multiple equipment, and the waste liquid etc. are more convenient for collect, and putting of slide glass is more regular and the operation of being convenient for simultaneously for its convenient to use has also practiced thrift the gram staining device of the preparation time before the experiment simultaneously.
In order to accomplish the above objects, the present invention provides a gram staining apparatus, wherein the gram staining apparatus comprises a washing zone formed to be open on an upper surface and hollow inside, and at least one staining zone formed to be a groove structure open on a surface extending outward from at least one sidewall of the washing zone; wherein,
a placing area capable of placing a glass slide is formed in the washing area;
the inside in dyeing district is provided with the spacer along the horizontal direction to make the dyeing district separates to form into upper half and lower half, just be provided with at least one socket along vertical direction on the spacer, and the slide glass can run through the socket is placed.
Preferably, a partition plate is arranged in the flushing area along the vertical direction, and the distance between the upper surface of the partition plate and the flushing area is smaller than the height of the side wall of the flushing area.
Preferably, a plate surface is formed by connecting the upper surface of the partition plate and the upper surface of the side wall of the flushing area, and at least one groove is formed on the plate surface in an inward concave manner.
Preferably, at least one first recess is formed on the upper surface of the side wall of the washing area, a plurality of second recesses matched with the first recesses are formed on the upper surface of the partition board, each first recess and the corresponding second recess form a placing groove, and the glass slides can be clamped and placed in the placing grooves.
Preferably, the dyeing zone comprises a first dyeing part and a second dyeing part which extend along one side surface of the washing zone in sequence, and a third dyeing part which extends along the opposite side surface.
Preferably, the bottom of the side of the first dyeing part, the second dyeing part and the third dyeing part is also provided with a water outlet, and a rubber plug is detachably arranged on the water outlet.
Preferably, the water outlet is also communicated with a waste liquid collecting unit.
Preferably, the waste liquid collecting unit at least comprises a through pipe and a waste liquid barrel which are sequentially communicated with each other from the water outlet.
According to the technical scheme, the utility model discloses a set up the flushing area, and be formed with the district of placing that can place the slide glass in the flushing area, make can arrange the slide glass in and place fixed washing in the district, avoid washing inadequately or careless landing leads to the broken scheduling problem of slide glass in the normal handheld cleaning process, furthermore, set up the spacer along the horizontal direction in the inside in dyeing district, and set up the socket along vertical direction on the spacer, make the slide glass can run through the socket and place, and simultaneously, add the dyestuff in the dyeing district, can dye it simultaneously, can save the dyeing time, also can further guarantee the homogeneity of dyeing. And the device comprises parts required in the whole operation process of gram staining, so that the device can be conveniently operated and tidied, and the working efficiency is greatly improved.
Other features and advantages of the present invention will be described in detail in the detailed description which follows.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural diagram of a gram staining apparatus provided by the present invention.
Description of the reference numerals
1-rinsing zone 2-first dyeing section
3-second dyeing section 4-third dyeing section
5-groove 6-socket
7-Water Outlet 8-spacer
9-slide 10-spacer.
Detailed Description
The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings. It is to be understood that the description of the embodiments herein is for purposes of illustration and explanation only and is not intended to limit the invention.
In the present invention, unless otherwise specified, the terms of orientation such as "lateral, upper, lower, bottom, vertical, horizontal" and the like included in the terms only represent the orientation of the terms in a conventional use state or are colloquially known by those skilled in the art, and should not be construed as limiting the terms.
The utility model provides a gram staining device, wherein, as shown in figure 1, the gram staining device comprises a washing area 1 with an open upper surface and a hollow interior, and at least one staining area which extends outwards from at least one side wall of the washing area 1 and is formed into a groove body structure with an open surface; wherein,
a placing area capable of placing a slide glass 9 is formed in the washing area 1;
the inside of the staining area is provided with a spacer 8 along the horizontal direction, so that the staining area is divided into an upper half part and a lower half part, at least one inserting opening 6 is arranged on the spacer 8 along the vertical direction, and a glass slide 9 can be placed through the inserting opening 6.
The utility model discloses a set up flushing area 1, and be formed with the district of placing that can place slide glass 9 in flushing area 1, make and to arrange slide glass 9 in and place fixed washing in the district, avoid washing inadequately or careless landing leads to the broken scheduling problem of slide glass 9 in the normal handheld cleaning process, furtherly, set up spacer 8 along the horizontal direction in the inside in dyeing district, and set up socket 6 along vertical direction on spacer 8, make slide glass 9 can run through the socket and place, and simultaneously, add the dyestuff in the dyeing district, can dye it simultaneously, can save the dyeing time, also can further guarantee the homogeneity of dyeing. And the device comprises parts required in the whole operation process of gram staining, so that the device can be conveniently operated and tidied, and the working efficiency is greatly improved.
In a preferred embodiment of the present invention, in order to make it possible to place the slide glass 9 at a certain inclination angle, so that the waste liquid after washing can better fall into the washing area, avoid the pollution to the surrounding environment, and make the cleaning at its later stage convenient, a partition plate 10 is provided in the washing area 1 along the vertical direction, and the upper surface of the partition plate 10 and the distance between the washing areas 1 are smaller than the height of the sidewall of the washing area 1. So that the slide 9 can be set up between the side wall of the washing zone 1 and the partition 10, making it assume an inclination, and so that the washing waste water flows down to the partition 10 and into the washing zone 1.
In a further preferred embodiment, in order to make the placement of the partition plate more stable, the upper surface of the partition plate 10 and the upper surface of the side wall of the flushing area 1 are connected to form a plate surface, and the plate surface is recessed inwards to form at least one groove 5.
In another preferred embodiment, at least one first recess is formed on the upper surface of the sidewall of the washing area 1, a plurality of second recesses matching with the first recesses are formed on the upper surface of the partition board 10, and each first recess and the corresponding second recess form a placement slot in which the slide glass 9 can be placed in a snap fit. That is, one end of the slide glass 9 is engaged with the first recess, and the other end is engaged with the mating second recess.
The number of the dyeing zones can be set according to actual needs, for example, in a preferred embodiment, the dyeing zone comprises a first dyeing part 2 and a second dyeing part 3 which are sequentially extended along one side surface of the washing zone 1, and a third dyeing part 4 which is extended along the opposite side surface.
In a further preferred embodiment, in order to facilitate the collection and treatment of the dyed waste liquid, a water outlet 7 is further disposed at the bottom of the side surface of the first dyeing part 2, the second dyeing part 3 and the third dyeing part 4, and a rubber plug is detachably disposed on the water outlet 7.
In order to facilitate the collection and treatment of the waste liquid after dyeing, the utility model discloses an optimized embodiment, still be communicated with the waste liquid collection unit on the delivery port 7.
In a further preferred embodiment, the waste liquid collecting unit at least comprises a through pipe and a waste liquid barrel which are sequentially communicated with each other from the water outlet 7.
The following description will be specifically made in conjunction with the gram staining method.
Fixing a smear: the slides 9 are placed on the upper surface of the staining area, and after a drop of physiological saline is dropped, six slides 9 are smeared one by one with an inoculating loop. Then drying and fixing the bacterial colony above the flame of the alcohol lamp.
Primary dyeing: six glass slides 9 are respectively inserted into the insertion openings 6 of the spacers 8 of the first staining part 2 (namely, the ammonium oxalate crystal violet staining solution), the glass slides 9 are taken out to be placed on the positions of each group of grooves 5 in the washing area 1 after the staining is performed for a certain time, and then each glass slide 9 is washed by a water washing bottle.
Mordant dyeing: the resultant was placed in the second dyeing section 3 (i.e., iodine solution) to be dyed in the same manner as above.
And (3) decoloring: in the rinsing zone 1.
Counterdyeing: the obtained product is placed in the third dyeing part 4 (i.e. safranin dyeing solution), and the rest of the operation method is the same as the above.
The preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, however, the present invention is not limited to the details of the above embodiments, and the technical concept of the present invention can be within the scope of the present invention to perform various simple modifications to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and in order to avoid unnecessary repetition, the present invention does not need to describe any combination of the features.
In addition, various embodiments of the present invention can be combined arbitrarily, and the disclosed content should be regarded as the present invention as long as it does not violate the idea of the present invention.
Claims (8)
1. A gram staining apparatus, characterized in that it comprises a rinsing zone (1) formed open on its upper surface and hollow inside, and at least one staining zone extending outwardly from at least one side wall of the rinsing zone (1) forming a surface-open channel structure; wherein,
a placing area capable of placing a slide glass (9) is formed in the washing area (1);
the inside of dyeing district is provided with spacer (8) along the horizontal direction to make the dyeing district partition form into upper half and lower half, and be provided with at least one socket (6) along vertical direction on spacer (8), and slide glass (9) can run through the socket (6) are placed.
2. A gram stain apparatus according to claim 1, wherein a baffle (10) is arranged in the rinsing zone (1) in a vertical direction and the distance between the upper surface of the baffle (10) and the rinsing zone (1) is smaller than the height of the side wall of the rinsing zone (1).
3. A gram stain apparatus as claimed in claim 2, wherein the connection between the upper surface of the baffle (10) and the upper surface of the side wall of the rinsing zone (1) is formed as a plate surface, and the plate surface is formed with at least one groove (5) recessed inwards.
4. A gram stain apparatus as claimed in claim 2, wherein the side wall of the rinsing zone (1) is formed with at least one first recess on its upper surface, the spacer (10) is formed with a plurality of second recesses on its upper surface which mate with the first recesses, and each first recess and the corresponding second recess form a placement slot in which the slide (9) can be snap-placed.
5. A gram stain apparatus as claimed in claim 1 or 2, wherein the staining zone comprises first (2) and second (3) staining sections extending sequentially along one side of the rinsing zone (1) and a third staining section (4) extending along the opposite side.
6. A gram staining device according to claim 5, wherein the first, second and third staining parts (2, 3, 4) are further provided with a water outlet (7) on the bottom of their side faces, and wherein a rubber stopper is detachably provided on the water outlet (7).
7. A gram stain apparatus as claimed in claim 6, wherein the water outlet (7) is also connected to a waste liquid collection unit.
8. A gram stain apparatus as claimed in claim 7, wherein the waste collection unit comprises at least a waste barrel and a duct communicating in series from the water outlet (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821388988.3U CN209027889U (en) | 2018-08-27 | 2018-08-27 | Gram's staining device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821388988.3U CN209027889U (en) | 2018-08-27 | 2018-08-27 | Gram's staining device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209027889U true CN209027889U (en) | 2019-06-25 |
Family
ID=66875506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821388988.3U Expired - Fee Related CN209027889U (en) | 2018-08-27 | 2018-08-27 | Gram's staining device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209027889U (en) |
-
2018
- 2018-08-27 CN CN201821388988.3U patent/CN209027889U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190625 Termination date: 20200827 |